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Protein

Probable translation initiation factor IF-2

Gene

infB

Organism
Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Function in general translation initiation by promoting the binding of the formylmethionine-tRNA to ribosomes. Seems to function along with eIF-2 (By similarity).By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi524 – 528GTPBy similarity5
Nucleotide bindingi578 – 581GTPBy similarity4

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Initiation factor, Nuclease

Keywords - Biological processi

Intron homing, Protein biosynthesis

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Protein family/group databases

MEROPSiN10.004.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable translation initiation factor IF-2
Cleaved into the following chain:
Alternative name(s):
Pho IF2 intein
Gene namesi
Name:infB
Ordered Locus Names:PH1095
OrganismiPyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Taxonomic identifieri70601 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
Proteomesi
  • UP000000752 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000144931 – 22Probable translation initiation factor IF-2, 1st partSequence analysisAdd BLAST22
ChainiPRO_000001449423 – 466Pho infB inteinSequence analysisAdd BLAST444
ChainiPRO_0000014495467 – 1044Probable translation initiation factor IF-2, 2nd partSequence analysisAdd BLAST578

Post-translational modificationi

This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation.Curated

Keywords - PTMi

Autocatalytic cleavage, Protein splicing

Interactioni

Protein-protein interaction databases

STRINGi70601.PH1095.

Structurei

3D structure databases

ProteinModelPortaliO58822.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini173 – 265DOD-type homing endonucleaseAdd BLAST93
Domaini451 – 668tr-type GAdd BLAST218

Sequence similaritiesi

Phylogenomic databases

eggNOGiarCOG01560. Archaea.
arCOG03151. Archaea.
COG0532. LUCA.
HOGENOMiHOG000105770.
KOiK03243.
OMAiDHGKCLL.

Family and domain databases

Gene3Di2.170.16.10. 2 hits.
3.10.28.10. 1 hit.
3.40.50.10050. 1 hit.
3.40.50.300. 1 hit.
HAMAPiMF_00100_A. IF_2_A. 1 hit.
InterProiIPR004161. EFTu-like_2.
IPR029459. EFTU-type.
IPR028992. Hedgehog/Intein_dom.
IPR003586. Hint_dom_C.
IPR003587. Hint_dom_N.
IPR027434. Homing_endonucl.
IPR006142. INTEIN.
IPR030934. Intein_C.
IPR004042. Intein_endonuc.
IPR006141. Intein_N.
IPR004860. LAGLIDADG_2.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR000795. TF_GTP-bd_dom.
IPR004544. TIF_aIF-2_arc.
IPR023115. TIF_IF2_dom3.
IPR009000. Transl_B-barrel.
[Graphical view]
PfamiPF03144. GTP_EFTU_D2. 1 hit.
PF14578. GTP_EFTU_D4. 1 hit.
PF11987. IF-2. 1 hit.
PF14528. LAGLIDADG_3. 1 hit.
[Graphical view]
PRINTSiPR00379. INTEIN.
SMARTiSM00305. HintC. 1 hit.
SM00306. HintN. 1 hit.
[Graphical view]
SUPFAMiSSF50447. SSF50447. 1 hit.
SSF51294. SSF51294. 2 hits.
SSF52156. SSF52156. 1 hit.
SSF52540. SSF52540. 1 hit.
SSF55608. SSF55608. 1 hit.
TIGRFAMsiTIGR00491. aIF-2. 1 hit.
TIGR01443. intein_Cterm. 1 hit.
TIGR01445. intein_Nterm. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEiPS51722. G_TR_2. 1 hit.
PS50818. INTEIN_C_TER. 1 hit.
PS50819. INTEIN_ENDONUCLEASE. 1 hit.
PS50817. INTEIN_N_TER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O58822-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSYVKRIRQP IIAVLGHVDH GKCLLPEERV ILPDYGPITL EELFNMTKET
60 70 80 90 100
VFKDEEKEVR KLGIRMPVAG VDGRVRLLEG PYVWKVRYKG KMLRVKLKDW
110 120 130 140 150
HSVAVTPEHP FLTTRGWVRA DQLKPGDYVA VPKILPGKDD KEKFLQYVHE
160 170 180 190 200
KLKGKVHIKL PSSDEEWETF FYFAGTIFGR ENSVNPEGLT HEVKALLELF
210 220 230 240 250
KVLFEYPREV LRVLFMAPVR YVANFLRGFF DINGYVNGEE LRVEVRGAPH
260 270 280 290 300
EVLEELSLIL LRLGIVSKIY PTSLAISGRR NLELFRRYIG FSEKQKAKEL
310 320 330 340 350
EGIIRRSENS ESYPIFEELR RIRLLFGFTR AELSSTIPLY SKYESKEAPS
360 370 380 390 400
YEILMKILNT IEKGSKDLNK KITILEGRVR DHEYIEEFKR EGLIKDGKLT
410 420 430 440 450
ELGKELLEVW RNREFDSRDV NYLRNIIENF VFLPVEKIEE FEYDGYVYDV
460 470 480 490 500
TTETHNFIAN GILVHNTTLL DKIRKTNVAA KEAGGITQHI GATEVPIDVV
510 520 530 540 550
KKIAGPLIKL WKAEIRLPGL LFIDTPGHEA FTSLRARGGS LADLAVLVID
560 570 580 590 600
VNEGFQPQTI ESIEILRRYR TPFVVAANKI DRIRGWVIEE DEPFLMNIKR
610 620 630 640 650
QDQRAIQELE TKLWELIGKF YEFGFQANRF DRVQNFTREL AIVPISAKYG
660 670 680 690 700
IGIAELLVLI AGLSQKYLEE KLKIEVEGPA RGTILEVREE PGLGHTIDVI
710 720 730 740 750
IYDGTLHKDD TIVVGGKDKA IVTKVRALLK PKPLDEIRDP RFRFDYVDEV
760 770 780 790 800
TAAAGVKIAA PGLEEALAGS PVIAAPTPED VERAKEEIMR QIESVVISTD
810 820 830 840 850
KVGVIVKADT LGSLEALSKE LQEKEIPIRK ADVGNISKTD VMEALSVKEE
860 870 880 890 900
NPKYGVILGF NVKVNEDAKE VAKAKEVPIF VGNIIYKLIE DYEAWIKEEE
910 920 930 940 950
EKRKRELLAK VTFPGVIKLY PDERYVFRRS NPAIVGIEVL EGRIKPGVTL
960 970 980 990 1000
IKQNGQKVGT IRSIKSRDEF LQEARKGQAV AIAIEGAIVG RHIHPGETLY
1010 1020 1030 1040
VDLSRDDAII LLKHLRDVLE DTDIKALKMI AQVKAKEDPF WRAV
Length:1,044
Mass (Da):118,951
Last modified:August 1, 1998 - v1
Checksum:i907C7A84D0ACB3F4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000001 Genomic DNA. Translation: BAA30194.1.
PIRiH71049.

Genome annotation databases

EnsemblBacteriaiBAA30194; BAA30194; BAA30194.
KEGGipho:PH1095.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000001 Genomic DNA. Translation: BAA30194.1.
PIRiH71049.

3D structure databases

ProteinModelPortaliO58822.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi70601.PH1095.

Protein family/group databases

MEROPSiN10.004.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAA30194; BAA30194; BAA30194.
KEGGipho:PH1095.

Phylogenomic databases

eggNOGiarCOG01560. Archaea.
arCOG03151. Archaea.
COG0532. LUCA.
HOGENOMiHOG000105770.
KOiK03243.
OMAiDHGKCLL.

Family and domain databases

Gene3Di2.170.16.10. 2 hits.
3.10.28.10. 1 hit.
3.40.50.10050. 1 hit.
3.40.50.300. 1 hit.
HAMAPiMF_00100_A. IF_2_A. 1 hit.
InterProiIPR004161. EFTu-like_2.
IPR029459. EFTU-type.
IPR028992. Hedgehog/Intein_dom.
IPR003586. Hint_dom_C.
IPR003587. Hint_dom_N.
IPR027434. Homing_endonucl.
IPR006142. INTEIN.
IPR030934. Intein_C.
IPR004042. Intein_endonuc.
IPR006141. Intein_N.
IPR004860. LAGLIDADG_2.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR000795. TF_GTP-bd_dom.
IPR004544. TIF_aIF-2_arc.
IPR023115. TIF_IF2_dom3.
IPR009000. Transl_B-barrel.
[Graphical view]
PfamiPF03144. GTP_EFTU_D2. 1 hit.
PF14578. GTP_EFTU_D4. 1 hit.
PF11987. IF-2. 1 hit.
PF14528. LAGLIDADG_3. 1 hit.
[Graphical view]
PRINTSiPR00379. INTEIN.
SMARTiSM00305. HintC. 1 hit.
SM00306. HintN. 1 hit.
[Graphical view]
SUPFAMiSSF50447. SSF50447. 1 hit.
SSF51294. SSF51294. 2 hits.
SSF52156. SSF52156. 1 hit.
SSF52540. SSF52540. 1 hit.
SSF55608. SSF55608. 1 hit.
TIGRFAMsiTIGR00491. aIF-2. 1 hit.
TIGR01443. intein_Cterm. 1 hit.
TIGR01445. intein_Nterm. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEiPS51722. G_TR_2. 1 hit.
PS50818. INTEIN_C_TER. 1 hit.
PS50819. INTEIN_ENDONUCLEASE. 1 hit.
PS50817. INTEIN_N_TER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiIF2P_PYRHO
AccessioniPrimary (citable) accession number: O58822
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: August 1, 1998
Last modified: November 2, 2016
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

The intein interrupts the GTP-binding site.

Keywords - Technical termi

Complete proteome

Documents

  1. Intein-containing proteins
    List of intein-containing protein entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.