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O59288

- DNLI_PYRHO

UniProt

O59288 - DNLI_PYRHO

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Protein

Probable thermostable DNA ligase

Gene

lig

Organism
Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair.By similarity

Catalytic activityi

ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m).

Cofactori

Magnesium.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei247 – 2471ATPBy similarity
Active sitei249 – 2491N6-AMP-lysine intermediateBy similarity
Binding sitei254 – 2541ATPBy similarity
Binding sitei269 – 2691ATPBy similarity
Binding sitei299 – 2991ATPBy similarity
Binding sitei339 – 3391ATPBy similarity
Binding sitei414 – 4141ATPBy similarity
Binding sitei420 – 4201ATPBy similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. DNA binding Source: InterPro
  3. DNA ligase (ATP) activity Source: UniProtKB-HAMAP
  4. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. cell cycle Source: UniProtKB-KW
  2. cell division Source: UniProtKB-KW
  3. DNA biosynthetic process Source: InterPro
  4. DNA ligation involved in DNA repair Source: InterPro
  5. DNA recombination Source: UniProtKB-HAMAP
  6. DNA replication Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Cell cycle, Cell division, DNA damage, DNA recombination, DNA repair, DNA replication

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciPHOR70601:GJWR-1624-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable thermostable DNA ligase (EC:6.5.1.1)
Alternative name(s):
Polydeoxyribonucleotide synthase [ATP]
Gene namesi
Name:lig
Ordered Locus Names:PH1622
OrganismiPyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Taxonomic identifieri70601 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
ProteomesiUP000000752: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 559559Probable thermostable DNA ligasePRO_0000059614Add
BLAST

Proteomic databases

PRIDEiO59288.

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

STRINGi70601.PH1622.

Structurei

3D structure databases

ProteinModelPortaliO59288.
SMRiO59288. Positions 1-559.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ATP-dependent DNA ligase family.Curated

Phylogenomic databases

eggNOGiCOG1793.
HOGENOMiHOG000036008.
KOiK10747.
OMAiARVQVHK.

Family and domain databases

Gene3Di1.10.3260.10. 1 hit.
2.40.50.140. 1 hit.
HAMAPiMF_00407. DNA_ligase.
InterProiIPR022865. DNA_ligae_ATP-dep_bac/arc.
IPR000977. DNA_ligase_ATP-dep.
IPR012309. DNA_ligase_ATP-dep_C.
IPR012310. DNA_ligase_ATP-dep_cent.
IPR016059. DNA_ligase_ATP-dep_CS.
IPR012308. DNA_ligase_ATP-dep_N.
IPR012340. NA-bd_OB-fold.
[Graphical view]
PfamiPF04679. DNA_ligase_A_C. 1 hit.
PF01068. DNA_ligase_A_M. 1 hit.
PF04675. DNA_ligase_A_N. 1 hit.
[Graphical view]
SUPFAMiSSF117018. SSF117018. 1 hit.
SSF50249. SSF50249. 1 hit.
TIGRFAMsiTIGR00574. dnl1. 1 hit.
PROSITEiPS00697. DNA_LIGASE_A1. 1 hit.
PS00333. DNA_LIGASE_A2. 1 hit.
PS50160. DNA_LIGASE_A3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O59288-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKYIELAQLY QKLEKTTMKL IKTRLVADFL KRVPEDHLDF IPYLILGDVF
60 70 80 90 100
PEWDERELGV GEKLLIKAVS MATGVDPKEI ENSVKDTGDL GESIALAIKR
110 120 130 140 150
RKQKSFFSQP LTIKRVYQTL VKIAETTGEG SQEKKMKYLA NLFMDAEPLE
160 170 180 190 200
AKYLARTVLG TMRTGVAEGL LRDAIALAFH VKVELVERAY MLTSDFGFVA
210 220 230 240 250
KIAKAEGNEG LEKVTIQLGK PIKPMLAQQA ANIKEALLEM GGEAEFEIKY
260 270 280 290 300
DGARVQVHKD GEKVIIYSRR LENVTRAIPE IVEAIKEAIE PEKVIVEGEL
310 320 330 340 350
VAIGEEGRPL PFQYVLRRFR RKHNIEEMMK KIPLELNLFD VLYVDGKSFV
360 370 380 390 400
DTKFIERRKK LEEIVKPNGK IKVAENLITK NAEEAEKFYK RALEMGHEGL
410 420 430 440 450
MAKRLDAVYE PGNRGKKWLK IKPTMENLDL VIIGAEWGEG RRAHLFGSFI
460 470 480 490 500
LGAYDPETGE FLEVGKVGSG FTDDDLVEFT KMLKPLIVKE EGKRVVIQPK
510 520 530 540 550
IVIEVTYQEI QKSPKYRSGF ALRFPRYVAL RDDKGPEDAD TIERIAQLYE

LQERIKGKV
Length:559
Mass (Da):63,623
Last modified:August 1, 1998 - v1
Checksum:iD1E85172B1D72188
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000001 Genomic DNA. Translation: BAA30734.1.
PIRiF71041.
RefSeqiNP_143476.1. NC_000961.1.

Genome annotation databases

EnsemblBacteriaiBAA30734; BAA30734; BAA30734.
GeneIDi1442475.
KEGGipho:PH1622.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000001 Genomic DNA. Translation: BAA30734.1 .
PIRi F71041.
RefSeqi NP_143476.1. NC_000961.1.

3D structure databases

ProteinModelPortali O59288.
SMRi O59288. Positions 1-559.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 70601.PH1622.

Proteomic databases

PRIDEi O59288.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAA30734 ; BAA30734 ; BAA30734 .
GeneIDi 1442475.
KEGGi pho:PH1622.

Phylogenomic databases

eggNOGi COG1793.
HOGENOMi HOG000036008.
KOi K10747.
OMAi ARVQVHK.

Enzyme and pathway databases

BioCyci PHOR70601:GJWR-1624-MONOMER.

Family and domain databases

Gene3Di 1.10.3260.10. 1 hit.
2.40.50.140. 1 hit.
HAMAPi MF_00407. DNA_ligase.
InterProi IPR022865. DNA_ligae_ATP-dep_bac/arc.
IPR000977. DNA_ligase_ATP-dep.
IPR012309. DNA_ligase_ATP-dep_C.
IPR012310. DNA_ligase_ATP-dep_cent.
IPR016059. DNA_ligase_ATP-dep_CS.
IPR012308. DNA_ligase_ATP-dep_N.
IPR012340. NA-bd_OB-fold.
[Graphical view ]
Pfami PF04679. DNA_ligase_A_C. 1 hit.
PF01068. DNA_ligase_A_M. 1 hit.
PF04675. DNA_ligase_A_N. 1 hit.
[Graphical view ]
SUPFAMi SSF117018. SSF117018. 1 hit.
SSF50249. SSF50249. 1 hit.
TIGRFAMsi TIGR00574. dnl1. 1 hit.
PROSITEi PS00697. DNA_LIGASE_A1. 1 hit.
PS00333. DNA_LIGASE_A2. 1 hit.
PS50160. DNA_LIGASE_A3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3.

Entry informationi

Entry nameiDNLI_PYRHO
AccessioniPrimary (citable) accession number: O59288
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: August 1, 1998
Last modified: October 29, 2014
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3