O58878 (THIE_PYRHO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Thiamine-phosphate synthase
Short name=TP synthase
Short name=TPS
EC=2.5.1.3

Alternative name(s):
Thiamine-phosphate pyrophosphorylase
Short name=TMP pyrophosphorylase
Short name=TMP-PPase

Gene names

Name:	thiE
Ordered Locus Names:	PH1156

Organism

Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3) [Complete proteome] [HAMAP]

Taxonomic identifier

70601 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Thermococci › Thermococcales › Thermococcaceae › Pyrococcus

Protein attributes

Sequence length	207 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP) By similarity. HAMAP MF_00097
Catalytic activity	2-methyl-4-amino-5-hydroxymethylpyrimidine diphosphate + 4-methyl-5-(2-phosphono-oxyethyl)thiazole = diphosphate + thiamine phosphate. HAMAP MF_00097
Cofactor	Binds 1 magnesium ion per subunit By similarity. HAMAP MF_00097
Pathway	Cofactor biosynthesis; thiamine diphosphate biosynthesis; thiamine phosphate from 4-amino-2-methyl-5-diphosphomethylpyrimidine and 4-methyl-5-(2-phosphoethyl)-thiazole: step 1/1. HAMAP MF_00097
Sequence similarities	Belongs to the thiamine-phosphate synthase family.

Ontologies

Keywords
Biological process	Thiamine biosynthesis
Ligand	Magnesium Metal-binding
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	thiamine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW thiamine-phosphate diphosphorylase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 207	207	Thiamine-phosphate synthase		PRO_0000157075
Regions
	Region	36 – 40	5	HMP-PP binding By similarity
	Region	132 – 134	3	THZ-P binding By similarity
	Region	182 – 183	2	THZ-P binding By similarity
Sites
	Metal binding	69	1	Magnesium By similarity
	Metal binding	88	1	Magnesium By similarity
	Binding site	68	1	HMP-PP By similarity
	Binding site	106	1	HMP-PP By similarity
	Binding site	135	1	HMP-PP By similarity
	Binding site	162	1	THZ-P; via amide nitrogen By similarity

Sequences

	Sequence		Length	Mass (Da)
	O58878 [UniParc]. Last modified May 30, 2000. Version 2. Checksum: AB89345245A3A379	FASTA	207	22,489
	10 20 30 40 50 60 MNFKEKLKLY IITDRRLKPE IASVKQALEG GATSIQLRIK NAPTREMYEI GKEIRKLTNE 70 80 90 100 110 120 YGALFFVDDR IDVALAVNAD GVQLGPDDMP IEIAREIAPN LIIGASVYSL EEALEAEMKG 130 140 150 160 170 180 ADYLGAGSVF PTQTKKDVKV IGIEGLREIV NAVKIPVVAI GGINLENVRE VLLTGVDGIA 190 200 VVSAVMGTED VKRATEGLRR IIEEVLG

References

[1]

"Complete sequence and gene organization of the genome of a hyper-thermophilic archaebacterium, Pyrococcus horikoshii OT3." Kawarabayasi Y., Sawada M., Horikawa H., Haikawa Y., Hino Y., Yamamoto S., Sekine M., Baba S., Kosugi H., Hosoyama A., Nagai Y., Sakai M., Ogura K., Otsuka R., Nakazawa H., Takamiya M., Ohfuku Y., Funahashi T. , Tanaka T., Kudoh Y., Yamazaki J., Kushida N., Oguchi A., Aoki K., Yoshizawa T., Nakamura Y., Robb F.T., Horikoshi K., Masuchi Y., Shizuya H., Kikuchi H. DNA Res. 5:55-76(1998) [PubMed: 9679194] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000001 Genomic DNA. Translation: BAA30256.1.
PIR	F71057.
RefSeq	NP_143058.2. NC_000961.1.
3D structure databases
ProteinModelPortal	O58878.
SMR	O58878. Positions 2-207.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBPYRT00000000904; EBPYRP00000000904; EBPYRG00000000904.
GeneID	1443476.
GenomeReviews	Gene locus PH1156 in contig BA000001_GR.
KEGG	pho:PH1156.
NMPDR	fig|70601.1.peg.1129.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000022349.
HOGENOM	HBG754477.
OMA	FIINDDV.
PhylomeDB	O58878.
ProtClustDB	PRK00043.
Enzyme and pathway databases
BioCyc	PHOR70601:PH1156-MONOMER.
Family and domain databases
HAMAP	MF_00097. TMP_synthase. [Tree]
InterPro	IPR013785. Aldolase_TIM. IPR022998. ThiaminP_synth_SF. IPR003733. TMP_synthase. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
KO	K00788.
Pfam	PF02581. TMP-TENI. 1 hit. [Graphical view]
SUPFAM	SSF51391. TMP_synthase. 1 hit.
TIGRFAMs	TIGR00693. ThiE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

THIE_PYRHO

Accession

Primary (citable) accession number: O58878

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	May 30, 2000
Last modified:	January 25, 2012
This is version 83 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program