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Protein

3-isopropylmalate dehydratase large subunit

Gene

leuC

Organism
Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate.UniRule annotation

Catalytic activityi

(2R,3S)-3-isopropylmalate = (2S)-2-isopropylmalate.UniRule annotation

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster per subunit.UniRule annotation

Pathwayi: L-leucine biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes L-leucine from 3-methyl-2-oxobutanoate.UniRule annotation
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. 3-isopropylmalate dehydratase large subunit (leuC), 3-isopropylmalate dehydratase small subunit (leuD)
  3. no protein annotated in this organism
  4. no protein annotated in this organism
This subpathway is part of the pathway L-leucine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-leucine from 3-methyl-2-oxobutanoate, the pathway L-leucine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi262 – 2621Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi320 – 3201Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi323 – 3231Iron-sulfur (4Fe-4S)UniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Amino-acid biosynthesis, Branched-chain amino acid biosynthesis, Leucine biosynthesis

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

BioCyciPHOR70601:GJWR-1723-MONOMER.
UniPathwayiUPA00048; UER00071.

Names & Taxonomyi

Protein namesi
Recommended name:
3-isopropylmalate dehydratase large subunitUniRule annotation (EC:4.2.1.33UniRule annotation)
Alternative name(s):
Alpha-IPM isomeraseUniRule annotation
Short name:
IPMIUniRule annotation
Isopropylmalate isomeraseUniRule annotation
Gene namesi
Name:leuCUniRule annotation
Ordered Locus Names:PH1726
OrganismiPyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Taxonomic identifieri70601 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
Proteomesi
  • UP000000752 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 3803803-isopropylmalate dehydratase large subunitPRO_0000076882Add
BLAST

Interactioni

Subunit structurei

Heterodimer of LeuC and LeuD.UniRule annotation

Protein-protein interaction databases

STRINGi70601.PH1726.

Structurei

3D structure databases

ProteinModelPortaliO59391.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the aconitase/IPM isomerase family. LeuC type 2 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiarCOG01698. Archaea.
COG0065. LUCA.
HOGENOMiHOG000226971.
KOiK01703.
OMAiKNRMGVG.

Family and domain databases

Gene3Di3.30.499.10. 2 hits.
3.40.1060.10. 1 hit.
HAMAPiMF_01027. LeuC_type2.
InterProiIPR015931. Acnase/IPM_dHydase_lsu_aba_1/3.
IPR015937. Acoase/IPM_deHydtase.
IPR001030. Acoase/IPM_deHydtase_lsu_aba.
IPR015932. Aconitase/IPMdHydase_lsu_aba_2.
IPR018136. Aconitase_4Fe-4S_BS.
IPR011826. HAcnase/IPMdehydase_lsu_prok.
[Graphical view]
PANTHERiPTHR11670. PTHR11670. 1 hit.
PfamiPF00330. Aconitase. 2 hits.
[Graphical view]
PRINTSiPR00415. ACONITASE.
SUPFAMiSSF53732. SSF53732. 1 hit.
TIGRFAMsiTIGR02086. IPMI_arch. 1 hit.
PROSITEiPS00450. ACONITASE_1. 1 hit.
PS01244. ACONITASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O59391-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTLVEEILDA KAGEVVIRNV DLVYAHDGTM PLIIEAFNKV FNSVKARAYV
60 70 80 90 100
FFDHVYPAPT VKVANLQKEI REFARRHGIP VVEGKGISHQ LVVEMGLAEK
110 120 130 140 150
SRIVVGGDSH TPTLGALGVF AVGMGATDVA VVLGLGKTWL RVPESVAVIF
160 170 180 190 200
EGKPSRTTMA ADVMIRLITS LRNYDMNYKA LEFFNVPFTA DERLTLTNFS
210 220 230 240 250
VEANAKTALI GEEYDGRNYI KEISFELSSL GPMVAKPFSP ANGVEVEKVE
260 270 280 290 300
GTKIDQVFIG SCTNGRFEHI KRAAEILKGE EVAVRTIIGP ASVNVYKRMV
310 320 330 340 350
REGIVDILLD AGATILPPGC GPCLGRHMGV LGDKEVVLST TNRNFRGRMG
360 370 380
SPTAEIYLAS PLTAAVSALY GEITSPGGEV
Length:380
Mass (Da):41,054
Last modified:August 1, 1998 - v1
Checksum:i05BBAA1227B59E9A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000001 Genomic DNA. Translation: BAA30840.1.
PIRiA71181.
RefSeqiWP_010885789.1. NC_000961.1.

Genome annotation databases

EnsemblBacteriaiBAA30840; BAA30840; BAA30840.
GeneIDi1442570.
KEGGipho:PH1726.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000001 Genomic DNA. Translation: BAA30840.1.
PIRiA71181.
RefSeqiWP_010885789.1. NC_000961.1.

3D structure databases

ProteinModelPortaliO59391.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi70601.PH1726.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAA30840; BAA30840; BAA30840.
GeneIDi1442570.
KEGGipho:PH1726.

Phylogenomic databases

eggNOGiarCOG01698. Archaea.
COG0065. LUCA.
HOGENOMiHOG000226971.
KOiK01703.
OMAiKNRMGVG.

Enzyme and pathway databases

UniPathwayiUPA00048; UER00071.
BioCyciPHOR70601:GJWR-1723-MONOMER.

Family and domain databases

Gene3Di3.30.499.10. 2 hits.
3.40.1060.10. 1 hit.
HAMAPiMF_01027. LeuC_type2.
InterProiIPR015931. Acnase/IPM_dHydase_lsu_aba_1/3.
IPR015937. Acoase/IPM_deHydtase.
IPR001030. Acoase/IPM_deHydtase_lsu_aba.
IPR015932. Aconitase/IPMdHydase_lsu_aba_2.
IPR018136. Aconitase_4Fe-4S_BS.
IPR011826. HAcnase/IPMdehydase_lsu_prok.
[Graphical view]
PANTHERiPTHR11670. PTHR11670. 1 hit.
PfamiPF00330. Aconitase. 2 hits.
[Graphical view]
PRINTSiPR00415. ACONITASE.
SUPFAMiSSF53732. SSF53732. 1 hit.
TIGRFAMsiTIGR02086. IPMI_arch. 1 hit.
PROSITEiPS00450. ACONITASE_1. 1 hit.
PS01244. ACONITASE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3.

Entry informationi

Entry nameiLEUC_PYRHO
AccessioniPrimary (citable) accession number: O59391
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 26, 2002
Last sequence update: August 1, 1998
Last modified: November 11, 2015
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.