Skip Header

Contribute Send feedback
Read comments (?) or add your own

O59028 (MDH_PYRHO) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 64. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Malate dehydrogenase
EC=1.1.1.37
Gene names
Name:mdh
Ordered Locus Names:PH1277
OrganismPyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3) [Complete proteome] [HAMAP]
Taxonomic identifier70601 [NCBI]
Taxonomic lineageArchaea › Euryarchaeota › Thermococci › Thermococcales › Thermococcaceae › Pyrococcus

Protein attributes

Sequence length360 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

(S)-malate + NAD+ = oxaloacetate + NADH.

Subunit structure

Homodimer By similarity.

Subcellular location

Cytoplasm Probable.

Sequence similarities

Belongs to the LDH2/MDH2 oxidoreductase family.

Ontologies

Keywords
   Biological processTricarboxylic acid cycle
   Cellular componentCytoplasm
   LigandNAD
   Molecular functionOxidoreductase
   Technical term3D-structure
Complete proteome
Gene Ontology (GO)
   Biological processtricarboxylic acid cycle

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionL-malate dehydrogenase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 360360Malate dehydrogenase
PRO_0000083829

Secondary structure

1 ......................................................... 360
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
O59028 [UniParc].

Last modified August 1, 1998. Version 1.
Checksum: 6E9D8B16ECDE6E6F

FASTA36039,751
        10         20         30         40         50         60 
MFEKGYVDEN YIRVPKDRLF SFIVRVLTKL GVPEEDAKIV ADNLVMADLR GVESHGVQRL 

        70         80         90        100        110        120 
KRYVDGIISG GVNLHPKIRV IREGPSYALI DGDEGLGQVV GYRSMKLAIK KAKDTGIGIV 

       130        140        150        160        170        180 
IARNSNHYGI AGYYALMAAE EGMIGISMTN SRPLVAPTGG IERILGTNPI ALAAPTKDKP 

       190        200        210        220        230        240 
FLLDMATSVV PIGKLEVYRR KGKDIPEGWA INREGNITTK VEEVFNGGAL LPLGGFGELL 

       250        260        270        280        290        300 
GGHKGYGLSL MVDILSGILS GGTWSKYVKN TSEKGSNVCH FFMVIDIEHF IPLEEFKEKI 

       310        320        330        340        350        360 
SQMIEEIKSS RKHPEFERIW IHGEKGFLTM ETRLKLGIPI YRKVLEELNE IAKRVGVEGL

« Hide

References

[1]"Complete sequence and gene organization of the genome of a hyper-thermophilic archaebacterium, Pyrococcus horikoshii OT3."
Kawarabayasi Y., Sawada M., Horikawa H., Haikawa Y., Hino Y., Yamamoto S., Sekine M., Baba S., Kosugi H., Hosoyama A., Nagai Y., Sakai M., Ogura K., Otsuka R., Nakazawa H., Takamiya M., Ohfuku Y., Funahashi T. expand/collapse author list , Tanaka T., Kudoh Y., Yamazaki J., Kushida N., Oguchi A., Aoki K., Yoshizawa T., Nakamura Y., Robb F.T., Horikoshi K., Masuchi Y., Shizuya H., Kikuchi H.
DNA Res. 5:55-76(1998) [PubMed: 9679194] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		BA000001 Genomic DNA. Translation: BAA30380.1.
PIRB71073.
RefSeqNP_143166.1. NC_000961.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1V9NX-ray2.10A1-360[»]
ProteinModelPortalO59028.
SMRO59028. Positions 1-360.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaEBPYRT00000000749; EBPYRP00000000749; EBPYRG00000000749.
GeneID1443600.
GenomeReviewsGene locus PH1277 in contig BA000001_GR.
KEGGpho:PH1277.
NMPDRfig|70601.1.peg.1253.

Organism-specific databases

CMRSearch...

Phylogenomic databases

GeneTreeEBGT00050000022443.
HOGENOMHBG553037.
OMAMYINDLA.
PhylomeDBO59028.
ProtClustDBCLSK865277.

Enzyme and pathway databases

BioCycPHOR70601:PH1277-MONOMER.

Family and domain databases

InterProIPR003767. Malate/L-lactate_DH.
[Graphical view]
KOK05884.
PANTHERPTHR11091. MDH_LDH. 1 hit.
PfamPF02615. Ldh_2. 1 hit.
[Graphical view]
SUPFAMSSF89733. MDH_LDH. 1 hit.
ProtoNetSearch...

Entry information

Entry nameMDH_PYRHO
AccessionPrimary (citable) accession number: O59028
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: August 1, 1998
Last modified: December 14, 2011
This is version 64 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents