Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Proline--tRNA ligase

Gene

proS

Organism
Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction: proline is first activated by ATP to form Pro-AMP and then transferred to the acceptor end of tRNA(Pro).UniRule annotation

Catalytic activityi

ATP + L-proline + tRNA(Pro) = AMP + diphosphate + L-prolyl-tRNA(Pro).UniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciPHOR70601:GJWR-999-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Proline--tRNA ligaseUniRule annotation (EC:6.1.1.15UniRule annotation)
Alternative name(s):
Prolyl-tRNA synthetaseUniRule annotation
Short name:
ProRSUniRule annotation
Gene namesi
Name:proSUniRule annotation
Ordered Locus Names:PH1006
OrganismiPyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Taxonomic identifieri70601 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
Proteomesi
  • UP000000752 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 480480Proline--tRNA ligasePRO_0000249171Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi70601.PH1006.

Structurei

3D structure databases

ProteinModelPortaliO58734.
SMRiO58734. Positions 10-480.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

Consists of three domains: the N-terminal catalytic domain, the anticodon-binding domain and the C-terminal extension.UniRule annotation

Sequence similaritiesi

Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 3 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiarCOG00402. Archaea.
COG0442. LUCA.
HOGENOMiHOG000167538.
KOiK01881.
OMAiVYWGKAY.

Family and domain databases

CDDicd00778. ProRS_core_arch_euk. 1 hit.
Gene3Di3.30.110.30. 1 hit.
3.40.50.800. 1 hit.
HAMAPiMF_01571. Pro_tRNA_synth_type3. 1 hit.
InterProiIPR002314. aa-tRNA-synt_IIb.
IPR006195. aa-tRNA-synth_II.
IPR004154. Anticodon-bd.
IPR002316. Pro-tRNA-ligase_IIa.
IPR004499. Pro-tRNA-ligase_IIa_arc-type.
IPR016061. Pro-tRNA_ligase_II_C.
IPR017449. Pro-tRNA_synth_II.
IPR033721. ProRS_core_arch_euk.
[Graphical view]
PANTHERiPTHR11451:SF6. PTHR11451:SF6. 1 hit.
PfamiPF03129. HGTP_anticodon. 1 hit.
PF09180. ProRS-C_1. 1 hit.
PF00587. tRNA-synt_2b. 1 hit.
[Graphical view]
PRINTSiPR01046. TRNASYNTHPRO.
SMARTiSM00946. ProRS-C_1. 1 hit.
[Graphical view]
SUPFAMiSSF52954. SSF52954. 1 hit.
SSF64586. SSF64586. 1 hit.
TIGRFAMsiTIGR00408. proS_fam_I. 1 hit.
PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O58734-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVERKRWSEE FSEWFNEVIE EAGILDKRYP VKGMNVWLPY GLKIMRNIEK
60 70 80 90 100
FIHEEMERTG HQEVLFPALI PETEFKKEAE HIAGFEGEVF WVTHAGHEPL
110 120 130 140 150
DVRLVLRPTS ETAMYSMFAL WIRSHADLPF KVYQIVNVYR YETKHTRPLI
160 170 180 190 200
RVREISRFFE AHTAHADFED AERQIKEDLE IFDNLMRKLA LAYIISKRPE
210 220 230 240 250
WDKFPGAFYS LGAEVVMPDG RTLQIGTMHN YKQNFSKAYN ILYEKEDGTH
260 270 280 290 300
DYVHQTTFGM SERLLAAVIA IHGDDRGMVL PPTIAPIQVV IVPIPKKGSE
310 320 330 340 350
EEVYSYAKGI EEELRDAGIR VYLDLRDKRP GWKFYDWELK GVPVRVEVGP
360 370 380 390 400
IDVQNSTVVL ARRDKLEKIT VKREELVDKV RELFEDIMEF LYERANEWLE
410 420 430 440 450
SHIKRVDTLE EAKAVFEDRR GIVEIPWCGE ESCGLKMEEE LEAKMLGIPY
460 470 480
PEEKAKAPEG SKCPVCGREA KFIARFARTY
Length:480
Mass (Da):56,099
Last modified:January 1, 1999 - v2
Checksum:i706FF05E6BD27499
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000001 Genomic DNA. Translation: BAA30103.1.
PIRiA71093.
RefSeqiWP_010885093.1. NC_000961.1.

Genome annotation databases

EnsemblBacteriaiBAA30103; BAA30103; BAA30103.
GeneIDi1443328.
KEGGipho:PH1006.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000001 Genomic DNA. Translation: BAA30103.1.
PIRiA71093.
RefSeqiWP_010885093.1. NC_000961.1.

3D structure databases

ProteinModelPortaliO58734.
SMRiO58734. Positions 10-480.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi70601.PH1006.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAA30103; BAA30103; BAA30103.
GeneIDi1443328.
KEGGipho:PH1006.

Phylogenomic databases

eggNOGiarCOG00402. Archaea.
COG0442. LUCA.
HOGENOMiHOG000167538.
KOiK01881.
OMAiVYWGKAY.

Enzyme and pathway databases

BioCyciPHOR70601:GJWR-999-MONOMER.

Family and domain databases

CDDicd00778. ProRS_core_arch_euk. 1 hit.
Gene3Di3.30.110.30. 1 hit.
3.40.50.800. 1 hit.
HAMAPiMF_01571. Pro_tRNA_synth_type3. 1 hit.
InterProiIPR002314. aa-tRNA-synt_IIb.
IPR006195. aa-tRNA-synth_II.
IPR004154. Anticodon-bd.
IPR002316. Pro-tRNA-ligase_IIa.
IPR004499. Pro-tRNA-ligase_IIa_arc-type.
IPR016061. Pro-tRNA_ligase_II_C.
IPR017449. Pro-tRNA_synth_II.
IPR033721. ProRS_core_arch_euk.
[Graphical view]
PANTHERiPTHR11451:SF6. PTHR11451:SF6. 1 hit.
PfamiPF03129. HGTP_anticodon. 1 hit.
PF09180. ProRS-C_1. 1 hit.
PF00587. tRNA-synt_2b. 1 hit.
[Graphical view]
PRINTSiPR01046. TRNASYNTHPRO.
SMARTiSM00946. ProRS-C_1. 1 hit.
[Graphical view]
SUPFAMiSSF52954. SSF52954. 1 hit.
SSF64586. SSF64586. 1 hit.
TIGRFAMsiTIGR00408. proS_fam_I. 1 hit.
PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSYP_PYRHO
AccessioniPrimary (citable) accession number: O58734
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 5, 2006
Last sequence update: January 1, 1999
Last modified: September 7, 2016
This is version 107 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.