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Protein

Elongation factor 1-alpha

Gene

tuf

Organism
Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.UniRule annotation

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi14 – 21GTPUniRule annotation8
Nucleotide bindingi89 – 93GTPUniRule annotation5
Nucleotide bindingi144 – 147GTPUniRule annotation4

GO - Molecular functioni

Keywordsi

Molecular functionElongation factor
Biological processProtein biosynthesis
LigandGTP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciPHOR70601:G1G39-1440-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Elongation factor 1-alphaUniRule annotation
Short name:
EF-1-alphaUniRule annotation
Alternative name(s):
Elongation factor TuUniRule annotation
Short name:
EF-TuUniRule annotation
Gene namesi
Name:tufUniRule annotation
Ordered Locus Names:PH1484
ORF Names:PHCC033
OrganismiPyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Taxonomic identifieri70601 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
Proteomesi
  • UP000000752 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000909901 – 428Elongation factor 1-alphaAdd BLAST428

Interactioni

Protein-protein interaction databases

STRINGi70601.PH1484

Structurei

Secondary structure

1428
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi7 – 15Combined sources9
Helixi20 – 31Combined sources12
Helixi36 – 42Combined sources7
Helixi54 – 66Combined sources13
Helixi71 – 74Combined sources4
Beta strandi77 – 79Combined sources3
Beta strandi84 – 89Combined sources6
Beta strandi91 – 93Combined sources3
Helixi96 – 102Combined sources7
Beta strandi103 – 105Combined sources3
Beta strandi108 – 115Combined sources8
Turni116 – 118Combined sources3
Helixi122 – 133Combined sources12
Beta strandi138 – 144Combined sources7
Helixi146 – 149Combined sources4
Helixi153 – 170Combined sources18
Beta strandi177 – 179Combined sources3
Turni182 – 184Combined sources3
Turni186 – 188Combined sources3
Helixi203 – 208Combined sources6
Helixi216 – 218Combined sources3
Beta strandi222 – 231Combined sources10
Turni232 – 234Combined sources3
Beta strandi235 – 241Combined sources7
Beta strandi252 – 256Combined sources5
Helixi258 – 262Combined sources5
Beta strandi267 – 275Combined sources9
Beta strandi278 – 283Combined sources6
Beta strandi288 – 293Combined sources6
Helixi298 – 300Combined sources3
Beta strandi306 – 312Combined sources7
Beta strandi322 – 329Combined sources8
Beta strandi343 – 346Combined sources4
Beta strandi349 – 362Combined sources14
Turni364 – 366Combined sources3
Beta strandi369 – 373Combined sources5
Beta strandi382 – 391Combined sources10
Turni398 – 400Combined sources3
Helixi402 – 404Combined sources3
Beta strandi406 – 411Combined sources6
Beta strandi414 – 425Combined sources12

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3WY9X-ray2.30A/B1-428[»]
3WYAX-ray2.35A1-428[»]
ProteinModelPortaliO59153
SMRiO59153
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini5 – 217tr-type GAdd BLAST213

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni14 – 21G1By similarity8
Regioni68 – 72G2By similarity5
Regioni89 – 92G3By similarity4
Regioni144 – 147G4By similarity4
Regioni181 – 183G5By similarity3

Sequence similaritiesi

Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-Tu/EF-1A subfamily.UniRule annotation

Phylogenomic databases

eggNOGiarCOG01561 Archaea
COG5256 LUCA
HOGENOMiHOG000229291
KOiK03231
OMAiAIRDMGM
OrthoDBiPOG093Z025H

Family and domain databases

HAMAPiMF_00118_A EF_Tu_A, 1 hit
InterProiView protein in InterPro
IPR004161 EFTu-like_2
IPR031157 G_TR_CS
IPR027417 P-loop_NTPase
IPR005225 Small_GTP-bd_dom
IPR000795 TF_GTP-bd_dom
IPR009000 Transl_B-barrel_sf
IPR009001 Transl_elong_EF1A/Init_IF2_C
IPR004539 Transl_elong_EF1A_euk/arc
IPR004160 Transl_elong_EFTu/EF1A_C
PfamiView protein in Pfam
PF00009 GTP_EFTU, 1 hit
PF03144 GTP_EFTU_D2, 1 hit
PF03143 GTP_EFTU_D3, 1 hit
PRINTSiPR00315 ELONGATNFCT
SUPFAMiSSF50447 SSF50447, 1 hit
SSF50465 SSF50465, 1 hit
SSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR00483 EF-1_alpha, 1 hit
TIGR00231 small_GTP, 1 hit
PROSITEiView protein in PROSITE
PS00301 G_TR_1, 1 hit
PS51722 G_TR_2, 1 hit

Sequencei

Sequence statusi: Complete.

O59153-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPKEKPHVNI VFIGHVDHGK STTIGRLLYD TGNIPETIIK KFEEMGEKGK
60 70 80 90 100
SFKFAWVMDR LKEERERGIT IDVAHTKFET PHRYITIIDA PGHRDFVKNM
110 120 130 140 150
ITGASQADAA VLVVAATDGV MPQTKEHAFL ARTLGIKHII VTINKMDMVN
160 170 180 190 200
YDQKVFEKVK AQVEKLLKTL GYKDFPVIPT SAWNGDNVVK KSDKMPWYNG
210 220 230 240 250
PTLIEALDQI PEPEKPIDKP LRIPIQDVYS IKGVGTVPVG RVETGKLKVG
260 270 280 290 300
DVVIFEPAST IFHKPIQGEV KSIEMHHEPL QEALPGDNIG FNVRGVSKND
310 320 330 340 350
IKRGDVAGHT DKPPTVVRTK DTFKAQIIVL NHPTAITVGY SPVLHAHTAQ
360 370 380 390 400
IPVRFEQILA KVDPRTGNIV EENPQFIKTG DSAIVVLRPM KPVVLEPVKE
410 420
IPQLGRFAIR DMGMTIAAGM VISIQKGE
Length:428
Mass (Da):47,525
Last modified:August 1, 1998 - v1
Checksum:i830379915E67E6EA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000001 Genomic DNA Translation: BAA30591.1
PIRiG71023
RefSeqiWP_010885563.1, NC_000961.1

Genome annotation databases

EnsemblBacteriaiBAA30591; BAA30591; BAA30591
GeneIDi1443801
KEGGipho:PH1484

Similar proteinsi

Entry informationi

Entry nameiEF1A_PYRHO
AccessioniPrimary (citable) accession number: O59153
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: August 1, 1998
Last modified: April 25, 2018
This is version 113 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

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