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Protein

Glutamyl-tRNA(Gln) amidotransferase subunit D

Gene

gatD

Organism
Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln). The GatDE system is specific for glutamate and does not act on aspartate.UniRule annotation

Catalytic activityi

ATP + L-glutamyl-tRNA(Gln) + L-glutamine = ADP + phosphate + L-glutaminyl-tRNA(Gln) + L-glutamate.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei102 – 1021UniRule annotation
Active sitei178 – 1781UniRule annotation
Active sitei179 – 1791UniRule annotation
Active sitei256 – 2561UniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciPHOR70601:GJWR-1463-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamyl-tRNA(Gln) amidotransferase subunit DUniRule annotation (EC:6.3.5.-UniRule annotation)
Short name:
Glu-ADT subunit DUniRule annotation
Gene namesi
Name:gatDUniRule annotation
Ordered Locus Names:PH1463
OrganismiPyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Taxonomic identifieri70601 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
Proteomesi
  • UP000000752 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 438438Glutamyl-tRNA(Gln) amidotransferase subunit DPRO_0000140061Add
BLAST

Interactioni

Subunit structurei

Heterodimer of GatD and GatE.UniRule annotation

Protein-protein interaction databases

STRINGi70601.PH1463.

Structurei

3D structure databases

ProteinModelPortaliO59132.
SMRiO59132. Positions 2-438.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini92 – 422331Asparaginase/glutaminasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the asparaginase 1 family. GatD subfamily.UniRule annotation
Contains 1 asparaginase/glutaminase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiarCOG01924. Archaea.
COG0252. LUCA.
HOGENOMiHOG000227975.
KOiK09482.
OMAiMHGETGD.

Family and domain databases

CDDicd08962. GatD. 1 hit.
Gene3Di3.40.50.1170. 1 hit.
3.40.50.40. 1 hit.
HAMAPiMF_00586. GatD. 1 hit.
InterProiIPR006033. AsnASEI.
IPR006034. Asparaginase/glutaminase.
IPR020827. Asparaginase/glutaminase_AS1.
IPR027475. Asparaginase/glutaminase_AS2.
IPR011878. GatD.
IPR027473. L-asparaginase_C.
IPR027474. L-asparaginase_N.
[Graphical view]
PfamiPF00710. Asparaginase. 1 hit.
[Graphical view]
PIRSFiPIRSF500175. Glu_ADT_D. 1 hit.
PIRSF001220. L-ASNase_gatD. 1 hit.
PRINTSiPR00139. ASNGLNASE.
SMARTiSM00870. Asparaginase. 1 hit.
[Graphical view]
SUPFAMiSSF53774. SSF53774. 1 hit.
TIGRFAMsiTIGR00519. asnASE_I. 1 hit.
TIGR02153. gatD_arch. 1 hit.
PROSITEiPS00144. ASN_GLN_ASE_1. 1 hit.
PS00917. ASN_GLN_ASE_2. 1 hit.
PS51732. ASN_GLN_ASE_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O59132-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRVEEFLKEK NINIGDFIRV IKEEDGEEVI YEGYVMPPYE LSPGDTLVLK
60 70 80 90 100
LENGYNIGIA LSKIRRVEVI ERAKVKPEIH FEAFIEGKPH LPDVTIIGTG
110 120 130 140 150
GTIASRIDYE TGAVYPAFTA EELAKAVPEI FEIANIKPKL LFNIFSEDMK
160 170 180 190 200
PKHWIKIAHE VAKSLNSGDS GVVVAHGTDT MGYTAAALSF MLRDLGKPVI
210 220 230 240 250
LVGAQRSSDR PSSDAAMNLI CSVRMSTSDV AEVMVVMHGE TGDTYCLAHR
260 270 280 290 300
GTKVRKMHTS RRDAFRSIND VPIAKVWPNG KIEFLRDDYR RRSDSEVWVD
310 320 330 340 350
DKLEEKVALV KVYPGISSEI IEFFIDKGYR GIVIEGTGLG HTPNDIIPSI
360 370 380 390 400
QRATEEGIAV CMTSQCIYGR VNLNVYATGR RLLKAGVIPC EDMLPETAYV
410 420 430
KLMWVLGHTQ DLEEVRRMML TNYAGEITPY TRFDTYLR
Length:438
Mass (Da):49,115
Last modified:August 1, 1998 - v1
Checksum:i50A2FC5F377E8FBF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000001 Genomic DNA. Translation: BAA30570.1.
PIRiB71021.
RefSeqiWP_010885545.1. NC_000961.1.

Genome annotation databases

EnsemblBacteriaiBAA30570; BAA30570; BAA30570.
GeneIDi1443783.
KEGGipho:PH1463.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000001 Genomic DNA. Translation: BAA30570.1.
PIRiB71021.
RefSeqiWP_010885545.1. NC_000961.1.

3D structure databases

ProteinModelPortaliO59132.
SMRiO59132. Positions 2-438.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi70601.PH1463.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAA30570; BAA30570; BAA30570.
GeneIDi1443783.
KEGGipho:PH1463.

Phylogenomic databases

eggNOGiarCOG01924. Archaea.
COG0252. LUCA.
HOGENOMiHOG000227975.
KOiK09482.
OMAiMHGETGD.

Enzyme and pathway databases

BioCyciPHOR70601:GJWR-1463-MONOMER.

Family and domain databases

CDDicd08962. GatD. 1 hit.
Gene3Di3.40.50.1170. 1 hit.
3.40.50.40. 1 hit.
HAMAPiMF_00586. GatD. 1 hit.
InterProiIPR006033. AsnASEI.
IPR006034. Asparaginase/glutaminase.
IPR020827. Asparaginase/glutaminase_AS1.
IPR027475. Asparaginase/glutaminase_AS2.
IPR011878. GatD.
IPR027473. L-asparaginase_C.
IPR027474. L-asparaginase_N.
[Graphical view]
PfamiPF00710. Asparaginase. 1 hit.
[Graphical view]
PIRSFiPIRSF500175. Glu_ADT_D. 1 hit.
PIRSF001220. L-ASNase_gatD. 1 hit.
PRINTSiPR00139. ASNGLNASE.
SMARTiSM00870. Asparaginase. 1 hit.
[Graphical view]
SUPFAMiSSF53774. SSF53774. 1 hit.
TIGRFAMsiTIGR00519. asnASE_I. 1 hit.
TIGR02153. gatD_arch. 1 hit.
PROSITEiPS00144. ASN_GLN_ASE_1. 1 hit.
PS00917. ASN_GLN_ASE_2. 1 hit.
PS51732. ASN_GLN_ASE_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGATD_PYRHO
AccessioniPrimary (citable) accession number: O59132
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 25, 2002
Last sequence update: August 1, 1998
Last modified: September 7, 2016
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.