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Protein

GMP synthase [glutamine-hydrolyzing] subunit B

Gene

guaAB

Organism
Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the synthesis of GMP from XMP.By similarity

Catalytic activityi

ATP + XMP + L-glutamine + H2O = AMP + diphosphate + GMP + L-glutamate.

Pathwayi: GMP biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes GMP from XMP (L-Gln route).
Proteins known to be involved in this subpathway in this organism are:
  1. GMP synthase [glutamine-hydrolyzing] subunit A (guaAA), GMP synthase [glutamine-hydrolyzing] subunit B (guaAB)
This subpathway is part of the pathway GMP biosynthesis, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes GMP from XMP (L-Gln route), the pathway GMP biosynthesis and in Purine metabolism.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi28 – 347ATPBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

GMP biosynthesis, Purine biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciPHOR70601:GJWR-1354-MONOMER.
BRENDAi6.3.5.2. 5244.
UniPathwayiUPA00189; UER00296.

Names & Taxonomyi

Protein namesi
Recommended name:
GMP synthase [glutamine-hydrolyzing] subunit B (EC:6.3.5.2)
Alternative name(s):
GMP synthetase
Gene namesi
Name:guaAB
Ordered Locus Names:PH1347
OrganismiPyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Taxonomic identifieri70601 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
Proteomesi
  • UP000000752 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 308308GMP synthase [glutamine-hydrolyzing] subunit BPRO_0000140249Add
BLAST

Interactioni

Subunit structurei

Heterodimer composed of a glutamine amidotransferase subunit (A) and a GMP-binding subunit (B).Curated

Protein-protein interaction databases

STRINGi70601.PH1347.

Structurei

Secondary structure

1
308
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi3 – 1816Combined sources
Beta strandi23 – 264Combined sources
Helixi31 – 4414Combined sources
Helixi45 – 473Combined sources
Beta strandi48 – 547Combined sources
Helixi62 – 709Combined sources
Turni71 – 744Combined sources
Beta strandi77 – 826Combined sources
Helixi84 – 907Combined sources
Turni91 – 933Combined sources
Helixi97 – 11923Combined sources
Beta strandi122 – 1254Combined sources
Beta strandi156 – 1583Combined sources
Turni160 – 1634Combined sources
Helixi166 – 17510Combined sources
Helixi180 – 1834Combined sources
Helixi192 – 1965Combined sources
Beta strandi197 – 2004Combined sources
Helixi203 – 22220Combined sources
Beta strandi228 – 2347Combined sources
Beta strandi239 – 25921Combined sources
Beta strandi261 – 2688Combined sources
Helixi273 – 28614Combined sources
Beta strandi290 – 2967Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2DPLX-ray1.43A/B1-308[»]
3A4IX-ray1.79A/B1-308[»]
ProteinModelPortaliO59072.
SMRiO59072. Positions 1-308.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO59072.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 185185GMPS ATP-PPaseAdd
BLAST

Sequence similaritiesi

Phylogenomic databases

eggNOGiarCOG00085. Archaea.
COG0519. LUCA.
HOGENOMiHOG000223964.
KOiK01951.
OMAiGGIKSHH.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_00345. GMP_synthase_B. 1 hit.
InterProiIPR001674. GMP_synth_C.
IPR026598. GMP_synthase_B.
IPR025777. GMPS_ATP_PPase_dom.
IPR022310. NAD/GMP_synthase.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00958. GMP_synt_C. 1 hit.
PF02540. NAD_synthase. 2 hits.
[Graphical view]
TIGRFAMsiTIGR00884. guaA_Cterm. 1 hit.
PROSITEiPS51553. GMPS_ATP_PPASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O59072-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDWGRFVEEK VREIRETVGD SKAIIALSGG VDSSTAAVLA HKAIGDRLHA
60 70 80 90 100
VFVNTGFLRK GEPEFVVKTF RDEFGMNLHY VDAQDRFFSA LKGVTDPEEK
110 120 130 140 150
RKIIGRVFIE VFEEVAKKIG AEYLIQGTIA PDWIESQGKI KSHHNVGGLP
160 170 180 190 200
EKLNLKLIEP LRDLYKDEVR ELAKFLGLPE KIYNRMPFPG PGLAVRVIGE
210 220 230 240 250
VTPEKIRIVR EANAIVEEEV ERAGLRPWQA FAVLLGVKTV GVQGDIRAYK
260 270 280 290 300
ETIAVRIVES IDGMTANAMN VPWEVLQRIA FRITSEIPEV GRVLYDITNK

PPATIEFE
Length:308
Mass (Da):34,562
Last modified:August 1, 1998 - v1
Checksum:i8B209770605AE4BA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000001 Genomic DNA. Translation: BAA30453.1.
PIRiE71006.
RefSeqiWP_010885436.1. NC_000961.1.

Genome annotation databases

EnsemblBacteriaiBAA30453; BAA30453; BAA30453.
GeneIDi1443673.
KEGGipho:PH1347.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000001 Genomic DNA. Translation: BAA30453.1.
PIRiE71006.
RefSeqiWP_010885436.1. NC_000961.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2DPLX-ray1.43A/B1-308[»]
3A4IX-ray1.79A/B1-308[»]
ProteinModelPortaliO59072.
SMRiO59072. Positions 1-308.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi70601.PH1347.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAA30453; BAA30453; BAA30453.
GeneIDi1443673.
KEGGipho:PH1347.

Phylogenomic databases

eggNOGiarCOG00085. Archaea.
COG0519. LUCA.
HOGENOMiHOG000223964.
KOiK01951.
OMAiGGIKSHH.

Enzyme and pathway databases

UniPathwayiUPA00189; UER00296.
BioCyciPHOR70601:GJWR-1354-MONOMER.
BRENDAi6.3.5.2. 5244.

Miscellaneous databases

EvolutionaryTraceiO59072.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_00345. GMP_synthase_B. 1 hit.
InterProiIPR001674. GMP_synth_C.
IPR026598. GMP_synthase_B.
IPR025777. GMPS_ATP_PPase_dom.
IPR022310. NAD/GMP_synthase.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00958. GMP_synt_C. 1 hit.
PF02540. NAD_synthase. 2 hits.
[Graphical view]
TIGRFAMsiTIGR00884. guaA_Cterm. 1 hit.
PROSITEiPS51553. GMPS_ATP_PPASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGUAAB_PYRHO
AccessioniPrimary (citable) accession number: O59072
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: August 1, 1998
Last modified: November 11, 2015
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.