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Protein

GMP synthase [glutamine-hydrolyzing] subunit B

Gene

guaAB

Organism
Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the synthesis of GMP from XMP.By similarity

Catalytic activityi

ATP + XMP + L-glutamine + H2O = AMP + diphosphate + GMP + L-glutamate.

Pathwayi: GMP biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes GMP from XMP (L-Gln route).
Proteins known to be involved in this subpathway in this organism are:
  1. GMP synthase [glutamine-hydrolyzing] subunit A (guaAA), GMP synthase [glutamine-hydrolyzing] subunit B (guaAB)
This subpathway is part of the pathway GMP biosynthesis, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes GMP from XMP (L-Gln route), the pathway GMP biosynthesis and in Purine metabolism.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi28 – 34ATPBy similarity7

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

GMP biosynthesis, Purine biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi6.3.5.2. 5244.
UniPathwayiUPA00189; UER00296.

Names & Taxonomyi

Protein namesi
Recommended name:
GMP synthase [glutamine-hydrolyzing] subunit B (EC:6.3.5.2)
Alternative name(s):
GMP synthetase
Gene namesi
Name:guaAB
Ordered Locus Names:PH1347
OrganismiPyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Taxonomic identifieri70601 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
Proteomesi
  • UP000000752 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001402491 – 308GMP synthase [glutamine-hydrolyzing] subunit BAdd BLAST308

Interactioni

Subunit structurei

Heterodimer composed of a glutamine amidotransferase subunit (A) and a GMP-binding subunit (B).Curated

Protein-protein interaction databases

STRINGi70601.PH1347.

Structurei

Secondary structure

1308
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi3 – 18Combined sources16
Beta strandi23 – 26Combined sources4
Helixi31 – 44Combined sources14
Helixi45 – 47Combined sources3
Beta strandi48 – 54Combined sources7
Helixi62 – 70Combined sources9
Turni71 – 74Combined sources4
Beta strandi77 – 82Combined sources6
Helixi84 – 90Combined sources7
Turni91 – 93Combined sources3
Helixi97 – 119Combined sources23
Beta strandi122 – 125Combined sources4
Beta strandi156 – 158Combined sources3
Turni160 – 163Combined sources4
Helixi166 – 175Combined sources10
Helixi180 – 183Combined sources4
Helixi192 – 196Combined sources5
Beta strandi197 – 200Combined sources4
Helixi203 – 222Combined sources20
Beta strandi228 – 234Combined sources7
Beta strandi239 – 259Combined sources21
Beta strandi261 – 268Combined sources8
Helixi273 – 286Combined sources14
Beta strandi290 – 296Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2DPLX-ray1.43A/B1-308[»]
3A4IX-ray1.79A/B1-308[»]
ProteinModelPortaliO59072.
SMRiO59072.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO59072.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 185GMPS ATP-PPaseAdd BLAST185

Sequence similaritiesi

Phylogenomic databases

eggNOGiarCOG00085. Archaea.
COG0519. LUCA.
HOGENOMiHOG000223964.
KOiK01951.
OMAiGGIKSHH.

Family and domain databases

CDDicd01997. GMP_synthase_C. 1 hit.
Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_00345. GMP_synthase_B. 1 hit.
InterProiIPR001674. GMP_synth_C.
IPR026598. GMP_synthase_B.
IPR025777. GMPS_ATP_PPase_dom.
IPR022310. NAD/GMP_synthase.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00958. GMP_synt_C. 1 hit.
PF02540. NAD_synthase. 2 hits.
[Graphical view]
TIGRFAMsiTIGR00884. guaA_Cterm. 1 hit.
PROSITEiPS51553. GMPS_ATP_PPASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O59072-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDWGRFVEEK VREIRETVGD SKAIIALSGG VDSSTAAVLA HKAIGDRLHA
60 70 80 90 100
VFVNTGFLRK GEPEFVVKTF RDEFGMNLHY VDAQDRFFSA LKGVTDPEEK
110 120 130 140 150
RKIIGRVFIE VFEEVAKKIG AEYLIQGTIA PDWIESQGKI KSHHNVGGLP
160 170 180 190 200
EKLNLKLIEP LRDLYKDEVR ELAKFLGLPE KIYNRMPFPG PGLAVRVIGE
210 220 230 240 250
VTPEKIRIVR EANAIVEEEV ERAGLRPWQA FAVLLGVKTV GVQGDIRAYK
260 270 280 290 300
ETIAVRIVES IDGMTANAMN VPWEVLQRIA FRITSEIPEV GRVLYDITNK

PPATIEFE
Length:308
Mass (Da):34,562
Last modified:August 1, 1998 - v1
Checksum:i8B209770605AE4BA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000001 Genomic DNA. Translation: BAA30453.1.
PIRiE71006.
RefSeqiWP_010885436.1. NC_000961.1.

Genome annotation databases

EnsemblBacteriaiBAA30453; BAA30453; BAA30453.
GeneIDi1443673.
KEGGipho:PH1347.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000001 Genomic DNA. Translation: BAA30453.1.
PIRiE71006.
RefSeqiWP_010885436.1. NC_000961.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2DPLX-ray1.43A/B1-308[»]
3A4IX-ray1.79A/B1-308[»]
ProteinModelPortaliO59072.
SMRiO59072.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi70601.PH1347.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAA30453; BAA30453; BAA30453.
GeneIDi1443673.
KEGGipho:PH1347.

Phylogenomic databases

eggNOGiarCOG00085. Archaea.
COG0519. LUCA.
HOGENOMiHOG000223964.
KOiK01951.
OMAiGGIKSHH.

Enzyme and pathway databases

UniPathwayiUPA00189; UER00296.
BRENDAi6.3.5.2. 5244.

Miscellaneous databases

EvolutionaryTraceiO59072.

Family and domain databases

CDDicd01997. GMP_synthase_C. 1 hit.
Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_00345. GMP_synthase_B. 1 hit.
InterProiIPR001674. GMP_synth_C.
IPR026598. GMP_synthase_B.
IPR025777. GMPS_ATP_PPase_dom.
IPR022310. NAD/GMP_synthase.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00958. GMP_synt_C. 1 hit.
PF02540. NAD_synthase. 2 hits.
[Graphical view]
TIGRFAMsiTIGR00884. guaA_Cterm. 1 hit.
PROSITEiPS51553. GMPS_ATP_PPASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGUAAB_PYRHO
AccessioniPrimary (citable) accession number: O59072
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: August 1, 1998
Last modified: November 2, 2016
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.