O58877 (THIM_PYRHO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Hydroxyethylthiazole kinase
EC=2.7.1.50

Alternative name(s):
4-methyl-5-beta-hydroxyethylthiazole kinase
Short name=TH kinase
Short name=Thz kinase

Gene names

Name:	thiM
Ordered Locus Names:	PH1157

Organism

Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3) [Complete proteome] [HAMAP]

Taxonomic identifier

70601 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Thermococci › Thermococcales › Thermococcaceae › Pyrococcus

Protein attributes

Sequence length	265 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	ATP + 4-methyl-5-(2-hydroxyethyl)thiazole = ADP + 4-methyl-5-(2-phosphonooxyethyl)thiazole. HAMAP MF_00228
Cofactor	Magnesium By similarity. HAMAP MF_00228
Pathway	Cofactor biosynthesis; thiamine diphosphate biosynthesis; 4-methyl-5-(2-phosphoethyl)-thiazole from 5-(2-hydroxyethyl)-4-methylthiazole: step 1/1. HAMAP MF_00228
Sequence similarities	Belongs to the Thz kinase family.

Ontologies

Keywords
Biological process	Thiamine biosynthesis
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	thiamine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW hydroxyethylthiazole kinase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 265

265

Hydroxyethylthiazole kinase HAMAP MF_00228

PRO_0000156972

Sites

Binding site

43

1

Substrate; via amide nitrogen By similarity

Binding site

118

1

ATP By similarity

Binding site

165

1

ATP By similarity

Binding site

192

1

Substrate; via amide nitrogen By similarity

Secondary structure

1

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265

Helix Strand Turn

Details...

Helix

2 – 14

13

Beta strand

17 – 21

5

Turn

24 – 26

3

Helix

27 – 37

11

Beta strand

40 – 43

4

Turn

47 – 49

3

Helix

50 – 56

7

Beta strand

58 – 63

6

Helix

69 – 85

17

Beta strand

89 – 92

4

Helix

100 – 112

13

Beta strand

115 – 120

6

Helix

121 – 131

11

Helix

144 – 157

14

Beta strand

160 – 172

13

Beta strand

177 – 180

4

Helix

185 – 189

5

Helix

193 – 205

13

Helix

210 – 231

22

Helix

235 – 248

14

Helix

251 – 257

7

Beta strand

260 – 263

4

Sequences

	Sequence		Length	Mass (Da)
	O58877 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: 5D38EB1846CA02BA	FASTA	265	28,954
	10 20 30 40 50 60 MKFIIEALKR VRERRPLVHN ITNFVVMNTT ANALLALGAS PVMAHAEEEL EEMIRLADAV 70 80 90 100 110 120 VINIGTLDSG WRRSMVKATE IANELGKPIV LDPVGAGATK FRTRVSLEIL SRGVDVLKGN 130 140 150 160 170 180 FGEISALLGE EGKTRGVDSL EYGEEEAKKL TMNAAREFNT TVAVTGAVDY VSDGRRTFAV 190 200 210 220 230 240 YNGHELLGRV TGTGCMVAAL TGAFVAVTEP LKATTSALVT FGIAAEKAYE EAKYPGSFHV 250 260 KLYDWLYRIN ENVIRTYAKV REVEL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete sequence and gene organization of the genome of a hyper-thermophilic archaebacterium, Pyrococcus horikoshii OT3." Kawarabayasi Y., Sawada M., Horikawa H., Haikawa Y., Hino Y., Yamamoto S., Sekine M., Baba S., Kosugi H., Hosoyama A., Nagai Y., Sakai M., Ogura K., Otsuka R., Nakazawa H., Takamiya M., Ohfuku Y., Funahashi T. , Tanaka T., Kudoh Y., Yamazaki J., Kushida N., Oguchi A., Aoki K., Yoshizawa T., Nakamura Y., Robb F.T., Horikoshi K., Masuchi Y., Shizuya H., Kikuchi H. DNA Res. 5:55-76(1998) [PubMed: 9679194] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3.
[2]	"Structure of hydroxyethylthiazole kinase protein from Pyrococcus horikoshii OT3." RIKEN structural genomics initiative (RSGI) Submitted (JUN-2009) to the PDB data bank Cited for: X-RAY CRYSTALLOGRAPHY (1.85 ANGSTROMS). Strain: ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

BA000001 Genomic DNA. Translation: BAA30257.1.

PIR

G71057.

RefSeq

NP_143059.1. NC_000961.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3HPD	X-ray	1.85	A	1-265	[»]

ProteinModelPortal

O58877.

SMR

O58877. Positions 1-264.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

EBPYRT00000000016; EBPYRP00000000016; EBPYRG00000000016.

GeneID

1443477.

GenomeReviews

Gene locus PH1157 in contig BA000001_GR.

KEGG

pho:PH1157.

NMPDR

fig|70601.1.peg.1130.

Organism-specific databases

CMR

Search...

Phylogenomic databases

GeneTree

EBGT00050000022541.

HOGENOM

HBG351126.

OMA

AIRGNAG.

PhylomeDB

O58877.

ProtClustDB

PRK09355.

Enzyme and pathway databases

BioCyc

PHOR70601:PH1157-MONOMER.

Family and domain databases

HAMAP

MF_00228. Thz_kinase.
[Tree]

InterPro

IPR000417. Hyethyz_kinase.
[Graphical view]

KO

K00878.

PANTHER

PTHR20857:SF14. Hyethyz_kinase. 1 hit.

Pfam

PF02110. HK. 1 hit.
[Graphical view]

PIRSF

PIRSF000513. Thz_kinase. 1 hit.

PRINTS

PR01099. HYETHTZKNASE.

TIGRFAMs

TIGR00694. ThiM. 1 hit.

ProtoNet

Search...

Entry information

Entry name

THIM_PYRHO

Accession

Primary (citable) accession number: O58877

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	August 1, 1998
Last modified:	December 14, 2011
This is version 85 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program