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O59314 (SYE_PYRHO) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 88. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glutamate--tRNA ligase

EC=6.1.1.17
Alternative name(s):
Glutamyl-tRNA synthetase
Short name=GluRS
Gene names
Name:gltX
Ordered Locus Names:PH1686
OrganismPyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3) [Complete proteome] [HAMAP]
Taxonomic identifier70601 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus

Protein attributes

Sequence length570 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity. HAMAP-Rule MF_00022

Catalytic activity

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu). HAMAP-Rule MF_00022

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00022.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processglutamyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

glutamate-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 570570Glutamate--tRNA ligase HAMAP-Rule MF_00022
PRO_0000119728

Regions

Motif105 – 11511"HIGH" region HAMAP-Rule MF_00022

Sequences

Sequence LengthMass (Da)Tools
O59314 [UniParc].

Last modified August 1, 1998. Version 1.
Checksum: 51ED48F94891B963

FASTA57066,125
        10         20         30         40         50         60 
MDVEKIALKH ALINAIEHGG KANLKAVIGK VLGENPELRP RAKEIIPIIN KVVEEVNSLA 

        70         80         90        100        110        120 
RDEQLEKLKD IYPEYFEKKE EKKEKKGLPL LPKAEKGKVV TRFAPNPDGA FHLGNARAAI 

       130        140        150        160        170        180 
LSYEYAKMYG GKFILRFDDT DPKVKRPEPI FYKMIIEDLE WLGIKPDEIV YASDRLEIYY 

       190        200        210        220        230        240 
KYAEELIKMG KAYVCTCPPE KFRELRDKGI PCPHRDEPVE VQLERWKKML NGEYKEGEAV 

       250        260        270        280        290        300 
VRIKTDLNHP NPAVRDWPAL RIIDNPNHPR TGNKYRVWPL YNFASAIDDH ELGVTHIFRG 

       310        320        330        340        350        360 
QEHAENETRQ RYIYEYFGWE YPVTIHHGRL SIEGVVLSKS KTRKGIEEGK YLGWDDPRLG 

       370        380        390        400        410        420 
TIRALRRRGI LPEAIKELII EVGLKKSDAT ISWENLAAIN RKLVDPIANR YFFVADPIPM 

       430        440        450        460        470        480 
EVEGAPEFIA EIPLHPDHPE RGVRRLKFTP ERPVYVSKDD LNLLKPGNFV RLKDLFNVEI 

       490        500        510        520        530        540 
LEVGDKIRAR FYSFEYEIAK KNRWKMVHWV TEGRPCEVII PEGDELVVRK GLLEKDAKVQ 

       550        560        570 
VNEIVQFERF GFVRIDRIEG DKVIAIYAHK 

« Hide

References

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BA000001 Genomic DNA. Translation: BAA30798.1.
PIRF71049.
RefSeqNP_143530.1. NC_000961.1.

3D structure databases

ProteinModelPortalO59314.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING70601.PH1686.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAA30798; BAA30798; BAA30798.
GeneID1442532.
KEGGpho:PH1686.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0008.
HOGENOMHOG000073585.
KOK01885.
OMAMRFAPNP.
ProtClustDBPRK04156.

Enzyme and pathway databases

BioCycPHOR70601:GJWR-1684-MONOMER.

Family and domain databases

Gene3D1.10.1160.10. 1 hit.
2.40.240.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPMF_00022_A. Glu_tRNA_synth_A.
InterProIPR004526. Glu-tRNA-synth_arc/euk.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR020059. Glu/Gln-tRNA-synth_Ib_codon-bd.
IPR022888. Glu_tRNA_synth_arc.
IPR020056. Rbsml_L25/Gln-tRNA_synth_b-brl.
IPR011035. Ribosomal_L25/Gln-tRNA_synth.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERPTHR10119. PTHR10119. 1 hit.
PfamPF00749. tRNA-synt_1c. 1 hit.
PF03950. tRNA-synt_1c_C. 1 hit.
[Graphical view]
PRINTSPR00987. TRNASYNTHGLU.
SUPFAMSSF50715. SSF50715. 1 hit.
TIGRFAMsTIGR00463. gltX_arch. 1 hit.
ProtoNetSearch...

Entry information

Entry nameSYE_PYRHO
AccessionPrimary (citable) accession number: O59314
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: August 1, 1998
Last modified: February 19, 2014
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries