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O59251 (AMPPA_PYRHO) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 93. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
AMP phosphorylase

Short name=AMPpase
EC=2.4.2.57
Alternative name(s):
Nucleoside monophosphate phosphorylase
Short name=NMP phosphorylase
Gene names
Ordered Locus Names:PH1598
OrganismPyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3) [Complete proteome] [HAMAP]
Taxonomic identifier70601 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus

Protein attributes

Sequence length503 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the conversion of AMP and phosphate to adenine and ribose 1,5-bisphosphate (R15P). Exhibits phosphorylase activity toward CMP and UMP in addition to AMP. Functions in an archaeal AMP degradation pathway, together with R15P isomerase and RubisCO By similarity. HAMAP-Rule MF_02132

Catalytic activity

AMP + phosphate = adenine + alpha-D-ribose 1,5-bisphosphate. HAMAP-Rule MF_02132

CMP + phosphate = cytosine + alpha-D-ribose 1,5-bisphosphate. HAMAP-Rule MF_02132

UMP + phosphate = uracil + alpha-D-ribose 1,5-bisphosphate. HAMAP-Rule MF_02132

Sequence similarities

Belongs to the thymidine/pyrimidine-nucleoside phosphorylase family. Type 2 subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 503503AMP phosphorylase HAMAP-Rule MF_02132
PRO_0000059092

Regions

Nucleotide binding194 – 1996AMP By similarity

Sites

Active site2561Proton donor By similarity
Binding site1681AMP; via amide nitrogen By similarity
Binding site2031AMP; via amide nitrogen By similarity
Binding site2641AMP By similarity
Binding site2881AMP By similarity

Sequences

Sequence LengthMass (Da)Tools
O59251 [UniParc].

Last modified August 1, 1998. Version 1.
Checksum: 7DCE91203C847783

FASTA50353,977
        10         20         30         40         50         60 
MRAKVKILKI KTGSFNVFIS PRDAEEWKLH PNDLVKIESG KRSIYASVAI GDFIEDGEVG 

        70         80         90        100        110        120 
LSQDILSSYQ FSEGEVVSIT PSGTPESVKY IKKKMKGEKL RKVEIETIIR DIVDRKLRNT 

       130        140        150        160        170        180 
EISAFITAIE INGLSMDEIA ALTIAMAETG DMLDIDRKPI MDVHSIGGVP GNKTNILVVP 

       190        200        210        220        230        240 
IVAAAGLTIP KTSSRAITSA AGTADVVEVL TNVKLSLDEI KRIVEKIGAC LVWGGALNLA 

       250        260        270        280        290        300 
PADDLTIHVE RRLSLDPRGL MLASIMSKKY AIGSQYILID IPTGKGAKVE TMDEARTLAK 

       310        320        330        340        350        360 
DFIELGKKLG QYVEVAITYG GQPIGYAIGP ALEAKEALET LMTGKGPGSL VEKATGLAGI 

       370        380        390        400        410        420 
LLEMGGVAPK GMGKKVAKEI LESGKAYEKM KEIIEEQGGD PNIKPEDIPI GDKTYTIHAQ 

       430        440        450        460        470        480 
TGGYVTGIDN RAITAIAREA GAPEDKGAGV RLHVKVGEKV KEGDPLITIH AESESRLEKA 

       490        500 
IVLARRLEPI KIEGMVLQVI GNI 

« Hide

References

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BA000001 Genomic DNA. Translation: BAA30710.1.
PIRF71038.
RefSeqNP_143453.1. NC_000961.1.

3D structure databases

ProteinModelPortalO59251.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING70601.PH1598.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAA30710; BAA30710; BAA30710.
GeneID1442451.
KEGGpho:PH1598.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0213.
HOGENOMHOG000252767.
KOK00758.
OMAMVDLGKD.

Enzyme and pathway databases

BioCycPHOR70601:GJWR-1600-MONOMER.

Family and domain databases

Gene3D3.40.1030.10. 1 hit.
3.90.1170.30. 1 hit.
HAMAPMF_02132. AMP_phosphorylase.
InterProIPR017713. AMP_phosphorylase.
IPR009010. Asp_de-COase-like_dom.
IPR000312. Glycosyl_Trfase_fam3.
IPR017459. Glycosyl_Trfase_fam3_N_dom.
IPR013102. PYNP_C.
IPR000053. Pyrmidine_PPase.
IPR017872. Pyrmidine_PPase_CS.
IPR013466. Thymidine/AMP_Pase.
[Graphical view]
PANTHERPTHR10515. PTHR10515. 1 hit.
PfamPF02885. Glycos_trans_3N. 1 hit.
PF00591. Glycos_transf_3. 1 hit.
PF07831. PYNP_C. 1 hit.
[Graphical view]
PIRSFPIRSF000478. TP_PyNP. 1 hit.
SMARTSM00941. PYNP_C. 1 hit.
[Graphical view]
SUPFAMSSF47648. SSF47648. 1 hit.
SSF50692. SSF50692. 1 hit.
SSF52418. SSF52418. 1 hit.
SSF54680. SSF54680. 1 hit.
TIGRFAMsTIGR03327. AMP_phos. 1 hit.
TIGR02645. ARCH_P_rylase. 1 hit.
PROSITEPS00647. THYMID_PHOSPHORYLASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameAMPPA_PYRHO
AccessionPrimary (citable) accession number: O59251
Entry history
Integrated into UniProtKB/Swiss-Prot: August 29, 2003
Last sequence update: August 1, 1998
Last modified: May 14, 2014
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families