Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Ribonuclease HII

Gene

rnhB

Organism
Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Endonuclease that specifically degrades the RNA of RNA-DNA hybrids.By similarity

Catalytic activityi

Endonucleolytic cleavage to 5'-phosphomonoester.

Cofactori

Mn2+By similarity, Mg2+By similarityNote: Manganese or magnesium. Binds 1 divalent metal ion per monomer in the absence of substrate. May bind a second metal ion after substrate binding.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi7 – 71Divalent metal cationBy similarity
Metal bindingi8 – 81Divalent metal cationBy similarity
Metal bindingi105 – 1051Divalent metal cationBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Ligandi

Manganese, Metal-binding

Enzyme and pathway databases

BioCyciPHOR70601:GJWR-1651-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonuclease HII (EC:3.1.26.4)
Short name:
RNase HII
Gene namesi
Name:rnhB
Ordered Locus Names:PH1650
OrganismiPyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Taxonomic identifieri70601 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
Proteomesi
  • UP000000752 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 220220Ribonuclease HIIPRO_0000111670Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi70601.PH1650.

Structurei

Secondary structure

1
220
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi2 – 98Combined sources
Beta strandi14 – 163Combined sources
Beta strandi18 – 269Combined sources
Helixi27 – 293Combined sources
Helixi30 – 367Combined sources
Helixi38 – 403Combined sources
Helixi46 – 5914Combined sources
Beta strandi60 – 689Combined sources
Helixi70 – 745Combined sources
Helixi80 – 9314Combined sources
Beta strandi100 – 1056Combined sources
Helixi111 – 12111Combined sources
Beta strandi127 – 1315Combined sources
Helixi134 – 1374Combined sources
Helixi139 – 16325Combined sources
Helixi174 – 18714Combined sources
Helixi199 – 20810Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1UAXX-ray2.00A/B1-220[»]
ProteinModelPortaliO59351.
SMRiO59351. Positions 1-211.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO59351.

Family & Domainsi

Sequence similaritiesi

Belongs to the RNase HII family.Curated

Phylogenomic databases

eggNOGiarCOG04121. Archaea.
COG0164. LUCA.
HOGENOMiHOG000100289.
KOiK03470.
OMAiAYVDACD.

Family and domain databases

Gene3Di1.10.10.460. 1 hit.
3.30.420.10. 1 hit.
HAMAPiMF_00052_A. RNase_HII_A.
InterProiIPR004649. RNase_H2_suA.
IPR001352. RNase_HII/HIII.
IPR024567. RNase_HII/HIII_dom.
IPR020787. RNase_HII_arc.
IPR023160. RNase_HII_hlx-loop-hlx_cap_dom.
IPR012337. RNaseH-like_dom.
[Graphical view]
PANTHERiPTHR10954. PTHR10954. 1 hit.
PfamiPF01351. RNase_HII. 1 hit.
[Graphical view]
SUPFAMiSSF53098. SSF53098. 1 hit.
TIGRFAMsiTIGR00729. TIGR00729. 1 hit.

Sequencei

Sequence statusi: Complete.

O59351-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKVAGVDEAG RGPVIGPLVI GVAVIDEKNI ERLRDIGVKD SKQLTPGQRE
60 70 80 90 100
KLFSKLIDIL DDYYVLLVTP KEIDERHHSM NELEAEKFVV ALNSLRIKPQ
110 120 130 140 150
KIYVDSADVD PKRFASLIKA GLKYEATVIA EHKADAKYEI VSAASIIAKV
160 170 180 190 200
TRDREIEKLK QKYGEFGSGY PSDPRTKEWL EEYYKQYGDF PPIVRRTWET
210 220
ARKIEERFRK NQLTLDKFLK
Length:220
Mass (Da):25,283
Last modified:August 1, 1998 - v1
Checksum:i77DB1BDEDB8881D0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000001 Genomic DNA. Translation: BAA30762.1.
PIRiB71045.
RefSeqiWP_010885718.1. NC_000961.1.

Genome annotation databases

EnsemblBacteriaiBAA30762; BAA30762; BAA30762.
GeneIDi1442499.
KEGGipho:PH1650.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000001 Genomic DNA. Translation: BAA30762.1.
PIRiB71045.
RefSeqiWP_010885718.1. NC_000961.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1UAXX-ray2.00A/B1-220[»]
ProteinModelPortaliO59351.
SMRiO59351. Positions 1-211.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi70601.PH1650.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAA30762; BAA30762; BAA30762.
GeneIDi1442499.
KEGGipho:PH1650.

Phylogenomic databases

eggNOGiarCOG04121. Archaea.
COG0164. LUCA.
HOGENOMiHOG000100289.
KOiK03470.
OMAiAYVDACD.

Enzyme and pathway databases

BioCyciPHOR70601:GJWR-1651-MONOMER.

Miscellaneous databases

EvolutionaryTraceiO59351.

Family and domain databases

Gene3Di1.10.10.460. 1 hit.
3.30.420.10. 1 hit.
HAMAPiMF_00052_A. RNase_HII_A.
InterProiIPR004649. RNase_H2_suA.
IPR001352. RNase_HII/HIII.
IPR024567. RNase_HII/HIII_dom.
IPR020787. RNase_HII_arc.
IPR023160. RNase_HII_hlx-loop-hlx_cap_dom.
IPR012337. RNaseH-like_dom.
[Graphical view]
PANTHERiPTHR10954. PTHR10954. 1 hit.
PfamiPF01351. RNase_HII. 1 hit.
[Graphical view]
SUPFAMiSSF53098. SSF53098. 1 hit.
TIGRFAMsiTIGR00729. TIGR00729. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3.

Entry informationi

Entry nameiRNH2_PYRHO
AccessioniPrimary (citable) accession number: O59351
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: August 1, 1998
Last modified: November 11, 2015
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.