O59226 (O59226_PYRHO) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
DNA-directed RNA polymerase RuleBase RU000431
EC=2.7.7.6 RuleBase RU000431

Gene names

Ordered Locus Names:

PH1546

Organism

Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3) [Complete proteome] [HAMAP]

Taxonomic identifier

70601 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Thermococci › Thermococcales › Thermococcaceae › Pyrococcus

Protein attributes

Sequence length	1117 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1). RuleBase RU000431
Sequence similarities	Belongs to the RNA polymerase beta chain family. RuleBase RU000434

Ontologies

Keywords
Biological process	Transcription
Cellular component	DNA-directed RNA polymerase RuleBase RU000431 EMBL BAA30657.1
Molecular function	Nucleotidyltransferase RuleBase RU000431 Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro DNA-directed RNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW electron carrier activity Inferred from electronic annotation. Source: InterPro ribonucleoside binding Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	O59226 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: C42DBDBE2CF74241	FASTA	1,117	127,089
	10 20 30 40 50 60 MRGPTVVEVT PDDLWIVMES YWEEKGLVRQ HLDSYNAFIE RGLQEVVDEF GGIKPDIPDF 70 80 90 100 110 120 EVKFGKIRVG EPEFQEPHGQ RKPLYPMDAR IRNLTYSAPL YLEMIPVIRG IEQDPVEVRI 130 140 150 160 170 180 GELPIMLKSK ACRLYGLSDE ELIKLGEDPK DPGGYFIING SERVIVSIED IAPNRTLVEK 190 200 210 220 230 240 DERQEKYIAK VFSYRHGYRA LVTVERKKDG ILYVGIPNVP RPVKFVYVMR ALGLERDKDI 250 260 270 280 290 300 VDAVGNDPEV QQILFDNLED ASDITTQQEA LEYIGKLVAP GQAREYRLKR AEYVIDNNLL 310 320 330 340 350 360 PHMGVTPEDR IRKAYYLGMM ALKVIELSLG KRDEDDKDHY ANKRLRLAGD LLKDLFRVAF 370 380 390 400 410 420 SQLIKDIQYQ MTKTYQRRGE KYVFSNIHRF IRNSIRPDVL TERIEHALAT GAWPGGRTGV 430 440 450 460 470 480 SQLLDRTNYV STLSHLRRVT SPLDREQPHF EARDLHGTHW GRICPTETPE GPNCGLVKNL 490 500 510 520 530 540 ALMAQITTAV PEEEVREYLM SLGIVPIEVR RPSPELWRVY LNGVLIGTVE DGKALVERIK 550 560 570 580 590 600 KDRRSGKISD IINVAYYEDV KEVYVNSDDG RVRRPLIIVE NGVPKLTKEH VQAIKEGRLK 610 620 630 640 650 660 WSDLVRMGVI EYLDAEEEEN AYVAMWPWEV TKEHTHLELM PAAILGLPAS LIPYPEHNAA 670 680 690 700 710 720 PRNTYGAGMA KQSLGLGWAN FRIRVDTRGH LLHYPQIPLV NSRIMKAVGY EDRPAGQNFV 730 740 750 760 770 780 VAVLAYHGYN MEDAIIINKA SIERGLARST FFRTYEAEEK KYLGGQTDRF EIPAPTVRGY 790 800 810 820 830 840 RGEKYYRHLD EDGLIFPESK VEGKDVLIGR TSPPRFLEER GLGAVSLQER RETSVAVRPS 850 860 870 880 890 900 EKGIVDKVII TETGDGTKLV KVTVRDLRIP EIGDKFASRH GQKGVIGLIV PQEDMPWTES 910 920 930 940 950 960 GIVPDLIVNP HGIPSRMTVG QLLEAIGGKV AALKGRRVDG TAFIGEPEEK LRKELEELGF 970 980 990 1000 1010 1020 KHTGREIMYD GITGKRFEAD IFIGIIYYQR LHHMVADKIH ARSRGPVQVL TKQPTEGRAR 1030 1040 1050 1060 1070 1080 EGGLRFGEME RDVLVGHGAA MLLIERLLEE SDKTEVWVCE NCGHIAIEDK RRHKVYCPVC 1090 1100 1110 GEEERISKVE MSYAFKLLLD ELKAMVIRPK LNLTERV

References

[1]

"Complete sequence and gene organization of the genome of a hyper-thermophilic archaebacterium, Pyrococcus horikoshii OT3." Kawarabayasi Y., Sawada M., Horikawa H., Haikawa Y., Hino Y., Yamamoto S., Sekine M., Baba S., Kosugi H., Hosoyama A., Nagai Y., Sakai M., Ogura K., Otsuka R., Nakazawa H., Takamiya M., Ohfuku Y., Funahashi T. , Tanaka T., Kudoh Y., Yamazaki J., Kushida N., Oguchi A., Aoki K., Kikuchi H. DNA Res. 5:55-76(1998) [PubMed: 9679194] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3 and OT3.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000001 Genomic DNA. Translation: BAA30657.1.
PIR	A71032.
RefSeq	NP_143407.1. NC_000961.1.
3D structure databases
HSSP	HSSP built from PDB template 1I50 based on UniProtKB P08518.
ProteinModelPortal	O59226.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBPYRT00000000187; EBPYRP00000000187; EBPYRG00000000187.
GeneID	1443865.
GenomeReviews	Gene locus PH1546 in contig BA000001_GR.
KEGG	pho:PH1546.
NMPDR	fig|70601.1.peg.1518.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000022647.
HOGENOM	HBG317648.
OMA	FGPTYYQ.
PhylomeDB	O59226.
ProtClustDB	PRK08565.
Enzyme and pathway databases
BioCyc	PHOR70601:PH1546-MONOMER.
Family and domain databases
InterPro	IPR015712. DNA-dir_RNA_pol_su2. IPR007120. DNA-dir_RNA_pol_su2_6. IPR019969. DNA-dir_RNA_pol_su_B_arc. IPR007121. RNA_pol_bsu_CS. IPR007644. RNA_pol_bsu_protrusion. IPR007642. RNA_pol_Rpb2_2. IPR007645. RNA_pol_Rpb2_3. IPR007646. RNA_pol_Rpb2_4. IPR007647. RNA_pol_Rpb2_5. IPR007641. RNA_pol_Rpb2_7. IPR014724. RNA_pol_RPB2_OB-fold. IPR004039. Rubredoxin-type_fold. [Graphical view]
Gene3D	G3DSA:2.40.270.10. G3DSA:2.40.270.10. 2 hits. G3DSA:2.40.50.150. Ribosomal_L2. 1 hit. G3DSA:2.20.28.10. Rubredox. 1 hit.
KO	K13798.
PANTHER	PTHR20856. RNA_pol_I_sub2. 1 hit.
Pfam	PF04563. RNA_pol_Rpb2_1. 1 hit. PF04561. RNA_pol_Rpb2_2. 1 hit. PF04565. RNA_pol_Rpb2_3. 1 hit. PF04566. RNA_pol_Rpb2_4. 1 hit. PF04567. RNA_pol_Rpb2_5. 1 hit. PF00562. RNA_pol_Rpb2_6. 1 hit. PF04560. RNA_pol_Rpb2_7. 1 hit. [Graphical view]
TIGRFAMs	TIGR03670. RpoB_arch. 1 hit.
PROSITE	PS01166. RNA_POL_BETA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

O59226_PYRHO

Accession

Primary (citable) accession number: O59226

Entry history

Integrated into UniProtKB/TrEMBL:	August 1, 1998
Last sequence update:	August 1, 1998
Last modified:	January 25, 2012
This is version 74 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)