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O58954

- AGOG_PYRHO

UniProt

O58954 - AGOG_PYRHO

Protein

N-glycosylase/DNA lyase

Gene

PH1229

Organism
Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 71 (01 Oct 2014)
      Sequence version 1 (01 Aug 1998)
      Previous versions | rss
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    Functioni

    DNA repair enzyme that is part of the base excision repair (BER) pathway; protects from oxidative damage by removing the major product of DNA oxidation, 8-oxoguanine (GO), from single- and double-stranded DNA substrates.UniRule annotation

    Catalytic activityi

    The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei24 – 2418-oxoguanineUniRule annotation
    Binding sitei51 – 5118-oxoguanine; via carbonyl oxygenUniRule annotation
    Binding sitei62 – 6218-oxoguanineUniRule annotation
    Active sitei142 – 1421Schiff-base intermediate with DNAUniRule annotation
    Binding sitei146 – 14618-oxoguanineUniRule annotation
    Binding sitei172 – 17218-oxoguanine; via carbonyl oxygenUniRule annotation
    Active sitei174 – 1741UniRule annotation
    Binding sitei208 – 20818-oxoguanineUniRule annotation
    Binding sitei212 – 21218-oxoguanineUniRule annotation

    GO - Molecular functioni

    1. DNA-(apurinic or apyrimidinic site) lyase activity Source: UniProtKB-HAMAP
    2. oxidized base lesion DNA N-glycosylase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. base-excision repair Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Hydrolase, Lyase

    Keywords - Biological processi

    DNA damage, DNA excision, DNA repair

    Enzyme and pathway databases

    BioCyciPHOR70601:GJWR-1224-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    N-glycosylase/DNA lyaseUniRule annotation
    Alternative name(s):
    8-oxoguanine DNA glycosylaseUniRule annotation (EC:3.2.2.-UniRule annotation)
    AGOGUniRule annotation
    DNA-(apurinic or apyrimidinic site) lyaseUniRule annotation (EC:4.2.99.18UniRule annotation)
    Short name:
    AP lyaseUniRule annotation
    Gene namesi
    Ordered Locus Names:PH1229
    OrganismiPyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
    Taxonomic identifieri70601 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
    ProteomesiUP000000752: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 239239N-glycosylase/DNA lyasePRO_0000185115Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi70601.PH1229.

    Structurei

    3D structure databases

    ProteinModelPortaliO58954.
    SMRiO58954. Positions 2-238.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni118 – 18265Helix-hairpin-helixAdd
    BLAST

    Domaini

    Contains two alpha-helical subdomains, with the 8-oxoguanine binding site located in a cleft at their interface. Contains a helix-hairpin-helix (HhH) structural motif and a Gly/Pro-rich sequence followed by a conserved Asp (HhH-GPD motif).

    Sequence similaritiesi

    Belongs to the archaeal N-glycosylase/DNA lyase (AGOG) family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG4047.
    HOGENOMiHOG000254352.
    KOiK01741.
    OMAiVKMFGYA.

    Family and domain databases

    Gene3Di1.10.1670.10. 1 hit.
    1.10.340.30. 1 hit.
    HAMAPiMF_01168. AGOG.
    InterProiIPR011257. DNA_glycosylase.
    IPR023170. HTH_base_excis_C.
    IPR015254. N-Glyclase/DNA_lyase-like_arc.
    IPR016544. N-Glyclase/DNA_lyase_arc.
    [Graphical view]
    PfamiPF09171. DUF1886. 1 hit.
    [Graphical view]
    PIRSFiPIRSF008955. AGOG. 1 hit.
    SUPFAMiSSF48150. SSF48150. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    O58954-1 [UniParc]FASTAAdd to Basket

    « Hide

    MIAELIREIG IEGARFIEEN IDEQFKALSY LSEGMDRVNF VRLVIANALV    50
    SYQLTGKGEM WWWEFAKYFK GKEVKTIYSA YKEFLPNSKF NRRLIQQKLL 100
    RIKKIEPFLS TLTEESIERY YEDMTLLWKA IAKVLKVDRE SKTVVFSVKM 150
    FGYAARIVLS KFNPYPMEIP IPEDVRIIKL TRKLTNERPR DFWMKIAKES 200
    NVPPLHIDSI LWPLLGGARV EEAPPELKEK LEKLIRVIR 239
    Length:239
    Mass (Da):28,191
    Last modified:August 1, 1998 - v1
    Checksum:i5EC26389EBB5B99E
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000001 Genomic DNA. Translation: BAA30329.1.
    PIRiG71066.
    RefSeqiNP_143123.1. NC_000961.1.
    WP_010885316.1. NC_000961.1.

    Genome annotation databases

    EnsemblBacteriaiBAA30329; BAA30329; BAA30329.
    GeneIDi1443551.
    KEGGipho:PH1229.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000001 Genomic DNA. Translation: BAA30329.1 .
    PIRi G71066.
    RefSeqi NP_143123.1. NC_000961.1.
    WP_010885316.1. NC_000961.1.

    3D structure databases

    ProteinModelPortali O58954.
    SMRi O58954. Positions 2-238.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 70601.PH1229.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAA30329 ; BAA30329 ; BAA30329 .
    GeneIDi 1443551.
    KEGGi pho:PH1229.

    Phylogenomic databases

    eggNOGi COG4047.
    HOGENOMi HOG000254352.
    KOi K01741.
    OMAi VKMFGYA.

    Enzyme and pathway databases

    BioCyci PHOR70601:GJWR-1224-MONOMER.

    Family and domain databases

    Gene3Di 1.10.1670.10. 1 hit.
    1.10.340.30. 1 hit.
    HAMAPi MF_01168. AGOG.
    InterProi IPR011257. DNA_glycosylase.
    IPR023170. HTH_base_excis_C.
    IPR015254. N-Glyclase/DNA_lyase-like_arc.
    IPR016544. N-Glyclase/DNA_lyase_arc.
    [Graphical view ]
    Pfami PF09171. DUF1886. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF008955. AGOG. 1 hit.
    SUPFAMi SSF48150. SSF48150. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3.

    Entry informationi

    Entry nameiAGOG_PYRHO
    AccessioniPrimary (citable) accession number: O58954
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 10, 2006
    Last sequence update: August 1, 1998
    Last modified: October 1, 2014
    This is version 71 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3