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Protein

Proline--tRNA ligase

Gene

proS

Organism
Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction: proline is first activated by ATP to form Pro-AMP and then transferred to the acceptor end of tRNA(Pro).UniRule annotation

Catalytic activityi

ATP + L-proline + tRNA(Pro) = AMP + diphosphate + L-prolyl-tRNA(Pro).UniRule annotation

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciPHOR70601:G1G39-980-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Proline--tRNA ligaseUniRule annotation (EC:6.1.1.15UniRule annotation)
Alternative name(s):
Prolyl-tRNA synthetaseUniRule annotation
Short name:
ProRSUniRule annotation
Gene namesi
Name:proSUniRule annotation
Ordered Locus Names:PH1006
OrganismiPyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Taxonomic identifieri70601 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
Proteomesi
  • UP000000752 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002491711 – 480Proline--tRNA ligaseAdd BLAST480

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi70601.PH1006

Structurei

3D structure databases

ProteinModelPortaliO58734
SMRiO58734
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

Consists of three domains: the N-terminal catalytic domain, the anticodon-binding domain and the C-terminal extension.UniRule annotation

Sequence similaritiesi

Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 3 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiarCOG00402 Archaea
COG0442 LUCA
HOGENOMiHOG000167538
KOiK01881
OMAiEVYWVTH
OrthoDBiPOG093Z01RZ

Family and domain databases

CDDicd00778 ProRS_core_arch_euk, 1 hit
Gene3Di3.30.110.30, 1 hit
3.40.50.800, 1 hit
HAMAPiMF_01571 Pro_tRNA_synth_type3, 1 hit
InterProiView protein in InterPro
IPR002314 aa-tRNA-synt_IIb
IPR006195 aa-tRNA-synth_II
IPR004154 Anticodon-bd
IPR036621 Anticodon-bd_dom_sf
IPR002316 Pro-tRNA-ligase_IIa
IPR004499 Pro-tRNA-ligase_IIa_arc-type
IPR016061 Pro-tRNA_ligase_II_C
IPR017449 Pro-tRNA_synth_II
IPR033721 ProRS_core_arch_euk
PfamiView protein in Pfam
PF03129 HGTP_anticodon, 1 hit
PF09180 ProRS-C_1, 1 hit
PF00587 tRNA-synt_2b, 1 hit
PRINTSiPR01046 TRNASYNTHPRO
SMARTiView protein in SMART
SM00946 ProRS-C_1, 1 hit
SUPFAMiSSF64586 SSF64586, 1 hit
TIGRFAMsiTIGR00408 proS_fam_I, 1 hit
PROSITEiView protein in PROSITE
PS50862 AA_TRNA_LIGASE_II, 1 hit

Sequencei

Sequence statusi: Complete.

O58734-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVERKRWSEE FSEWFNEVIE EAGILDKRYP VKGMNVWLPY GLKIMRNIEK
60 70 80 90 100
FIHEEMERTG HQEVLFPALI PETEFKKEAE HIAGFEGEVF WVTHAGHEPL
110 120 130 140 150
DVRLVLRPTS ETAMYSMFAL WIRSHADLPF KVYQIVNVYR YETKHTRPLI
160 170 180 190 200
RVREISRFFE AHTAHADFED AERQIKEDLE IFDNLMRKLA LAYIISKRPE
210 220 230 240 250
WDKFPGAFYS LGAEVVMPDG RTLQIGTMHN YKQNFSKAYN ILYEKEDGTH
260 270 280 290 300
DYVHQTTFGM SERLLAAVIA IHGDDRGMVL PPTIAPIQVV IVPIPKKGSE
310 320 330 340 350
EEVYSYAKGI EEELRDAGIR VYLDLRDKRP GWKFYDWELK GVPVRVEVGP
360 370 380 390 400
IDVQNSTVVL ARRDKLEKIT VKREELVDKV RELFEDIMEF LYERANEWLE
410 420 430 440 450
SHIKRVDTLE EAKAVFEDRR GIVEIPWCGE ESCGLKMEEE LEAKMLGIPY
460 470 480
PEEKAKAPEG SKCPVCGREA KFIARFARTY
Length:480
Mass (Da):56,099
Last modified:January 1, 1999 - v2
Checksum:i706FF05E6BD27499
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000001 Genomic DNA Translation: BAA30103.1
PIRiA71093
RefSeqiWP_010885093.1, NC_000961.1

Genome annotation databases

EnsemblBacteriaiBAA30103; BAA30103; BAA30103
GeneIDi1443328
KEGGipho:PH1006

Similar proteinsi

Entry informationi

Entry nameiSYP_PYRHO
AccessioniPrimary (citable) accession number: O58734
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 5, 2006
Last sequence update: January 1, 1999
Last modified: April 25, 2018
This is version 118 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health