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Protein
Submitted name:

180aa long hypothetical pyrazinamidase/nicotinamidase

Gene

PH0999

Organism
Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Status
Unreviewed-Annotation score: Annotation score: 1 out of 5-Experimental evidence at protein leveli

Functioni

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei10 – 101Proton donor/acceptorCombined sources
Metal bindingi52 – 521ZincCombined sources
Metal bindingi54 – 541Zinc; via tele nitrogenCombined sources
Metal bindingi71 – 711Zinc; via tele nitrogenCombined sources
Sitei94 – 941Important for catalytic activityCombined sources
Binding sitei129 – 1291Substrate; via amide nitrogenCombined sources
Active sitei133 – 1331NucleophileCombined sources

GO - Molecular functioni

  1. catalytic activity Source: InterPro
  2. metal ion binding Source: UniProtKB-KW
Complete GO annotation...

Keywords - Ligandi

Metal-bindingCombined sources, ZincCombined sources

Enzyme and pathway databases

BioCyciPHOR70601:GJWR-991-MONOMER.

Names & Taxonomyi

Protein namesi
Submitted name:
180aa long hypothetical pyrazinamidase/nicotinamidaseImported
Gene namesi
Ordered Locus Names:PH0999Imported
OrganismiPyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)Imported
Taxonomic identifieri70601 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
ProteomesiUP000000752: Chromosome

Interactioni

Protein-protein interaction databases

STRINGi70601.PH0999.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1ILWX-ray2.05A1-180[»]
1IM5X-ray1.65A1-180[»]
ProteinModelPortaliO58727.
SMRiO58727. Positions 2-180.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO58727.

Family & Domainsi

Phylogenomic databases

eggNOGiCOG1335.
HOGENOMiHOG000078666.
KOiK08281.
OMAiDKEAYSG.

Family and domain databases

Gene3Di3.40.50.850. 1 hit.
InterProiIPR000868. Isochorismatase-like.
[Graphical view]
PfamiPF00857. Isochorismatase. 1 hit.
[Graphical view]
SUPFAMiSSF52499. SSF52499. 1 hit.

Sequencei

Sequence statusi: Complete.

O58727-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPEEALIVVD MQRDFMPGGA LPVPEGDKII PKVNEYIRKF KEKGALIVAT
60 70 80 90 100
RDWHPENHIS FRERGGPWPR HCVQNTPGAE FVVDLPEDAV IISKATEPDK
110 120 130 140 150
EAYSGFEGTD LAKILRGNGV KRVYICGVAT EYCVRATALD ALKHGFEVYL
160 170 180
LRDAVKGIKP EDEERALEEM KSRGIKIVQF
Length:180
Mass (Da):20,192
Last modified:August 1, 1998 - v1
Checksum:i7FFEE8E90C0EE89C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000001 Genomic DNA. Translation: BAA30096.1.
PIRiB71092.
RefSeqiNP_142913.1. NC_000961.1.
WP_010885086.1. NC_000961.1.

Genome annotation databases

EnsemblBacteriaiBAA30096; BAA30096; BAA30096.
GeneIDi1443321.
KEGGipho:PH0999.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000001 Genomic DNA. Translation: BAA30096.1.
PIRiB71092.
RefSeqiNP_142913.1. NC_000961.1.
WP_010885086.1. NC_000961.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1ILWX-ray2.05A1-180[»]
1IM5X-ray1.65A1-180[»]
ProteinModelPortaliO58727.
SMRiO58727. Positions 2-180.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi70601.PH0999.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAA30096; BAA30096; BAA30096.
GeneIDi1443321.
KEGGipho:PH0999.

Phylogenomic databases

eggNOGiCOG1335.
HOGENOMiHOG000078666.
KOiK08281.
OMAiDKEAYSG.

Enzyme and pathway databases

BioCyciPHOR70601:GJWR-991-MONOMER.

Miscellaneous databases

EvolutionaryTraceiO58727.

Family and domain databases

Gene3Di3.40.50.850. 1 hit.
InterProiIPR000868. Isochorismatase-like.
[Graphical view]
PfamiPF00857. Isochorismatase. 1 hit.
[Graphical view]
SUPFAMiSSF52499. SSF52499. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3Imported.
  2. "Crystal structure and mechanism of catalysis of a pyrazinamidase from Pyrococcus horikoshii."
    Du X., Wang W., Kim R., Yakota H., Nguyen H., Kim S.H.
    Biochemistry 40:14166-14172(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.65 ANGSTROMS) IN COMPLEX WITH ZINC, ACTIVE SITE.

Entry informationi

Entry nameiO58727_PYRHO
AccessioniPrimary (citable) accession number: O58727
Entry historyi
Integrated into UniProtKB/TrEMBL: August 1, 1998
Last sequence update: August 1, 1998
Last modified: February 4, 2015
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

3D-structureCombined sources, Complete proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.