O58822 (IF2P_PYRHO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable translation initiation factor IF-2

Cleaved into the following chain:

1. Pho infB intein
   Alternative name(s):
   Pho IF2 intein

Gene names

Name:	infB
Ordered Locus Names:	PH1095

Organism

Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3) [Complete proteome] [HAMAP]

Taxonomic identifier

70601 [NCBI]

Taxonomic lineage

cellular organisms › Archaea › Euryarchaeota › Thermococci › Thermococcales › Thermococcaceae › Pyrococcus › Pyrococcus horikoshii

Protein attributes

Sequence length	1044 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Function in general translation initiation by promoting the binding of the formylmethionine-tRNA to ribosomes. Seems to function along with eIF-2 By similarity. HAMAP-Rule MF_00100_A
Post-translational modification	This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation Potential. HAMAP-Rule MF_00100_A
Miscellaneous	The intein interrupts the GTP-binding site. HAMAP-Rule MF_00100_A
Sequence similarities	Belongs to the IF-2 family. Contains 1 DOD-type homing endonuclease domain.

Ontologies

Keywords
Biological process	Intron homing Protein biosynthesis
Ligand	GTP-binding Nucleotide-binding
Molecular function	Endonuclease Hydrolase Initiation factor Nuclease
PTM	Autocatalytic cleavage Protein splicing
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	GTP catabolic process Inferred from electronic annotation. Source: GOC intein-mediated protein splicing Inferred from electronic annotation. Source: InterPro intron homing Inferred from electronic annotation. Source: UniProtKB-KW nucleic acid phosphodiester bond hydrolysis Inferred from electronic annotation. Source: GOC
Cellular_component	intracellular Inferred from electronic annotation. Source: InterPro
Molecular_function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW GTPase activity Inferred from electronic annotation. Source: HAMAP endonuclease activity Inferred from electronic annotation. Source: UniProtKB-KW translation initiation factor activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 22	22	Probable translation initiation factor IF-2, 1st part Potential		PRO_0000014493
	Chain	23 – 466	444	Pho infB intein Potential		PRO_0000014494
	Chain	467 – 1044	578	Probable translation initiation factor IF-2, 2nd part Potential		PRO_0000014495
Regions
	Domain	173 – 265	93	DOD-type homing endonuclease
	Nucleotide binding	524 – 528	5	GTP By similarity
	Nucleotide binding	578 – 581	4	GTP By similarity

Sequences

	Sequence		Length	Mass (Da)
	O58822 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: 907C7A84D0ACB3F4	FASTA	1,044	118,951
	10 20 30 40 50 60 MSYVKRIRQP IIAVLGHVDH GKCLLPEERV ILPDYGPITL EELFNMTKET VFKDEEKEVR 70 80 90 100 110 120 KLGIRMPVAG VDGRVRLLEG PYVWKVRYKG KMLRVKLKDW HSVAVTPEHP FLTTRGWVRA 130 140 150 160 170 180 DQLKPGDYVA VPKILPGKDD KEKFLQYVHE KLKGKVHIKL PSSDEEWETF FYFAGTIFGR 190 200 210 220 230 240 ENSVNPEGLT HEVKALLELF KVLFEYPREV LRVLFMAPVR YVANFLRGFF DINGYVNGEE 250 260 270 280 290 300 LRVEVRGAPH EVLEELSLIL LRLGIVSKIY PTSLAISGRR NLELFRRYIG FSEKQKAKEL 310 320 330 340 350 360 EGIIRRSENS ESYPIFEELR RIRLLFGFTR AELSSTIPLY SKYESKEAPS YEILMKILNT 370 380 390 400 410 420 IEKGSKDLNK KITILEGRVR DHEYIEEFKR EGLIKDGKLT ELGKELLEVW RNREFDSRDV 430 440 450 460 470 480 NYLRNIIENF VFLPVEKIEE FEYDGYVYDV TTETHNFIAN GILVHNTTLL DKIRKTNVAA 490 500 510 520 530 540 KEAGGITQHI GATEVPIDVV KKIAGPLIKL WKAEIRLPGL LFIDTPGHEA FTSLRARGGS 550 560 570 580 590 600 LADLAVLVID VNEGFQPQTI ESIEILRRYR TPFVVAANKI DRIRGWVIEE DEPFLMNIKR 610 620 630 640 650 660 QDQRAIQELE TKLWELIGKF YEFGFQANRF DRVQNFTREL AIVPISAKYG IGIAELLVLI 670 680 690 700 710 720 AGLSQKYLEE KLKIEVEGPA RGTILEVREE PGLGHTIDVI IYDGTLHKDD TIVVGGKDKA 730 740 750 760 770 780 IVTKVRALLK PKPLDEIRDP RFRFDYVDEV TAAAGVKIAA PGLEEALAGS PVIAAPTPED 790 800 810 820 830 840 VERAKEEIMR QIESVVISTD KVGVIVKADT LGSLEALSKE LQEKEIPIRK ADVGNISKTD 850 860 870 880 890 900 VMEALSVKEE NPKYGVILGF NVKVNEDAKE VAKAKEVPIF VGNIIYKLIE DYEAWIKEEE 910 920 930 940 950 960 EKRKRELLAK VTFPGVIKLY PDERYVFRRS NPAIVGIEVL EGRIKPGVTL IKQNGQKVGT 970 980 990 1000 1010 1020 IRSIKSRDEF LQEARKGQAV AIAIEGAIVG RHIHPGETLY VDLSRDDAII LLKHLRDVLE 1030 1040 DTDIKALKMI AQVKAKEDPF WRAV

References

[1]

"Complete sequence and gene organization of the genome of a hyper-thermophilic archaebacterium, Pyrococcus horikoshii OT3." Kawarabayasi Y., Sawada M., Horikawa H., Haikawa Y., Hino Y., Yamamoto S., Sekine M., Baba S., Kosugi H., Hosoyama A., Nagai Y., Sakai M., Ogura K., Otsuka R., Nakazawa H., Takamiya M., Ohfuku Y., Funahashi T. , Tanaka T., Kudoh Y., Yamazaki J., Kushida N., Oguchi A., Aoki K., Yoshizawa T., Nakamura Y., Robb F.T., Horikoshi K., Masuchi Y., Shizuya H., Kikuchi H. DNA Res. 5:55-76(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000001 Genomic DNA. Translation: BAA30194.1.
PIR	H71049.
RefSeq	NP_143000.1. NC_000961.1.
3D structure databases
ProteinModelPortal	O58822.
ModBase	Search...
Protein-protein interaction databases
STRING	70601.PH1095.
Protein family/group databases
MEROPS	N10.004.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	BAA30194; BAA30194; BAA30194.
GeneID	1443414.
KEGG	pho:PH1095.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0532.
HOGENOM	HOG000105770.
KO	K03243.
OMA	EVWRNRE.
ProtClustDB	PRK14845.
Enzyme and pathway databases
BioCyc	PHOR70601:GJWR-1085-MONOMER.
Family and domain databases
Gene3D	3.10.28.10. 1 hit. 3.40.50.10050. 1 hit.
HAMAP	MF_00100_A. IF-2_A.
InterPro	IPR000795. EF_GTP-bd_dom. IPR003586. Hint_dom_C. IPR003587. Hint_dom_N. IPR027434. Homing_endonucl. IPR006142. INTEIN. IPR004042. Intein_endonuc. IPR006141. Intein_splice_site. IPR027417. P-loop_NTPase. IPR005225. Small_GTP-bd_dom. IPR004544. TIF_aIF-2_arc. IPR015760. TIF_IF2. IPR023115. TIF_IF2_dom3. IPR004161. Transl_elong_EFTu/EF1A_2. IPR009000. Transl_elong_init/rib_B-barrel. [Graphical view]
PANTHER	PTHR23115:SF41. PTHR23115:SF41. 1 hit.
Pfam	PF00009. GTP_EFTU. 1 hit. PF03144. GTP_EFTU_D2. 1 hit. PF11987. IF-2. 1 hit. [Graphical view]
PRINTS	PR00379. INTEIN.
SMART	SM00305. HintC. 1 hit. SM00306. HintN. 1 hit. [Graphical view]
SUPFAM	SSF52540. SSF52540. 1 hit. SSF55608. SSF55608. 1 hit. SSF52156. TIF_IF2_dom3. 1 hit. SSF50447. Translat_factor. 1 hit.
TIGRFAMs	TIGR00491. aIF-2. 1 hit. TIGR01443. intein_Cterm. 1 hit. TIGR01445. intein_Nterm. 1 hit. TIGR00231. small_GTP. 1 hit.
PROSITE	PS01176. IF2. False negative. PS50818. INTEIN_C_TER. 1 hit. PS50819. INTEIN_ENDONUCLEASE. 1 hit. PS50817. INTEIN_N_TER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

IF2P_PYRHO

Accession

Primary (citable) accession number: O58822

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	August 1, 1998
Last modified:	May 29, 2013
This is version 96 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program