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Protein

tRNA-guanine(15) transglycosylase

Gene

tgtA

Organism
Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Exchanges the guanine residue with 7-cyano-7-deazaguanine (preQ0) at position 15 in the dihydrouridine loop (D-loop) of archaeal tRNAs.1 Publication

Catalytic activityi

Guanine(15) in tRNA + 7-cyano-7-carbaguanine = 7-cyano-7-carbaguanine(15) in tRNA + guanine.

Cofactori

Zn2+2 PublicationsNote: Binds 1 zinc ion per subunit.2 Publications

Pathwayi: archaeosine-tRNA biosynthesis

This protein is involved in the pathway archaeosine-tRNA biosynthesis, which is part of tRNA modification.
View all proteins of this organism that are known to be involved in the pathway archaeosine-tRNA biosynthesis and in tRNA modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei95Nucleophile1 Publication1
Binding sitei130Substrate1 Publication1
Binding sitei196Substrate; via amide nitrogen1 Publication1
Metal bindingi279Zinc2 Publications1
Metal bindingi281Zinc2 Publications1
Metal bindingi284Zinc2 Publications1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BRENDAi2.4.2.48. 5244.
UniPathwayiUPA00393.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA-guanine(15) transglycosylase (EC:2.4.2.48)
Alternative name(s):
7-cyano-7-deazaguanine tRNA-ribosyltransferase
Archaeal tRNA-guanine transglycosylase
Gene namesi
Name:tgtA
Ordered Locus Names:PH1116
OrganismiPyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Taxonomic identifieri70601 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
Proteomesi
  • UP000000752 Componenti: Chromosome

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi95D → A: Abolishes the transferase activity. 1 Publication1
Mutagenesisi96S → A: Weak decrease in transferase activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002476171 – 582tRNA-guanine(15) transglycosylaseAdd BLAST582

Interactioni

Subunit structurei

Homodimer.2 Publications

Protein-protein interaction databases

STRINGi70601.PH1116.

Structurei

Secondary structure

1582
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi8 – 16Combined sources9
Beta strandi19 – 26Combined sources8
Beta strandi29 – 39Combined sources11
Beta strandi42 – 44Combined sources3
Helixi49 – 54Combined sources6
Beta strandi59 – 63Combined sources5
Helixi64 – 69Combined sources6
Helixi71 – 86Combined sources16
Beta strandi90 – 95Combined sources6
Helixi100 – 103Combined sources4
Helixi111 – 120Combined sources10
Beta strandi124 – 126Combined sources3
Helixi139 – 159Combined sources21
Helixi174 – 186Combined sources13
Beta strandi190 – 194Combined sources5
Helixi198 – 202Combined sources5
Helixi206 – 219Combined sources14
Beta strandi226 – 228Combined sources3
Helixi234 – 236Combined sources3
Helixi237 – 242Combined sources6
Beta strandi247 – 251Combined sources5
Helixi252 – 258Combined sources7
Beta strandi261 – 264Combined sources4
Beta strandi267 – 270Combined sources4
Helixi271 – 273Combined sources3
Turni282 – 286Combined sources5
Helixi289 – 292Combined sources4
Helixi297 – 325Combined sources29
Helixi328 – 336Combined sources9
Helixi340 – 351Combined sources12
Helixi353 – 359Combined sources7
Helixi374 – 377Combined sources4
Helixi379 – 395Combined sources17
Beta strandi398 – 401Combined sources4
Turni402 – 404Combined sources3
Beta strandi405 – 408Combined sources4
Helixi409 – 411Combined sources3
Turni415 – 417Combined sources3
Beta strandi418 – 421Combined sources4
Turni433 – 435Combined sources3
Helixi436 – 448Combined sources13
Helixi452 – 455Combined sources4
Turni456 – 458Combined sources3
Beta strandi460 – 463Combined sources4
Beta strandi465 – 467Combined sources3
Beta strandi469 – 473Combined sources5
Beta strandi475 – 482Combined sources8
Turni484 – 486Combined sources3
Beta strandi489 – 491Combined sources3
Helixi493 – 502Combined sources10
Turni505 – 508Combined sources4
Beta strandi509 – 512Combined sources4
Turni514 – 516Combined sources3
Helixi517 – 521Combined sources5
Helixi528 – 530Combined sources3
Beta strandi531 – 534Combined sources4
Beta strandi543 – 547Combined sources5
Beta strandi553 – 561Combined sources9
Helixi563 – 568Combined sources6
Beta strandi571 – 580Combined sources10

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1IQ8X-ray2.20A/B1-582[»]
1IT7X-ray2.30A/B1-582[»]
1IT8X-ray2.50A/B1-582[»]
1J2BX-ray3.30A/B1-582[»]
ProteinModelPortaliO58843.
SMRiO58843.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO58843.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini507 – 582PUAAdd BLAST76

Sequence similaritiesi

Contains 1 PUA domain.Curated

Phylogenomic databases

eggNOGiarCOG00989. Archaea.
COG0343. LUCA.
COG1370. LUCA.
HOGENOMiHOG000231009.
KOiK18779.
OMAiAFFYTGP.

Family and domain databases

Gene3Di2.30.130.10. 1 hit.
3.20.20.105. 1 hit.
HAMAPiMF_01634. TgtA_arch. 1 hit.
InterProiIPR002478. PUA.
IPR015947. PUA-like_domain.
IPR032729. TGT_C1.
IPR029402. TGT_C2.
IPR004804. TgtA.
IPR002616. tRNA_ribo_trans-like.
IPR004521. Uncharacterised_CHP00451.
[Graphical view]
PfamiPF01472. PUA. 1 hit.
PF01702. TGT. 1 hit.
PF14809. TGT_C1. 1 hit.
PF14810. TGT_C2. 1 hit.
[Graphical view]
SMARTiSM00359. PUA. 1 hit.
[Graphical view]
SUPFAMiSSF51713. SSF51713. 1 hit.
SSF88697. SSF88697. 1 hit.
TIGRFAMsiTIGR00432. arcsn_tRNA_tgt. 1 hit.
TIGR00449. tgt_general. 1 hit.
TIGR00451. unchar_dom_2. 1 hit.
PROSITEiPS50890. PUA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O58843-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSRGDKMLKF EIKARDGAGR IGKLEVNGKK IETPAIMPVV NPKQMVVEPK
60 70 80 90 100
ELEKMGFEII ITNSYIIYKD EELRRKALEL GIHRMLDYNG IIEVDSGSFQ
110 120 130 140 150
LMKYGSIEVS NREIIEFQHR IGVDIGTFLD IPTPPDAPRE QAVKELEITL
160 170 180 190 200
SRAREAEEIK EIPMNATIQG STYTDLRRYA ARRLSSMNFE IHPIGGVVPL
210 220 230 240 250
LESYRFRDVV DIVISSKMAL RPDRPVHLFG AGHPIVFALA VAMGVDLFDS
260 270 280 290 300
ASYALYAKDD RYMTPEGTKR LDELDYFPCS CPVCSKYTPQ ELREMPKEER
310 320 330 340 350
TRLLALHNLW VIKEEIKRVK QAIKEGELWR LVDERARSHP KLYSAYKRLL
360 370 380 390 400
EHYTFLEEFE PITKKSALFK ISNESLRWPV VRRAKERAKS INERFGELVE
410 420 430 440 450
HPIFGRVSRY LSLTYPFAQS EAEDDFKIEK PTKEDAIKYV MAIAEYQFGE
460 470 480 490 500
GASRAFDDAK VELSKTGMPR QVKVNGKRLA TVRADDGLLT LGIEGAKRLH
510 520 530 540 550
RVLPYPRMRV VVNKEAEPFA RKGKDVFAKF VIFADPGIRP YDEVLVVNEN
560 570 580
DELLATGQAL LSGREMIVFQ YGRAVKVRKG VE
Length:582
Mass (Da):66,596
Last modified:August 1, 1998 - v1
Checksum:iB96F1D5EC0D73AC3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000001 Genomic DNA. Translation: BAA30215.1.
PIRiE71052.
RefSeqiWP_010885200.1. NC_000961.1.

Genome annotation databases

EnsemblBacteriaiBAA30215; BAA30215; BAA30215.
GeneIDi1443435.
KEGGipho:PH1116.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000001 Genomic DNA. Translation: BAA30215.1.
PIRiE71052.
RefSeqiWP_010885200.1. NC_000961.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1IQ8X-ray2.20A/B1-582[»]
1IT7X-ray2.30A/B1-582[»]
1IT8X-ray2.50A/B1-582[»]
1J2BX-ray3.30A/B1-582[»]
ProteinModelPortaliO58843.
SMRiO58843.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi70601.PH1116.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAA30215; BAA30215; BAA30215.
GeneIDi1443435.
KEGGipho:PH1116.

Phylogenomic databases

eggNOGiarCOG00989. Archaea.
COG0343. LUCA.
COG1370. LUCA.
HOGENOMiHOG000231009.
KOiK18779.
OMAiAFFYTGP.

Enzyme and pathway databases

UniPathwayiUPA00393.
BRENDAi2.4.2.48. 5244.

Miscellaneous databases

EvolutionaryTraceiO58843.

Family and domain databases

Gene3Di2.30.130.10. 1 hit.
3.20.20.105. 1 hit.
HAMAPiMF_01634. TgtA_arch. 1 hit.
InterProiIPR002478. PUA.
IPR015947. PUA-like_domain.
IPR032729. TGT_C1.
IPR029402. TGT_C2.
IPR004804. TgtA.
IPR002616. tRNA_ribo_trans-like.
IPR004521. Uncharacterised_CHP00451.
[Graphical view]
PfamiPF01472. PUA. 1 hit.
PF01702. TGT. 1 hit.
PF14809. TGT_C1. 1 hit.
PF14810. TGT_C2. 1 hit.
[Graphical view]
SMARTiSM00359. PUA. 1 hit.
[Graphical view]
SUPFAMiSSF51713. SSF51713. 1 hit.
SSF88697. SSF88697. 1 hit.
TIGRFAMsiTIGR00432. arcsn_tRNA_tgt. 1 hit.
TIGR00449. tgt_general. 1 hit.
TIGR00451. unchar_dom_2. 1 hit.
PROSITEiPS50890. PUA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiATGT_PYRHO
AccessioniPrimary (citable) accession number: O58843
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 25, 2006
Last sequence update: August 1, 1998
Last modified: November 2, 2016
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.