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Protein

Elongation factor 1-alpha

Gene

tuf

Organism
Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.UniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi14 – 218GTPUniRule annotation
Nucleotide bindingi89 – 935GTPUniRule annotation
Nucleotide bindingi144 – 1474GTPUniRule annotation

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Elongation factor

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciPHOR70601:GJWR-1483-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Elongation factor 1-alphaUniRule annotation
Short name:
EF-1-alphaUniRule annotation
Alternative name(s):
Elongation factor TuUniRule annotation
Short name:
EF-TuUniRule annotation
Gene namesi
Name:tufUniRule annotation
Ordered Locus Names:PH1484
ORF Names:PHCC033
OrganismiPyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Taxonomic identifieri70601 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
Proteomesi
  • UP000000752 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 428428Elongation factor 1-alphaPRO_0000090990Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi70601.PH1484.

Structurei

Secondary structure

1
428
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi7 – 159Combined sources
Helixi20 – 3112Combined sources
Helixi36 – 427Combined sources
Helixi54 – 6613Combined sources
Helixi71 – 744Combined sources
Beta strandi77 – 793Combined sources
Beta strandi84 – 896Combined sources
Beta strandi91 – 933Combined sources
Helixi96 – 1027Combined sources
Beta strandi103 – 1053Combined sources
Beta strandi108 – 1158Combined sources
Turni116 – 1183Combined sources
Helixi122 – 13312Combined sources
Beta strandi138 – 1447Combined sources
Helixi146 – 1494Combined sources
Helixi153 – 17018Combined sources
Beta strandi177 – 1793Combined sources
Turni182 – 1843Combined sources
Turni186 – 1883Combined sources
Helixi203 – 2086Combined sources
Helixi216 – 2183Combined sources
Beta strandi222 – 23110Combined sources
Turni232 – 2343Combined sources
Beta strandi235 – 2417Combined sources
Beta strandi252 – 2565Combined sources
Helixi258 – 2625Combined sources
Beta strandi267 – 2759Combined sources
Beta strandi278 – 2836Combined sources
Beta strandi288 – 2936Combined sources
Helixi298 – 3003Combined sources
Beta strandi306 – 3127Combined sources
Beta strandi322 – 3298Combined sources
Beta strandi343 – 3464Combined sources
Beta strandi349 – 36214Combined sources
Turni364 – 3663Combined sources
Beta strandi369 – 3735Combined sources
Beta strandi382 – 39110Combined sources
Turni398 – 4003Combined sources
Helixi402 – 4043Combined sources
Beta strandi406 – 4116Combined sources
Beta strandi414 – 42512Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3WY9X-ray2.30A/B1-428[»]
3WYAX-ray2.35A1-428[»]
ProteinModelPortaliO59153.
SMRiO59153. Positions 5-425.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini5 – 217213tr-type GAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni14 – 218G1By similarity
Regioni68 – 725G2By similarity
Regioni89 – 924G3By similarity
Regioni144 – 1474G4By similarity
Regioni181 – 1833G5By similarity

Sequence similaritiesi

Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-Tu/EF-1A subfamily.UniRule annotation

Phylogenomic databases

eggNOGiarCOG01561. Archaea.
COG5256. LUCA.
HOGENOMiHOG000229291.
KOiK03231.
OMAiPASTIFH.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00118_A. EF_Tu_A.
InterProiIPR004161. EFTu-like_2.
IPR031157. G_TR_CS.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR000795. TF_GTP-bd_dom.
IPR009000. Transl_B-barrel.
IPR009001. Transl_elong_EF1A/Init_IF2_C.
IPR004539. Transl_elong_EF1A_euk/arc.
IPR004160. Transl_elong_EFTu/EF1A_C.
[Graphical view]
PfamiPF03144. GTP_EFTU_D2. 1 hit.
PF03143. GTP_EFTU_D3. 1 hit.
[Graphical view]
PRINTSiPR00315. ELONGATNFCT.
SUPFAMiSSF50447. SSF50447. 1 hit.
SSF50465. SSF50465. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00483. EF-1_alpha. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEiPS00301. G_TR_1. 1 hit.
PS51722. G_TR_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O59153-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPKEKPHVNI VFIGHVDHGK STTIGRLLYD TGNIPETIIK KFEEMGEKGK
60 70 80 90 100
SFKFAWVMDR LKEERERGIT IDVAHTKFET PHRYITIIDA PGHRDFVKNM
110 120 130 140 150
ITGASQADAA VLVVAATDGV MPQTKEHAFL ARTLGIKHII VTINKMDMVN
160 170 180 190 200
YDQKVFEKVK AQVEKLLKTL GYKDFPVIPT SAWNGDNVVK KSDKMPWYNG
210 220 230 240 250
PTLIEALDQI PEPEKPIDKP LRIPIQDVYS IKGVGTVPVG RVETGKLKVG
260 270 280 290 300
DVVIFEPAST IFHKPIQGEV KSIEMHHEPL QEALPGDNIG FNVRGVSKND
310 320 330 340 350
IKRGDVAGHT DKPPTVVRTK DTFKAQIIVL NHPTAITVGY SPVLHAHTAQ
360 370 380 390 400
IPVRFEQILA KVDPRTGNIV EENPQFIKTG DSAIVVLRPM KPVVLEPVKE
410 420
IPQLGRFAIR DMGMTIAAGM VISIQKGE
Length:428
Mass (Da):47,525
Last modified:August 1, 1998 - v1
Checksum:i830379915E67E6EA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000001 Genomic DNA. Translation: BAA30591.1.
PIRiG71023.
RefSeqiWP_010885563.1. NC_000961.1.

Genome annotation databases

EnsemblBacteriaiBAA30591; BAA30591; BAA30591.
GeneIDi1443801.
KEGGipho:PH1484.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000001 Genomic DNA. Translation: BAA30591.1.
PIRiG71023.
RefSeqiWP_010885563.1. NC_000961.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3WY9X-ray2.30A/B1-428[»]
3WYAX-ray2.35A1-428[»]
ProteinModelPortaliO59153.
SMRiO59153. Positions 5-425.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi70601.PH1484.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAA30591; BAA30591; BAA30591.
GeneIDi1443801.
KEGGipho:PH1484.

Phylogenomic databases

eggNOGiarCOG01561. Archaea.
COG5256. LUCA.
HOGENOMiHOG000229291.
KOiK03231.
OMAiPASTIFH.

Enzyme and pathway databases

BioCyciPHOR70601:GJWR-1483-MONOMER.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00118_A. EF_Tu_A.
InterProiIPR004161. EFTu-like_2.
IPR031157. G_TR_CS.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR000795. TF_GTP-bd_dom.
IPR009000. Transl_B-barrel.
IPR009001. Transl_elong_EF1A/Init_IF2_C.
IPR004539. Transl_elong_EF1A_euk/arc.
IPR004160. Transl_elong_EFTu/EF1A_C.
[Graphical view]
PfamiPF03144. GTP_EFTU_D2. 1 hit.
PF03143. GTP_EFTU_D3. 1 hit.
[Graphical view]
PRINTSiPR00315. ELONGATNFCT.
SUPFAMiSSF50447. SSF50447. 1 hit.
SSF50465. SSF50465. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00483. EF-1_alpha. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEiPS00301. G_TR_1. 1 hit.
PS51722. G_TR_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3.

Entry informationi

Entry nameiEF1A_PYRHO
AccessioniPrimary (citable) accession number: O59153
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: August 1, 1998
Last modified: July 6, 2016
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.