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Protein

N-glycosylase/DNA lyase

Gene

PH1229

Organism
Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

DNA repair enzyme that is part of the base excision repair (BER) pathway; protects from oxidative damage by removing the major product of DNA oxidation, 8-oxoguanine (GO), from single- and double-stranded DNA substrates.UniRule annotation

Catalytic activityi

The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei248-oxoguanineUniRule annotation1
Binding sitei518-oxoguanine; via carbonyl oxygenUniRule annotation1
Binding sitei628-oxoguanineUniRule annotation1
Active sitei142Schiff-base intermediate with DNAUniRule annotation1
Binding sitei1468-oxoguanineUniRule annotation1
Binding sitei1728-oxoguanine; via carbonyl oxygenUniRule annotation1
Active sitei174UniRule annotation1
Binding sitei2088-oxoguanineUniRule annotation1
Binding sitei2128-oxoguanineUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Lyase
Biological processDNA damage, DNA excision, DNA repair

Enzyme and pathway databases

BioCyciPHOR70601:G1G39-1189-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
N-glycosylase/DNA lyaseUniRule annotation
Alternative name(s):
8-oxoguanine DNA glycosylaseUniRule annotation (EC:3.2.2.-UniRule annotation)
AGOGUniRule annotation
DNA-(apurinic or apyrimidinic site) lyaseUniRule annotation (EC:4.2.99.18UniRule annotation)
Short name:
AP lyaseUniRule annotation
Gene namesi
Ordered Locus Names:PH1229
OrganismiPyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Taxonomic identifieri70601 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
Proteomesi
  • UP000000752 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001851151 – 239N-glycosylase/DNA lyaseAdd BLAST239

Interactioni

Protein-protein interaction databases

STRINGi70601.PH1229

Structurei

3D structure databases

ProteinModelPortaliO58954
SMRiO58954
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni118 – 182Helix-hairpin-helixAdd BLAST65

Domaini

Contains two alpha-helical subdomains, with the 8-oxoguanine binding site located in a cleft at their interface. Contains a helix-hairpin-helix (HhH) structural motif and a Gly/Pro-rich sequence followed by a conserved Asp (HhH-GPD motif).

Sequence similaritiesi

Belongs to the archaeal N-glycosylase/DNA lyase (AGOG) family.UniRule annotation

Phylogenomic databases

eggNOGiarCOG04144 Archaea
COG4047 LUCA
HOGENOMiHOG000254352
KOiK01741
OMAiVKMFGYA
OrthoDBiPOG093Z09FD

Family and domain databases

Gene3Di1.10.1670.10, 2 hits
HAMAPiMF_01168 AGOG, 1 hit
InterProiView protein in InterPro
IPR016544 AGOG
IPR015254 AGOG-like
IPR011257 DNA_glycosylase
IPR023170 HTH_base_excis_C
PfamiView protein in Pfam
PF09171 AGOG, 1 hit
PIRSFiPIRSF008955 AGOG, 1 hit
SUPFAMiSSF48150 SSF48150, 1 hit

Sequencei

Sequence statusi: Complete.

O58954-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIAELIREIG IEGARFIEEN IDEQFKALSY LSEGMDRVNF VRLVIANALV
60 70 80 90 100
SYQLTGKGEM WWWEFAKYFK GKEVKTIYSA YKEFLPNSKF NRRLIQQKLL
110 120 130 140 150
RIKKIEPFLS TLTEESIERY YEDMTLLWKA IAKVLKVDRE SKTVVFSVKM
160 170 180 190 200
FGYAARIVLS KFNPYPMEIP IPEDVRIIKL TRKLTNERPR DFWMKIAKES
210 220 230
NVPPLHIDSI LWPLLGGARV EEAPPELKEK LEKLIRVIR
Length:239
Mass (Da):28,191
Last modified:August 1, 1998 - v1
Checksum:i5EC26389EBB5B99E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000001 Genomic DNA Translation: BAA30329.1
PIRiG71066
RefSeqiWP_010885316.1, NC_000961.1

Genome annotation databases

EnsemblBacteriaiBAA30329; BAA30329; BAA30329
GeneIDi1443551
KEGGipho:PH1229

Similar proteinsi

Entry informationi

Entry nameiAGOG_PYRHO
AccessioniPrimary (citable) accession number: O58954
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 10, 2006
Last sequence update: August 1, 1998
Last modified: April 25, 2018
This is version 88 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health