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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei628 – 6281ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciPHOR70601:GJWR-1060-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:PH1065
OrganismiPyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Taxonomic identifieri70601 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
ProteomesiUP000000752 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10661066Isoleucine--tRNA ligasePRO_0000098590Add
BLAST

Proteomic databases

PRIDEiO58792.

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi70601.PH1065.

Structurei

3D structure databases

ProteinModelPortaliO58792.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi49 – 5911"HIGH" regionAdd
BLAST
Motifi625 – 6295"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246403.
KOiK01870.
OMAiIPLPIWR.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02003. Ile_tRNA_synth_type2.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR023586. Ile-tRNA-ligase_type2.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 2 hits.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O58792-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIKEPEFRDY TPGKLEEKIE EFWKENNIYQ KIKELRKNGP KYYFLDGPPY
60 70 80 90 100
VSGAIHLGTA WNKIIKDMII RFRTMQGYNV WRQPGFDMHG LPIEVKVEQA
110 120 130 140 150
LGLKTKKEIE EKIGVENFIK KCKEFALNNL KIMTEQFKML GIWMDWDDPY
160 170 180 190 200
MTIKNEYIES AWFTLKKAWE KGLLEKDKRV LHWCPRCETA LAEHEVRGEY
210 220 230 240 250
KLRKDPSIYV KFPIEGKENE YLLIWTTTPW TLPANLAVSA HPEYDYVKVK
260 270 280 290 300
VEFNGKEEYW ILAKALVDKV LGEIGVKGEV IEEFKGRELE GLRYIHVLMD
310 320 330 340 350
EYPRQKEFRE KYEWAHRIIL ADFVTLEEGT GLVHTAPGHG EEDFEVGKKY
360 370 380 390 400
GLPIYSPVDD QGRYVEGKWK GIYVKEADPQ IIEHLKEKGY LVKAGEIEHK
410 420 430 440 450
YPHCWRCKTP LIFRATDQWF LKVSKVKDRI IKENDEKVTW YPDWVKIRFD
460 470 480 490 500
NGVRDSGDWV ISRQRYWGIP LPIWQSEDGE IYVVGSWREL VELAVAIEVN
510 520 530 540 550
GERIDLPESY EEKLKVIEEK LGPEDLHRPY VDAFIIKVNG KEMRRVKDVV
560 570 580 590 600
DVWFDSGIAS WASLGYPRNK ELFEKLWPAD FIVEGEDQVT KWFYSQQAAS
610 620 630 640 650
VIAFDTVPYR RVAMHGYVLD EKGDKMSKSL GNIIRPEEVV EKAGRDTFRF
660 670 680 690 700
YMLWATNPWE NLKFSWKGVE QVRRMLNILW NVYVLSATYM SLDNFDPRNV
710 720 730 740 750
KVEELAFREE DKWILSRVNS LIREVENGIE TFYLTKATRA LYNFVVEDLS
760 770 780 790 800
RWYVRLIRKR LWVEGDDPDK LAAYYTLWKV FDVLLRLMAP FTPYITEEIY
810 820 830 840 850
QNLMRPFIGI ESVHMLDWPK VDESAVDEDL EKEMEFIRRI VEAGSAARQK
860 870 880 890 900
ARIKLRYPVR KIIIETQDET VKKAVERLNY ILRDQLNAKE VVIGKVEREL
910 920 930 940 950
TVKPNFAKVG PEFKGDSRLV AKWINEHGLE LYEKGEVDVE IEGKKFHLTR
960 970 980 990 1000
EHIIVEEKLP DFLVAEDFEG GRVYVDKTLT RELLAEGLAR EFVRRIQEMR
1010 1020 1030 1040 1050
KRLDLDVNDR IVVTIETTDD NRELLQENLD YIMRETRAIE VRFEEAKGYV
1060
VEWPEVQAKI GIEKVE
Length:1,066
Mass (Da):125,941
Last modified:August 1, 1998 - v1
Checksum:i4C9444C82BB14018
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000001 Genomic DNA. Translation: BAA30164.1.
PIRiF71100.
RefSeqiNP_142976.1. NC_000961.1.
WP_010885154.1. NC_000961.1.

Genome annotation databases

EnsemblBacteriaiBAA30164; BAA30164; BAA30164.
GeneIDi1443389.
KEGGipho:PH1065.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000001 Genomic DNA. Translation: BAA30164.1.
PIRiF71100.
RefSeqiNP_142976.1. NC_000961.1.
WP_010885154.1. NC_000961.1.

3D structure databases

ProteinModelPortaliO58792.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi70601.PH1065.

Proteomic databases

PRIDEiO58792.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAA30164; BAA30164; BAA30164.
GeneIDi1443389.
KEGGipho:PH1065.

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246403.
KOiK01870.
OMAiIPLPIWR.

Enzyme and pathway databases

BioCyciPHOR70601:GJWR-1060-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02003. Ile_tRNA_synth_type2.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR023586. Ile-tRNA-ligase_type2.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 2 hits.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3.

Entry informationi

Entry nameiSYI_PYRHO
AccessioniPrimary (citable) accession number: O58792
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: August 1, 1998
Last modified: February 4, 2015
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.