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O58822

- IF2P_PYRHO

UniProt

O58822 - IF2P_PYRHO

Protein

Probable translation initiation factor IF-2

Gene

infB

Organism
Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 105 (01 Oct 2014)
      Sequence version 1 (01 Aug 1998)
      Previous versions | rss
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    Functioni

    Function in general translation initiation by promoting the binding of the formylmethionine-tRNA to ribosomes. Seems to function along with eIF-2 By similarity.By similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi524 – 5285GTPBy similarity
    Nucleotide bindingi578 – 5814GTPBy similarity

    GO - Molecular functioni

    1. endonuclease activity Source: UniProtKB-KW
    2. GTPase activity Source: InterPro
    3. GTP binding Source: UniProtKB-KW
    4. translation initiation factor activity Source: UniProtKB-KW

    GO - Biological processi

    1. intein-mediated protein splicing Source: InterPro
    2. intron homing Source: UniProtKB-KW

    Keywords - Molecular functioni

    Endonuclease, Hydrolase, Initiation factor, Nuclease

    Keywords - Biological processi

    Intron homing, Protein biosynthesis

    Keywords - Ligandi

    GTP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciPHOR70601:GJWR-1085-MONOMER.

    Protein family/group databases

    MEROPSiN10.004.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Probable translation initiation factor IF-2
    Cleaved into the following chain:
    Alternative name(s):
    Pho IF2 intein
    Gene namesi
    Name:infB
    Ordered Locus Names:PH1095
    OrganismiPyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
    Taxonomic identifieri70601 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
    ProteomesiUP000000752: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    1. intracellular Source: InterPro

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 2222Probable translation initiation factor IF-2, 1st partSequence AnalysisPRO_0000014493Add
    BLAST
    Chaini23 – 466444Pho infB inteinSequence AnalysisPRO_0000014494Add
    BLAST
    Chaini467 – 1044578Probable translation initiation factor IF-2, 2nd partSequence AnalysisPRO_0000014495Add
    BLAST

    Post-translational modificationi

    This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation.Curated

    Keywords - PTMi

    Autocatalytic cleavage, Protein splicing

    Interactioni

    Protein-protein interaction databases

    STRINGi70601.PH1095.

    Structurei

    3D structure databases

    ProteinModelPortaliO58822.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini173 – 26593DOD-type homing endonucleaseAdd
    BLAST
    Domaini451 – 668218tr-type GAdd
    BLAST

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0532.
    HOGENOMiHOG000105770.
    KOiK03243.
    OMAiDHGKCLL.

    Family and domain databases

    Gene3Di2.170.16.10. 2 hits.
    3.10.28.10. 1 hit.
    3.40.50.10050. 1 hit.
    3.40.50.300. 1 hit.
    HAMAPiMF_00100_A. IF_2_A.
    InterProiIPR000795. EF_GTP-bd_dom.
    IPR029459. EFTU-type.
    IPR028992. Hedgehog/Intein_dom.
    IPR003586. Hint_dom_C.
    IPR003587. Hint_dom_N.
    IPR027434. Homing_endonucl.
    IPR006142. INTEIN.
    IPR004042. Intein_endonuc.
    IPR006141. Intein_splice_site.
    IPR027417. P-loop_NTPase.
    IPR005225. Small_GTP-bd_dom.
    IPR004544. TIF_aIF-2_arc.
    IPR023115. TIF_IF2_dom3.
    IPR009000. Transl_B-barrel.
    IPR004161. Transl_elong_EFTu/EF1A_2.
    [Graphical view]
    PfamiPF00009. GTP_EFTU. 1 hit.
    PF03144. GTP_EFTU_D2. 1 hit.
    PF14578. GTP_EFTU_D4. 1 hit.
    PF11987. IF-2. 1 hit.
    PF14528. LAGLIDADG_3. 1 hit.
    [Graphical view]
    PRINTSiPR00379. INTEIN.
    SMARTiSM00305. HintC. 1 hit.
    SM00306. HintN. 1 hit.
    [Graphical view]
    SUPFAMiSSF50447. SSF50447. 1 hit.
    SSF51294. SSF51294. 2 hits.
    SSF52156. SSF52156. 1 hit.
    SSF52540. SSF52540. 1 hit.
    SSF55608. SSF55608. 1 hit.
    TIGRFAMsiTIGR00491. aIF-2. 1 hit.
    TIGR01443. intein_Cterm. 1 hit.
    TIGR01445. intein_Nterm. 1 hit.
    TIGR00231. small_GTP. 1 hit.
    PROSITEiPS51722. G_TR_2. 1 hit.
    PS50818. INTEIN_C_TER. 1 hit.
    PS50819. INTEIN_ENDONUCLEASE. 1 hit.
    PS50817. INTEIN_N_TER. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    O58822-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSYVKRIRQP IIAVLGHVDH GKCLLPEERV ILPDYGPITL EELFNMTKET     50
    VFKDEEKEVR KLGIRMPVAG VDGRVRLLEG PYVWKVRYKG KMLRVKLKDW 100
    HSVAVTPEHP FLTTRGWVRA DQLKPGDYVA VPKILPGKDD KEKFLQYVHE 150
    KLKGKVHIKL PSSDEEWETF FYFAGTIFGR ENSVNPEGLT HEVKALLELF 200
    KVLFEYPREV LRVLFMAPVR YVANFLRGFF DINGYVNGEE LRVEVRGAPH 250
    EVLEELSLIL LRLGIVSKIY PTSLAISGRR NLELFRRYIG FSEKQKAKEL 300
    EGIIRRSENS ESYPIFEELR RIRLLFGFTR AELSSTIPLY SKYESKEAPS 350
    YEILMKILNT IEKGSKDLNK KITILEGRVR DHEYIEEFKR EGLIKDGKLT 400
    ELGKELLEVW RNREFDSRDV NYLRNIIENF VFLPVEKIEE FEYDGYVYDV 450
    TTETHNFIAN GILVHNTTLL DKIRKTNVAA KEAGGITQHI GATEVPIDVV 500
    KKIAGPLIKL WKAEIRLPGL LFIDTPGHEA FTSLRARGGS LADLAVLVID 550
    VNEGFQPQTI ESIEILRRYR TPFVVAANKI DRIRGWVIEE DEPFLMNIKR 600
    QDQRAIQELE TKLWELIGKF YEFGFQANRF DRVQNFTREL AIVPISAKYG 650
    IGIAELLVLI AGLSQKYLEE KLKIEVEGPA RGTILEVREE PGLGHTIDVI 700
    IYDGTLHKDD TIVVGGKDKA IVTKVRALLK PKPLDEIRDP RFRFDYVDEV 750
    TAAAGVKIAA PGLEEALAGS PVIAAPTPED VERAKEEIMR QIESVVISTD 800
    KVGVIVKADT LGSLEALSKE LQEKEIPIRK ADVGNISKTD VMEALSVKEE 850
    NPKYGVILGF NVKVNEDAKE VAKAKEVPIF VGNIIYKLIE DYEAWIKEEE 900
    EKRKRELLAK VTFPGVIKLY PDERYVFRRS NPAIVGIEVL EGRIKPGVTL 950
    IKQNGQKVGT IRSIKSRDEF LQEARKGQAV AIAIEGAIVG RHIHPGETLY 1000
    VDLSRDDAII LLKHLRDVLE DTDIKALKMI AQVKAKEDPF WRAV 1044
    Length:1,044
    Mass (Da):118,951
    Last modified:August 1, 1998 - v1
    Checksum:i907C7A84D0ACB3F4
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000001 Genomic DNA. Translation: BAA30194.1.
    PIRiH71049.
    RefSeqiNP_143000.1. NC_000961.1.
    WP_010885179.1. NC_000961.1.

    Genome annotation databases

    EnsemblBacteriaiBAA30194; BAA30194; BAA30194.
    GeneIDi1443414.
    KEGGipho:PH1095.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000001 Genomic DNA. Translation: BAA30194.1 .
    PIRi H71049.
    RefSeqi NP_143000.1. NC_000961.1.
    WP_010885179.1. NC_000961.1.

    3D structure databases

    ProteinModelPortali O58822.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 70601.PH1095.

    Protein family/group databases

    MEROPSi N10.004.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAA30194 ; BAA30194 ; BAA30194 .
    GeneIDi 1443414.
    KEGGi pho:PH1095.

    Phylogenomic databases

    eggNOGi COG0532.
    HOGENOMi HOG000105770.
    KOi K03243.
    OMAi DHGKCLL.

    Enzyme and pathway databases

    BioCyci PHOR70601:GJWR-1085-MONOMER.

    Family and domain databases

    Gene3Di 2.170.16.10. 2 hits.
    3.10.28.10. 1 hit.
    3.40.50.10050. 1 hit.
    3.40.50.300. 1 hit.
    HAMAPi MF_00100_A. IF_2_A.
    InterProi IPR000795. EF_GTP-bd_dom.
    IPR029459. EFTU-type.
    IPR028992. Hedgehog/Intein_dom.
    IPR003586. Hint_dom_C.
    IPR003587. Hint_dom_N.
    IPR027434. Homing_endonucl.
    IPR006142. INTEIN.
    IPR004042. Intein_endonuc.
    IPR006141. Intein_splice_site.
    IPR027417. P-loop_NTPase.
    IPR005225. Small_GTP-bd_dom.
    IPR004544. TIF_aIF-2_arc.
    IPR023115. TIF_IF2_dom3.
    IPR009000. Transl_B-barrel.
    IPR004161. Transl_elong_EFTu/EF1A_2.
    [Graphical view ]
    Pfami PF00009. GTP_EFTU. 1 hit.
    PF03144. GTP_EFTU_D2. 1 hit.
    PF14578. GTP_EFTU_D4. 1 hit.
    PF11987. IF-2. 1 hit.
    PF14528. LAGLIDADG_3. 1 hit.
    [Graphical view ]
    PRINTSi PR00379. INTEIN.
    SMARTi SM00305. HintC. 1 hit.
    SM00306. HintN. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50447. SSF50447. 1 hit.
    SSF51294. SSF51294. 2 hits.
    SSF52156. SSF52156. 1 hit.
    SSF52540. SSF52540. 1 hit.
    SSF55608. SSF55608. 1 hit.
    TIGRFAMsi TIGR00491. aIF-2. 1 hit.
    TIGR01443. intein_Cterm. 1 hit.
    TIGR01445. intein_Nterm. 1 hit.
    TIGR00231. small_GTP. 1 hit.
    PROSITEi PS51722. G_TR_2. 1 hit.
    PS50818. INTEIN_C_TER. 1 hit.
    PS50819. INTEIN_ENDONUCLEASE. 1 hit.
    PS50817. INTEIN_N_TER. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3.

    Entry informationi

    Entry nameiIF2P_PYRHO
    AccessioniPrimary (citable) accession number: O58822
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 15, 1998
    Last sequence update: August 1, 1998
    Last modified: October 1, 2014
    This is version 105 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    The intein interrupts the GTP-binding site.

    Keywords - Technical termi

    Complete proteome

    Documents

    1. Intein-containing proteins
      List of intein-containing protein entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3