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Protein

Glycine--tRNA ligase

Gene

glyS

Organism
Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of glycine to tRNA(Gly).UniRule annotation

Catalytic activityi

ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-tRNA(Gly).UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei99SubstrateUniRule annotation1
Binding sitei165SubstrateUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi197 – 199ATPUniRule annotation3
Nucleotide bindingi207 – 212ATPUniRule annotation6
Nucleotide bindingi324 – 325ATPUniRule annotation2
Nucleotide bindingi443 – 446ATPUniRule annotation4

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciPHOR70601:G1G39-1566-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Glycine--tRNA ligaseUniRule annotation (EC:6.1.1.14UniRule annotation)
Alternative name(s):
Glycyl-tRNA synthetaseUniRule annotation
Short name:
GlyRSUniRule annotation
Gene namesi
Name:glySUniRule annotation
Ordered Locus Names:PH1614
OrganismiPyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Taxonomic identifieri70601 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
Proteomesi
  • UP000000752 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000729961 – 570Glycine--tRNA ligaseAdd BLAST570

Proteomic databases

PRIDEiO59235

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

STRINGi70601.PH1614

Structurei

3D structure databases

ProteinModelPortaliO59235
SMRiO59235
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni212 – 216Substrate bindingUniRule annotation5
Regioni439 – 443Substrate bindingUniRule annotation5

Sequence similaritiesi

Belongs to the class-II aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiarCOG00405 Archaea
COG0423 LUCA
HOGENOMiHOG000242015
KOiK01880
OMAiEPSYGID
OrthoDBiPOG093Z01L8

Family and domain databases

CDDicd00774 GlyRS-like_core, 1 hit
Gene3Di3.40.50.800, 1 hit
HAMAPiMF_00253_A Gly_tRNA_synth_A, 1 hit
InterProiView protein in InterPro
IPR002314 aa-tRNA-synt_IIb
IPR006195 aa-tRNA-synth_II
IPR004154 Anticodon-bd
IPR036621 Anticodon-bd_dom_sf
IPR027031 Gly-tRNA_synthase/POLG2
IPR022960 Gly_tRNA_ligase_arc
IPR033731 GlyRS-like_core
IPR002315 tRNA-synt_gly
PANTHERiPTHR10745 PTHR10745, 1 hit
PfamiView protein in Pfam
PF03129 HGTP_anticodon, 1 hit
PF00587 tRNA-synt_2b, 1 hit
PRINTSiPR01043 TRNASYNTHGLY
TIGRFAMsiTIGR00389 glyS_dimeric, 1 hit
PROSITEiView protein in PROSITE
PS50862 AA_TRNA_LIGASE_II, 1 hit

Sequencei

Sequence statusi: Complete.

O59235-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNGKFDKYEY LQDLMRRRGF AWGSFEIYGG SRGFYDYGPL GATIKRKIER
60 70 80 90 100
KIREAFIREG FFEIETPDIT PEQVFIASGH VEKFVDPVVE CKKCGMRFRA
110 120 130 140 150
DHLIEEFLGI DVEGKSAEEM SRIIREHGLK CPECGGELSD VFYFNLMFET
160 170 180 190 200
YIGPYKDKKA YLRPETAQGI FVNFKRLNAF ARNKLPFGVF QIGKAYRNEI
210 220 230 240 250
SPRQGMIRLR EFTQAEAEIF FNPNETEHPH FNEVKHEKLK LYPIENQLKE
260 270 280 290 300
LGEVEVTAEE AVKRGYVMNS FFAYYMVMIK KILLDIGIPE DKIRFRQQLP
310 320 330 340 350
EERAHYSADT WDAEVYSERF GWVECVGLAY RTDYDLSRHM KMSGADLTVM
360 370 380 390 400
IHYDKPKVVK RIQVSLNMKK VGPKLKADAK KINEIIKSMD QKELEKLVKD
410 420 430 440 450
LNEKGKVTIE GYELSKEDFI VKEVEEKITG EKIVPHVLEP SFGIDRPFYL
460 470 480 490 500
LLENSLTVDE DGRIYLKIKK DMAPIEVAVL PLVAKEPLTT IAYEIYRTLQ
510 520 530 540 550
KEGFIVVYDE KDSIGKRYMR YDEIGTPYCV TVDNQTPEDG TVTIRDRDTR
560 570
EQIRVKIEEL PRKLKELIFG
Length:570
Mass (Da):66,459
Last modified:August 1, 1998 - v1
Checksum:i6A6E5CEE776302FF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000001 Genomic DNA Translation: BAA30726.1
PIRiF71040
RefSeqiWP_010885687.1, NC_000961.1

Genome annotation databases

EnsemblBacteriaiBAA30726; BAA30726; BAA30726
GeneIDi1442466
KEGGipho:PH1614

Similar proteinsi

Entry informationi

Entry nameiSYG_PYRHO
AccessioniPrimary (citable) accession number: O59235
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: August 1, 1998
Last modified: May 23, 2018
This is version 114 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families
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