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Protein

Glutamate--tRNA ligase

Gene

gltX

Organism
Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).UniRule annotation

Catalytic activityi

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).UniRule annotation

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciPHOR70601:G1G39-1632-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate--tRNA ligaseUniRule annotation (EC:6.1.1.17UniRule annotation)
Alternative name(s):
Glutamyl-tRNA synthetaseUniRule annotation
Short name:
GluRSUniRule annotation
Gene namesi
Name:gltXUniRule annotation
Ordered Locus Names:PH1686
OrganismiPyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Taxonomic identifieri70601 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
Proteomesi
  • UP000000752 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001197281 – 570Glutamate--tRNA ligaseAdd BLAST570

Interactioni

Protein-protein interaction databases

STRINGi70601.PH1686

Structurei

3D structure databases

ProteinModelPortaliO59314
SMRiO59314
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi105 – 115"HIGH" regionUniRule annotationAdd BLAST11

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. Glutamate--tRNA ligase type 2 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiarCOG04302 Archaea
COG0008 LUCA
HOGENOMiHOG000073585
KOiK01885
OMAiMRFAPNP
OrthoDBiPOG093Z013J

Family and domain databases

Gene3Di2.40.240.10, 1 hit
3.40.50.620, 1 hit
HAMAPiMF_02076 Glu_tRNA_synth_type2, 1 hit
InterProiView protein in InterPro
IPR004526 Glu-tRNA-synth_arc/euk
IPR000924 Glu/Gln-tRNA-synth
IPR020058 Glu/Gln-tRNA-synth_Ib_cat-dom
IPR020059 Glu/Gln-tRNA-synth_Ib_codon-bd
IPR020056 Rbsml_L25/Gln-tRNA_synth_N
IPR011035 Ribosomal_L25/Gln-tRNA_synth
IPR014729 Rossmann-like_a/b/a_fold
PfamiView protein in Pfam
PF00749 tRNA-synt_1c, 1 hit
PF03950 tRNA-synt_1c_C, 1 hit
PRINTSiPR00987 TRNASYNTHGLU
SUPFAMiSSF50715 SSF50715, 1 hit
TIGRFAMsiTIGR00463 gltX_arch, 1 hit

Sequencei

Sequence statusi: Complete.

O59314-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDVEKIALKH ALINAIEHGG KANLKAVIGK VLGENPELRP RAKEIIPIIN
60 70 80 90 100
KVVEEVNSLA RDEQLEKLKD IYPEYFEKKE EKKEKKGLPL LPKAEKGKVV
110 120 130 140 150
TRFAPNPDGA FHLGNARAAI LSYEYAKMYG GKFILRFDDT DPKVKRPEPI
160 170 180 190 200
FYKMIIEDLE WLGIKPDEIV YASDRLEIYY KYAEELIKMG KAYVCTCPPE
210 220 230 240 250
KFRELRDKGI PCPHRDEPVE VQLERWKKML NGEYKEGEAV VRIKTDLNHP
260 270 280 290 300
NPAVRDWPAL RIIDNPNHPR TGNKYRVWPL YNFASAIDDH ELGVTHIFRG
310 320 330 340 350
QEHAENETRQ RYIYEYFGWE YPVTIHHGRL SIEGVVLSKS KTRKGIEEGK
360 370 380 390 400
YLGWDDPRLG TIRALRRRGI LPEAIKELII EVGLKKSDAT ISWENLAAIN
410 420 430 440 450
RKLVDPIANR YFFVADPIPM EVEGAPEFIA EIPLHPDHPE RGVRRLKFTP
460 470 480 490 500
ERPVYVSKDD LNLLKPGNFV RLKDLFNVEI LEVGDKIRAR FYSFEYEIAK
510 520 530 540 550
KNRWKMVHWV TEGRPCEVII PEGDELVVRK GLLEKDAKVQ VNEIVQFERF
560 570
GFVRIDRIEG DKVIAIYAHK
Length:570
Mass (Da):66,125
Last modified:August 1, 1998 - v1
Checksum:i51ED48F94891B963
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000001 Genomic DNA Translation: BAA30798.1
PIRiF71049
RefSeqiWP_010885751.1, NC_000961.1

Genome annotation databases

EnsemblBacteriaiBAA30798; BAA30798; BAA30798
GeneIDi1442532
KEGGipho:PH1686

Similar proteinsi

Entry informationi

Entry nameiSYE_PYRHO
AccessioniPrimary (citable) accession number: O59314
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: August 1, 1998
Last modified: April 25, 2018
This is version 108 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families
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