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Protein

Hydroxyethylthiazole kinase

Gene

thiM

Organism
Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the phosphorylation of the hydroxyl group of 4-methyl-5-beta-hydroxyethylthiazole (THZ).UniRule annotation

Catalytic activityi

ATP + 4-methyl-5-(2-hydroxyethyl)thiazole = ADP + 4-methyl-5-(2-phosphonooxyethyl)thiazole.UniRule annotation

Cofactori

Mg2+UniRule annotation

Pathwayi: thiamine diphosphate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes 4-methyl-5-(2-phosphoethyl)-thiazole from 5-(2-hydroxyethyl)-4-methylthiazole.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Hydroxyethylthiazole kinase (thiM)
This subpathway is part of the pathway thiamine diphosphate biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 4-methyl-5-(2-phosphoethyl)-thiazole from 5-(2-hydroxyethyl)-4-methylthiazole, the pathway thiamine diphosphate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei43Substrate; via amide nitrogenUniRule annotation1
Binding sitei118ATPUniRule annotation1
Binding sitei165ATPUniRule annotation1
Binding sitei192Substrate; via amide nitrogenUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Transferase
Biological processThiamine biosynthesis
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciPHOR70601:G1G39-1120-MONOMER
BRENDAi2.7.1.50 7183
UniPathwayiUPA00060; UER00139

Names & Taxonomyi

Protein namesi
Recommended name:
Hydroxyethylthiazole kinaseUniRule annotation (EC:2.7.1.50UniRule annotation)
Alternative name(s):
4-methyl-5-beta-hydroxyethylthiazole kinaseUniRule annotation
Short name:
TH kinaseUniRule annotation
Short name:
Thz kinaseUniRule annotation
Gene namesi
Name:thiMUniRule annotation
Ordered Locus Names:PH1157
OrganismiPyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Taxonomic identifieri70601 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
Proteomesi
  • UP000000752 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001569721 – 265Hydroxyethylthiazole kinaseAdd BLAST265

Interactioni

Protein-protein interaction databases

STRINGi70601.PH1157

Structurei

Secondary structure

1265
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi2 – 14Combined sources13
Beta strandi17 – 21Combined sources5
Turni24 – 26Combined sources3
Helixi27 – 37Combined sources11
Beta strandi40 – 43Combined sources4
Turni47 – 49Combined sources3
Helixi50 – 56Combined sources7
Beta strandi58 – 63Combined sources6
Helixi69 – 85Combined sources17
Beta strandi89 – 92Combined sources4
Helixi100 – 112Combined sources13
Beta strandi115 – 120Combined sources6
Helixi121 – 131Combined sources11
Helixi144 – 157Combined sources14
Beta strandi160 – 172Combined sources13
Beta strandi177 – 180Combined sources4
Helixi185 – 189Combined sources5
Helixi193 – 205Combined sources13
Helixi210 – 231Combined sources22
Helixi235 – 248Combined sources14
Helixi251 – 257Combined sources7
Beta strandi260 – 263Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3HPDX-ray1.85A1-265[»]
ProteinModelPortaliO58877
SMRiO58877
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO58877

Family & Domainsi

Sequence similaritiesi

Belongs to the Thz kinase family.UniRule annotation

Phylogenomic databases

eggNOGiarCOG00019 Archaea
COG2145 LUCA
HOGENOMiHOG000114352
KOiK00878
OMAiKRPLVHN
OrthoDBiPOG093Z0A6M

Family and domain databases

CDDicd01170 THZ_kinase, 1 hit
Gene3Di3.40.1190.20, 1 hit
HAMAPiMF_00228 Thz_kinase, 1 hit
InterProiView protein in InterPro
IPR000417 Hyethyz_kinase
IPR029056 Ribokinase-like
PANTHERiPTHR44423 PTHR44423, 1 hit
PfamiView protein in Pfam
PF02110 HK, 1 hit
PIRSFiPIRSF000513 Thz_kinase, 1 hit
PRINTSiPR01099 HYETHTZKNASE
SUPFAMiSSF53613 SSF53613, 1 hit
TIGRFAMsiTIGR00694 thiM, 1 hit

Sequencei

Sequence statusi: Complete.

O58877-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKFIIEALKR VRERRPLVHN ITNFVVMNTT ANALLALGAS PVMAHAEEEL
60 70 80 90 100
EEMIRLADAV VINIGTLDSG WRRSMVKATE IANELGKPIV LDPVGAGATK
110 120 130 140 150
FRTRVSLEIL SRGVDVLKGN FGEISALLGE EGKTRGVDSL EYGEEEAKKL
160 170 180 190 200
TMNAAREFNT TVAVTGAVDY VSDGRRTFAV YNGHELLGRV TGTGCMVAAL
210 220 230 240 250
TGAFVAVTEP LKATTSALVT FGIAAEKAYE EAKYPGSFHV KLYDWLYRIN
260
ENVIRTYAKV REVEL
Length:265
Mass (Da):28,954
Last modified:August 1, 1998 - v1
Checksum:i5D38EB1846CA02BA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000001 Genomic DNA Translation: BAA30257.1
PIRiG71057
RefSeqiWP_010885242.1, NC_000961.1

Genome annotation databases

EnsemblBacteriaiBAA30257; BAA30257; BAA30257
GeneIDi1443477
KEGGipho:PH1157

Similar proteinsi

Entry informationi

Entry nameiTHIM_PYRHO
AccessioniPrimary (citable) accession number: O58877
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: August 1, 1998
Last modified: April 25, 2018
This is version 117 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
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Main funding by: National Institutes of Health