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Protein

ADP-specific phosphofructokinase

Gene

pfkC

Organism
Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the phosphorylation of fructose 6-phosphate to fructose 1,6-bisphosphate using ADP as the phosphate donor.UniRule annotation

Catalytic activityi

ADP + D-fructose 6-phosphate = AMP + D-fructose 1,6-bisphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 1 Mg2+ ion per subunit.UniRule annotation

Pathwayi: glycolysis

This protein is involved in the pathway glycolysis, which is part of Carbohydrate degradation.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi260MagnesiumUniRule annotation1
Metal bindingi290MagnesiumUniRule annotation1
Active sitei433Proton acceptorUniRule annotation1
Metal bindingi433MagnesiumUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BRENDAi2.7.1.146. 5244.
UniPathwayiUPA00109.

Names & Taxonomyi

Protein namesi
Recommended name:
ADP-specific phosphofructokinaseUniRule annotation (EC:2.7.1.146UniRule annotation)
Alternative name(s):
ADP-dependent phosphofructokinaseUniRule annotation
Short name:
ADP-PfkUniRule annotation
Gene namesi
Name:pfkCUniRule annotation
Ordered Locus Names:PH1645
OrganismiPyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Taxonomic identifieri70601 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
Proteomesi
  • UP000000752 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001847671 – 450ADP-specific phosphofructokinaseAdd BLAST450

Interactioni

Protein-protein interaction databases

IntActiO59355. 1 interactor.
MINTiMINT-1502590.
STRINGi70601.PH1645.

Structurei

Secondary structure

1450
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi7 – 12Combined sources6
Beta strandi15 – 21Combined sources7
Helixi24 – 32Combined sources9
Helixi36 – 45Combined sources10
Beta strandi48 – 52Combined sources5
Helixi53 – 66Combined sources14
Beta strandi70 – 74Combined sources5
Helixi79 – 86Combined sources8
Beta strandi90 – 96Combined sources7
Helixi97 – 107Combined sources11
Beta strandi111 – 115Combined sources5
Helixi122 – 125Combined sources4
Beta strandi132 – 146Combined sources15
Helixi147 – 149Combined sources3
Beta strandi159 – 165Combined sources7
Beta strandi170 – 175Combined sources6
Beta strandi177 – 179Combined sources3
Beta strandi184 – 190Combined sources7
Helixi194 – 196Combined sources3
Turni202 – 204Combined sources3
Helixi205 – 207Combined sources3
Helixi208 – 214Combined sources7
Beta strandi216 – 220Combined sources5
Helixi223 – 225Combined sources3
Helixi236 – 252Combined sources17
Beta strandi256 – 260Combined sources5
Helixi267 – 276Combined sources10
Helixi278 – 280Combined sources3
Beta strandi281 – 287Combined sources7
Helixi288 – 298Combined sources11
Helixi301 – 310Combined sources10
Helixi313 – 327Combined sources15
Beta strandi330 – 335Combined sources6
Beta strandi337 – 345Combined sources9
Helixi352 – 372Combined sources21
Helixi378 – 380Combined sources3
Helixi381 – 385Combined sources5
Helixi393 – 406Combined sources14
Beta strandi412 – 418Combined sources7
Helixi431 – 447Combined sources17

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1U2XX-ray2.00A/B1-450[»]
3DRWX-ray1.90A/B1-450[»]
ProteinModelPortaliO59355.
SMRiO59355.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO59355.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 449ADPKUniRule annotationAdd BLAST449

Sequence similaritiesi

Belongs to the carbohydrate kinase PfkC family.UniRule annotation
Contains 1 ADPK (ADP-dependent kinase) domain.UniRule annotation

Phylogenomic databases

eggNOGiarCOG03370. Archaea.
COG4809. LUCA.
HOGENOMiHOG000254055.
KOiK00918.
OMAiHLEFASI.

Family and domain databases

Gene3Di3.40.1190.20. 1 hit.
HAMAPiMF_00561. ADP_PFKinase. 1 hit.
InterProiIPR007666. ADP_PFK/GK.
IPR015990. ADP_PFK/GK_arc.
IPR011790. ADP_PFK_arc.
IPR029056. Ribokinase-like.
[Graphical view]
PANTHERiPTHR21208. PTHR21208. 1 hit.
PfamiPF04587. ADP_PFK_GK. 1 hit.
[Graphical view]
PIRSFiPIRSF015883. ADP-Pfk_glckin. 1 hit.
SUPFAMiSSF53613. SSF53613. 1 hit.
TIGRFAMsiTIGR02045. P_fruct_ADP. 1 hit.
PROSITEiPS51255. ADPK. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O59355-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIPEHLSIYT AYNANIDAIV KLNQETIQNL INAFDPDEVK RRIEEYPREI
60 70 80 90 100
NEPIDFVARL VHTLKLGKPA AVPLVNEKMN EWFDKTFRYE EERLGGQAGI
110 120 130 140 150
IANTLAGLKI RKVIAYTPFL PKRLAELFKK GVLYPVVENG ELQFKPIQEA
160 170 180 190 200
YREGDPLKIN RIFEFRKGLK FKLGDETIEI PNSGRFIVSA RFESISRIET
210 220 230 240 250
REDIKPFLGE IGKEVDGAIF SGYQGLRTKY SDGKDANYYL RRAKEDIIEF
260 270 280 290 300
KEKDVKIHVE FASVQDRKLR KKIITNILPF VDSVGIDEAE IAQILSVLGY
310 320 330 340 350
RELADRIFTY NRLEDSILGG MIILDELNFE ILQVHTTYYL MYITHRDNPL
360 370 380 390 400
SEEELAKSLE FGTTLAAARA SLGDIRGPDD YKVGLKVPFN ERSEYVKLRF
410 420 430 440 450
EEAKSRLRMR EYKVVVIPTR LVQNPVLTVG LGDTISAGAF LTYLEFLKRH
Length:450
Mass (Da):51,753
Last modified:August 1, 1998 - v1
Checksum:iEC3B0836AA9314FE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000001 Genomic DNA. Translation: BAA30757.1.
PIRiE71044.
RefSeqiWP_010885713.1. NC_000961.1.

Genome annotation databases

EnsemblBacteriaiBAA30757; BAA30757; BAA30757.
GeneIDi1442494.
KEGGipho:PH1645.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000001 Genomic DNA. Translation: BAA30757.1.
PIRiE71044.
RefSeqiWP_010885713.1. NC_000961.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1U2XX-ray2.00A/B1-450[»]
3DRWX-ray1.90A/B1-450[»]
ProteinModelPortaliO59355.
SMRiO59355.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiO59355. 1 interactor.
MINTiMINT-1502590.
STRINGi70601.PH1645.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAA30757; BAA30757; BAA30757.
GeneIDi1442494.
KEGGipho:PH1645.

Phylogenomic databases

eggNOGiarCOG03370. Archaea.
COG4809. LUCA.
HOGENOMiHOG000254055.
KOiK00918.
OMAiHLEFASI.

Enzyme and pathway databases

UniPathwayiUPA00109.
BRENDAi2.7.1.146. 5244.

Miscellaneous databases

EvolutionaryTraceiO59355.

Family and domain databases

Gene3Di3.40.1190.20. 1 hit.
HAMAPiMF_00561. ADP_PFKinase. 1 hit.
InterProiIPR007666. ADP_PFK/GK.
IPR015990. ADP_PFK/GK_arc.
IPR011790. ADP_PFK_arc.
IPR029056. Ribokinase-like.
[Graphical view]
PANTHERiPTHR21208. PTHR21208. 1 hit.
PfamiPF04587. ADP_PFK_GK. 1 hit.
[Graphical view]
PIRSFiPIRSF015883. ADP-Pfk_glckin. 1 hit.
SUPFAMiSSF53613. SSF53613. 1 hit.
TIGRFAMsiTIGR02045. P_fruct_ADP. 1 hit.
PROSITEiPS51255. ADPK. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiK6PF_PYRHO
AccessioniPrimary (citable) accession number: O59355
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 6, 2002
Last sequence update: August 1, 1998
Last modified: November 2, 2016
This is version 106 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.