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O58877

- THIM_PYRHO

UniProt

O58877 - THIM_PYRHO

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Protein

Hydroxyethylthiazole kinase

Gene

thiM

Organism
Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Catalyzes the phosphorylation of the hydroxyl group of 4-methyl-5-beta-hydroxyethylthiazole (THZ).UniRule annotation

Catalytic activityi

ATP + 4-methyl-5-(2-hydroxyethyl)thiazole = ADP + 4-methyl-5-(2-phosphonooxyethyl)thiazole.UniRule annotation

Cofactori

Magnesium.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei43 – 431Substrate; via amide nitrogenUniRule annotation
Binding sitei118 – 1181ATPUniRule annotation
Binding sitei165 – 1651ATPUniRule annotation
Binding sitei192 – 1921Substrate; via amide nitrogenUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. hydroxyethylthiazole kinase activity Source: UniProtKB-HAMAP
  3. magnesium ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. thiamine biosynthetic process Source: UniProtKB-KW
  2. thiamine diphosphate biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Thiamine biosynthesis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciPHOR70601:GJWR-1149-MONOMER.
UniPathwayiUPA00060; UER00139.

Names & Taxonomyi

Protein namesi
Recommended name:
Hydroxyethylthiazole kinaseUniRule annotation (EC:2.7.1.50UniRule annotation)
Alternative name(s):
4-methyl-5-beta-hydroxyethylthiazole kinaseUniRule annotation
Short name:
TH kinaseUniRule annotation
Short name:
Thz kinaseUniRule annotation
Gene namesi
Name:thiMUniRule annotation
Ordered Locus Names:PH1157
OrganismiPyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Taxonomic identifieri70601 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
ProteomesiUP000000752: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 265265Hydroxyethylthiazole kinasePRO_0000156972Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi70601.PH1157.

Structurei

Secondary structure

1
265
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi2 – 1413
Beta strandi17 – 215
Turni24 – 263
Helixi27 – 3711
Beta strandi40 – 434
Turni47 – 493
Helixi50 – 567
Beta strandi58 – 636
Helixi69 – 8517
Beta strandi89 – 924
Helixi100 – 11213
Beta strandi115 – 1206
Helixi121 – 13111
Helixi144 – 15714
Beta strandi160 – 17213
Beta strandi177 – 1804
Helixi185 – 1895
Helixi193 – 20513
Helixi210 – 23122
Helixi235 – 24814
Helixi251 – 2577
Beta strandi260 – 2634

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3HPDX-ray1.85A1-265[»]
ProteinModelPortaliO58877.
SMRiO58877. Positions 1-264.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO58877.

Family & Domainsi

Sequence similaritiesi

Belongs to the Thz kinase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG2145.
HOGENOMiHOG000114352.
KOiK00878.
OMAiNACTQLA.

Family and domain databases

Gene3Di3.40.1190.20. 1 hit.
HAMAPiMF_00228. Thz_kinase.
InterProiIPR000417. Hyethyz_kinase.
IPR029056. Ribokinase-like.
[Graphical view]
PfamiPF02110. HK. 1 hit.
[Graphical view]
PIRSFiPIRSF000513. Thz_kinase. 1 hit.
PRINTSiPR01099. HYETHTZKNASE.
SUPFAMiSSF53613. SSF53613. 1 hit.
TIGRFAMsiTIGR00694. thiM. 1 hit.

Sequencei

Sequence statusi: Complete.

O58877-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKFIIEALKR VRERRPLVHN ITNFVVMNTT ANALLALGAS PVMAHAEEEL
60 70 80 90 100
EEMIRLADAV VINIGTLDSG WRRSMVKATE IANELGKPIV LDPVGAGATK
110 120 130 140 150
FRTRVSLEIL SRGVDVLKGN FGEISALLGE EGKTRGVDSL EYGEEEAKKL
160 170 180 190 200
TMNAAREFNT TVAVTGAVDY VSDGRRTFAV YNGHELLGRV TGTGCMVAAL
210 220 230 240 250
TGAFVAVTEP LKATTSALVT FGIAAEKAYE EAKYPGSFHV KLYDWLYRIN
260
ENVIRTYAKV REVEL
Length:265
Mass (Da):28,954
Last modified:August 1, 1998 - v1
Checksum:i5D38EB1846CA02BA
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000001 Genomic DNA. Translation: BAA30257.1.
PIRiG71057.
RefSeqiNP_143059.1. NC_000961.1.
WP_010885242.1. NC_000961.1.

Genome annotation databases

EnsemblBacteriaiBAA30257; BAA30257; BAA30257.
GeneIDi1443477.
KEGGipho:PH1157.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000001 Genomic DNA. Translation: BAA30257.1 .
PIRi G71057.
RefSeqi NP_143059.1. NC_000961.1.
WP_010885242.1. NC_000961.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
3HPD X-ray 1.85 A 1-265 [» ]
ProteinModelPortali O58877.
SMRi O58877. Positions 1-264.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 70601.PH1157.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAA30257 ; BAA30257 ; BAA30257 .
GeneIDi 1443477.
KEGGi pho:PH1157.

Phylogenomic databases

eggNOGi COG2145.
HOGENOMi HOG000114352.
KOi K00878.
OMAi NACTQLA.

Enzyme and pathway databases

UniPathwayi UPA00060 ; UER00139 .
BioCyci PHOR70601:GJWR-1149-MONOMER.

Miscellaneous databases

EvolutionaryTracei O58877.

Family and domain databases

Gene3Di 3.40.1190.20. 1 hit.
HAMAPi MF_00228. Thz_kinase.
InterProi IPR000417. Hyethyz_kinase.
IPR029056. Ribokinase-like.
[Graphical view ]
Pfami PF02110. HK. 1 hit.
[Graphical view ]
PIRSFi PIRSF000513. Thz_kinase. 1 hit.
PRINTSi PR01099. HYETHTZKNASE.
SUPFAMi SSF53613. SSF53613. 1 hit.
TIGRFAMsi TIGR00694. thiM. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3.
  2. "Structure of hydroxyethylthiazole kinase protein from Pyrococcus horikoshii OT3."
    RIKEN structural genomics initiative (RSGI)
    Submitted (JUN-2009) to the PDB data bank
    Cited for: X-RAY CRYSTALLOGRAPHY (1.85 ANGSTROMS).
    Strain: ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3.

Entry informationi

Entry nameiTHIM_PYRHO
AccessioniPrimary (citable) accession number: O58877
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: August 1, 1998
Last modified: October 29, 2014
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3