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Protein

Ribonuclease HII

Gene

rnhB

Organism
Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Endonuclease that specifically degrades the RNA of RNA-DNA hybrids.By similarity

Catalytic activityi

Endonucleolytic cleavage to 5'-phosphomonoester.

Cofactori

Mn2+By similarity, Mg2+By similarityNote: Manganese or magnesium. Binds 1 divalent metal ion per monomer in the absence of substrate. May bind a second metal ion after substrate binding.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi7Divalent metal cationBy similarity1
Metal bindingi8Divalent metal cationBy similarity1
Metal bindingi105Divalent metal cationBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionEndonuclease, Hydrolase, Nuclease
LigandManganese, Metal-binding

Enzyme and pathway databases

BioCyciPHOR70601:G1G39-1600-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonuclease HII (EC:3.1.26.4)
Short name:
RNase HII
Gene namesi
Name:rnhB
Ordered Locus Names:PH1650
OrganismiPyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Taxonomic identifieri70601 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
Proteomesi
  • UP000000752 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001116701 – 220Ribonuclease HIIAdd BLAST220

Interactioni

Protein-protein interaction databases

STRINGi70601.PH1650

Structurei

Secondary structure

1220
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi2 – 9Combined sources8
Beta strandi14 – 16Combined sources3
Beta strandi18 – 26Combined sources9
Helixi27 – 29Combined sources3
Helixi30 – 36Combined sources7
Helixi38 – 40Combined sources3
Helixi46 – 59Combined sources14
Beta strandi60 – 68Combined sources9
Helixi70 – 74Combined sources5
Helixi80 – 93Combined sources14
Beta strandi100 – 105Combined sources6
Helixi111 – 121Combined sources11
Beta strandi127 – 131Combined sources5
Helixi134 – 137Combined sources4
Helixi139 – 163Combined sources25
Helixi174 – 187Combined sources14
Helixi199 – 208Combined sources10

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1UAXX-ray2.00A/B1-220[»]
ProteinModelPortaliO59351
SMRiO59351
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO59351

Family & Domainsi

Sequence similaritiesi

Belongs to the RNase HII family.Curated

Phylogenomic databases

eggNOGiarCOG04121 Archaea
COG0164 LUCA
HOGENOMiHOG000100289
KOiK03470
OMAiREECRFF
OrthoDBiPOG093Z0CAA

Family and domain databases

Gene3Di1.10.10.460, 1 hit
3.30.420.10, 1 hit
HAMAPiMF_00052_A RNase_HII_A, 1 hit
InterProiView protein in InterPro
IPR004649 RNase_H2_suA
IPR001352 RNase_HII/HIII
IPR024567 RNase_HII/HIII_dom
IPR020787 RNase_HII_arc
IPR023160 RNase_HII_hlx-loop-hlx_cap_dom
IPR012337 RNaseH-like_sf
IPR036397 RNaseH_sf
PANTHERiPTHR10954 PTHR10954, 1 hit
PfamiView protein in Pfam
PF01351 RNase_HII, 1 hit
SUPFAMiSSF53098 SSF53098, 1 hit
TIGRFAMsiTIGR00729 TIGR00729, 1 hit

Sequencei

Sequence statusi: Complete.

O59351-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKVAGVDEAG RGPVIGPLVI GVAVIDEKNI ERLRDIGVKD SKQLTPGQRE
60 70 80 90 100
KLFSKLIDIL DDYYVLLVTP KEIDERHHSM NELEAEKFVV ALNSLRIKPQ
110 120 130 140 150
KIYVDSADVD PKRFASLIKA GLKYEATVIA EHKADAKYEI VSAASIIAKV
160 170 180 190 200
TRDREIEKLK QKYGEFGSGY PSDPRTKEWL EEYYKQYGDF PPIVRRTWET
210 220
ARKIEERFRK NQLTLDKFLK
Length:220
Mass (Da):25,283
Last modified:August 1, 1998 - v1
Checksum:i77DB1BDEDB8881D0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000001 Genomic DNA Translation: BAA30762.1
PIRiB71045
RefSeqiWP_010885718.1, NC_000961.1

Genome annotation databases

EnsemblBacteriaiBAA30762; BAA30762; BAA30762
GeneIDi1442499
KEGGipho:PH1650

Similar proteinsi

Entry informationi

Entry nameiRNH2_PYRHO
AccessioniPrimary (citable) accession number: O59351
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: August 1, 1998
Last modified: April 25, 2018
This is version 112 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
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Main funding by: National Institutes of Health