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O58843 (ATGT_PYRHO) Reviewed, UniProtKB/Swiss-Prot

Last modified November 16, 2011. Version 72. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
7-cyano-7-deazaguanine tRNA-ribosyltransferase
EC=2.4.2.-
Alternative name(s):
Archaeal tRNA-guanine transglycosylase
Gene names
Name:tgtA
Ordered Locus Names:PH1116
OrganismPyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3) [Complete proteome] [HAMAP]
Taxonomic identifier70601 [NCBI]
Taxonomic lineageArchaea › Euryarchaeota › Thermococci › Thermococcales › Thermococcaceae › Pyrococcus

Protein attributes

Sequence length582 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Exchanges the guanine residue with 7-cyano-7-deazaguanine (preQ0) at position 15 in the dihydrouridine loop (D-loop) of archaeal tRNAs. HAMAP MF_01634

Catalytic activity

tRNA guanine + 7-cyano-7-deazaguanine = tRNA 7-cyano-7-deazaguanine + guanine. HAMAP MF_01634

Cofactor

Binds 1 zinc ion per subunit.

Pathway

tRNA modification; archaeosine-tRNA biosynthesis. HAMAP MF_01634

Subunit structure

Homodimer. Ref.2

Sequence similarities

Belongs to the archaeosine tRNA-ribosyltransferase family.

Contains 1 PUA domain.

Ontologies

Keywords
   Biological processtRNA processing
   LigandMetal-binding
Zinc
   Molecular functionGlycosyltransferase
Transferase
   Technical term3D-structure
Complete proteome
Gene Ontology (GO)
   Biological processqueuosine biosynthetic process

Inferred from electronic annotation. Source: InterPro

   Molecular functionRNA binding

Inferred from electronic annotation. Source: InterPro

queuine tRNA-ribosyltransferase activity

Inferred from electronic annotation. Source: InterPro

zinc ion binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 5825827-cyano-7-deazaguanine tRNA-ribosyltransferase HAMAP MF_01634
PRO_0000247617

Regions

Domain507 – 58276PUA

Sites

Active site951Nucleophile
Metal binding2791Zinc
Metal binding2811Zinc
Metal binding2841Zinc

Experimental info

Mutagenesis951D → A: Abolishes the transferase activity. Ref.2
Mutagenesis961S → A: Weak decrease in transferase activity. Ref.2

Secondary structure

1 ............................................................................................................ 582
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
O58843 [UniParc].

Last modified August 1, 1998. Version 1.
Checksum: B96F1D5EC0D73AC3

FASTA58266,596
        10         20         30         40         50         60 
MSRGDKMLKF EIKARDGAGR IGKLEVNGKK IETPAIMPVV NPKQMVVEPK ELEKMGFEII 

        70         80         90        100        110        120 
ITNSYIIYKD EELRRKALEL GIHRMLDYNG IIEVDSGSFQ LMKYGSIEVS NREIIEFQHR 

       130        140        150        160        170        180 
IGVDIGTFLD IPTPPDAPRE QAVKELEITL SRAREAEEIK EIPMNATIQG STYTDLRRYA 

       190        200        210        220        230        240 
ARRLSSMNFE IHPIGGVVPL LESYRFRDVV DIVISSKMAL RPDRPVHLFG AGHPIVFALA 

       250        260        270        280        290        300 
VAMGVDLFDS ASYALYAKDD RYMTPEGTKR LDELDYFPCS CPVCSKYTPQ ELREMPKEER 

       310        320        330        340        350        360 
TRLLALHNLW VIKEEIKRVK QAIKEGELWR LVDERARSHP KLYSAYKRLL EHYTFLEEFE 

       370        380        390        400        410        420 
PITKKSALFK ISNESLRWPV VRRAKERAKS INERFGELVE HPIFGRVSRY LSLTYPFAQS 

       430        440        450        460        470        480 
EAEDDFKIEK PTKEDAIKYV MAIAEYQFGE GASRAFDDAK VELSKTGMPR QVKVNGKRLA 

       490        500        510        520        530        540 
TVRADDGLLT LGIEGAKRLH RVLPYPRMRV VVNKEAEPFA RKGKDVFAKF VIFADPGIRP 

       550        560        570        580 
YDEVLVVNEN DELLATGQAL LSGREMIVFQ YGRAVKVRKG VE

« Hide

References

« Hide 'large scale' references
[1]"Complete sequence and gene organization of the genome of a hyper-thermophilic archaebacterium, Pyrococcus horikoshii OT3."
Kawarabayasi Y., Sawada M., Horikawa H., Haikawa Y., Hino Y., Yamamoto S., Sekine M., Baba S., Kosugi H., Hosoyama A., Nagai Y., Sakai M., Ogura K., Otsuka R., Nakazawa H., Takamiya M., Ohfuku Y., Funahashi T. expand/collapse author list , Tanaka T., Kudoh Y., Yamazaki J., Kushida N., Oguchi A., Aoki K., Yoshizawa T., Nakamura Y., Robb F.T., Horikoshi K., Masuchi Y., Shizuya H., Kikuchi H.
DNA Res. 5:55-76(1998) [PubMed: 9679194] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3.
[2]"Crystal structure of archaeosine tRNA-guanine transglycosylase."
Ishitani R., Nureki O., Fukai S., Kijimoto T., Nameki N., Watanabe M., Kondo H., Sekine M., Okada N., Nishimura S., Yokoyama S.
J. Mol. Biol. 318:665-677(2002) [PubMed: 12054814] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS) IN COMPLEX WITH GUANINE AND WITH 7-CYANO-7-DEAZAGUANINE, SUBUNIT, MUTAGENESIS OF ASP-95 AND SER-96.
[3]"Alternative tertiary structure of tRNA for recognition by a posttranscriptional modification enzyme."
Ishitani R., Nureki O., Nameki N., Okada N., Nishimura S., Yokoyama S.
Cell 113:383-394(2003) [PubMed: 12732145] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (3.3 ANGSTROMS) IN COMPLEX WITH TRANSFER RNA.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		BA000001 Genomic DNA. Translation: BAA30215.1.
PIRE71052.
RefSeqNP_143020.1. NC_000961.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1IQ8X-ray2.20A/B1-582[»]
1IT7X-ray2.30A/B1-582[»]
1IT8X-ray2.50A/B1-582[»]
1J2BX-ray3.30A/B1-582[»]
ProteinModelPortalO58843.
SMRO58843. Positions 6-582.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaEBPYRT00000000145; EBPYRP00000000145; EBPYRG00000000145.
GeneID1443435.
GenomeReviewsGene locus PH1116 in contig BA000001_GR.
KEGGpho:PH1116.
NMPDRfig|70601.1.peg.1088.

Organism-specific databases

CMRSearch...

Phylogenomic databases

GeneTreeEBGT00050000022193.
HOGENOMHBG512800.
OMAIMTDSGT.
ProtClustDBPRK13534.

Enzyme and pathway databases

BioCycPHOR70601:PH1116-MONOMER.

Family and domain databases

HAMAPMF_01634. TgtA_arch.
[Tree]
InterProIPR004804. Arc_tRNA_RibTrfase.
IPR002478. PUA.
IPR015947. PUA-like_domain.
IPR002616. tRNA_ribo_trans.
IPR004521. Uncharacterised_CHP00451.
[Graphical view]
Gene3DG3DSA:3.20.20.105. tRNA_ribo_trans. 1 hit.
KOK00773.
K07398.
PANTHERPTHR11962. tRNA_ribo_trans. 1 hit.
PfamPF01472. PUA. 1 hit.
PF01702. TGT. 1 hit.
[Graphical view]
SMARTSM00359. PUA. 1 hit.
[Graphical view]
SUPFAMSSF88697. PUA-like. 1 hit.
SSF51713. tRNA_ribo_trans. 1 hit.
TIGRFAMsTIGR00432. Arcsn_tRNA_tgt. 1 hit.
TIGR00449. Tgt_general. 1 hit.
TIGR00451. Unchar_dom_2. 1 hit.
PROSITEPS50890. PUA. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameATGT_PYRHO
AccessionPrimary (citable) accession number: O58843
Entry history
Integrated into UniProtKB/Swiss-Prot: July 25, 2006
Last sequence update: August 1, 1998
Last modified: November 16, 2011
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

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