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Protein

GMP synthase [glutamine-hydrolyzing] subunit A

Gene

guaAA

Organism
Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the synthesis of GMP from XMP.UniRule annotation

Catalytic activityi

ATP + XMP + L-glutamine + H2O = AMP + diphosphate + GMP + L-glutamate.UniRule annotation

Pathwayi: GMP biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes GMP from XMP (L-Gln route).UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. GMP synthase [glutamine-hydrolyzing] subunit A (guaAA), GMP synthase [glutamine-hydrolyzing] subunit B (guaAB)
This subpathway is part of the pathway GMP biosynthesis, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes GMP from XMP (L-Gln route), the pathway GMP biosynthesis and in Purine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei79NucleophileUniRule annotation1
Active sitei166UniRule annotation1
Active sitei168UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

GMP biosynthesis, Purine biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi6.3.5.2. 5244.
UniPathwayiUPA00189; UER00296.

Protein family/group databases

MEROPSiC26.A31.

Names & Taxonomyi

Protein namesi
Recommended name:
GMP synthase [glutamine-hydrolyzing] subunit AUniRule annotation (EC:6.3.5.2UniRule annotation)
Alternative name(s):
Glutamine amidotransferaseUniRule annotation
Gene namesi
Name:guaAAUniRule annotation
Ordered Locus Names:PH1346
OrganismiPyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Taxonomic identifieri70601 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
Proteomesi
  • UP000000752 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001402301 – 189GMP synthase [glutamine-hydrolyzing] subunit AAdd BLAST189

Interactioni

Subunit structurei

Heterodimer composed of a glutamine amidotransferase subunit (A) and a GMP-binding subunit (B).UniRule annotation

Protein-protein interaction databases

STRINGi70601.PH1346.

Structurei

Secondary structure

1189
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi2 – 7Combined sources6
Helixi13 – 22Combined sources10
Beta strandi26 – 31Combined sources6
Helixi36 – 41Combined sources6
Beta strandi45 – 49Combined sources5
Helixi61 – 66Combined sources6
Helixi68 – 70Combined sources3
Beta strandi75 – 78Combined sources4
Helixi80 – 89Combined sources10
Beta strandi92 – 95Combined sources4
Beta strandi102 – 109Combined sources8
Turni112 – 117Combined sources6
Beta strandi120 – 126Combined sources7
Beta strandi129 – 134Combined sources6
Beta strandi139 – 147Combined sources9
Beta strandi151 – 158Combined sources8
Beta strandi160 – 165Combined sources6
Helixi175 – 186Combined sources12

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1WL8X-ray1.45A1-189[»]
2D7JX-ray1.89A1-189[»]
ProteinModelPortaliO59071.
SMRiO59071.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO59071.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 189Glutamine amidotransferase type-1UniRule annotationAdd BLAST188

Sequence similaritiesi

Contains 1 glutamine amidotransferase type-1 domain.UniRule annotation

Keywords - Domaini

Glutamine amidotransferase

Phylogenomic databases

eggNOGiarCOG00087. Archaea.
COG0518. LUCA.
HOGENOMiHOG000025031.
KOiK01951.
OMAiKVWESHM.

Family and domain databases

CDDicd01742. GATase1_GMP_Synthase. 1 hit.
Gene3Di3.40.50.880. 1 hit.
HAMAPiMF_01510. GMP_synthase_A. 1 hit.
InterProiIPR029062. Class_I_gatase-like.
IPR017926. GATASE.
IPR004739. GMP_synth_GATase.
IPR023686. GMP_synthase_A.
[Graphical view]
PfamiPF00117. GATase. 1 hit.
[Graphical view]
SUPFAMiSSF52317. SSF52317. 1 hit.
TIGRFAMsiTIGR00888. guaA_Nterm. 1 hit.
PROSITEiPS51273. GATASE_TYPE_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O59071-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMIVIMDNGG QYVHRIWRTL RYLGVETKII PNTTPLEEIK AMNPKGIIFS
60 70 80 90 100
GGPSLENTGN CEKVLEHYDE FNVPILGICL GHQLIAKFFG GKVGRGEKAE
110 120 130 140 150
YSLVEIEIID EDEIFKGLPK RLKVWESHMD EVKELPPKFK ILARSETCPI
160 170 180
EAMKHEELPI YGVQFHPEVA HTEKGEEILR NFAKLCGEL
Length:189
Mass (Da):21,517
Last modified:August 1, 1998 - v1
Checksum:i560F55F7A4BB92A7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000001 Genomic DNA. Translation: BAA30452.1.
PIRiD71006.

Genome annotation databases

EnsemblBacteriaiBAA30452; BAA30452; BAA30452.
KEGGipho:PH1346.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000001 Genomic DNA. Translation: BAA30452.1.
PIRiD71006.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1WL8X-ray1.45A1-189[»]
2D7JX-ray1.89A1-189[»]
ProteinModelPortaliO59071.
SMRiO59071.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi70601.PH1346.

Protein family/group databases

MEROPSiC26.A31.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAA30452; BAA30452; BAA30452.
KEGGipho:PH1346.

Phylogenomic databases

eggNOGiarCOG00087. Archaea.
COG0518. LUCA.
HOGENOMiHOG000025031.
KOiK01951.
OMAiKVWESHM.

Enzyme and pathway databases

UniPathwayiUPA00189; UER00296.
BRENDAi6.3.5.2. 5244.

Miscellaneous databases

EvolutionaryTraceiO59071.

Family and domain databases

CDDicd01742. GATase1_GMP_Synthase. 1 hit.
Gene3Di3.40.50.880. 1 hit.
HAMAPiMF_01510. GMP_synthase_A. 1 hit.
InterProiIPR029062. Class_I_gatase-like.
IPR017926. GATASE.
IPR004739. GMP_synth_GATase.
IPR023686. GMP_synthase_A.
[Graphical view]
PfamiPF00117. GATase. 1 hit.
[Graphical view]
SUPFAMiSSF52317. SSF52317. 1 hit.
TIGRFAMsiTIGR00888. guaA_Nterm. 1 hit.
PROSITEiPS51273. GATASE_TYPE_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGUAAA_PYRHO
AccessioniPrimary (citable) accession number: O59071
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 2004
Last sequence update: August 1, 1998
Last modified: November 2, 2016
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.