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O59288

- DNLI_PYRHO

UniProt

O59288 - DNLI_PYRHO

Protein

Probable thermostable DNA ligase

Gene

lig

Organism
Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 99 (01 Oct 2014)
      Sequence version 1 (01 Aug 1998)
      Previous versions | rss
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    Functioni

    DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair.By similarity

    Catalytic activityi

    ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m).

    Cofactori

    Magnesium.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei247 – 2471ATPBy similarity
    Active sitei249 – 2491N6-AMP-lysine intermediateBy similarity
    Binding sitei254 – 2541ATPBy similarity
    Binding sitei269 – 2691ATPBy similarity
    Binding sitei299 – 2991ATPBy similarity
    Binding sitei339 – 3391ATPBy similarity
    Binding sitei414 – 4141ATPBy similarity
    Binding sitei420 – 4201ATPBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-HAMAP
    2. DNA binding Source: InterPro
    3. DNA ligase (ATP) activity Source: UniProtKB-HAMAP
    4. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. cell cycle Source: UniProtKB-KW
    2. cell division Source: UniProtKB-KW
    3. DNA ligation involved in DNA repair Source: InterPro
    4. DNA recombination Source: UniProtKB-HAMAP
    5. DNA replication Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    Cell cycle, Cell division, DNA damage, DNA recombination, DNA repair, DNA replication

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciPHOR70601:GJWR-1624-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Probable thermostable DNA ligase (EC:6.5.1.1)
    Alternative name(s):
    Polydeoxyribonucleotide synthase [ATP]
    Gene namesi
    Name:lig
    Ordered Locus Names:PH1622
    OrganismiPyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
    Taxonomic identifieri70601 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
    ProteomesiUP000000752: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 559559Probable thermostable DNA ligasePRO_0000059614Add
    BLAST

    Proteomic databases

    PRIDEiO59288.

    Interactioni

    Subunit structurei

    Monomer.By similarity

    Protein-protein interaction databases

    STRINGi70601.PH1622.

    Structurei

    3D structure databases

    ProteinModelPortaliO59288.
    SMRiO59288. Positions 1-559.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the ATP-dependent DNA ligase family.Curated

    Phylogenomic databases

    eggNOGiCOG1793.
    HOGENOMiHOG000036008.
    KOiK10747.
    OMAiARVQVHK.

    Family and domain databases

    Gene3Di1.10.3260.10. 1 hit.
    2.40.50.140. 1 hit.
    HAMAPiMF_00407. DNA_ligase.
    InterProiIPR022865. DNA_ligae_ATP-dep_bac/arc.
    IPR000977. DNA_ligase_ATP-dep.
    IPR012309. DNA_ligase_ATP-dep_C.
    IPR012310. DNA_ligase_ATP-dep_cent.
    IPR016059. DNA_ligase_ATP-dep_CS.
    IPR012308. DNA_ligase_ATP-dep_N.
    IPR012340. NA-bd_OB-fold.
    [Graphical view]
    PfamiPF04679. DNA_ligase_A_C. 1 hit.
    PF01068. DNA_ligase_A_M. 1 hit.
    PF04675. DNA_ligase_A_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF117018. SSF117018. 1 hit.
    SSF50249. SSF50249. 1 hit.
    TIGRFAMsiTIGR00574. dnl1. 1 hit.
    PROSITEiPS00697. DNA_LIGASE_A1. 1 hit.
    PS00333. DNA_LIGASE_A2. 1 hit.
    PS50160. DNA_LIGASE_A3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O59288-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKYIELAQLY QKLEKTTMKL IKTRLVADFL KRVPEDHLDF IPYLILGDVF    50
    PEWDERELGV GEKLLIKAVS MATGVDPKEI ENSVKDTGDL GESIALAIKR 100
    RKQKSFFSQP LTIKRVYQTL VKIAETTGEG SQEKKMKYLA NLFMDAEPLE 150
    AKYLARTVLG TMRTGVAEGL LRDAIALAFH VKVELVERAY MLTSDFGFVA 200
    KIAKAEGNEG LEKVTIQLGK PIKPMLAQQA ANIKEALLEM GGEAEFEIKY 250
    DGARVQVHKD GEKVIIYSRR LENVTRAIPE IVEAIKEAIE PEKVIVEGEL 300
    VAIGEEGRPL PFQYVLRRFR RKHNIEEMMK KIPLELNLFD VLYVDGKSFV 350
    DTKFIERRKK LEEIVKPNGK IKVAENLITK NAEEAEKFYK RALEMGHEGL 400
    MAKRLDAVYE PGNRGKKWLK IKPTMENLDL VIIGAEWGEG RRAHLFGSFI 450
    LGAYDPETGE FLEVGKVGSG FTDDDLVEFT KMLKPLIVKE EGKRVVIQPK 500
    IVIEVTYQEI QKSPKYRSGF ALRFPRYVAL RDDKGPEDAD TIERIAQLYE 550
    LQERIKGKV 559
    Length:559
    Mass (Da):63,623
    Last modified:August 1, 1998 - v1
    Checksum:iD1E85172B1D72188
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000001 Genomic DNA. Translation: BAA30734.1.
    PIRiF71041.
    RefSeqiNP_143476.1. NC_000961.1.
    WP_010885696.1. NC_000961.1.

    Genome annotation databases

    EnsemblBacteriaiBAA30734; BAA30734; BAA30734.
    GeneIDi1442475.
    KEGGipho:PH1622.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000001 Genomic DNA. Translation: BAA30734.1 .
    PIRi F71041.
    RefSeqi NP_143476.1. NC_000961.1.
    WP_010885696.1. NC_000961.1.

    3D structure databases

    ProteinModelPortali O59288.
    SMRi O59288. Positions 1-559.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 70601.PH1622.

    Proteomic databases

    PRIDEi O59288.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAA30734 ; BAA30734 ; BAA30734 .
    GeneIDi 1442475.
    KEGGi pho:PH1622.

    Phylogenomic databases

    eggNOGi COG1793.
    HOGENOMi HOG000036008.
    KOi K10747.
    OMAi ARVQVHK.

    Enzyme and pathway databases

    BioCyci PHOR70601:GJWR-1624-MONOMER.

    Family and domain databases

    Gene3Di 1.10.3260.10. 1 hit.
    2.40.50.140. 1 hit.
    HAMAPi MF_00407. DNA_ligase.
    InterProi IPR022865. DNA_ligae_ATP-dep_bac/arc.
    IPR000977. DNA_ligase_ATP-dep.
    IPR012309. DNA_ligase_ATP-dep_C.
    IPR012310. DNA_ligase_ATP-dep_cent.
    IPR016059. DNA_ligase_ATP-dep_CS.
    IPR012308. DNA_ligase_ATP-dep_N.
    IPR012340. NA-bd_OB-fold.
    [Graphical view ]
    Pfami PF04679. DNA_ligase_A_C. 1 hit.
    PF01068. DNA_ligase_A_M. 1 hit.
    PF04675. DNA_ligase_A_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF117018. SSF117018. 1 hit.
    SSF50249. SSF50249. 1 hit.
    TIGRFAMsi TIGR00574. dnl1. 1 hit.
    PROSITEi PS00697. DNA_LIGASE_A1. 1 hit.
    PS00333. DNA_LIGASE_A2. 1 hit.
    PS50160. DNA_LIGASE_A3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3.

    Entry informationi

    Entry nameiDNLI_PYRHO
    AccessioniPrimary (citable) accession number: O59288
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 30, 2000
    Last sequence update: August 1, 1998
    Last modified: October 1, 2014
    This is version 99 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3