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Protein

3-isopropylmalate dehydratase large subunit

Gene

leuC

Organism
Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate.UniRule annotation

Catalytic activityi

(2R,3S)-3-isopropylmalate = (2S)-2-isopropylmalate.UniRule annotation

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster per subunit.UniRule annotation

Pathwayi: L-leucine biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes L-leucine from 3-methyl-2-oxobutanoate.UniRule annotation
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. 3-isopropylmalate dehydratase large subunit (leuC), 3-isopropylmalate dehydratase small subunit (leuD)
  3. no protein annotated in this organism
  4. no protein annotated in this organism
This subpathway is part of the pathway L-leucine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-leucine from 3-methyl-2-oxobutanoate, the pathway L-leucine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi262Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi320Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi323Iron-sulfur (4Fe-4S)UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyase
Biological processAmino-acid biosynthesis, Branched-chain amino acid biosynthesis, Leucine biosynthesis
Ligand4Fe-4S, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

BioCyciPHOR70601:G1G39-1668-MONOMER
UniPathwayiUPA00048; UER00071

Names & Taxonomyi

Protein namesi
Recommended name:
3-isopropylmalate dehydratase large subunitUniRule annotation (EC:4.2.1.33UniRule annotation)
Alternative name(s):
Alpha-IPM isomeraseUniRule annotation
Short name:
IPMIUniRule annotation
Isopropylmalate isomeraseUniRule annotation
Gene namesi
Name:leuCUniRule annotation
Ordered Locus Names:PH1726
OrganismiPyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Taxonomic identifieri70601 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
Proteomesi
  • UP000000752 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000768821 – 3803-isopropylmalate dehydratase large subunitAdd BLAST380

Interactioni

Subunit structurei

Heterodimer of LeuC and LeuD.UniRule annotation

Protein-protein interaction databases

STRINGi70601.PH1726

Structurei

3D structure databases

ProteinModelPortaliO59391
SMRiO59391
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the aconitase/IPM isomerase family. LeuC type 2 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiarCOG01698 Archaea
COG0065 LUCA
HOGENOMiHOG000226971
KOiK01703
OMAiGCGPCMG
OrthoDBiPOG093Z03G6

Family and domain databases

CDDicd01583 IPMI, 1 hit
Gene3Di3.30.499.10, 1 hit
HAMAPiMF_01027 LeuC_type2, 1 hit
InterProiView protein in InterPro
IPR015931 Acnase/IPM_dHydase_lsu_aba_1/3
IPR001030 Acoase/IPM_deHydtase_lsu_aba
IPR018136 Aconitase_4Fe-4S_BS
IPR036008 Aconitase_4Fe-4S_dom
IPR011826 HAcnase/IPMdehydase_lsu_prok
IPR033941 IPMI_cat
PfamiView protein in Pfam
PF00330 Aconitase, 2 hits
PRINTSiPR00415 ACONITASE
SUPFAMiSSF53732 SSF53732, 1 hit
TIGRFAMsiTIGR02086 IPMI_arch, 1 hit
PROSITEiView protein in PROSITE
PS00450 ACONITASE_1, 1 hit
PS01244 ACONITASE_2, 1 hit

Sequencei

Sequence statusi: Complete.

O59391-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTLVEEILDA KAGEVVIRNV DLVYAHDGTM PLIIEAFNKV FNSVKARAYV
60 70 80 90 100
FFDHVYPAPT VKVANLQKEI REFARRHGIP VVEGKGISHQ LVVEMGLAEK
110 120 130 140 150
SRIVVGGDSH TPTLGALGVF AVGMGATDVA VVLGLGKTWL RVPESVAVIF
160 170 180 190 200
EGKPSRTTMA ADVMIRLITS LRNYDMNYKA LEFFNVPFTA DERLTLTNFS
210 220 230 240 250
VEANAKTALI GEEYDGRNYI KEISFELSSL GPMVAKPFSP ANGVEVEKVE
260 270 280 290 300
GTKIDQVFIG SCTNGRFEHI KRAAEILKGE EVAVRTIIGP ASVNVYKRMV
310 320 330 340 350
REGIVDILLD AGATILPPGC GPCLGRHMGV LGDKEVVLST TNRNFRGRMG
360 370 380
SPTAEIYLAS PLTAAVSALY GEITSPGGEV
Length:380
Mass (Da):41,054
Last modified:August 1, 1998 - v1
Checksum:i05BBAA1227B59E9A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000001 Genomic DNA Translation: BAA30840.1
PIRiA71181
RefSeqiWP_010885789.1, NC_000961.1

Genome annotation databases

EnsemblBacteriaiBAA30840; BAA30840; BAA30840
GeneIDi1442570
KEGGipho:PH1726

Similar proteinsi

Entry informationi

Entry nameiLEUC_PYRHO
AccessioniPrimary (citable) accession number: O59391
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 26, 2002
Last sequence update: August 1, 1998
Last modified: April 25, 2018
This is version 114 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health