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Protein

Glycine--tRNA ligase

Gene

glyS

Organism
Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the attachment of glycine to tRNA(Gly).UniRule annotation

Catalytic activityi

ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-tRNA(Gly).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei99 – 991SubstrateUniRule annotation
Binding sitei165 – 1651SubstrateUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi197 – 1993ATPUniRule annotation
Nucleotide bindingi207 – 2126ATPUniRule annotation
Nucleotide bindingi324 – 3252ATPUniRule annotation
Nucleotide bindingi443 – 4464ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciPHOR70601:GJWR-1615-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Glycine--tRNA ligaseUniRule annotation (EC:6.1.1.14UniRule annotation)
Alternative name(s):
Glycyl-tRNA synthetaseUniRule annotation
Short name:
GlyRSUniRule annotation
Gene namesi
Name:glySUniRule annotation
Ordered Locus Names:PH1614
OrganismiPyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Taxonomic identifieri70601 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
Proteomesi
  • UP000000752 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 570570Glycine--tRNA ligasePRO_0000072996Add
BLAST

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

STRINGi70601.PH1614.

Structurei

3D structure databases

ProteinModelPortaliO59235.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni212 – 2165Substrate bindingUniRule annotation
Regioni439 – 4435Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the class-II aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiarCOG00405. Archaea.
COG0423. LUCA.
HOGENOMiHOG000242015.
KOiK01880.
OMAiERFGWVE.

Family and domain databases

CDDicd00774. GlyRS-like_core. 1 hit.
Gene3Di3.40.50.800. 1 hit.
HAMAPiMF_00253_A. Gly_tRNA_synth_A. 1 hit.
InterProiIPR002314. aa-tRNA-synt_IIb.
IPR006195. aa-tRNA-synth_II.
IPR004154. Anticodon-bd.
IPR027031. Gly-tRNA_synthase/POLG2.
IPR022960. Gly_tRNA_ligase_arc.
IPR033731. GlyRS-like_core.
IPR002315. tRNA-synt_gly.
[Graphical view]
PANTHERiPTHR10745. PTHR10745. 1 hit.
PfamiPF03129. HGTP_anticodon. 1 hit.
PF00587. tRNA-synt_2b. 1 hit.
[Graphical view]
PRINTSiPR01043. TRNASYNTHGLY.
SUPFAMiSSF52954. SSF52954. 1 hit.
TIGRFAMsiTIGR00389. glyS_dimeric. 1 hit.
PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O59235-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNGKFDKYEY LQDLMRRRGF AWGSFEIYGG SRGFYDYGPL GATIKRKIER
60 70 80 90 100
KIREAFIREG FFEIETPDIT PEQVFIASGH VEKFVDPVVE CKKCGMRFRA
110 120 130 140 150
DHLIEEFLGI DVEGKSAEEM SRIIREHGLK CPECGGELSD VFYFNLMFET
160 170 180 190 200
YIGPYKDKKA YLRPETAQGI FVNFKRLNAF ARNKLPFGVF QIGKAYRNEI
210 220 230 240 250
SPRQGMIRLR EFTQAEAEIF FNPNETEHPH FNEVKHEKLK LYPIENQLKE
260 270 280 290 300
LGEVEVTAEE AVKRGYVMNS FFAYYMVMIK KILLDIGIPE DKIRFRQQLP
310 320 330 340 350
EERAHYSADT WDAEVYSERF GWVECVGLAY RTDYDLSRHM KMSGADLTVM
360 370 380 390 400
IHYDKPKVVK RIQVSLNMKK VGPKLKADAK KINEIIKSMD QKELEKLVKD
410 420 430 440 450
LNEKGKVTIE GYELSKEDFI VKEVEEKITG EKIVPHVLEP SFGIDRPFYL
460 470 480 490 500
LLENSLTVDE DGRIYLKIKK DMAPIEVAVL PLVAKEPLTT IAYEIYRTLQ
510 520 530 540 550
KEGFIVVYDE KDSIGKRYMR YDEIGTPYCV TVDNQTPEDG TVTIRDRDTR
560 570
EQIRVKIEEL PRKLKELIFG
Length:570
Mass (Da):66,459
Last modified:August 1, 1998 - v1
Checksum:i6A6E5CEE776302FF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000001 Genomic DNA. Translation: BAA30726.1.
PIRiF71040.
RefSeqiWP_010885687.1. NC_000961.1.

Genome annotation databases

EnsemblBacteriaiBAA30726; BAA30726; BAA30726.
GeneIDi1442466.
KEGGipho:PH1614.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000001 Genomic DNA. Translation: BAA30726.1.
PIRiF71040.
RefSeqiWP_010885687.1. NC_000961.1.

3D structure databases

ProteinModelPortaliO59235.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi70601.PH1614.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAA30726; BAA30726; BAA30726.
GeneIDi1442466.
KEGGipho:PH1614.

Phylogenomic databases

eggNOGiarCOG00405. Archaea.
COG0423. LUCA.
HOGENOMiHOG000242015.
KOiK01880.
OMAiERFGWVE.

Enzyme and pathway databases

BioCyciPHOR70601:GJWR-1615-MONOMER.

Family and domain databases

CDDicd00774. GlyRS-like_core. 1 hit.
Gene3Di3.40.50.800. 1 hit.
HAMAPiMF_00253_A. Gly_tRNA_synth_A. 1 hit.
InterProiIPR002314. aa-tRNA-synt_IIb.
IPR006195. aa-tRNA-synth_II.
IPR004154. Anticodon-bd.
IPR027031. Gly-tRNA_synthase/POLG2.
IPR022960. Gly_tRNA_ligase_arc.
IPR033731. GlyRS-like_core.
IPR002315. tRNA-synt_gly.
[Graphical view]
PANTHERiPTHR10745. PTHR10745. 1 hit.
PfamiPF03129. HGTP_anticodon. 1 hit.
PF00587. tRNA-synt_2b. 1 hit.
[Graphical view]
PRINTSiPR01043. TRNASYNTHGLY.
SUPFAMiSSF52954. SSF52954. 1 hit.
TIGRFAMsiTIGR00389. glyS_dimeric. 1 hit.
PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSYG_PYRHO
AccessioniPrimary (citable) accession number: O59235
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: August 1, 1998
Last modified: September 7, 2016
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.