O58883 (RNZ_PYRHO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribonuclease Z
Short name=RNase Z
EC=3.1.26.11

Alternative name(s):
tRNA 3 endonuclease
tRNase Z

Gene names

Name:	rnz
Ordered Locus Names:	PH1151

Organism

Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3) [Complete proteome] [HAMAP]

Taxonomic identifier

70601 [NCBI]

Taxonomic lineage

cellular organisms › Archaea › Euryarchaeota › Thermococci › Thermococcales › Thermococcaceae › Pyrococcus › Pyrococcus horikoshii

Protein attributes

Sequence length	307 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Zinc phosphodiesterase, which displays some tRNA 3'-processing endonuclease activity. Probably involved in tRNA maturation, by removing a 3'-trailer from precursor tRNA By similarity. HAMAP-Rule MF_01818
Catalytic activity	Endonucleolytic cleavage of RNA, removing extra 3' nucleotides from tRNA precursor, generating 3' termini of tRNAs. A 3'-hydroxy group is left at the tRNA terminus and a 5'-phosphoryl group is left at the trailer molecule. HAMAP-Rule MF_01818
Cofactor	Binds 2 zinc ions By similarity.
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the RNase Z family.

Ontologies

Keywords
Biological process	tRNA processing
Ligand	Metal-binding Zinc
Molecular function	Endonuclease Hydrolase Nuclease
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	tRNA 3'-trailer cleavage Inferred from electronic annotation. Source: InterPro
Molecular_function	3'-tRNA processing endoribonuclease activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 307	307	Ribonuclease Z HAMAP-Rule MF_01818		PRO_0000155934
Sites
	Active site	65	1	Proton acceptor Potential
	Metal binding	61	1	Zinc 1; catalytic By similarity
	Metal binding	63	1	Zinc 1; catalytic By similarity
	Metal binding	65	1	Zinc 2; catalytic By similarity
	Metal binding	66	1	Zinc 2; catalytic By similarity
	Metal binding	138	1	Zinc 1; catalytic By similarity
	Metal binding	208	1	Zinc 1; catalytic By similarity
	Metal binding	208	1	Zinc 2; catalytic By similarity
	Metal binding	264	1	Zinc 2; catalytic By similarity

Sequences

	Sequence		Length	Mass (Da)
	O58883 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: 602F289A2739BD04	FASTA	307	35,131
	10 20 30 40 50 60 MIEVIFLGTG GIKPTPERNV PSIAIKIGGE IILFDVGEGT LRQMEIARIS PMKIKRVFIT 70 80 90 100 110 120 HFHGDHYLGL PALIQTMSLW KRKDPLHIYG PEGSSTFLQN LLNSGYFAPS FDILVHEISG 130 140 150 160 170 180 KSRLKFKEYE VWAFEVSHGV PALGYVFKEK DKRGNFNLKK IKELGLEPGP WMKELERQKI 190 200 210 220 230 240 IEINGKIVRL LEVTGPKKRG AKVVYSGDTE PCDEVIEFSR RGTLLIHEAT YVNEEDRKDS 250 260 270 280 290 300 YHTTIEEACE IWKKSKARKL VLFHRSPRYS FKEYKEKALS ICPQAIIPRD FDRIAIEGAG DVLFKIR

References

[1]

"Complete sequence and gene organization of the genome of a hyper-thermophilic archaebacterium, Pyrococcus horikoshii OT3." Kawarabayasi Y., Sawada M., Horikawa H., Haikawa Y., Hino Y., Yamamoto S., Sekine M., Baba S., Kosugi H., Hosoyama A., Nagai Y., Sakai M., Ogura K., Otsuka R., Nakazawa H., Takamiya M., Ohfuku Y., Funahashi T. , Tanaka T., Kudoh Y., Yamazaki J., Kushida N., Oguchi A., Aoki K., Yoshizawa T., Nakamura Y., Robb F.T., Horikoshi K., Masuchi Y., Shizuya H., Kikuchi H. DNA Res. 5:55-76(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000001 Genomic DNA. Translation: BAA30251.1.
PIR	A71057.
RefSeq	NP_143054.1. NC_000961.1.
3D structure databases
ProteinModelPortal	O58883.
ModBase	Search...
Protein-protein interaction databases
STRING	70601.PH1151.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	BAA30251; BAA30251; BAA30251.
GeneID	1443470.
KEGG	pho:PH1151.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1234.
HOGENOM	HOG000272419.
KO	K00784.
OMA	EGTQHQF.
ProtClustDB	PRK00055.
Enzyme and pathway databases
BioCyc	PHOR70601:GJWR-1142-MONOMER.
Family and domain databases
HAMAP	MF_01818. RNase_Z/BN.
InterPro	IPR001279. Beta-lactamas-like. IPR013471. RNase_Z. [Graphical view]
Pfam	PF00753. Lactamase_B. 1 hit. [Graphical view]
SMART	SM00849. Lactamase_B. 1 hit. [Graphical view]
TIGRFAMs	TIGR02651. RNase_Z. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RNZ_PYRHO

Accession

Primary (citable) accession number: O58883

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 16, 2004
Last sequence update:	August 1, 1998
Last modified:	May 1, 2013
This is version 75 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program