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O58822 (IF2P_PYRHO) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 101. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Probable translation initiation factor IF-2

Cleaved into the following chain:

  1. Pho infB intein
    Alternative name(s):
    Pho IF2 intein
Gene names
Name:infB
Ordered Locus Names:PH1095
OrganismPyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3) [Complete proteome] [HAMAP]
Taxonomic identifier70601 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus

Protein attributes

Sequence length1044 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Function in general translation initiation by promoting the binding of the formylmethionine-tRNA to ribosomes. Seems to function along with eIF-2 By similarity. HAMAP-Rule MF_00100_A

Post-translational modification

This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation Potential. HAMAP-Rule MF_00100_A

Miscellaneous

The intein interrupts the GTP-binding site.

Sequence similarities

Belongs to the IF-2 family.

Contains 1 DOD-type homing endonuclease domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 2222Probable translation initiation factor IF-2, 1st part Potential
PRO_0000014493
Chain23 – 466444Pho infB intein Potential
PRO_0000014494
Chain467 – 1044578Probable translation initiation factor IF-2, 2nd part Potential
PRO_0000014495

Regions

Domain173 – 26593DOD-type homing endonuclease
Nucleotide binding524 – 5285GTP By similarity
Nucleotide binding578 – 5814GTP By similarity

Sequences

Sequence LengthMass (Da)Tools
O58822 [UniParc].

Last modified August 1, 1998. Version 1.
Checksum: 907C7A84D0ACB3F4

FASTA1,044118,951
        10         20         30         40         50         60 
MSYVKRIRQP IIAVLGHVDH GKCLLPEERV ILPDYGPITL EELFNMTKET VFKDEEKEVR 

        70         80         90        100        110        120 
KLGIRMPVAG VDGRVRLLEG PYVWKVRYKG KMLRVKLKDW HSVAVTPEHP FLTTRGWVRA 

       130        140        150        160        170        180 
DQLKPGDYVA VPKILPGKDD KEKFLQYVHE KLKGKVHIKL PSSDEEWETF FYFAGTIFGR 

       190        200        210        220        230        240 
ENSVNPEGLT HEVKALLELF KVLFEYPREV LRVLFMAPVR YVANFLRGFF DINGYVNGEE 

       250        260        270        280        290        300 
LRVEVRGAPH EVLEELSLIL LRLGIVSKIY PTSLAISGRR NLELFRRYIG FSEKQKAKEL 

       310        320        330        340        350        360 
EGIIRRSENS ESYPIFEELR RIRLLFGFTR AELSSTIPLY SKYESKEAPS YEILMKILNT 

       370        380        390        400        410        420 
IEKGSKDLNK KITILEGRVR DHEYIEEFKR EGLIKDGKLT ELGKELLEVW RNREFDSRDV 

       430        440        450        460        470        480 
NYLRNIIENF VFLPVEKIEE FEYDGYVYDV TTETHNFIAN GILVHNTTLL DKIRKTNVAA 

       490        500        510        520        530        540 
KEAGGITQHI GATEVPIDVV KKIAGPLIKL WKAEIRLPGL LFIDTPGHEA FTSLRARGGS 

       550        560        570        580        590        600 
LADLAVLVID VNEGFQPQTI ESIEILRRYR TPFVVAANKI DRIRGWVIEE DEPFLMNIKR 

       610        620        630        640        650        660 
QDQRAIQELE TKLWELIGKF YEFGFQANRF DRVQNFTREL AIVPISAKYG IGIAELLVLI 

       670        680        690        700        710        720 
AGLSQKYLEE KLKIEVEGPA RGTILEVREE PGLGHTIDVI IYDGTLHKDD TIVVGGKDKA 

       730        740        750        760        770        780 
IVTKVRALLK PKPLDEIRDP RFRFDYVDEV TAAAGVKIAA PGLEEALAGS PVIAAPTPED 

       790        800        810        820        830        840 
VERAKEEIMR QIESVVISTD KVGVIVKADT LGSLEALSKE LQEKEIPIRK ADVGNISKTD 

       850        860        870        880        890        900 
VMEALSVKEE NPKYGVILGF NVKVNEDAKE VAKAKEVPIF VGNIIYKLIE DYEAWIKEEE 

       910        920        930        940        950        960 
EKRKRELLAK VTFPGVIKLY PDERYVFRRS NPAIVGIEVL EGRIKPGVTL IKQNGQKVGT 

       970        980        990       1000       1010       1020 
IRSIKSRDEF LQEARKGQAV AIAIEGAIVG RHIHPGETLY VDLSRDDAII LLKHLRDVLE 

      1030       1040 
DTDIKALKMI AQVKAKEDPF WRAV 

« Hide

References

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BA000001 Genomic DNA. Translation: BAA30194.1.
PIRH71049.
RefSeqNP_143000.1. NC_000961.1.

3D structure databases

ProteinModelPortalO58822.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING70601.PH1095.

Protein family/group databases

MEROPSN10.004.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAA30194; BAA30194; BAA30194.
GeneID1443414.
KEGGpho:PH1095.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0532.
HOGENOMHOG000105770.
KOK03243.
OMADHGKCLL.
ProtClustDBPRK14845.

Enzyme and pathway databases

BioCycPHOR70601:GJWR-1085-MONOMER.

Family and domain databases

Gene3D3.10.28.10. 1 hit.
3.40.50.10050. 1 hit.
3.40.50.300. 1 hit.
HAMAPMF_00100_A. IF_2_A.
InterProIPR000795. EF_GTP-bd_dom.
IPR003586. Hint_dom_C.
IPR003587. Hint_dom_N.
IPR027434. Homing_endonucl.
IPR006142. INTEIN.
IPR004042. Intein_endonuc.
IPR006141. Intein_splice_site.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR004544. TIF_aIF-2_arc.
IPR015760. TIF_IF2.
IPR023115. TIF_IF2_dom3.
IPR009000. Transl_B-barrel.
IPR004161. Transl_elong_EFTu/EF1A_2.
[Graphical view]
PANTHERPTHR23115:SF41. PTHR23115:SF41. 1 hit.
PfamPF00009. GTP_EFTU. 1 hit.
PF03144. GTP_EFTU_D2. 1 hit.
PF11987. IF-2. 1 hit.
PF14528. LAGLIDADG_3. 1 hit.
[Graphical view]
PRINTSPR00379. INTEIN.
SMARTSM00305. HintC. 1 hit.
SM00306. HintN. 1 hit.
[Graphical view]
SUPFAMSSF50447. SSF50447. 1 hit.
SSF52156. SSF52156. 1 hit.
SSF52540. SSF52540. 1 hit.
SSF55608. SSF55608. 1 hit.
TIGRFAMsTIGR00491. aIF-2. 1 hit.
TIGR01443. intein_Cterm. 1 hit.
TIGR01445. intein_Nterm. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEPS50818. INTEIN_C_TER. 1 hit.
PS50819. INTEIN_ENDONUCLEASE. 1 hit.
PS50817. INTEIN_N_TER. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameIF2P_PYRHO
AccessionPrimary (citable) accession number: O58822
Entry history
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: August 1, 1998
Last modified: April 16, 2014
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Intein-containing proteins

List of intein-containing protein entries

Translation initiation factors

List of translation initiation factor entries