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Protein

Tyrosine--tRNA ligase

Gene

tyrS

Organism
Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction: tyrosine is first activated by ATP to form Tyr-AMP and then transferred to the acceptor end of tRNA(Tyr).UniRule annotation

Catalytic activityi

ATP + L-tyrosine + tRNA(Tyr) = AMP + diphosphate + L-tyrosyl-tRNA(Tyr).UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei37TyrosineUniRule annotation1 Publication1
Binding sitei168TyrosineUniRule annotation1 Publication1
Binding sitei172TyrosineUniRule annotation1 Publication1
Binding sitei175TyrosineUniRule annotation1 Publication1
Binding sitei190TyrosineUniRule annotation1 Publication1
Binding sitei254ATPUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi6.1.1.1. 5244.

Names & Taxonomyi

Protein namesi
Recommended name:
Tyrosine--tRNA ligaseUniRule annotation (EC:6.1.1.1UniRule annotation)
Alternative name(s):
Tyrosyl-tRNA synthetaseUniRule annotation
Short name:
TyrRSUniRule annotation
Gene namesi
Name:tyrSUniRule annotation
Ordered Locus Names:PH1011
OrganismiPyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Taxonomic identifieri70601 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
Proteomesi
  • UP000000752 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002402691 – 375Tyrosine--tRNA ligaseAdd BLAST375

Interactioni

Subunit structurei

Homodimer.UniRule annotation1 Publication

Protein-protein interaction databases

STRINGi70601.PH1011.

Structurei

Secondary structure

1375
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi3 – 11Combined sources9
Beta strandi15 – 19Combined sources5
Helixi21 – 30Combined sources10
Beta strandi35 – 40Combined sources6
Helixi48 – 63Combined sources16
Beta strandi69 – 72Combined sources4
Helixi74 – 79Combined sources6
Helixi82 – 85Combined sources4
Helixi87 – 96Combined sources10
Helixi98 – 108Combined sources11
Helixi113 – 115Combined sources3
Beta strandi116 – 120Combined sources5
Helixi122 – 125Combined sources4
Helixi128 – 138Combined sources11
Helixi143 – 148Combined sources6
Helixi149 – 153Combined sources5
Helixi164 – 178Combined sources15
Beta strandi182 – 187Combined sources6
Helixi188 – 190Combined sources3
Helixi191 – 200Combined sources10
Helixi201 – 203Combined sources3
Beta strandi205 – 207Combined sources3
Beta strandi209 – 211Combined sources3
Beta strandi214 – 216Combined sources3
Beta strandi219 – 223Combined sources5
Beta strandi233 – 236Combined sources4
Helixi240 – 250Combined sources11
Helixi252 – 254Combined sources3
Helixi257 – 259Combined sources3
Helixi267 – 276Combined sources10
Helixi288 – 295Combined sources8
Turni296 – 298Combined sources3
Beta strandi299 – 302Combined sources4
Beta strandi305 – 307Combined sources3
Helixi311 – 313Combined sources3
Beta strandi317 – 321Combined sources5
Helixi322 – 330Combined sources9
Helixi336 – 350Combined sources15
Helixi352 – 360Combined sources9
Helixi363 – 370Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2CYCX-ray2.20A/B1-375[»]
ProteinModelPortaliO58739.
SMRiO58739.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO58739.

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi251 – 255"KMSKS" region5

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. TyrS type 4 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiarCOG01886. Archaea.
COG0162. LUCA.
HOGENOMiHOG000228238.
KOiK01866.
OMAiHVIAIEV.

Family and domain databases

CDDicd00805. TyrRS_core. 1 hit.
Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_02009. Tyr_tRNA_synth_type4. 1 hit.
InterProiIPR002305. aa-tRNA-synth_Ic.
IPR014729. Rossmann-like_a/b/a_fold.
IPR002307. Tyr-tRNA-ligase.
IPR023678. Tyr-tRNA-ligase_4.
IPR023617. Tyr-tRNA-ligase_arc/euk-type.
[Graphical view]
PfamiPF00579. tRNA-synt_1b. 1 hit.
[Graphical view]
PIRSFiPIRSF006588. TyrRS_arch_euk. 1 hit.

Sequencei

Sequence statusi: Complete.

O58739-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDIEERINLV LKKPTEEVLT VENLRHLFEI GAPLQHYIGF EISGYIHLGT
60 70 80 90 100
GLMAGAKIAD FQKAGIKTRV FLADWHSWIN DKLGGDLEVI QEVALKYFKV
110 120 130 140 150
GMEKSIEVMG GDPKKVEFVL ASEILEKGDY WQTVIDISKN VTLSRVMRSI
160 170 180 190 200
TIMGRQMGEA IDFAKLIYPM MQVADIFYQG VTIAHAGMDQ RKAHVIAIEV
210 220 230 240 250
AQKLRYHPIV HEGEKLKPVA VHHHLLLGLQ EPPKWPIESE EEFKEIKAQM
260 270 280 290 300
KMSKSKPYSA VFIHDSPEEI RQKLRKAFCP AREVRYNPVL DWVEYIIFRE
310 320 330 340 350
EPTEFTVHRP AKFGGDVTYT TFEELKRDFA EGKLHPLDLK NAVAEYLINL
360 370
LEPIRRYFEK HPEPLELMRS VKITR
Length:375
Mass (Da):43,276
Last modified:August 1, 1998 - v1
Checksum:i8A4BC945037A9F1B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000001 Genomic DNA. Translation: BAA30108.1.
PIRiF71093.
RefSeqiWP_010885097.1. NC_000961.1.

Genome annotation databases

EnsemblBacteriaiBAA30108; BAA30108; BAA30108.
GeneIDi1443332.
KEGGipho:PH1011.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000001 Genomic DNA. Translation: BAA30108.1.
PIRiF71093.
RefSeqiWP_010885097.1. NC_000961.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2CYCX-ray2.20A/B1-375[»]
ProteinModelPortaliO58739.
SMRiO58739.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi70601.PH1011.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAA30108; BAA30108; BAA30108.
GeneIDi1443332.
KEGGipho:PH1011.

Phylogenomic databases

eggNOGiarCOG01886. Archaea.
COG0162. LUCA.
HOGENOMiHOG000228238.
KOiK01866.
OMAiHVIAIEV.

Enzyme and pathway databases

BRENDAi6.1.1.1. 5244.

Miscellaneous databases

EvolutionaryTraceiO58739.

Family and domain databases

CDDicd00805. TyrRS_core. 1 hit.
Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_02009. Tyr_tRNA_synth_type4. 1 hit.
InterProiIPR002305. aa-tRNA-synth_Ic.
IPR014729. Rossmann-like_a/b/a_fold.
IPR002307. Tyr-tRNA-ligase.
IPR023678. Tyr-tRNA-ligase_4.
IPR023617. Tyr-tRNA-ligase_arc/euk-type.
[Graphical view]
PfamiPF00579. tRNA-synt_1b. 1 hit.
[Graphical view]
PIRSFiPIRSF006588. TyrRS_arch_euk. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiSYY_PYRHO
AccessioniPrimary (citable) accession number: O58739
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 13, 2006
Last sequence update: August 1, 1998
Last modified: November 30, 2016
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.