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O58954

- AGOG_PYRHO

UniProt

O58954 - AGOG_PYRHO

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Protein
N-glycosylase/DNA lyase
Gene
PH1229
Organism
Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

DNA repair enzyme that is part of the base excision repair (BER) pathway; protects from oxidative damage by removing the major product of DNA oxidation, 8-oxoguanine (GO), from single- and double-stranded DNA substrates By similarity.UniRule annotation

Catalytic activityi

The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei24 – 2418-oxoguanine By similarity
Binding sitei51 – 5118-oxoguanine; via carbonyl oxygen By similarity
Binding sitei62 – 6218-oxoguanine By similarity
Active sitei142 – 1421Schiff-base intermediate with DNA By similarity
Binding sitei146 – 14618-oxoguanine By similarity
Binding sitei172 – 17218-oxoguanine; via carbonyl oxygen By similarity
Active sitei174 – 1741 Reviewed prediction
Binding sitei208 – 20818-oxoguanine By similarity
Binding sitei212 – 21218-oxoguanine By similarity

GO - Molecular functioni

  1. DNA-(apurinic or apyrimidinic site) lyase activity Source: UniProtKB-HAMAP
  2. oxidized base lesion DNA N-glycosylase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. base-excision repair Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Lyase

Keywords - Biological processi

DNA damage, DNA excision, DNA repair

Enzyme and pathway databases

BioCyciPHOR70601:GJWR-1224-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
N-glycosylase/DNA lyase
Alternative name(s):
8-oxoguanine DNA glycosylase (EC:3.2.2.-)
AGOG
DNA-(apurinic or apyrimidinic site) lyase (EC:4.2.99.18)
Short name:
AP lyase
Gene namesi
Ordered Locus Names:PH1229
OrganismiPyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Taxonomic identifieri70601 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
ProteomesiUP000000752: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 239239N-glycosylase/DNA lyaseUniRule annotation
PRO_0000185115Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi70601.PH1229.

Structurei

3D structure databases

ProteinModelPortaliO58954.
SMRiO58954. Positions 2-238.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni118 – 18265Helix-hairpin-helixUniRule annotation
Add
BLAST

Domaini

Contains two alpha-helical subdomains, with the 8-oxoguanine binding site located in a cleft at their interface. Contains a helix-hairpin-helix (HhH) structural motif and a Gly/Pro-rich sequence followed by a conserved Asp (HhH-GPD motif).UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG4047.
HOGENOMiHOG000254352.
KOiK01741.
OMAiVKMFGYA.

Family and domain databases

Gene3Di1.10.1670.10. 1 hit.
1.10.340.30. 1 hit.
HAMAPiMF_01168. AGOG.
InterProiIPR011257. DNA_glycosylase.
IPR023170. HTH_base_excis_C.
IPR015254. N-Glyclase/DNA_lyase-like_arc.
IPR016544. N-Glyclase/DNA_lyase_arc.
[Graphical view]
PfamiPF09171. DUF1886. 1 hit.
[Graphical view]
PIRSFiPIRSF008955. AGOG. 1 hit.
SUPFAMiSSF48150. SSF48150. 1 hit.

Sequencei

Sequence statusi: Complete.

O58954-1 [UniParc]FASTAAdd to Basket

« Hide

MIAELIREIG IEGARFIEEN IDEQFKALSY LSEGMDRVNF VRLVIANALV    50
SYQLTGKGEM WWWEFAKYFK GKEVKTIYSA YKEFLPNSKF NRRLIQQKLL 100
RIKKIEPFLS TLTEESIERY YEDMTLLWKA IAKVLKVDRE SKTVVFSVKM 150
FGYAARIVLS KFNPYPMEIP IPEDVRIIKL TRKLTNERPR DFWMKIAKES 200
NVPPLHIDSI LWPLLGGARV EEAPPELKEK LEKLIRVIR 239
Length:239
Mass (Da):28,191
Last modified:August 1, 1998 - v1
Checksum:i5EC26389EBB5B99E
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000001 Genomic DNA. Translation: BAA30329.1.
PIRiG71066.
RefSeqiNP_143123.1. NC_000961.1.
WP_010885316.1. NC_000961.1.

Genome annotation databases

EnsemblBacteriaiBAA30329; BAA30329; BAA30329.
GeneIDi1443551.
KEGGipho:PH1229.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000001 Genomic DNA. Translation: BAA30329.1 .
PIRi G71066.
RefSeqi NP_143123.1. NC_000961.1.
WP_010885316.1. NC_000961.1.

3D structure databases

ProteinModelPortali O58954.
SMRi O58954. Positions 2-238.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 70601.PH1229.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAA30329 ; BAA30329 ; BAA30329 .
GeneIDi 1443551.
KEGGi pho:PH1229.

Phylogenomic databases

eggNOGi COG4047.
HOGENOMi HOG000254352.
KOi K01741.
OMAi VKMFGYA.

Enzyme and pathway databases

BioCyci PHOR70601:GJWR-1224-MONOMER.

Family and domain databases

Gene3Di 1.10.1670.10. 1 hit.
1.10.340.30. 1 hit.
HAMAPi MF_01168. AGOG.
InterProi IPR011257. DNA_glycosylase.
IPR023170. HTH_base_excis_C.
IPR015254. N-Glyclase/DNA_lyase-like_arc.
IPR016544. N-Glyclase/DNA_lyase_arc.
[Graphical view ]
Pfami PF09171. DUF1886. 1 hit.
[Graphical view ]
PIRSFi PIRSF008955. AGOG. 1 hit.
SUPFAMi SSF48150. SSF48150. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3.

Entry informationi

Entry nameiAGOG_PYRHO
AccessioniPrimary (citable) accession number: O58954
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 10, 2006
Last sequence update: August 1, 1998
Last modified: September 3, 2014
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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