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Protein

DNA ligase

Gene

lig

Organism
Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair.UniRule annotation

Catalytic activityi

ATP + (deoxyribonucleotide)(n)-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)(m) = (deoxyribonucleotide)(n+m) + AMP + diphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei247ATPUniRule annotation1
Active sitei249N6-AMP-lysine intermediateUniRule annotation1
Binding sitei254ATPUniRule annotation1
Binding sitei269ATPUniRule annotation1
Binding sitei299ATPUniRule annotation1
Binding sitei339ATPUniRule annotation1
Binding sitei414ATPUniRule annotation1
Binding sitei420ATPUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
Biological processCell cycle, Cell division, DNA damage, DNA recombination, DNA repair, DNA replication
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciPHOR70601:G1G39-1575-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
DNA ligaseUniRule annotation (EC:6.5.1.1UniRule annotation)
Alternative name(s):
Polydeoxyribonucleotide synthase [ATP]UniRule annotation
Gene namesi
Name:ligUniRule annotation
Ordered Locus Names:PH1622
OrganismiPyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Taxonomic identifieri70601 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
Proteomesi
  • UP000000752 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000596141 – 559DNA ligaseAdd BLAST559

Proteomic databases

PRIDEiO59288

Interactioni

Protein-protein interaction databases

STRINGi70601.PH1622

Structurei

3D structure databases

ProteinModelPortaliO59288
SMRiO59288
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ATP-dependent DNA ligase family.UniRule annotation

Phylogenomic databases

eggNOGiarCOG01347 Archaea
COG1793 LUCA
HOGENOMiHOG000036008
KOiK10747
OMAiWLFEESY
OrthoDBiPOG093Z03L0

Family and domain databases

Gene3Di1.10.3260.10, 1 hit
HAMAPiMF_00407 DNA_ligase, 1 hit
InterProiView protein in InterPro
IPR022865 DNA_ligae_ATP-dep_bac/arc
IPR000977 DNA_ligase_ATP-dep
IPR012309 DNA_ligase_ATP-dep_C
IPR012310 DNA_ligase_ATP-dep_cent
IPR016059 DNA_ligase_ATP-dep_CS
IPR012308 DNA_ligase_ATP-dep_N
IPR036599 DNA_ligase_N_sf
IPR012340 NA-bd_OB-fold
PfamiView protein in Pfam
PF04679 DNA_ligase_A_C, 1 hit
PF01068 DNA_ligase_A_M, 1 hit
PF04675 DNA_ligase_A_N, 1 hit
SUPFAMiSSF117018 SSF117018, 1 hit
SSF50249 SSF50249, 1 hit
TIGRFAMsiTIGR00574 dnl1, 1 hit
PROSITEiView protein in PROSITE
PS00697 DNA_LIGASE_A1, 1 hit
PS00333 DNA_LIGASE_A2, 1 hit
PS50160 DNA_LIGASE_A3, 1 hit

Sequencei

Sequence statusi: Complete.

O59288-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKYIELAQLY QKLEKTTMKL IKTRLVADFL KRVPEDHLDF IPYLILGDVF
60 70 80 90 100
PEWDERELGV GEKLLIKAVS MATGVDPKEI ENSVKDTGDL GESIALAIKR
110 120 130 140 150
RKQKSFFSQP LTIKRVYQTL VKIAETTGEG SQEKKMKYLA NLFMDAEPLE
160 170 180 190 200
AKYLARTVLG TMRTGVAEGL LRDAIALAFH VKVELVERAY MLTSDFGFVA
210 220 230 240 250
KIAKAEGNEG LEKVTIQLGK PIKPMLAQQA ANIKEALLEM GGEAEFEIKY
260 270 280 290 300
DGARVQVHKD GEKVIIYSRR LENVTRAIPE IVEAIKEAIE PEKVIVEGEL
310 320 330 340 350
VAIGEEGRPL PFQYVLRRFR RKHNIEEMMK KIPLELNLFD VLYVDGKSFV
360 370 380 390 400
DTKFIERRKK LEEIVKPNGK IKVAENLITK NAEEAEKFYK RALEMGHEGL
410 420 430 440 450
MAKRLDAVYE PGNRGKKWLK IKPTMENLDL VIIGAEWGEG RRAHLFGSFI
460 470 480 490 500
LGAYDPETGE FLEVGKVGSG FTDDDLVEFT KMLKPLIVKE EGKRVVIQPK
510 520 530 540 550
IVIEVTYQEI QKSPKYRSGF ALRFPRYVAL RDDKGPEDAD TIERIAQLYE

LQERIKGKV
Length:559
Mass (Da):63,623
Last modified:August 1, 1998 - v1
Checksum:iD1E85172B1D72188
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000001 Genomic DNA Translation: BAA30734.1
PIRiF71041
RefSeqiWP_010885696.1, NC_000961.1

Genome annotation databases

EnsemblBacteriaiBAA30734; BAA30734; BAA30734
GeneIDi1442475
KEGGipho:PH1622

Similar proteinsi

Entry informationi

Entry nameiDNLI_PYRHO
AccessioniPrimary (citable) accession number: O59288
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: August 1, 1998
Last modified: April 25, 2018
This is version 120 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health