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Reviewed, UniProtKB/Swiss-Prot O59245 (Y1602_PYRHO)

Last modified November 24, 2009. Version 58. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    UPF0027 protein PH1602
Cleaved into the following chain:
    1- Recommended name:
            Pho hyp2 intein
              EC=3.1.-.-
Gene names
Ordered Locus Names: PH1602
OrganismPyrococcus horikoshii [Complete proteome] [HAMAP]
Taxonomic identifier53953 [NCBI]
Taxonomic lineageArchaea › Euryarchaeota › Thermococci › Thermococcales › Thermococcaceae › Pyrococcus

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Protein attributes

Sequence length871 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Subunit structure

Monomer. Ref.2

Post-translational modification

This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation Potential.

Sequence similarities

Belongs to the UPF0027 (rtcB) family.

Contains 1 DOD-type homing endonuclease domain.

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Ontologies

Keywords
   Biological processIntron homing
   LigandDNA-binding
   Molecular functionEndonuclease
Hydrolase
Nuclease
   PTMAutocatalytic cleavage
Protein splicing
   Technical term3D-structure
Complete proteome
Gene Ontology (GO)
   Biological processintein-mediated protein splicing

Inferred from electronic annotation. Source: InterPro

intron homing

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular functionDNA binding

Inferred from electronic annotation. Source: UniProtKB-KW

endonuclease activity

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 9797UPF0027 protein PH1602, 1st part
PRO_0000232535
Chain98 – 487390Pho hyp2 intein
PRO_0000232536
Chain488 – 871384UPF0027 protein PH1602, 2nd part
PRO_0000232537

Regions

Domain248 – 374127DOD-type homing endonuclease

Secondary structure

1 ........................................................................... 871
Helix Strand Turn

Details...

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Sequences

Sequence LengthMass (Da)Tools
O59245-1 [UniParc].

Last modified August 1, 1998. Version 1.
Checksum: 989723B0089288F4

FASTA87198,125
        10         20         30         40         50         60 
MVVPLKRIDK IRWEIPKFDK RMRVPGRVYA DEVLLEKMKN DRTLEQATNV AMLPGIYKYS 

        70         80         90        100        110        120 
IVMPDGHQGY GFPIGGVAAF DVKEGVISPG GIGYDINCLA PGTRVLTEHG YWLKIEEMPE 

       130        140        150        160        170        180 
KFKLQRLRVY NIEEGHNDFS KVVFVAEREV GSEEKAIRIV TESGKVIEGS EDHPVLTPEG 

       190        200        210        220        230        240 
YVYLRNVKEG DYILVYPFEG VPYEEKKGVI LDESAFEGED PQVVKFLRER NLIPLQWKDP 

       250        260        270        280        290        300 
KVGILARILG FALANGYISE NDNLTFHGKE EVLREVRKDL EELGIEAIVA EEDKLKVTSR 

       310        320        330        340        350        360 
EFAFLLEKLG MAHDSIPEWI IEGPLWIKRN FLAGLFGANG SIVEFKGDVP LPITLTHSRE 

       370        380        390        400        410        420 
LLNDVSRILE GFKVRAKIKM GKNGSYQLVI EDEDSIRNFL GRINYEYDPE KKARGLIAYA 

       430        440        450        460        470        480 
YLKFKELMKG NLMTFEEFAR DRGYEGGFVA EKVIEVKSVK PEYDKFYDIG VYHSAHNFIA 

       490        500        510        520        530        540 
NGIVVHNCGV RLIRTNLTEK EVRPRIKQLV DTLFKNVPSG VGSQGRIKLH WTQIDDVLVD 

       550        560        570        580        590        600 
GAKWAVDNGY GWERDLERLE EGGRMEGADP EAVSQRAKQR GAPQLGSLGS GNHFLEVQVV 

       610        620        630        640        650        660 
DKIFDPEVAK AYGLFEGQVV VMVHTGSRGL GHQVASDYLR IMERAIRKYR IPWPDRELVS 

       670        680        690        700        710        720 
VPFQSEEGQR YFSAMKAAAN FAWANRQMIT HWVRESFQEV FKQDPEGDLG MDIVYDVAHN 

       730        740        750        760        770        780 
IGKVEEHEVD GKRVKVIVHR KGATRAFPPG HEAVPRLYRD VGQPVLIPGS MGTASYILAG 

       790        800        810        820        830        840 
TEGAMKETFG STCHGAGRVL SRKAATRQYR GDRIRQELLN RGIYVRAASM RVVAEEAPGA 

       850        860        870 
YKNVDNVVKV VSEAGIAKLV ARMRPIGVAK G

« Hide

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References

« Hide 'large scale' references
[1]"Complete sequence and gene organization of the genome of a hyper-thermophilic archaebacterium, Pyrococcus horikoshii OT3."
Kawarabayasi Y., Sawada M., Horikawa H., Haikawa Y., Hino Y., Yamamoto S., Sekine M., Baba S., Kosugi H., Hosoyama A., Nagai Y., Sakai M., Ogura K., Otsuka R., Nakazawa H., Takamiya M., Ohfuku Y., Funahashi T. expand/collapse author list , Tanaka T., Kudoh Y., Yamazaki J., Kushida N., Oguchi A., Aoki K., Yoshizawa T., Nakamura Y., Robb F.T., Horikoshi K., Masuchi Y., Shizuya H., Kikuchi H.
DNA Res. 5:55-76(1998) [PubMed: 9679194] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: OT3.
[2]"Crystal structure of an RtcB homolog protein (PH1602-extein protein) from Pyrococcus horikoshii reveals a novel fold."
Okada C., Maegawa Y., Yao M., Tanaka I.
Proteins 63:1119-1122(2006) [PubMed: 16485279] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.15 ANGSTROMS) OF 1-97 AND 488-871, SUBUNIT.

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Cross-references

Sequence databases

BA000001 Genomic DNA. Translation: BAA30714.1.
PIRB71039.
RefSeqNP_143457.1.

3D structure databases

EntryMethodResolution (Å)ChainPositionsPDBsum
1UC2X-ray2.15A/B1-871[»]
SMRO59245. Positions 2-98.
ModBaseSearch...

Genome annotation databases

GeneID1442456.
GenomeReviewsGene locus PH1602 in contig BA000001_GR.
KEGGpho:PH1602.
NMPDRfig|70601.1.peg.1570.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMO59245.
OMAVHNCGVR

Enzyme and pathway databases

BioCycPHOR70601:PH1602-MON.

Family and domain databases

InterProIPR003586. Hedgehog_hint_C.
IPR003587. Hedgehog_hint_N.
IPR006142. INTEIN.
IPR004042. Intein_endonuc.
IPR006141. Intein_splicing_site.
IPR001233. UPF0027.
[Graphical view]
PANTHERPTHR11118. UPF0027. 1 hit.
PfamPF01139. UPF0027. 2 hits.
[Graphical view]
PRINTSPR00379. INTEIN.
SMARTSM00305. HintC. 1 hit.
SM00306. HintN. 1 hit.
[Graphical view]
TIGRFAMsTIGR01443. intein_Cterm. 1 hit.
TIGR01445. intein_Nterm. 1 hit.
PROSITEPS50818. INTEIN_C_TER. 1 hit.
PS50819. INTEIN_ENDONUCLEASE. 1 hit.
PS50817. INTEIN_N_TER. 1 hit.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry nameY1602_PYRHO
AccessionPrimary (citable) accession number: O59245
Entry history
Integrated into UniProtKB/Swiss-Prot: April 18, 2006
Last sequence update: August 1, 1998
Last modified: November 24, 2009
This is version 58 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

Intein-containing proteins

List of intein-containing protein entries

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Uncharacterized protein families (UPF)

List of uncharacterized protein family (UPF) entries

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents