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Protein

Aspartate--tRNA(Asp) ligase

Gene

aspS

Organism
Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the attachment of glutamate to tRNA(Asp) in a two-step reaction: aspartate is first activated by ATP to form Asp-AMP and then transferred to the acceptor end of tRNA(Asp).UniRule annotation

Catalytic activityi

ATP + L-aspartate + tRNA(Asp) = AMP + diphosphate + L-aspartyl-tRNA(Asp).UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 3 Mg2+ cations per subunit. The strongest magnesium site (Mg1) is bound to the beta- and gamma-phosphates of ATP and four water molecules complete its coordination sphere.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei85 – 851Important for tRNA discriminationUniRule annotation
Binding sitei170 – 1701AspartateUniRule annotation
Binding sitei214 – 2141AspartateUniRule annotation
Metal bindingi361 – 3611Magnesium 2UniRule annotation
Metal bindingi361 – 3611Magnesium 3UniRule annotation
Binding sitei361 – 3611ATPUniRule annotation
Metal bindingi364 – 3641Magnesium 2UniRule annotation
Binding sitei364 – 3641AspartateUniRule annotation
Binding sitei368 – 3681AspartateUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi214 – 2163ATPUniRule annotation
Nucleotide bindingi222 – 2243ATPUniRule annotation
Nucleotide bindingi409 – 4124ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciPHOR70601:GJWR-1013-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Aspartate--tRNA(Asp) ligaseUniRule annotation (EC:6.1.1.12UniRule annotation)
Alternative name(s):
Aspartyl-tRNA synthetaseUniRule annotation
Short name:
AspRSUniRule annotation
Discriminating aspartyl-tRNA synthetaseUniRule annotation
Short name:
D-AspRSUniRule annotation
Gene namesi
Name:aspSUniRule annotation
Ordered Locus Names:PH1020
OrganismiPyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Taxonomic identifieri70601 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
Proteomesi
  • UP000000752 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 438438Aspartate--tRNA(Asp) ligasePRO_0000111004Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

IntActiO58776. 1 interaction.
MINTiMINT-1501366.
STRINGi70601.PH1020.

Structurei

3D structure databases

ProteinModelPortaliO58776.
SMRiO58776. Positions 1-438.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni192 – 1954AspartateUniRule annotation

Sequence similaritiesi

Belongs to the class-II aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiarCOG00406. Archaea.
COG0017. LUCA.
HOGENOMiHOG000226032.
KOiK01876.
OMAiKPEICRA.

Family and domain databases

Gene3Di2.40.50.140. 1 hit.
HAMAPiMF_00044. Asp_tRNA_synth.
InterProiIPR004364. aa-tRNA-synt_II.
IPR018150. aa-tRNA-synt_II-like.
IPR006195. aa-tRNA-synth_II.
IPR004523. Asp-tRNA_synthase_arc.
IPR002312. Asp/Asn-tRNA-synth_IIb.
IPR012340. NA-bd_OB-fold.
IPR004365. NA-bd_OB_tRNA.
[Graphical view]
PANTHERiPTHR22594. PTHR22594. 1 hit.
PTHR22594:SF10. PTHR22594:SF10. 1 hit.
PfamiPF00152. tRNA-synt_2. 1 hit.
PF01336. tRNA_anti-codon. 1 hit.
[Graphical view]
PRINTSiPR01042. TRNASYNTHASP.
SUPFAMiSSF50249. SSF50249. 1 hit.
TIGRFAMsiTIGR00458. aspS_nondisc. 1 hit.
PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O58776-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLRTHYSNEI TEELNGKRVK VAGWVQEVKD LGGIKFIWIR DREGIVQVTA
60 70 80 90 100
PKKKVSQEIF KLIPKLNSED VIVVEGIVNF TPKAKLGFEV IPEKLEVISR
110 120 130 140 150
AKTPLPLDPT GKVKAELDTR LDNRFMDLRN PKVMAIFKIR SSVFRAVREF
160 170 180 190 200
FYSEGFIEIH TPKIIATATE GGTELFPLKY FERDAFLAQS PQLYKQMMMA
210 220 230 240 250
TGLDKVFEIA PIFRAEEHNT TRHLNEAWSI DAEMAFIENE GEVMDLLERL
260 270 280 290 300
ISYVINYVRE HNEKELKTLE FELNEPKRPF PRITYDKALE ILSDLGKEIP
310 320 330 340 350
WGEDIDTEGE KLLGKYMAEN EGADLYFIYR YPSEAKPFYI MKYDEKPEVC
360 370 380 390 400
RAFDLEYRGV EISSGGQREH RHDVLLEQIK EKGLNPKSFE FYLKAFEYGM
410 420 430
PPHGGFGLGA ERLIMRMLDI GNIREVILFP RDRRRLVP
Length:438
Mass (Da):50,869
Last modified:August 1, 1998 - v1
Checksum:i5B62A7B281A3B4E9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000001 Genomic DNA. Translation: BAA30117.1.
PIRiG71094.
RefSeqiWP_010885107.1. NC_000961.1.

Genome annotation databases

EnsemblBacteriaiBAA30117; BAA30117; BAA30117.
GeneIDi1443342.
KEGGipho:PH1020.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000001 Genomic DNA. Translation: BAA30117.1.
PIRiG71094.
RefSeqiWP_010885107.1. NC_000961.1.

3D structure databases

ProteinModelPortaliO58776.
SMRiO58776. Positions 1-438.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiO58776. 1 interaction.
MINTiMINT-1501366.
STRINGi70601.PH1020.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAA30117; BAA30117; BAA30117.
GeneIDi1443342.
KEGGipho:PH1020.

Phylogenomic databases

eggNOGiarCOG00406. Archaea.
COG0017. LUCA.
HOGENOMiHOG000226032.
KOiK01876.
OMAiKPEICRA.

Enzyme and pathway databases

BioCyciPHOR70601:GJWR-1013-MONOMER.

Family and domain databases

Gene3Di2.40.50.140. 1 hit.
HAMAPiMF_00044. Asp_tRNA_synth.
InterProiIPR004364. aa-tRNA-synt_II.
IPR018150. aa-tRNA-synt_II-like.
IPR006195. aa-tRNA-synth_II.
IPR004523. Asp-tRNA_synthase_arc.
IPR002312. Asp/Asn-tRNA-synth_IIb.
IPR012340. NA-bd_OB-fold.
IPR004365. NA-bd_OB_tRNA.
[Graphical view]
PANTHERiPTHR22594. PTHR22594. 1 hit.
PTHR22594:SF10. PTHR22594:SF10. 1 hit.
PfamiPF00152. tRNA-synt_2. 1 hit.
PF01336. tRNA_anti-codon. 1 hit.
[Graphical view]
PRINTSiPR01042. TRNASYNTHASP.
SUPFAMiSSF50249. SSF50249. 1 hit.
TIGRFAMsiTIGR00458. aspS_nondisc. 1 hit.
PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3.

Entry informationi

Entry nameiSYD_PYRHO
AccessioniPrimary (citable) accession number: O58776
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: August 1, 1998
Last modified: November 11, 2015
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.