Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Methionine--tRNA ligase

Gene

metG

Organism
Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation.UniRule annotation

Catalytic activityi

ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi143ZincUniRule annotation1
Metal bindingi146ZincUniRule annotation1
Metal bindingi156ZincUniRule annotation1
Metal bindingi159ZincUniRule annotation1
Binding sitei347ATPUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase, RNA-binding, tRNA-binding
Biological processProtein biosynthesis
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciPHOR70601:G1G39-968-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Methionine--tRNA ligaseUniRule annotation (EC:6.1.1.10UniRule annotation)
Alternative name(s):
Methionyl-tRNA synthetaseUniRule annotation
Short name:
MetRSUniRule annotation
Gene namesi
Name:metGUniRule annotation
Ordered Locus Names:PH0993
OrganismiPyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Taxonomic identifieri70601 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
Proteomesi
  • UP000000752 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001391971 – 723Methionine--tRNA ligaseAdd BLAST723

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi70601.PH0993

Structurei

3D structure databases

ProteinModelPortaliO58721
SMRiO58721
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini623 – 723tRNA-bindingUniRule annotationAdd BLAST101

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi11 – 21"HIGH" regionAdd BLAST11
Motifi344 – 348"KMSKS" region5

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiarCOG00810 Archaea
COG0073 LUCA
COG0143 LUCA
HOGENOMiHOG000200402
KOiK01874
OMAiTYVWFDA
OrthoDBiPOG093Z0HP9

Family and domain databases

CDDicd00814 MetRS_core, 1 hit
Gene3Di3.40.50.620, 2 hits
HAMAPiMF_00098 Met_tRNA_synth_type1, 1 hit
InterProiView protein in InterPro
IPR001412 aa-tRNA-synth_I_CS
IPR013155 M/V/L/I-tRNA-synth_anticd-bd
IPR004495 Met-tRNA-synth_bsu_C
IPR023458 Met-tRNA_ligase_1
IPR014758 Met-tRNA_synth
IPR015413 Methionyl/Leucyl_tRNA_Synth
IPR033911 MetRS_core
IPR029038 MetRS_Zn
IPR012340 NA-bd_OB-fold
IPR014729 Rossmann-like_a/b/a_fold
IPR002547 tRNA-bd_dom
IPR009080 tRNAsynth_Ia_anticodon-bd
PfamiView protein in Pfam
PF08264 Anticodon_1, 1 hit
PF09334 tRNA-synt_1g, 1 hit
PF01588 tRNA_bind, 1 hit
PRINTSiPR01041 TRNASYNTHMET
SUPFAMiSSF47323 SSF47323, 1 hit
SSF50249 SSF50249, 1 hit
SSF57770 SSF57770, 1 hit
TIGRFAMsiTIGR00398 metG, 1 hit
TIGR00399 metG_C_term, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit
PS50886 TRBD, 1 hit

Sequencei

Sequence statusi: Complete.

O58721-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVRYMVTSAL PYANGPIHAG HLAGAYLPAD IFVRYLRLKG EDVVFICGTD
60 70 80 90 100
EHGTPISFRA LKEGRSPREI VDEFHEHIKI TFQRAKISFD FFGRTELPIH
110 120 130 140 150
YKLSQQFFLK AYENGHLVKK VTKQAYCEHD KMFLPDRFVI GTCPFCGAEN
160 170 180 190 200
QRGDQCEVCG RPLTPEILIN PRCALCGRPI SFRESAHYYI KMQDFAEKLK
210 220 230 240 250
RWIENQPWKP NVKNMVLKWI EEGLEERAIT RDLNWGIPVP LDEEDMKNKV
260 270 280 290 300
LYVWFEAPIG YISITMEYFK RLGKPNEWKK YWLNIDSETR VIHFIGKDNI
310 320 330 340 350
PFHAIFWPAF LMAYGKYKDE EVEAEWNLPY DIPANEYLTL EGKKFSTSRN
360 370 380 390 400
WAIWVHEFLD VFPADYLRYY LTTIMPETRD SDFSFAEFKT RINEELVNNL
410 420 430 440 450
GNFVHRAMTF VNRYFDGIVP ERGELDELDR QALEEIEKAF EEVGELIMNY
460 470 480 490 500
RFKDALKRVM ELASFGNKYF DHKQPWKTAK EDRARTGTTV NISLQIVKAL
510 520 530 540 550
GILLEPFLPD ASEKIWHLLN LDEVKKWKFK ELPAGHRVRK AEILFKKVTD
560 570 580 590 600
EQIIYFILNY MGRNNPEGAK MLLEKYYKRE DVIKVAKEKF GEESKIILKR
610 620 630 640 650
IYKDIKLEEG KEEKEMEYVK FEDFAKLDLR VGKIIEVKDH PNADKLYIVK
660 670 680 690 700
VDLGKEVRTL VAGLKKYYKP EELLNKYVII VANLEPKKLR GVESQGMLLA
710 720
ADDGENVALL MPDKEVKLGA KVR
Length:723
Mass (Da):84,743
Last modified:August 1, 1998 - v1
Checksum:iF839FDBCE7DC09DC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000001 Genomic DNA Translation: BAA30090.1
PIRiD71091
RefSeqiWP_010885082.1, NC_000961.1

Genome annotation databases

EnsemblBacteriaiBAA30090; BAA30090; BAA30090
GeneIDi1443317
KEGGipho:PH0993

Similar proteinsi

Entry informationi

Entry nameiSYM_PYRHO
AccessioniPrimary (citable) accession number: O58721
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: August 1, 1998
Last modified: April 25, 2018
This is version 133 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

Cookie policy

We would like to use anonymized google analytics cookies to gather statistics on how uniprot.org is used in aggregate. Learn more

UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health