ID   DNLI_PYRHO              Reviewed;         559 AA.
AC   O59288;
DT   30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1998, sequence version 1.
DT   16-JUN-2009, entry version 63.
DE   RecName: Full=Probable thermostable DNA ligase;
DE            EC=6.5.1.1;
DE   AltName: Full=Polydeoxyribonucleotide synthase [ATP];
GN   Name=lig; OrderedLocusNames=PH1622;
OS   Pyrococcus horikoshii.
OC   Archaea; Euryarchaeota; Thermococci; Thermococcales; Thermococcaceae;
OC   Pyrococcus.
OX   NCBI_TaxID=53953;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=OT3;
RX   MEDLINE=98344137; PubMed=9679194; DOI=10.1093/dnares/5.2.55;
RA   Kawarabayasi Y., Sawada M., Horikawa H., Haikawa Y., Hino Y.,
RA   Yamamoto S., Sekine M., Baba S., Kosugi H., Hosoyama A., Nagai Y.,
RA   Sakai M., Ogura K., Otsuka R., Nakazawa H., Takamiya M., Ohfuku Y.,
RA   Funahashi T., Tanaka T., Kudoh Y., Yamazaki J., Kushida N., Oguchi A.,
RA   Aoki K., Yoshizawa T., Nakamura Y., Robb F.T., Horikoshi K.,
RA   Masuchi Y., Shizuya H., Kikuchi H.;
RT   "Complete sequence and gene organization of the genome of a hyper-
RT   thermophilic archaebacterium, Pyrococcus horikoshii OT3.";
RL   DNA Res. 5:55-76(1998).
CC   -!- FUNCTION: DNA ligase that seals nicks in double-stranded DNA
CC       during DNA replication, DNA recombination and DNA repair (By
CC       similarity).
CC   -!- CATALYTIC ACTIVITY: ATP + (deoxyribonucleotide)(n) +
CC       (deoxyribonucleotide)(m) = AMP + diphosphate +
CC       (deoxyribonucleotide)(n+m).
CC   -!- COFACTOR: Magnesium (By similarity).
CC   -!- SUBUNIT: Monomer (By similarity).
CC   -!- SIMILARITY: Belongs to the ATP-dependent DNA ligase family.
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DR   EMBL; BA000001; BAA30734.1; -; Genomic_DNA.
DR   PIR; F71041; F71041.
DR   RefSeq; NP_143476.1; -.
DR   SMR; O59288; 1-559.
DR   GeneID; 1442475; -.
DR   GenomeReviews; BA000001_GR; PH1622.
DR   KEGG; pho:PH1622; -.
DR   NMPDR; fig|70601.1.peg.1589; -.
DR   HOGENOM; O59288; -.
DR   OMA; O59288; SNLHLGA.
DR   BRENDA; 6.5.1.1; 74679.
DR   GO; GO:0005524; F:ATP binding; IEA:HAMAP.
DR   GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR   GO; GO:0003910; F:DNA ligase (ATP) activity; IEA:HAMAP.
DR   GO; GO:0000287; F:magnesium ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
DR   GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR   GO; GO:0006310; P:DNA recombination; IEA:HAMAP.
DR   GO; GO:0006281; P:DNA repair; IEA:HAMAP.
DR   GO; GO:0006260; P:DNA replication; IEA:HAMAP.
DR   HAMAP; MF_00407; -; 1.
DR   InterPro; IPR000977; DNA_ligase.
DR   InterPro; IPR012309; DNA_ligase_A_C.
DR   InterPro; IPR012310; DNA_ligase_A_M.
DR   InterPro; IPR012308; DNA_ligase_A_N.
DR   InterPro; IPR016059; DNA_ligase_CS.
DR   InterPro; IPR012340; NA-bd_OB-fold.
DR   Gene3D; G3DSA:2.40.50.140; OB_NA_bd_sub; 1.
DR   Pfam; PF04679; DNA_ligase_A_C; 1.
DR   Pfam; PF01068; DNA_ligase_A_M; 1.
DR   Pfam; PF04675; DNA_ligase_A_N; 1.
DR   TIGRFAMs; TIGR00574; dnl1; 1.
DR   PROSITE; PS00697; DNA_LIGASE_A1; 1.
DR   PROSITE; PS00333; DNA_LIGASE_A2; 1.
DR   PROSITE; PS50160; DNA_LIGASE_A3; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Cell cycle; Cell division; Complete proteome; DNA damage;
KW   DNA recombination; DNA repair; DNA replication; Ligase; Magnesium;
KW   Metal-binding; Nucleotide-binding.
FT   CHAIN         1    559       Probable thermostable DNA ligase.
FT                                /FTId=PRO_0000059614.
FT   ACT_SITE    249    249       N6-AMP-lysine intermediate (By
FT                                similarity).
FT   BINDING     247    247       ATP (By similarity).
FT   BINDING     254    254       ATP (By similarity).
FT   BINDING     269    269       ATP (By similarity).
FT   BINDING     299    299       ATP (By similarity).
FT   BINDING     339    339       ATP (By similarity).
FT   BINDING     414    414       ATP (By similarity).
FT   BINDING     420    420       ATP (By similarity).
SQ   SEQUENCE   559 AA;  63623 MW;  D1E85172B1D72188 CRC64;
     MKYIELAQLY QKLEKTTMKL IKTRLVADFL KRVPEDHLDF IPYLILGDVF PEWDERELGV
     GEKLLIKAVS MATGVDPKEI ENSVKDTGDL GESIALAIKR RKQKSFFSQP LTIKRVYQTL
     VKIAETTGEG SQEKKMKYLA NLFMDAEPLE AKYLARTVLG TMRTGVAEGL LRDAIALAFH
     VKVELVERAY MLTSDFGFVA KIAKAEGNEG LEKVTIQLGK PIKPMLAQQA ANIKEALLEM
     GGEAEFEIKY DGARVQVHKD GEKVIIYSRR LENVTRAIPE IVEAIKEAIE PEKVIVEGEL
     VAIGEEGRPL PFQYVLRRFR RKHNIEEMMK KIPLELNLFD VLYVDGKSFV DTKFIERRKK
     LEEIVKPNGK IKVAENLITK NAEEAEKFYK RALEMGHEGL MAKRLDAVYE PGNRGKKWLK
     IKPTMENLDL VIIGAEWGEG RRAHLFGSFI LGAYDPETGE FLEVGKVGSG FTDDDLVEFT
     KMLKPLIVKE EGKRVVIQPK IVIEVTYQEI QKSPKYRSGF ALRFPRYVAL RDDKGPEDAD
     TIERIAQLYE LQERIKGKV
//