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O59071 (GUAAA_PYRHO) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 76. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
GMP synthase [glutamine-hydrolyzing] subunit A
EC=6.3.5.2
Alternative name(s):
Glutamine amidotransferase
Gene names
Name:guaAA
Ordered Locus Names:PH1346
OrganismPyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3) [Complete proteome] [HAMAP]
Taxonomic identifier70601 [NCBI]
Taxonomic lineageArchaea › Euryarchaeota › Thermococci › Thermococcales › Thermococcaceae › Pyrococcus

Protein attributes

Sequence length189 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the synthesis of GMP from XMP By similarity. HAMAP MF_01510

Catalytic activity

ATP + xanthosine 5'-phosphate + L-glutamine + H2O = AMP + diphosphate + GMP + L-glutamate. HAMAP MF_01510

Pathway

Purine metabolism; GMP biosynthesis; GMP from XMP (L-Gln route): step 1/1. HAMAP MF_01510

Subunit structure

Heterodimer composed of a glutamine amidotransferase subunit (A) and a GMP-binding subunit (B) Potential.

Sequence similarities

Contains 1 glutamine amidotransferase type-1 domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 189189GMP synthase [glutamine-hydrolyzing] subunit A HAMAP MF_01510
PRO_0000140230

Regions

Domain2 – 189188Glutamine amidotransferase type-1

Sites

Active site791Nucleophile By similarity
Active site1661 By similarity
Active site1681 By similarity

Secondary structure

1 ................................... 189
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
O59071 [UniParc].

Last modified August 1, 1998. Version 1.
Checksum: 560F55F7A4BB92A7

FASTA18921,517
        10         20         30         40         50         60 
MMIVIMDNGG QYVHRIWRTL RYLGVETKII PNTTPLEEIK AMNPKGIIFS GGPSLENTGN 

        70         80         90        100        110        120 
CEKVLEHYDE FNVPILGICL GHQLIAKFFG GKVGRGEKAE YSLVEIEIID EDEIFKGLPK 

       130        140        150        160        170        180 
RLKVWESHMD EVKELPPKFK ILARSETCPI EAMKHEELPI YGVQFHPEVA HTEKGEEILR 


NFAKLCGEL

« Hide

References

[1]"Complete sequence and gene organization of the genome of a hyper-thermophilic archaebacterium, Pyrococcus horikoshii OT3."
Kawarabayasi Y., Sawada M., Horikawa H., Haikawa Y., Hino Y., Yamamoto S., Sekine M., Baba S., Kosugi H., Hosoyama A., Nagai Y., Sakai M., Ogura K., Otsuka R., Nakazawa H., Takamiya M., Ohfuku Y., Funahashi T. expand/collapse author list , Tanaka T., Kudoh Y., Yamazaki J., Kushida N., Oguchi A., Aoki K., Yoshizawa T., Nakamura Y., Robb F.T., Horikoshi K., Masuchi Y., Shizuya H., Kikuchi H.
DNA Res. 5:55-76(1998) [PubMed: 9679194] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		BA000001 Genomic DNA. Translation: BAA30452.1.
PIRD71006.
RefSeqNP_143229.1. NC_000961.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1WL8X-ray1.45A1-189[»]
2D7JX-ray1.89A1-189[»]
ProteinModelPortalO59071.
SMRO59071. Positions 2-189.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaEBPYRT00000000399; EBPYRP00000000399; EBPYRG00000000399.
GeneID1443672.
GenomeReviewsGene locus PH1346 in contig BA000001_GR.
KEGGpho:PH1346.
NMPDRfig|70601.1.peg.1325.

Organism-specific databases

CMRSearch...

Phylogenomic databases

GeneTreeEBGT00050000022457.
HOGENOMHBG292341.
OMAGQYVHRI.
PhylomeDBO59071.
ProtClustDBPRK00758.

Enzyme and pathway databases

BioCycPHOR70601:PH1346-MONOMER.

Family and domain databases

HAMAPMF_01510. GMP_synthase_A.
[Tree]
InterProIPR017926. GATASE_1.
IPR004739. GMP_synth_N.
IPR023686. GMP_synthase_A.
[Graphical view]
KOK01951.
PfamPF00117. GATase. 1 hit.
[Graphical view]
TIGRFAMsTIGR00888. GuaA_Nterm. 1 hit.
PROSITEPS51273. GATASE_TYPE_1. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGUAAA_PYRHO
AccessionPrimary (citable) accession number: O59071
Entry history
Integrated into UniProtKB/Swiss-Prot: March 1, 2004
Last sequence update: August 1, 1998
Last modified: January 25, 2012
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

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