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O58727

- O58727_PYRHO

UniProt

O58727 - O58727_PYRHO

Protein
Submitted name:

180aa long hypothetical pyrazinamidase/nicotinamidase

Gene

PH0999

Organism
Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Status
Unreviewed - Annotation score: 1 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 80 (01 Oct 2014)
      Sequence version 1 (01 Aug 1998)
      Previous versions | rss
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    Functioni

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei10 – 101Proton donor/acceptorImported
    Metal bindingi52 – 521ZincImported
    Metal bindingi54 – 541Zinc; via tele nitrogenImported
    Metal bindingi71 – 711Zinc; via tele nitrogenImported
    Sitei94 – 941Important for catalytic activityImported
    Binding sitei129 – 1291Substrate; via amide nitrogenImported
    Active sitei133 – 1331NucleophileImported

    GO - Molecular functioni

    1. catalytic activity Source: InterPro
    2. metal ion binding Source: UniProtKB-KW

    Keywords - Ligandi

    Metal-bindingImported, ZincImported

    Enzyme and pathway databases

    BioCyciPHOR70601:GJWR-991-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Submitted name:
    180aa long hypothetical pyrazinamidase/nicotinamidaseImported
    Gene namesi
    Ordered Locus Names:PH0999Imported
    OrganismiPyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)Imported
    Taxonomic identifieri70601 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
    ProteomesiUP000000752: Chromosome

    Interactioni

    Protein-protein interaction databases

    STRINGi70601.PH0999.

    Structurei

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1ILWX-ray2.05A1-180[»]
    1IM5X-ray1.65A1-180[»]
    ProteinModelPortaliO58727.
    SMRiO58727. Positions 2-180.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiO58727.

    Family & Domainsi

    Phylogenomic databases

    eggNOGiCOG1335.
    HOGENOMiHOG000078666.
    KOiK08281.
    OMAiERSEACI.

    Family and domain databases

    Gene3Di3.40.50.850. 1 hit.
    InterProiIPR000868. Isochorismatase-like.
    [Graphical view]
    PfamiPF00857. Isochorismatase. 1 hit.
    [Graphical view]
    SUPFAMiSSF52499. SSF52499. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    O58727-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPEEALIVVD MQRDFMPGGA LPVPEGDKII PKVNEYIRKF KEKGALIVAT    50
    RDWHPENHIS FRERGGPWPR HCVQNTPGAE FVVDLPEDAV IISKATEPDK 100
    EAYSGFEGTD LAKILRGNGV KRVYICGVAT EYCVRATALD ALKHGFEVYL 150
    LRDAVKGIKP EDEERALEEM KSRGIKIVQF 180
    Length:180
    Mass (Da):20,192
    Last modified:August 1, 1998 - v1
    Checksum:i7FFEE8E90C0EE89C
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000001 Genomic DNA. Translation: BAA30096.1.
    PIRiB71092.
    RefSeqiNP_142913.1. NC_000961.1.
    WP_010885086.1. NC_000961.1.

    Genome annotation databases

    EnsemblBacteriaiBAA30096; BAA30096; BAA30096.
    GeneIDi1443321.
    KEGGipho:PH0999.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000001 Genomic DNA. Translation: BAA30096.1 .
    PIRi B71092.
    RefSeqi NP_142913.1. NC_000961.1.
    WP_010885086.1. NC_000961.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1ILW X-ray 2.05 A 1-180 [» ]
    1IM5 X-ray 1.65 A 1-180 [» ]
    ProteinModelPortali O58727.
    SMRi O58727. Positions 2-180.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 70601.PH0999.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAA30096 ; BAA30096 ; BAA30096 .
    GeneIDi 1443321.
    KEGGi pho:PH0999.

    Phylogenomic databases

    eggNOGi COG1335.
    HOGENOMi HOG000078666.
    KOi K08281.
    OMAi ERSEACI.

    Enzyme and pathway databases

    BioCyci PHOR70601:GJWR-991-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei O58727.

    Family and domain databases

    Gene3Di 3.40.50.850. 1 hit.
    InterProi IPR000868. Isochorismatase-like.
    [Graphical view ]
    Pfami PF00857. Isochorismatase. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52499. SSF52499. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3Imported.
    2. "Crystal structure and mechanism of catalysis of a pyrazinamidase from Pyrococcus horikoshii."
      Du X., Wang W., Kim R., Yakota H., Nguyen H., Kim S.H.
      Biochemistry 40:14166-14172(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.65 ANGSTROMS) IN COMPLEX WITH ZINC, ACTIVE SITE.

    Entry informationi

    Entry nameiO58727_PYRHO
    AccessioniPrimary (citable) accession number: O58727
    Entry historyi
    Integrated into UniProtKB/TrEMBL: August 1, 1998
    Last sequence update: August 1, 1998
    Last modified: October 1, 2014
    This is version 80 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiUnreviewed (UniProtKB/TrEMBL)

    Miscellaneousi

    Keywords - Technical termi

    3D-structureImported, Complete proteomeImported

    External Data

    Dasty 3