O58814 (KHSE_PYRHO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Homoserine kinase
Short name=HK
Short name=HSK
EC=2.7.1.39

Gene names

Name:	thrB
Ordered Locus Names:	PH1087

Organism

Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3) [Complete proteome] [HAMAP]

Taxonomic identifier

70601 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Thermococci › Thermococcales › Thermococcaceae › Pyrococcus

Protein attributes

Sequence length	293 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the ATP-dependent phosphorylation of L-homoserine to L-homoserine phosphate By similarity. HAMAP MF_00384
Catalytic activity	ATP + L-homoserine = ADP + O-phospho-L-homoserine. HAMAP MF_00384
Pathway	Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 4/5. HAMAP MF_00384
Subcellular location	Cytoplasm Potential HAMAP MF_00384.
Sequence similarities	Belongs to the GHMP kinase family. Homoserine kinase subfamily.
Sequence caution	The sequence BAA30186.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Threonine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	threonine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW homoserine kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 293	293	Homoserine kinase HAMAP MF_00384		PRO_0000156647
Regions
	Nucleotide binding	83 – 93	11	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	O58814 [UniParc]. Last modified June 1, 2001. Version 2. Checksum: 33148C4B26232F63	FASTA	293	31,485
	10 20 30 40 50 60 MRIKVRAPAT IANFGPGFDV FGMAIDKPFD EVVVEEFNEF EIISSGYPVP NGEDNIALFS 70 80 90 100 110 120 AKTLFKMLNI EGGLRIKLKK GIRPKSGLGS SGASAVAGAL GAAKLLGVSN DELILKAAMK 130 140 150 160 170 180 GEEKASGEPH PDNVVPSYYG GFTVIESKSP LRVHFVDAKL RGVVVLPEVE IPTAKARKIL 190 200 210 220 230 240 PSMVPLKDAV KNIAMASSLI LALKEGDLET IGRLLDDNLA LPYRKKLMPW FDEIRRVALE 250 260 270 280 290 TGAYGITVSG SGPALFAIGE NLKDIGKTIV EKFEELGIKA EYWVTKTGRG AKT

References

[1]

"Complete sequence and gene organization of the genome of a hyper-thermophilic archaebacterium, Pyrococcus horikoshii OT3." Kawarabayasi Y., Sawada M., Horikawa H., Haikawa Y., Hino Y., Yamamoto S., Sekine M., Baba S., Kosugi H., Hosoyama A., Nagai Y., Sakai M., Ogura K., Otsuka R., Nakazawa H., Takamiya M., Ohfuku Y., Funahashi T. , Tanaka T., Kudoh Y., Yamazaki J., Kushida N., Oguchi A., Aoki K., Yoshizawa T., Nakamura Y., Robb F.T., Horikoshi K., Masuchi Y., Shizuya H., Kikuchi H. DNA Res. 5:55-76(1998) [PubMed: 9679194] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000001 Genomic DNA. Translation: BAA30186.1. Different initiation.
PIR	D71103.
RefSeq	NP_142994.2. NC_000961.1.
3D structure databases
ProteinModelPortal	O58814.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBPYRT00000001635; EBPYRP00000001635; EBPYRG00000001635.
GeneID	1443408.
GenomeReviews	Gene locus PH1087 in contig BA000001_GR.
KEGG	pho:PH1087.
NMPDR	fig|70601.1.peg.1061.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000022725.
HOGENOM	HBG646290.
OMA	NNALMST.
PhylomeDB	O58814.
ProtClustDB	PRK01212.
Enzyme and pathway databases
BioCyc	PHOR70601:PH1087-MONOMER.
Family and domain databases
HAMAP	MF_00384. Homoser_kinase. [Tree]
InterPro	IPR006204. GHMP_kinase. IPR013750. GHMP_kinase_C. IPR000870. Homoserine_kinase. IPR020568. Ribosomal_S5_D2-typ_fold. IPR014721. Ribosomal_S5_D2-typ_fold_subgr. [Graphical view]
Gene3D	G3DSA:3.30.230.10. Ribosomal_S5_D2-type_fold. 1 hit.
KO	K00872.
Pfam	PF08544. GHMP_kinases_C. 1 hit. PF00288. GHMP_kinases_N. 1 hit. [Graphical view]
PIRSF	PIRSF000676. Homoser_kin. 1 hit.
PRINTS	PR00958. HOMSERKINASE.
SUPFAM	SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit.
TIGRFAMs	TIGR00191. ThrB. 1 hit.
PROSITE	PS00627. GHMP_KINASES_ATP. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KHSE_PYRHO

Accession

Primary (citable) accession number: O58814

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 2001
Last sequence update:	June 1, 2001
Last modified:	December 14, 2011
This is version 84 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program