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Protein

ADP-specific phosphofructokinase

Gene

pfkC

Organism
Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the phosphorylation of fructose 6-phosphate to fructose 1,6-bisphosphate using ADP as the phosphate donor.UniRule annotation

Catalytic activityi

ADP + D-fructose 6-phosphate = AMP + D-fructose 1,6-bisphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 1 Mg2+ ion per subunit.UniRule annotation

Pathwayi: glycolysis

This protein is involved in the pathway glycolysis, which is part of Carbohydrate degradation.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi260 – 2601MagnesiumUniRule annotation
Metal bindingi290 – 2901MagnesiumUniRule annotation
Active sitei433 – 4331Proton acceptorUniRule annotation
Metal bindingi433 – 4331MagnesiumUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciPHOR70601:GJWR-1646-MONOMER.
BRENDAi2.7.1.146. 5244.
UniPathwayiUPA00109.

Names & Taxonomyi

Protein namesi
Recommended name:
ADP-specific phosphofructokinaseUniRule annotation (EC:2.7.1.146UniRule annotation)
Alternative name(s):
ADP-dependent phosphofructokinaseUniRule annotation
Short name:
ADP-PfkUniRule annotation
Gene namesi
Name:pfkCUniRule annotation
Ordered Locus Names:PH1645
OrganismiPyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Taxonomic identifieri70601 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
Proteomesi
  • UP000000752 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 450450ADP-specific phosphofructokinasePRO_0000184767Add
BLAST

Interactioni

Protein-protein interaction databases

IntActiO59355. 1 interaction.
MINTiMINT-1502590.
STRINGi70601.PH1645.

Structurei

Secondary structure

1
450
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi7 – 126Combined sources
Beta strandi15 – 217Combined sources
Helixi24 – 329Combined sources
Helixi36 – 4510Combined sources
Beta strandi48 – 525Combined sources
Helixi53 – 6614Combined sources
Beta strandi70 – 745Combined sources
Helixi79 – 868Combined sources
Beta strandi90 – 967Combined sources
Helixi97 – 10711Combined sources
Beta strandi111 – 1155Combined sources
Helixi122 – 1254Combined sources
Beta strandi132 – 14615Combined sources
Helixi147 – 1493Combined sources
Beta strandi159 – 1657Combined sources
Beta strandi170 – 1756Combined sources
Beta strandi177 – 1793Combined sources
Beta strandi184 – 1907Combined sources
Helixi194 – 1963Combined sources
Turni202 – 2043Combined sources
Helixi205 – 2073Combined sources
Helixi208 – 2147Combined sources
Beta strandi216 – 2205Combined sources
Helixi223 – 2253Combined sources
Helixi236 – 25217Combined sources
Beta strandi256 – 2605Combined sources
Helixi267 – 27610Combined sources
Helixi278 – 2803Combined sources
Beta strandi281 – 2877Combined sources
Helixi288 – 29811Combined sources
Helixi301 – 31010Combined sources
Helixi313 – 32715Combined sources
Beta strandi330 – 3356Combined sources
Beta strandi337 – 3459Combined sources
Helixi352 – 37221Combined sources
Helixi378 – 3803Combined sources
Helixi381 – 3855Combined sources
Helixi393 – 40614Combined sources
Beta strandi412 – 4187Combined sources
Helixi431 – 44717Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1U2XX-ray2.00A/B1-450[»]
3DRWX-ray1.90A/B1-450[»]
ProteinModelPortaliO59355.
SMRiO59355. Positions 1-450.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO59355.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 449449ADPKUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the carbohydrate kinase PfkC family.UniRule annotation
Contains 1 ADPK (ADP-dependent kinase) domain.UniRule annotation

Phylogenomic databases

eggNOGiarCOG03370. Archaea.
COG4809. LUCA.
HOGENOMiHOG000254055.
KOiK00918.
OMAiHLEFASI.

Family and domain databases

Gene3Di3.40.1190.20. 1 hit.
HAMAPiMF_00561. ADP_PFKinase.
InterProiIPR007666. ADP_PFK/GK.
IPR015990. ADP_PFK/GK_arc.
IPR011790. ADP_PFK_arc.
IPR029056. Ribokinase-like.
[Graphical view]
PANTHERiPTHR21208. PTHR21208. 1 hit.
PfamiPF04587. ADP_PFK_GK. 1 hit.
[Graphical view]
PIRSFiPIRSF015883. ADP-Pfk_glckin. 1 hit.
SUPFAMiSSF53613. SSF53613. 1 hit.
TIGRFAMsiTIGR02045. P_fruct_ADP. 1 hit.
PROSITEiPS51255. ADPK. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O59355-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIPEHLSIYT AYNANIDAIV KLNQETIQNL INAFDPDEVK RRIEEYPREI
60 70 80 90 100
NEPIDFVARL VHTLKLGKPA AVPLVNEKMN EWFDKTFRYE EERLGGQAGI
110 120 130 140 150
IANTLAGLKI RKVIAYTPFL PKRLAELFKK GVLYPVVENG ELQFKPIQEA
160 170 180 190 200
YREGDPLKIN RIFEFRKGLK FKLGDETIEI PNSGRFIVSA RFESISRIET
210 220 230 240 250
REDIKPFLGE IGKEVDGAIF SGYQGLRTKY SDGKDANYYL RRAKEDIIEF
260 270 280 290 300
KEKDVKIHVE FASVQDRKLR KKIITNILPF VDSVGIDEAE IAQILSVLGY
310 320 330 340 350
RELADRIFTY NRLEDSILGG MIILDELNFE ILQVHTTYYL MYITHRDNPL
360 370 380 390 400
SEEELAKSLE FGTTLAAARA SLGDIRGPDD YKVGLKVPFN ERSEYVKLRF
410 420 430 440 450
EEAKSRLRMR EYKVVVIPTR LVQNPVLTVG LGDTISAGAF LTYLEFLKRH
Length:450
Mass (Da):51,753
Last modified:August 1, 1998 - v1
Checksum:iEC3B0836AA9314FE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000001 Genomic DNA. Translation: BAA30757.1.
PIRiE71044.
RefSeqiWP_010885713.1. NC_000961.1.

Genome annotation databases

EnsemblBacteriaiBAA30757; BAA30757; BAA30757.
GeneIDi1442494.
KEGGipho:PH1645.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000001 Genomic DNA. Translation: BAA30757.1.
PIRiE71044.
RefSeqiWP_010885713.1. NC_000961.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1U2XX-ray2.00A/B1-450[»]
3DRWX-ray1.90A/B1-450[»]
ProteinModelPortaliO59355.
SMRiO59355. Positions 1-450.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiO59355. 1 interaction.
MINTiMINT-1502590.
STRINGi70601.PH1645.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAA30757; BAA30757; BAA30757.
GeneIDi1442494.
KEGGipho:PH1645.

Phylogenomic databases

eggNOGiarCOG03370. Archaea.
COG4809. LUCA.
HOGENOMiHOG000254055.
KOiK00918.
OMAiHLEFASI.

Enzyme and pathway databases

UniPathwayiUPA00109.
BioCyciPHOR70601:GJWR-1646-MONOMER.
BRENDAi2.7.1.146. 5244.

Miscellaneous databases

EvolutionaryTraceiO59355.

Family and domain databases

Gene3Di3.40.1190.20. 1 hit.
HAMAPiMF_00561. ADP_PFKinase.
InterProiIPR007666. ADP_PFK/GK.
IPR015990. ADP_PFK/GK_arc.
IPR011790. ADP_PFK_arc.
IPR029056. Ribokinase-like.
[Graphical view]
PANTHERiPTHR21208. PTHR21208. 1 hit.
PfamiPF04587. ADP_PFK_GK. 1 hit.
[Graphical view]
PIRSFiPIRSF015883. ADP-Pfk_glckin. 1 hit.
SUPFAMiSSF53613. SSF53613. 1 hit.
TIGRFAMsiTIGR02045. P_fruct_ADP. 1 hit.
PROSITEiPS51255. ADPK. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3.

Entry informationi

Entry nameiK6PF_PYRHO
AccessioniPrimary (citable) accession number: O59355
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 6, 2002
Last sequence update: August 1, 1998
Last modified: November 11, 2015
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.