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O58822

- IF2P_PYRHO

UniProt

O58822 - IF2P_PYRHO

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Protein
Probable translation initiation factor IF-2
Gene
infB, PH1095
Organism
Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Function in general translation initiation by promoting the binding of the formylmethionine-tRNA to ribosomes. Seems to function along with eIF-2 By similarity.UniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi524 – 5285GTP By similarity
Nucleotide bindingi578 – 5814GTP By similarity

GO - Molecular functioni

  1. GTP binding Source: UniProtKB-KW
  2. GTPase activity Source: InterPro
  3. endonuclease activity Source: UniProtKB-KW
  4. translation initiation factor activity Source: UniProtKB-KW

GO - Biological processi

  1. intein-mediated protein splicing Source: InterPro
  2. intron homing Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Initiation factor, Nuclease

Keywords - Biological processi

Intron homing, Protein biosynthesis

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciPHOR70601:GJWR-1085-MONOMER.

Protein family/group databases

MEROPSiN10.004.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable translation initiation factor IF-2
Cleaved into the following chain:
Alternative name(s):
Pho IF2 intein
Gene namesi
Name:infB
Ordered Locus Names:PH1095
OrganismiPyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Taxonomic identifieri70601 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
ProteomesiUP000000752: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. intracellular Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 2222Probable translation initiation factor IF-2, 1st part Reviewed prediction
PRO_0000014493Add
BLAST
Chaini23 – 466444Pho infB intein Reviewed prediction
PRO_0000014494Add
BLAST
Chaini467 – 1044578Probable translation initiation factor IF-2, 2nd part Reviewed prediction
PRO_0000014495Add
BLAST

Post-translational modificationi

This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation Reviewed prediction.UniRule annotation

Keywords - PTMi

Autocatalytic cleavage, Protein splicing

Interactioni

Protein-protein interaction databases

STRINGi70601.PH1095.

Structurei

3D structure databases

ProteinModelPortaliO58822.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini173 – 26593DOD-type homing endonuclease
Add
BLAST
Domaini451 – 668218tr-type G
Add
BLAST

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0532.
HOGENOMiHOG000105770.
KOiK03243.
OMAiDHGKCLL.

Family and domain databases

Gene3Di2.170.16.10. 2 hits.
3.10.28.10. 1 hit.
3.40.50.10050. 1 hit.
3.40.50.300. 1 hit.
HAMAPiMF_00100_A. IF_2_A.
InterProiIPR000795. EF_GTP-bd_dom.
IPR029459. EFTU-type.
IPR028992. Hedgehog/Intein_dom.
IPR003586. Hint_dom_C.
IPR003587. Hint_dom_N.
IPR027434. Homing_endonucl.
IPR006142. INTEIN.
IPR004042. Intein_endonuc.
IPR006141. Intein_splice_site.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR004544. TIF_aIF-2_arc.
IPR023115. TIF_IF2_dom3.
IPR009000. Transl_B-barrel.
IPR004161. Transl_elong_EFTu/EF1A_2.
[Graphical view]
PfamiPF00009. GTP_EFTU. 1 hit.
PF03144. GTP_EFTU_D2. 1 hit.
PF14578. GTP_EFTU_D4. 1 hit.
PF11987. IF-2. 1 hit.
PF14528. LAGLIDADG_3. 1 hit.
[Graphical view]
PRINTSiPR00379. INTEIN.
SMARTiSM00305. HintC. 1 hit.
SM00306. HintN. 1 hit.
[Graphical view]
SUPFAMiSSF50447. SSF50447. 1 hit.
SSF51294. SSF51294. 2 hits.
SSF52156. SSF52156. 1 hit.
SSF52540. SSF52540. 1 hit.
SSF55608. SSF55608. 1 hit.
TIGRFAMsiTIGR00491. aIF-2. 1 hit.
TIGR01443. intein_Cterm. 1 hit.
TIGR01445. intein_Nterm. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEiPS51722. G_TR_2. 1 hit.
PS50818. INTEIN_C_TER. 1 hit.
PS50819. INTEIN_ENDONUCLEASE. 1 hit.
PS50817. INTEIN_N_TER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O58822-1 [UniParc]FASTAAdd to Basket

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MSYVKRIRQP IIAVLGHVDH GKCLLPEERV ILPDYGPITL EELFNMTKET     50
VFKDEEKEVR KLGIRMPVAG VDGRVRLLEG PYVWKVRYKG KMLRVKLKDW 100
HSVAVTPEHP FLTTRGWVRA DQLKPGDYVA VPKILPGKDD KEKFLQYVHE 150
KLKGKVHIKL PSSDEEWETF FYFAGTIFGR ENSVNPEGLT HEVKALLELF 200
KVLFEYPREV LRVLFMAPVR YVANFLRGFF DINGYVNGEE LRVEVRGAPH 250
EVLEELSLIL LRLGIVSKIY PTSLAISGRR NLELFRRYIG FSEKQKAKEL 300
EGIIRRSENS ESYPIFEELR RIRLLFGFTR AELSSTIPLY SKYESKEAPS 350
YEILMKILNT IEKGSKDLNK KITILEGRVR DHEYIEEFKR EGLIKDGKLT 400
ELGKELLEVW RNREFDSRDV NYLRNIIENF VFLPVEKIEE FEYDGYVYDV 450
TTETHNFIAN GILVHNTTLL DKIRKTNVAA KEAGGITQHI GATEVPIDVV 500
KKIAGPLIKL WKAEIRLPGL LFIDTPGHEA FTSLRARGGS LADLAVLVID 550
VNEGFQPQTI ESIEILRRYR TPFVVAANKI DRIRGWVIEE DEPFLMNIKR 600
QDQRAIQELE TKLWELIGKF YEFGFQANRF DRVQNFTREL AIVPISAKYG 650
IGIAELLVLI AGLSQKYLEE KLKIEVEGPA RGTILEVREE PGLGHTIDVI 700
IYDGTLHKDD TIVVGGKDKA IVTKVRALLK PKPLDEIRDP RFRFDYVDEV 750
TAAAGVKIAA PGLEEALAGS PVIAAPTPED VERAKEEIMR QIESVVISTD 800
KVGVIVKADT LGSLEALSKE LQEKEIPIRK ADVGNISKTD VMEALSVKEE 850
NPKYGVILGF NVKVNEDAKE VAKAKEVPIF VGNIIYKLIE DYEAWIKEEE 900
EKRKRELLAK VTFPGVIKLY PDERYVFRRS NPAIVGIEVL EGRIKPGVTL 950
IKQNGQKVGT IRSIKSRDEF LQEARKGQAV AIAIEGAIVG RHIHPGETLY 1000
VDLSRDDAII LLKHLRDVLE DTDIKALKMI AQVKAKEDPF WRAV 1044
Length:1,044
Mass (Da):118,951
Last modified:August 1, 1998 - v1
Checksum:i907C7A84D0ACB3F4
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000001 Genomic DNA. Translation: BAA30194.1.
PIRiH71049.
RefSeqiNP_143000.1. NC_000961.1.
WP_010885179.1. NC_000961.1.

Genome annotation databases

EnsemblBacteriaiBAA30194; BAA30194; BAA30194.
GeneIDi1443414.
KEGGipho:PH1095.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000001 Genomic DNA. Translation: BAA30194.1 .
PIRi H71049.
RefSeqi NP_143000.1. NC_000961.1.
WP_010885179.1. NC_000961.1.

3D structure databases

ProteinModelPortali O58822.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 70601.PH1095.

Protein family/group databases

MEROPSi N10.004.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAA30194 ; BAA30194 ; BAA30194 .
GeneIDi 1443414.
KEGGi pho:PH1095.

Phylogenomic databases

eggNOGi COG0532.
HOGENOMi HOG000105770.
KOi K03243.
OMAi DHGKCLL.

Enzyme and pathway databases

BioCyci PHOR70601:GJWR-1085-MONOMER.

Family and domain databases

Gene3Di 2.170.16.10. 2 hits.
3.10.28.10. 1 hit.
3.40.50.10050. 1 hit.
3.40.50.300. 1 hit.
HAMAPi MF_00100_A. IF_2_A.
InterProi IPR000795. EF_GTP-bd_dom.
IPR029459. EFTU-type.
IPR028992. Hedgehog/Intein_dom.
IPR003586. Hint_dom_C.
IPR003587. Hint_dom_N.
IPR027434. Homing_endonucl.
IPR006142. INTEIN.
IPR004042. Intein_endonuc.
IPR006141. Intein_splice_site.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR004544. TIF_aIF-2_arc.
IPR023115. TIF_IF2_dom3.
IPR009000. Transl_B-barrel.
IPR004161. Transl_elong_EFTu/EF1A_2.
[Graphical view ]
Pfami PF00009. GTP_EFTU. 1 hit.
PF03144. GTP_EFTU_D2. 1 hit.
PF14578. GTP_EFTU_D4. 1 hit.
PF11987. IF-2. 1 hit.
PF14528. LAGLIDADG_3. 1 hit.
[Graphical view ]
PRINTSi PR00379. INTEIN.
SMARTi SM00305. HintC. 1 hit.
SM00306. HintN. 1 hit.
[Graphical view ]
SUPFAMi SSF50447. SSF50447. 1 hit.
SSF51294. SSF51294. 2 hits.
SSF52156. SSF52156. 1 hit.
SSF52540. SSF52540. 1 hit.
SSF55608. SSF55608. 1 hit.
TIGRFAMsi TIGR00491. aIF-2. 1 hit.
TIGR01443. intein_Cterm. 1 hit.
TIGR01445. intein_Nterm. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEi PS51722. G_TR_2. 1 hit.
PS50818. INTEIN_C_TER. 1 hit.
PS50819. INTEIN_ENDONUCLEASE. 1 hit.
PS50817. INTEIN_N_TER. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3.

Entry informationi

Entry nameiIF2P_PYRHO
AccessioniPrimary (citable) accession number: O58822
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: August 1, 1998
Last modified: September 3, 2014
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

The intein interrupts the GTP-binding site.

Keywords - Technical termi

Complete proteome

Documents

  1. Intein-containing proteins
    List of intein-containing protein entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi