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O58792

- SYI_PYRHO

UniProt

O58792 - SYI_PYRHO

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 93 (01 Oct 2014)
      Sequence version 1 (01 Aug 1998)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Zinc.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei628 – 6281ATPUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciPHOR70601:GJWR-1060-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:PH1065
    OrganismiPyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
    Taxonomic identifieri70601 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
    ProteomesiUP000000752: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 10661066Isoleucine--tRNA ligasePRO_0000098590Add
    BLAST

    Proteomic databases

    PRIDEiO58792.

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi70601.PH1065.

    Structurei

    3D structure databases

    ProteinModelPortaliO58792.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi49 – 5911"HIGH" regionAdd
    BLAST
    Motifi625 – 6295"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    HOGENOMiHOG000246403.
    KOiK01870.
    OMAiRYVEGKW.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02003. Ile_tRNA_synth_type2.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR023586. Ile-tRNA-ligase_type2.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view]
    PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 2 hits.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O58792-1 [UniParc]FASTAAdd to Basket

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    MIKEPEFRDY TPGKLEEKIE EFWKENNIYQ KIKELRKNGP KYYFLDGPPY     50
    VSGAIHLGTA WNKIIKDMII RFRTMQGYNV WRQPGFDMHG LPIEVKVEQA 100
    LGLKTKKEIE EKIGVENFIK KCKEFALNNL KIMTEQFKML GIWMDWDDPY 150
    MTIKNEYIES AWFTLKKAWE KGLLEKDKRV LHWCPRCETA LAEHEVRGEY 200
    KLRKDPSIYV KFPIEGKENE YLLIWTTTPW TLPANLAVSA HPEYDYVKVK 250
    VEFNGKEEYW ILAKALVDKV LGEIGVKGEV IEEFKGRELE GLRYIHVLMD 300
    EYPRQKEFRE KYEWAHRIIL ADFVTLEEGT GLVHTAPGHG EEDFEVGKKY 350
    GLPIYSPVDD QGRYVEGKWK GIYVKEADPQ IIEHLKEKGY LVKAGEIEHK 400
    YPHCWRCKTP LIFRATDQWF LKVSKVKDRI IKENDEKVTW YPDWVKIRFD 450
    NGVRDSGDWV ISRQRYWGIP LPIWQSEDGE IYVVGSWREL VELAVAIEVN 500
    GERIDLPESY EEKLKVIEEK LGPEDLHRPY VDAFIIKVNG KEMRRVKDVV 550
    DVWFDSGIAS WASLGYPRNK ELFEKLWPAD FIVEGEDQVT KWFYSQQAAS 600
    VIAFDTVPYR RVAMHGYVLD EKGDKMSKSL GNIIRPEEVV EKAGRDTFRF 650
    YMLWATNPWE NLKFSWKGVE QVRRMLNILW NVYVLSATYM SLDNFDPRNV 700
    KVEELAFREE DKWILSRVNS LIREVENGIE TFYLTKATRA LYNFVVEDLS 750
    RWYVRLIRKR LWVEGDDPDK LAAYYTLWKV FDVLLRLMAP FTPYITEEIY 800
    QNLMRPFIGI ESVHMLDWPK VDESAVDEDL EKEMEFIRRI VEAGSAARQK 850
    ARIKLRYPVR KIIIETQDET VKKAVERLNY ILRDQLNAKE VVIGKVEREL 900
    TVKPNFAKVG PEFKGDSRLV AKWINEHGLE LYEKGEVDVE IEGKKFHLTR 950
    EHIIVEEKLP DFLVAEDFEG GRVYVDKTLT RELLAEGLAR EFVRRIQEMR 1000
    KRLDLDVNDR IVVTIETTDD NRELLQENLD YIMRETRAIE VRFEEAKGYV 1050
    VEWPEVQAKI GIEKVE 1066
    Length:1,066
    Mass (Da):125,941
    Last modified:August 1, 1998 - v1
    Checksum:i4C9444C82BB14018
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000001 Genomic DNA. Translation: BAA30164.1.
    PIRiF71100.
    RefSeqiNP_142976.1. NC_000961.1.
    WP_010885154.1. NC_000961.1.

    Genome annotation databases

    EnsemblBacteriaiBAA30164; BAA30164; BAA30164.
    GeneIDi1443389.
    KEGGipho:PH1065.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000001 Genomic DNA. Translation: BAA30164.1 .
    PIRi F71100.
    RefSeqi NP_142976.1. NC_000961.1.
    WP_010885154.1. NC_000961.1.

    3D structure databases

    ProteinModelPortali O58792.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 70601.PH1065.

    Proteomic databases

    PRIDEi O58792.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAA30164 ; BAA30164 ; BAA30164 .
    GeneIDi 1443389.
    KEGGi pho:PH1065.

    Phylogenomic databases

    eggNOGi COG0060.
    HOGENOMi HOG000246403.
    KOi K01870.
    OMAi RYVEGKW.

    Enzyme and pathway databases

    BioCyci PHOR70601:GJWR-1060-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02003. Ile_tRNA_synth_type2.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR023586. Ile-tRNA-ligase_type2.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view ]
    PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 2 hits.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3.

    Entry informationi

    Entry nameiSYI_PYRHO
    AccessioniPrimary (citable) accession number: O58792
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 30, 2000
    Last sequence update: August 1, 1998
    Last modified: October 1, 2014
    This is version 93 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3