Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Phosphoglycerate kinase

Gene

pgk

Organism
Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + 3-phospho-D-glycerate = ADP + 3-phospho-D-glyceroyl phosphate.

Pathwayi: glycolysis

This protein is involved in step 2 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Glyceraldehyde-3-phosphate dehydrogenase (gap)
  2. Phosphoglycerate kinase (pgk)
  3. 2,3-bisphosphoglycerate-independent phosphoglycerate mutase (apgM)
  4. Enolase (eno)
  5. Pyruvate kinase (PH0570)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei34Substrate1 Publication1
Binding sitei114SubstrateBy similarity1
Binding sitei154SubstrateBy similarity1
Binding sitei332ATPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi358 – 361ATPBy similarity4

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00109; UER00185.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoglycerate kinase (EC:2.7.2.3)
Gene namesi
Name:pgk
Ordered Locus Names:PH1218
ORF Names:PHBK036
OrganismiPyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Taxonomic identifieri70601 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
Proteomesi
  • UP000000752 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001460671 – 410Phosphoglycerate kinaseAdd BLAST410

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

IntActiO58965. 1 interactor.
MINTiMINT-1502980.
STRINGi70601.PH1218.

Structurei

Secondary structure

1410
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi4 – 6Combined sources3
Beta strandi13 – 17Combined sources5
Helixi33 – 37Combined sources5
Helixi39 – 47Combined sources9
Beta strandi51 – 55Combined sources5
Helixi70 – 80Combined sources11
Beta strandi84 – 86Combined sources3
Beta strandi90 – 92Combined sources3
Helixi93 – 100Combined sources8
Beta strandi107 – 109Combined sources3
Helixi113 – 115Combined sources3
Turni117 – 120Combined sources4
Helixi125 – 128Combined sources4
Helixi132 – 138Combined sources7
Beta strandi142 – 146Combined sources5
Helixi149 – 151Combined sources3
Helixi157 – 160Combined sources4
Turni161 – 165Combined sources5
Beta strandi168 – 170Combined sources3
Helixi172 – 185Combined sources14
Beta strandi192 – 196Combined sources5
Helixi201 – 213Combined sources13
Beta strandi218 – 222Combined sources5
Helixi224 – 234Combined sources11
Helixi240 – 247Combined sources8
Turni248 – 250Combined sources3
Helixi251 – 254Combined sources4
Helixi255 – 265Combined sources11
Helixi266 – 268Combined sources3
Beta strandi273 – 279Combined sources7
Beta strandi282 – 290Combined sources9
Helixi291 – 297Combined sources7
Beta strandi301 – 304Combined sources4
Helixi306 – 317Combined sources12
Beta strandi320 – 326Combined sources7
Helixi334 – 336Combined sources3
Helixi338 – 349Combined sources12
Beta strandi350 – 357Combined sources8
Helixi359 – 367Combined sources9
Beta strandi374 – 376Combined sources3
Helixi381 – 387Combined sources7
Helixi393 – 404Combined sources12

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2CUNX-ray2.10A/B1-410[»]
ProteinModelPortaliO58965.
SMRiO58965.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO58965.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni19 – 21Substrate bindingBy similarity3
Regioni57 – 60Substrate bindingBy similarity4

Sequence similaritiesi

Belongs to the phosphoglycerate kinase family.Curated

Phylogenomic databases

eggNOGiarCOG00496. Archaea.
COG0126. LUCA.
HOGENOMiHOG000227108.
KOiK00927.
OMAiITMAKKS.

Family and domain databases

Gene3Di3.40.50.1260. 1 hit.
3.40.50.1270. 1 hit.
HAMAPiMF_00145. Phosphoglyc_kinase. 1 hit.
InterProiIPR001576. Phosphoglycerate_kinase.
IPR015901. Phosphoglycerate_kinase_C.
IPR015911. Phosphoglycerate_kinase_CS.
IPR015824. Phosphoglycerate_kinase_N.
[Graphical view]
PANTHERiPTHR11406. PTHR11406. 1 hit.
PfamiPF00162. PGK. 1 hit.
[Graphical view]
PIRSFiPIRSF000724. Pgk. 1 hit.
PRINTSiPR00477. PHGLYCKINASE.
SUPFAMiSSF53748. SSF53748. 1 hit.
PROSITEiPS00111. PGLYCERATE_KINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O58965-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFRLEDFNFH NKTVFLRVDL NSPMKDGKII SDARFKAVLP TIRYLIESGA
60 70 80 90 100
KVVIGTHQGK PYSEDYTTTE EHARVLSELL DQHVEYIEDI FGRYAREKIK
110 120 130 140 150
ELKSGEVAIL ENLRFSAEEV KNKPIEECEK TFLVKKLSKV IDYVVNDAFA
160 170 180 190 200
TAHRSQPSLV GFARIKPMIM GFLMEKEIEA LMRAYYSKDS PKIYVLGGAK
210 220 230 240 250
VEDSLKVVEN VLRRERADLV LTGGLVANVF TLAKGFDLGR KNVEFMKKKG
260 270 280 290 300
LLDYVKHAEE ILDEFYPYIR TPVDFAVDYK GERVEIDLLS ENRGLLHQYQ
310 320 330 340 350
IMDIGKRTAE KYREILMKAR IIVANGPMGV FEREEFAIGT VEVFKAIADS
360 370 380 390 400
PAFSVLGGGH SIASIQKYGI TGITHISTGG GAMLSFFAGE ELPVLRALQI
410
SYEKFKEVVK
Length:410
Mass (Da):46,403
Last modified:August 1, 1998 - v1
Checksum:i6912EDE7BD26486E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000001 Genomic DNA. Translation: BAA30318.1.
PIRiD71065.
RefSeqiWP_010885305.1. NC_000961.1.

Genome annotation databases

EnsemblBacteriaiBAA30318; BAA30318; BAA30318.
GeneIDi1443540.
KEGGipho:PH1218.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000001 Genomic DNA. Translation: BAA30318.1.
PIRiD71065.
RefSeqiWP_010885305.1. NC_000961.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2CUNX-ray2.10A/B1-410[»]
ProteinModelPortaliO58965.
SMRiO58965.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiO58965. 1 interactor.
MINTiMINT-1502980.
STRINGi70601.PH1218.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAA30318; BAA30318; BAA30318.
GeneIDi1443540.
KEGGipho:PH1218.

Phylogenomic databases

eggNOGiarCOG00496. Archaea.
COG0126. LUCA.
HOGENOMiHOG000227108.
KOiK00927.
OMAiITMAKKS.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00185.

Miscellaneous databases

EvolutionaryTraceiO58965.

Family and domain databases

Gene3Di3.40.50.1260. 1 hit.
3.40.50.1270. 1 hit.
HAMAPiMF_00145. Phosphoglyc_kinase. 1 hit.
InterProiIPR001576. Phosphoglycerate_kinase.
IPR015901. Phosphoglycerate_kinase_C.
IPR015911. Phosphoglycerate_kinase_CS.
IPR015824. Phosphoglycerate_kinase_N.
[Graphical view]
PANTHERiPTHR11406. PTHR11406. 1 hit.
PfamiPF00162. PGK. 1 hit.
[Graphical view]
PIRSFiPIRSF000724. Pgk. 1 hit.
PRINTSiPR00477. PHGLYCKINASE.
SUPFAMiSSF53748. SSF53748. 1 hit.
PROSITEiPS00111. PGLYCERATE_KINASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPGK_PYRHO
AccessioniPrimary (citable) accession number: O58965
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: August 1, 1998
Last modified: November 2, 2016
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.