Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Glutamyl-tRNA(Gln) amidotransferase subunit D

Gene

gatD

Organism
Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln). The GatDE system is specific for glutamate and does not act on aspartate.UniRule annotation

Catalytic activityi

ATP + L-glutamyl-tRNA(Gln) + L-glutamine = ADP + phosphate + L-glutaminyl-tRNA(Gln) + L-glutamate.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei102UniRule annotation1
Active sitei178UniRule annotation1
Active sitei179UniRule annotation1
Active sitei256UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
Biological processProtein biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciPHOR70601:G1G39-1420-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamyl-tRNA(Gln) amidotransferase subunit DUniRule annotation (EC:6.3.5.-UniRule annotation)
Short name:
Glu-ADT subunit DUniRule annotation
Gene namesi
Name:gatDUniRule annotation
Ordered Locus Names:PH1463
OrganismiPyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Taxonomic identifieri70601 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
Proteomesi
  • UP000000752 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001400611 – 438Glutamyl-tRNA(Gln) amidotransferase subunit DAdd BLAST438

Interactioni

Subunit structurei

Heterodimer of GatD and GatE.UniRule annotation

Protein-protein interaction databases

STRINGi70601.PH1463

Structurei

3D structure databases

ProteinModelPortaliO59132
SMRiO59132
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini92 – 422Asparaginase/glutaminasePROSITE-ProRule annotationAdd BLAST331

Sequence similaritiesi

Belongs to the asparaginase 1 family. GatD subfamily.UniRule annotation

Phylogenomic databases

eggNOGiarCOG01924 Archaea
COG0252 LUCA
HOGENOMiHOG000227975
KOiK09482
OMAiNVYSTGR
OrthoDBiPOG093Z07VD

Family and domain databases

CDDicd08962 GatD, 1 hit
Gene3Di2.30.30.520, 1 hit
3.40.50.1170, 1 hit
3.40.50.40, 1 hit
HAMAPiMF_00586 GatD, 1 hit
InterProiView protein in InterPro
IPR006033 AsnASEI
IPR036152 Asp/glu_Ase-like_sf
IPR006034 Asparaginase/glutaminase-like
IPR020827 Asparaginase/glutaminase_AS1
IPR027475 Asparaginase/glutaminase_AS2
IPR011878 GatD
IPR037222 GatD_N
IPR027473 L-asparaginase_C
IPR027474 L-asparaginase_N
IPR037152 L-asparaginase_N_sf
PfamiView protein in Pfam
PF00710 Asparaginase, 1 hit
PIRSFiPIRSF500175 Glu_ADT_D, 1 hit
PIRSF001220 L-ASNase_gatD, 1 hit
PRINTSiPR00139 ASNGLNASE
SMARTiView protein in SMART
SM00870 Asparaginase, 1 hit
SUPFAMiSSF141300 SSF141300, 1 hit
SSF53774 SSF53774, 1 hit
TIGRFAMsiTIGR00519 asnASE_I, 1 hit
TIGR02153 gatD_arch, 1 hit
PROSITEiView protein in PROSITE
PS00144 ASN_GLN_ASE_1, 1 hit
PS00917 ASN_GLN_ASE_2, 1 hit
PS51732 ASN_GLN_ASE_3, 1 hit

Sequencei

Sequence statusi: Complete.

O59132-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRVEEFLKEK NINIGDFIRV IKEEDGEEVI YEGYVMPPYE LSPGDTLVLK
60 70 80 90 100
LENGYNIGIA LSKIRRVEVI ERAKVKPEIH FEAFIEGKPH LPDVTIIGTG
110 120 130 140 150
GTIASRIDYE TGAVYPAFTA EELAKAVPEI FEIANIKPKL LFNIFSEDMK
160 170 180 190 200
PKHWIKIAHE VAKSLNSGDS GVVVAHGTDT MGYTAAALSF MLRDLGKPVI
210 220 230 240 250
LVGAQRSSDR PSSDAAMNLI CSVRMSTSDV AEVMVVMHGE TGDTYCLAHR
260 270 280 290 300
GTKVRKMHTS RRDAFRSIND VPIAKVWPNG KIEFLRDDYR RRSDSEVWVD
310 320 330 340 350
DKLEEKVALV KVYPGISSEI IEFFIDKGYR GIVIEGTGLG HTPNDIIPSI
360 370 380 390 400
QRATEEGIAV CMTSQCIYGR VNLNVYATGR RLLKAGVIPC EDMLPETAYV
410 420 430
KLMWVLGHTQ DLEEVRRMML TNYAGEITPY TRFDTYLR
Length:438
Mass (Da):49,115
Last modified:August 1, 1998 - v1
Checksum:i50A2FC5F377E8FBF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000001 Genomic DNA Translation: BAA30570.1
PIRiB71021
RefSeqiWP_010885545.1, NC_000961.1

Genome annotation databases

EnsemblBacteriaiBAA30570; BAA30570; BAA30570
GeneIDi1443783
KEGGipho:PH1463

Similar proteinsi

Entry informationi

Entry nameiGATD_PYRHO
AccessioniPrimary (citable) accession number: O59132
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 25, 2002
Last sequence update: August 1, 1998
Last modified: April 25, 2018
This is version 99 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health