Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

tRNA-guanine(15) transglycosylase

Gene

tgtA

Organism
Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Exchanges the guanine residue with 7-cyano-7-deazaguanine (preQ0) at position 15 in the dihydrouridine loop (D-loop) of archaeal tRNAs.1 Publication

Catalytic activityi

Guanine(15) in tRNA + 7-cyano-7-carbaguanine = 7-cyano-7-carbaguanine(15) in tRNA + guanine.

Cofactori

Zn2+2 PublicationsNote: Binds 1 zinc ion per subunit.2 Publications

Pathwayi: archaeosine-tRNA biosynthesis

This protein is involved in the pathway archaeosine-tRNA biosynthesis, which is part of tRNA modification.
View all proteins of this organism that are known to be involved in the pathway archaeosine-tRNA biosynthesis and in tRNA modification.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei95 – 951Nucleophile1 Publication
Binding sitei130 – 1301Substrate1 Publication
Binding sitei196 – 1961Substrate; via amide nitrogen1 Publication
Metal bindingi279 – 2791Zinc2 Publications
Metal bindingi281 – 2811Zinc2 Publications
Metal bindingi284 – 2841Zinc2 Publications

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciPHOR70601:GJWR-1106-MONOMER.
BRENDAi2.4.2.48. 5244.
UniPathwayiUPA00393.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA-guanine(15) transglycosylase (EC:2.4.2.48)
Alternative name(s):
7-cyano-7-deazaguanine tRNA-ribosyltransferase
Archaeal tRNA-guanine transglycosylase
Gene namesi
Name:tgtA
Ordered Locus Names:PH1116
OrganismiPyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Taxonomic identifieri70601 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
Proteomesi
  • UP000000752 Componenti: Chromosome

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi95 – 951D → A: Abolishes the transferase activity. 1 Publication
Mutagenesisi96 – 961S → A: Weak decrease in transferase activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 582582tRNA-guanine(15) transglycosylasePRO_0000247617Add
BLAST

Interactioni

Subunit structurei

Homodimer.2 Publications

Protein-protein interaction databases

STRINGi70601.PH1116.

Structurei

Secondary structure

1
582
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi8 – 169Combined sources
Beta strandi19 – 268Combined sources
Beta strandi29 – 3911Combined sources
Beta strandi42 – 443Combined sources
Helixi49 – 546Combined sources
Beta strandi59 – 635Combined sources
Helixi64 – 696Combined sources
Helixi71 – 8616Combined sources
Beta strandi90 – 956Combined sources
Helixi100 – 1034Combined sources
Helixi111 – 12010Combined sources
Beta strandi124 – 1263Combined sources
Helixi139 – 15921Combined sources
Helixi174 – 18613Combined sources
Beta strandi190 – 1945Combined sources
Helixi198 – 2025Combined sources
Helixi206 – 21914Combined sources
Beta strandi226 – 2283Combined sources
Helixi234 – 2363Combined sources
Helixi237 – 2426Combined sources
Beta strandi247 – 2515Combined sources
Helixi252 – 2587Combined sources
Beta strandi261 – 2644Combined sources
Beta strandi267 – 2704Combined sources
Helixi271 – 2733Combined sources
Turni282 – 2865Combined sources
Helixi289 – 2924Combined sources
Helixi297 – 32529Combined sources
Helixi328 – 3369Combined sources
Helixi340 – 35112Combined sources
Helixi353 – 3597Combined sources
Helixi374 – 3774Combined sources
Helixi379 – 39517Combined sources
Beta strandi398 – 4014Combined sources
Turni402 – 4043Combined sources
Beta strandi405 – 4084Combined sources
Helixi409 – 4113Combined sources
Turni415 – 4173Combined sources
Beta strandi418 – 4214Combined sources
Turni433 – 4353Combined sources
Helixi436 – 44813Combined sources
Helixi452 – 4554Combined sources
Turni456 – 4583Combined sources
Beta strandi460 – 4634Combined sources
Beta strandi465 – 4673Combined sources
Beta strandi469 – 4735Combined sources
Beta strandi475 – 4828Combined sources
Turni484 – 4863Combined sources
Beta strandi489 – 4913Combined sources
Helixi493 – 50210Combined sources
Turni505 – 5084Combined sources
Beta strandi509 – 5124Combined sources
Turni514 – 5163Combined sources
Helixi517 – 5215Combined sources
Helixi528 – 5303Combined sources
Beta strandi531 – 5344Combined sources
Beta strandi543 – 5475Combined sources
Beta strandi553 – 5619Combined sources
Helixi563 – 5686Combined sources
Beta strandi571 – 58010Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1IQ8X-ray2.20A/B1-582[»]
1IT7X-ray2.30A/B1-582[»]
1IT8X-ray2.50A/B1-582[»]
1J2BX-ray3.30A/B1-582[»]
ProteinModelPortaliO58843.
SMRiO58843. Positions 6-582.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO58843.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini507 – 58276PUAAdd
BLAST

Sequence similaritiesi

Contains 1 PUA domain.Curated

Phylogenomic databases

eggNOGiarCOG00989. Archaea.
COG0343. LUCA.
COG1370. LUCA.
HOGENOMiHOG000231009.
KOiK18779.
OMAiAFFYTGP.

Family and domain databases

Gene3Di2.30.130.10. 1 hit.
3.20.20.105. 1 hit.
HAMAPiMF_01634. TgtA_arch.
InterProiIPR002478. PUA.
IPR015947. PUA-like_domain.
IPR032729. TGT_C1.
IPR029402. TGT_C2.
IPR004804. TgtA.
IPR002616. tRNA_ribo_trans-like.
IPR004521. Uncharacterised_CHP00451.
[Graphical view]
PfamiPF01472. PUA. 1 hit.
PF01702. TGT. 1 hit.
PF14809. TGT_C1. 1 hit.
PF14810. TGT_C2. 1 hit.
[Graphical view]
SMARTiSM00359. PUA. 1 hit.
[Graphical view]
SUPFAMiSSF51713. SSF51713. 1 hit.
SSF88697. SSF88697. 1 hit.
TIGRFAMsiTIGR00432. arcsn_tRNA_tgt. 1 hit.
TIGR00449. tgt_general. 1 hit.
TIGR00451. unchar_dom_2. 1 hit.
PROSITEiPS50890. PUA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O58843-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSRGDKMLKF EIKARDGAGR IGKLEVNGKK IETPAIMPVV NPKQMVVEPK
60 70 80 90 100
ELEKMGFEII ITNSYIIYKD EELRRKALEL GIHRMLDYNG IIEVDSGSFQ
110 120 130 140 150
LMKYGSIEVS NREIIEFQHR IGVDIGTFLD IPTPPDAPRE QAVKELEITL
160 170 180 190 200
SRAREAEEIK EIPMNATIQG STYTDLRRYA ARRLSSMNFE IHPIGGVVPL
210 220 230 240 250
LESYRFRDVV DIVISSKMAL RPDRPVHLFG AGHPIVFALA VAMGVDLFDS
260 270 280 290 300
ASYALYAKDD RYMTPEGTKR LDELDYFPCS CPVCSKYTPQ ELREMPKEER
310 320 330 340 350
TRLLALHNLW VIKEEIKRVK QAIKEGELWR LVDERARSHP KLYSAYKRLL
360 370 380 390 400
EHYTFLEEFE PITKKSALFK ISNESLRWPV VRRAKERAKS INERFGELVE
410 420 430 440 450
HPIFGRVSRY LSLTYPFAQS EAEDDFKIEK PTKEDAIKYV MAIAEYQFGE
460 470 480 490 500
GASRAFDDAK VELSKTGMPR QVKVNGKRLA TVRADDGLLT LGIEGAKRLH
510 520 530 540 550
RVLPYPRMRV VVNKEAEPFA RKGKDVFAKF VIFADPGIRP YDEVLVVNEN
560 570 580
DELLATGQAL LSGREMIVFQ YGRAVKVRKG VE
Length:582
Mass (Da):66,596
Last modified:August 1, 1998 - v1
Checksum:iB96F1D5EC0D73AC3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000001 Genomic DNA. Translation: BAA30215.1.
PIRiE71052.
RefSeqiWP_010885200.1. NC_000961.1.

Genome annotation databases

EnsemblBacteriaiBAA30215; BAA30215; BAA30215.
GeneIDi1443435.
KEGGipho:PH1116.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000001 Genomic DNA. Translation: BAA30215.1.
PIRiE71052.
RefSeqiWP_010885200.1. NC_000961.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1IQ8X-ray2.20A/B1-582[»]
1IT7X-ray2.30A/B1-582[»]
1IT8X-ray2.50A/B1-582[»]
1J2BX-ray3.30A/B1-582[»]
ProteinModelPortaliO58843.
SMRiO58843. Positions 6-582.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi70601.PH1116.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAA30215; BAA30215; BAA30215.
GeneIDi1443435.
KEGGipho:PH1116.

Phylogenomic databases

eggNOGiarCOG00989. Archaea.
COG0343. LUCA.
COG1370. LUCA.
HOGENOMiHOG000231009.
KOiK18779.
OMAiAFFYTGP.

Enzyme and pathway databases

UniPathwayiUPA00393.
BioCyciPHOR70601:GJWR-1106-MONOMER.
BRENDAi2.4.2.48. 5244.

Miscellaneous databases

EvolutionaryTraceiO58843.

Family and domain databases

Gene3Di2.30.130.10. 1 hit.
3.20.20.105. 1 hit.
HAMAPiMF_01634. TgtA_arch.
InterProiIPR002478. PUA.
IPR015947. PUA-like_domain.
IPR032729. TGT_C1.
IPR029402. TGT_C2.
IPR004804. TgtA.
IPR002616. tRNA_ribo_trans-like.
IPR004521. Uncharacterised_CHP00451.
[Graphical view]
PfamiPF01472. PUA. 1 hit.
PF01702. TGT. 1 hit.
PF14809. TGT_C1. 1 hit.
PF14810. TGT_C2. 1 hit.
[Graphical view]
SMARTiSM00359. PUA. 1 hit.
[Graphical view]
SUPFAMiSSF51713. SSF51713. 1 hit.
SSF88697. SSF88697. 1 hit.
TIGRFAMsiTIGR00432. arcsn_tRNA_tgt. 1 hit.
TIGR00449. tgt_general. 1 hit.
TIGR00451. unchar_dom_2. 1 hit.
PROSITEiPS50890. PUA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3.
  2. Cited for: X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS) IN COMPLEX WITH SUBSTRATE ANALOG AND ZINC ION, FUNCTION, COFACTOR, SUBUNIT, MUTAGENESIS OF ASP-95 AND SER-96, ACTIVE SITE.
  3. "Alternative tertiary structure of tRNA for recognition by a posttranscriptional modification enzyme."
    Ishitani R., Nureki O., Nameki N., Okada N., Nishimura S., Yokoyama S.
    Cell 113:383-394(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (3.3 ANGSTROMS) IN COMPLEX WITH ZINC ION, COFACTOR, SUBUNIT.

Entry informationi

Entry nameiATGT_PYRHO
AccessioniPrimary (citable) accession number: O58843
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 25, 2006
Last sequence update: August 1, 1998
Last modified: January 20, 2016
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.