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Protein

Ribonuclease Z

Gene

rnz

Organism
Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Zinc phosphodiesterase, which displays some tRNA 3'-processing endonuclease activity. Probably involved in tRNA maturation, by removing a 3'-trailer from precursor tRNA.UniRule annotation

Catalytic activityi

Endonucleolytic cleavage of RNA, removing extra 3' nucleotides from tRNA precursor, generating 3' termini of tRNAs. A 3'-hydroxy group is left at the tRNA terminus and a 5'-phosphoryl group is left at the trailer molecule.UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 2 Zn2+ ions.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi61 – 611Zinc 1; catalyticUniRule annotation
Metal bindingi63 – 631Zinc 1; catalyticUniRule annotation
Active sitei65 – 651Proton acceptorUniRule annotation
Metal bindingi65 – 651Zinc 2; catalyticUniRule annotation
Metal bindingi66 – 661Zinc 2; catalyticUniRule annotation
Metal bindingi138 – 1381Zinc 1; catalyticUniRule annotation
Metal bindingi208 – 2081Zinc 1; catalyticUniRule annotation
Metal bindingi208 – 2081Zinc 2; catalyticUniRule annotation
Metal bindingi264 – 2641Zinc 2; catalyticUniRule annotation

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciPHOR70601:GJWR-1142-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonuclease ZUniRule annotation (EC:3.1.26.11UniRule annotation)
Short name:
RNase ZUniRule annotation
Alternative name(s):
tRNA 3 endonucleaseUniRule annotation
tRNase ZUniRule annotation
Gene namesi
Name:rnzUniRule annotation
Ordered Locus Names:PH1151
OrganismiPyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Taxonomic identifieri70601 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
Proteomesi
  • UP000000752 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 307307Ribonuclease ZPRO_0000155934Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi70601.PH1151.

Structurei

3D structure databases

ProteinModelPortaliO58883.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the RNase Z family.UniRule annotation

Phylogenomic databases

eggNOGiarCOG00501. Archaea.
COG1234. LUCA.
HOGENOMiHOG000272419.
KOiK00784.
OMAiSYAFCSD.

Family and domain databases

Gene3Di3.60.15.10. 1 hit.
HAMAPiMF_01818. RNase_Z_BN.
InterProiIPR001279. Metallo-B-lactamas.
IPR013471. RNase_Z/BN.
[Graphical view]
PfamiPF00753. Lactamase_B. 1 hit.
PF12706. Lactamase_B_2. 1 hit.
[Graphical view]
SMARTiSM00849. Lactamase_B. 1 hit.
[Graphical view]
SUPFAMiSSF56281. SSF56281. 1 hit.
TIGRFAMsiTIGR02651. RNase_Z. 1 hit.

Sequencei

Sequence statusi: Complete.

O58883-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIEVIFLGTG GIKPTPERNV PSIAIKIGGE IILFDVGEGT LRQMEIARIS
60 70 80 90 100
PMKIKRVFIT HFHGDHYLGL PALIQTMSLW KRKDPLHIYG PEGSSTFLQN
110 120 130 140 150
LLNSGYFAPS FDILVHEISG KSRLKFKEYE VWAFEVSHGV PALGYVFKEK
160 170 180 190 200
DKRGNFNLKK IKELGLEPGP WMKELERQKI IEINGKIVRL LEVTGPKKRG
210 220 230 240 250
AKVVYSGDTE PCDEVIEFSR RGTLLIHEAT YVNEEDRKDS YHTTIEEACE
260 270 280 290 300
IWKKSKARKL VLFHRSPRYS FKEYKEKALS ICPQAIIPRD FDRIAIEGAG

DVLFKIR
Length:307
Mass (Da):35,131
Last modified:August 1, 1998 - v1
Checksum:i602F289A2739BD04
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000001 Genomic DNA. Translation: BAA30251.1.
PIRiA71057.
RefSeqiWP_010885235.1. NC_000961.1.

Genome annotation databases

EnsemblBacteriaiBAA30251; BAA30251; BAA30251.
GeneIDi1443470.
KEGGipho:PH1151.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000001 Genomic DNA. Translation: BAA30251.1.
PIRiA71057.
RefSeqiWP_010885235.1. NC_000961.1.

3D structure databases

ProteinModelPortaliO58883.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi70601.PH1151.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAA30251; BAA30251; BAA30251.
GeneIDi1443470.
KEGGipho:PH1151.

Phylogenomic databases

eggNOGiarCOG00501. Archaea.
COG1234. LUCA.
HOGENOMiHOG000272419.
KOiK00784.
OMAiSYAFCSD.

Enzyme and pathway databases

BioCyciPHOR70601:GJWR-1142-MONOMER.

Family and domain databases

Gene3Di3.60.15.10. 1 hit.
HAMAPiMF_01818. RNase_Z_BN.
InterProiIPR001279. Metallo-B-lactamas.
IPR013471. RNase_Z/BN.
[Graphical view]
PfamiPF00753. Lactamase_B. 1 hit.
PF12706. Lactamase_B_2. 1 hit.
[Graphical view]
SMARTiSM00849. Lactamase_B. 1 hit.
[Graphical view]
SUPFAMiSSF56281. SSF56281. 1 hit.
TIGRFAMsiTIGR02651. RNase_Z. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3.

Entry informationi

Entry nameiRNZ_PYRHO
AccessioniPrimary (citable) accession number: O58883
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 16, 2004
Last sequence update: August 1, 1998
Last modified: May 11, 2016
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.