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O59150

- RNP2_PYRHO

UniProt

O59150 - RNP2_PYRHO

Protein

Ribonuclease P protein component 2

Gene

rnp2

Organism
Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 78 (01 Oct 2014)
      Sequence version 1 (01 Aug 1998)
      Previous versions | rss
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    Functioni

    Part of ribonuclease P, a protein complex that generates mature tRNA molecules by cleaving their 5'-ends.3 PublicationsUniRule annotation

    Catalytic activityi

    Endonucleolytic cleavage of RNA, removing 5'-extranucleotides from tRNA precursor.UniRule annotation

    Temperature dependencei

    Optimum temperature is 70 degrees Celsius.2 Publications

    GO - Molecular functioni

    1. protein binding Source: IntAct
    2. ribonuclease P activity Source: UniProtKB

    GO - Biological processi

    1. RNA phosphodiester bond hydrolysis Source: GOC
    2. RNA phosphodiester bond hydrolysis, endonucleolytic Source: GOC
    3. tRNA 5'-leader removal Source: UniProtKB

    Keywords - Molecular functioni

    Endonuclease, Hydrolase, Nuclease

    Keywords - Biological processi

    tRNA processing

    Enzyme and pathway databases

    BioCyciPHOR70601:GJWR-1479-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ribonuclease P protein component 2UniRule annotation (EC:3.1.26.5UniRule annotation)
    Short name:
    RNase P component 2UniRule annotation
    Alternative name(s):
    Pop5UniRule annotation
    Gene namesi
    Name:rnp2UniRule annotation
    Ordered Locus Names:PH1481
    OrganismiPyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
    Taxonomic identifieri70601 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
    ProteomesiUP000000752: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. ribonuclease P complex Source: UniProtKB

    Keywords - Cellular componenti

    Cytoplasm

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi43 – 486Missing: Froms heterodimer with Rnp3, but not heterotetramer. Does not reconstitute RNase P activity.
    Mutagenesisi72 – 721C → S: Fully reconstitutes RNase P activity. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 120120Ribonuclease P protein component 2PRO_0000140027Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer in solution. Component of RNase P which consists of a catalytic RNA component and at least 5 protein subunits. Forms a heterotetrameric subcomplex with Rnp3. Reconstituted enzyme missing individual protein subunits is suboptimally active, showing each subunit contributes to optimization of activity.4 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    rnp3O595432EBI-2603177,EBI-2603192
    rnp4O592483EBI-2603177,EBI-2641275

    Protein-protein interaction databases

    IntActiO59150. 2 interactions.
    STRINGi70601.PH1481.

    Structurei

    Secondary structure

    1
    120
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Turni10 – 123
    Beta strandi16 – 249
    Helixi31 – 5424
    Beta strandi57 – 626
    Turni63 – 664
    Beta strandi67 – 737
    Helixi74 – 763
    Helixi77 – 859
    Beta strandi96 – 10510
    Helixi106 – 1138
    Helixi115 – 1173

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2CZVX-ray2.00C/D1-120[»]
    ProteinModelPortaliO59150.
    SMRiO59150. Positions 2-120.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiO59150.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the eukaryotic/archaeal RNase P protein component 2 family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG1369.
    HOGENOMiHOG000286243.
    KOiK03537.
    OMAiNPWLIDY.

    Family and domain databases

    HAMAPiMF_00755. RNase_P_2.
    InterProiIPR002759. RNase_P/MRP_subunit.
    IPR016434. RNase_P_comp-2.
    [Graphical view]
    PfamiPF01900. RNase_P_Rpp14. 1 hit.
    [Graphical view]
    PIRSFiPIRSF004952. RNase_P_2. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    O59150-1 [UniParc]FASTAAdd to Basket

    « Hide

    MMRKLKTLPP TLRDKNRYIA FEIISDGDFT KDEVKELIWK SSLEVLGETG    50
    TAIVKPWLIK FDPNTKTGIV RCDREYVEYL RFALMLVSEF NGKRLIIRTL 100
    GVSGTIKRLK RKFLAKYGWK 120
    Length:120
    Mass (Da):14,044
    Last modified:August 1, 1998 - v1
    Checksum:iC78C9EF6C5380E53
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000001 Genomic DNA. Translation: BAA30588.1.
    PIRiD71023.
    RefSeqiNP_143344.1. NC_000961.1.

    Genome annotation databases

    EnsemblBacteriaiBAA30588; BAA30588; BAA30588.
    GeneIDi1443798.
    KEGGipho:PH1481.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000001 Genomic DNA. Translation: BAA30588.1 .
    PIRi D71023.
    RefSeqi NP_143344.1. NC_000961.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2CZV X-ray 2.00 C/D 1-120 [» ]
    ProteinModelPortali O59150.
    SMRi O59150. Positions 2-120.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi O59150. 2 interactions.
    STRINGi 70601.PH1481.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAA30588 ; BAA30588 ; BAA30588 .
    GeneIDi 1443798.
    KEGGi pho:PH1481.

    Phylogenomic databases

    eggNOGi COG1369.
    HOGENOMi HOG000286243.
    KOi K03537.
    OMAi NPWLIDY.

    Enzyme and pathway databases

    BioCyci PHOR70601:GJWR-1479-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei O59150.

    Family and domain databases

    HAMAPi MF_00755. RNase_P_2.
    InterProi IPR002759. RNase_P/MRP_subunit.
    IPR016434. RNase_P_comp-2.
    [Graphical view ]
    Pfami PF01900. RNase_P_Rpp14. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF004952. RNase_P_2. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3.
    2. "Reconstitution of archaeal ribonuclease P from RNA and four protein components."
      Kouzuma Y., Mizoguchi M., Takagi H., Fukuhara H., Tsukamoto M., Numata T., Kimura M.
      Biochem. Biophys. Res. Commun. 306:666-673(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, SUBUNIT.
      Strain: ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3.
    3. "A fifth protein subunit Ph1496p elevates the optimum temperature for the ribonuclease P activity from Pyrococcus horikoshii OT3."
      Fukuhara H., Kifusa M., Watanabe M., Terada A., Honda T., Numata T., Kakuta Y., Kimura M.
      Biochem. Biophys. Res. Commun. 343:956-964(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, SUBUNIT.
      Strain: ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3.
    4. "Characterization of the archaeal ribonuclease P proteins from Pyrococcus horikoshii OT3."
      Terada A., Honda T., Fukuhara H., Hada K., Kimura M.
      J. Biochem. 140:293-298(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBUNIT.
      Strain: ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3.
    5. "Crystal structure of protein Ph1481p in complex with protein Ph1877p of archaeal RNase P from Pyrococcus horikoshii OT3: implication of dimer formation of the holoenzyme."
      Kawano S., Nakashima T., Kakuta Y., Tanaka I., Kimura M.
      J. Mol. Biol. 357:583-591(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) IN COMPLEX WITH RNP3, SUBUNIT, MUTAGENESIS OF 43-LEU--GLU-48 AND CYS-72.
      Strain: ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3.

    Entry informationi

    Entry nameiRNP2_PYRHO
    AccessioniPrimary (citable) accession number: O59150
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 13, 2004
    Last sequence update: August 1, 1998
    Last modified: October 1, 2014
    This is version 78 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3