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O59198

- RTCA_PYRHO

UniProt

O59198 - RTCA_PYRHO

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Protein

RNA 3'-terminal phosphate cyclase

Gene

rtcA

Organism
Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Catalyzes the conversion of 3'-phosphate to a 2',3'-cyclic phosphodiester at the end of RNA. The mechanism of action of the enzyme occurs in 3 steps: (A) adenylation of the enzyme by ATP; (B) transfer of adenylate to an RNA-N3'P to produce RNA-N3'PP5'A; (C) and attack of the adjacent 2'-hydroxyl on the 3'-phosphorus in the diester linkage to produce the cyclic end product. The biological role of this enzyme is unknown but it is likely to function in some aspects of cellular RNA processing (By similarity).By similarity

Catalytic activityi

ATP + RNA 3'-terminal-phosphate = AMP + diphosphate + RNA terminal-2',3'-cyclic-phosphate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei100 – 1001ATPBy similarity
Active sitei307 – 3071Tele-AMP-histidine intermediateBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi283 – 2875ATPBy similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. RNA-3'-phosphate cyclase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. RNA processing Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciPHOR70601:GJWR-1530-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
RNA 3'-terminal phosphate cyclase (EC:6.5.1.4)
Short name:
RNA cyclase
Short name:
RNA-3'-phosphate cyclase
Gene namesi
Name:rtcA
Ordered Locus Names:PH1529
ORF Names:PHCV028
OrganismiPyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Taxonomic identifieri70601 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
ProteomesiUP000000752: Chromosome

Subcellular locationi

Cytoplasm Curated

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 341341RNA 3'-terminal phosphate cyclasePRO_0000156433Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi70601.PH1529.

Structurei

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4O89X-ray1.90A/B1-341[»]
4O8JX-ray2.04A/B1-341[»]
ProteinModelPortaliO59198.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0430.
HOGENOMiHOG000015264.
KOiK18105.
OMAiERTRTGH.

Family and domain databases

Gene3Di3.30.360.20. 1 hit.
3.65.10.20. 2 hits.
HAMAPiMF_00200. RTC.
InterProiIPR013791. RNA3'-term_phos_cycl_insert.
IPR023797. RNA3'_phos_cyclase_dom.
IPR000228. RNA3'_term_phos_cyc.
IPR017770. RNA3'_term_phos_cyc_type_1.
IPR020719. RNA3'_term_phos_cycl-like_CS.
IPR013792. RNA3'P_cycl/enolpyr_Trfase_a/b.
[Graphical view]
PANTHERiPTHR11096. PTHR11096. 1 hit.
PfamiPF01137. RTC. 1 hit.
PF05189. RTC_insert. 1 hit.
[Graphical view]
SUPFAMiSSF55205. SSF55205. 1 hit.
TIGRFAMsiTIGR03399. RNA_3prim_cycl. 1 hit.
PROSITEiPS01287. RTC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O59198-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MITIDGSYGE GGGQILRTSV ALSTITGEPV RIVNIRANRP NPGLRPQHLH
60 70 80 90 100
AILALKHLAN AEVKGAHVGS RELVFIPKKL EAKEISIDIG TAGSITLVLQ
110 120 130 140 150
ALLPAMVFAR EKVKFRITGG TDVSWSPPVD YLSNVTLFAL EKIGIHGEIR
160 170 180 190 200
VIRRGHYPKG GGIVEGYVEP WNEKRELVAK EYSRIIKIEG ISHATNLPSH
210 220 230 240 250
VAERQARAAK DELLQLKVPI EIRTEISRSI GPGSGIVVWA ETDCLRLGGD
260 270 280 290 300
ALGKKGKPAE IVGKEAAQEL LDQLKPGHCV DKFLGDQLIP FLAFSGGVIW
310 320 330 340
VSEITNHLKT NIWVVESFLG RIFDVDGNVG EPGKIRVIRR V
Length:341
Mass (Da):37,167
Last modified:December 1, 2000 - v2
Checksum:i1E24A4F6BF9FAC29
GO

Sequence cautioni

The sequence BAA30639.1 differs from that shown. Reason: Erroneous initiation.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000001 Genomic DNA. Translation: BAA30639.1. Different initiation.
PIRiG71029.
RefSeqiNP_143389.1. NC_000961.1.

Genome annotation databases

EnsemblBacteriaiBAA30639; BAA30639; BAA30639.
GeneIDi1443845.
KEGGipho:PH1529.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000001 Genomic DNA. Translation: BAA30639.1 . Different initiation.
PIRi G71029.
RefSeqi NP_143389.1. NC_000961.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
4O89 X-ray 1.90 A/B 1-341 [» ]
4O8J X-ray 2.04 A/B 1-341 [» ]
ProteinModelPortali O59198.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 70601.PH1529.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAA30639 ; BAA30639 ; BAA30639 .
GeneIDi 1443845.
KEGGi pho:PH1529.

Phylogenomic databases

eggNOGi COG0430.
HOGENOMi HOG000015264.
KOi K18105.
OMAi ERTRTGH.

Enzyme and pathway databases

BioCyci PHOR70601:GJWR-1530-MONOMER.

Family and domain databases

Gene3Di 3.30.360.20. 1 hit.
3.65.10.20. 2 hits.
HAMAPi MF_00200. RTC.
InterProi IPR013791. RNA3'-term_phos_cycl_insert.
IPR023797. RNA3'_phos_cyclase_dom.
IPR000228. RNA3'_term_phos_cyc.
IPR017770. RNA3'_term_phos_cyc_type_1.
IPR020719. RNA3'_term_phos_cycl-like_CS.
IPR013792. RNA3'P_cycl/enolpyr_Trfase_a/b.
[Graphical view ]
PANTHERi PTHR11096. PTHR11096. 1 hit.
Pfami PF01137. RTC. 1 hit.
PF05189. RTC_insert. 1 hit.
[Graphical view ]
SUPFAMi SSF55205. SSF55205. 1 hit.
TIGRFAMsi TIGR03399. RNA_3prim_cycl. 1 hit.
PROSITEi PS01287. RTC. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3.

Entry informationi

Entry nameiRTCA_PYRHO
AccessioniPrimary (citable) accession number: O59198
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: December 1, 2000
Last modified: October 29, 2014
This is version 85 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3