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O59198

- RTCA_PYRHO

UniProt

O59198 - RTCA_PYRHO

Protein

RNA 3'-terminal phosphate cyclase

Gene

rtcA

Organism
Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 84 (01 Oct 2014)
      Sequence version 2 (01 Dec 2000)
      Previous versions | rss
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    Functioni

    Catalyzes the conversion of 3'-phosphate to a 2',3'-cyclic phosphodiester at the end of RNA. The mechanism of action of the enzyme occurs in 3 steps: (A) adenylation of the enzyme by ATP; (B) transfer of adenylate to an RNA-N3'P to produce RNA-N3'PP5'A; (C) and attack of the adjacent 2'-hydroxyl on the 3'-phosphorus in the diester linkage to produce the cyclic end product. The biological role of this enzyme is unknown but it is likely to function in some aspects of cellular RNA processing By similarity.By similarity

    Catalytic activityi

    ATP + RNA 3'-terminal-phosphate = AMP + diphosphate + RNA terminal-2',3'-cyclic-phosphate.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei100 – 1001ATPBy similarity
    Active sitei307 – 3071Tele-AMP-histidine intermediateBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi283 – 2875ATPBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. RNA-3'-phosphate cyclase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. RNA processing Source: InterPro

    Keywords - Molecular functioni

    Ligase

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciPHOR70601:GJWR-1530-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    RNA 3'-terminal phosphate cyclase (EC:6.5.1.4)
    Short name:
    RNA cyclase
    Short name:
    RNA-3'-phosphate cyclase
    Gene namesi
    Name:rtcA
    Ordered Locus Names:PH1529
    ORF Names:PHCV028
    OrganismiPyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
    Taxonomic identifieri70601 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
    ProteomesiUP000000752: Chromosome

    Subcellular locationi

    Cytoplasm Curated

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 341341RNA 3'-terminal phosphate cyclasePRO_0000156433Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi70601.PH1529.

    Structurei

    3D structure databases

    ProteinModelPortaliO59198.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0430.
    HOGENOMiHOG000015264.
    KOiK18105.
    OMAiERTRTGH.

    Family and domain databases

    Gene3Di3.30.360.20. 1 hit.
    3.65.10.20. 2 hits.
    HAMAPiMF_00200. RTC.
    InterProiIPR013791. RNA3'-term_phos_cycl_insert.
    IPR023797. RNA3'_phos_cyclase_dom.
    IPR000228. RNA3'_term_phos_cyc.
    IPR017770. RNA3'_term_phos_cyc_type_1.
    IPR020719. RNA3'_term_phos_cycl-like_CS.
    IPR013792. RNA3'P_cycl/enolpyr_Trfase_a/b.
    [Graphical view]
    PANTHERiPTHR11096. PTHR11096. 1 hit.
    PfamiPF01137. RTC. 1 hit.
    PF05189. RTC_insert. 1 hit.
    [Graphical view]
    SUPFAMiSSF55205. SSF55205. 1 hit.
    TIGRFAMsiTIGR03399. RNA_3prim_cycl. 1 hit.
    PROSITEiPS01287. RTC. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O59198-1 [UniParc]FASTAAdd to Basket

    « Hide

    MITIDGSYGE GGGQILRTSV ALSTITGEPV RIVNIRANRP NPGLRPQHLH    50
    AILALKHLAN AEVKGAHVGS RELVFIPKKL EAKEISIDIG TAGSITLVLQ 100
    ALLPAMVFAR EKVKFRITGG TDVSWSPPVD YLSNVTLFAL EKIGIHGEIR 150
    VIRRGHYPKG GGIVEGYVEP WNEKRELVAK EYSRIIKIEG ISHATNLPSH 200
    VAERQARAAK DELLQLKVPI EIRTEISRSI GPGSGIVVWA ETDCLRLGGD 250
    ALGKKGKPAE IVGKEAAQEL LDQLKPGHCV DKFLGDQLIP FLAFSGGVIW 300
    VSEITNHLKT NIWVVESFLG RIFDVDGNVG EPGKIRVIRR V 341
    Length:341
    Mass (Da):37,167
    Last modified:December 1, 2000 - v2
    Checksum:i1E24A4F6BF9FAC29
    GO

    Sequence cautioni

    The sequence BAA30639.1 differs from that shown. Reason: Erroneous initiation.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000001 Genomic DNA. Translation: BAA30639.1. Different initiation.
    PIRiG71029.
    RefSeqiNP_143389.1. NC_000961.1.

    Genome annotation databases

    EnsemblBacteriaiBAA30639; BAA30639; BAA30639.
    GeneIDi1443845.
    KEGGipho:PH1529.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000001 Genomic DNA. Translation: BAA30639.1 . Different initiation.
    PIRi G71029.
    RefSeqi NP_143389.1. NC_000961.1.

    3D structure databases

    ProteinModelPortali O59198.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 70601.PH1529.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAA30639 ; BAA30639 ; BAA30639 .
    GeneIDi 1443845.
    KEGGi pho:PH1529.

    Phylogenomic databases

    eggNOGi COG0430.
    HOGENOMi HOG000015264.
    KOi K18105.
    OMAi ERTRTGH.

    Enzyme and pathway databases

    BioCyci PHOR70601:GJWR-1530-MONOMER.

    Family and domain databases

    Gene3Di 3.30.360.20. 1 hit.
    3.65.10.20. 2 hits.
    HAMAPi MF_00200. RTC.
    InterProi IPR013791. RNA3'-term_phos_cycl_insert.
    IPR023797. RNA3'_phos_cyclase_dom.
    IPR000228. RNA3'_term_phos_cyc.
    IPR017770. RNA3'_term_phos_cyc_type_1.
    IPR020719. RNA3'_term_phos_cycl-like_CS.
    IPR013792. RNA3'P_cycl/enolpyr_Trfase_a/b.
    [Graphical view ]
    PANTHERi PTHR11096. PTHR11096. 1 hit.
    Pfami PF01137. RTC. 1 hit.
    PF05189. RTC_insert. 1 hit.
    [Graphical view ]
    SUPFAMi SSF55205. SSF55205. 1 hit.
    TIGRFAMsi TIGR03399. RNA_3prim_cycl. 1 hit.
    PROSITEi PS01287. RTC. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3.

    Entry informationi

    Entry nameiRTCA_PYRHO
    AccessioniPrimary (citable) accession number: O59198
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 15, 1998
    Last sequence update: December 1, 2000
    Last modified: October 1, 2014
    This is version 84 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3