ID   SYL_PYRHO               Reviewed;         967 AA.
AC   O58698;
DT   30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1998, sequence version 1.
DT   27-MAR-2024, entry version 149.
DE   RecName: Full=Leucine--tRNA ligase {ECO:0000255|HAMAP-Rule:MF_00049};
DE            EC=6.1.1.4 {ECO:0000255|HAMAP-Rule:MF_00049};
DE   AltName: Full=Leucyl-tRNA synthetase {ECO:0000255|HAMAP-Rule:MF_00049};
DE            Short=LeuRS {ECO:0000255|HAMAP-Rule:MF_00049};
GN   Name=leuS {ECO:0000255|HAMAP-Rule:MF_00049}; OrderedLocusNames=PH0965;
OS   Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC
OS   100139 / OT-3).
OC   Archaea; Euryarchaeota; Thermococci; Thermococcales; Thermococcaceae;
OC   Pyrococcus.
OX   NCBI_TaxID=70601;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3;
RX   PubMed=9679194; DOI=10.1093/dnares/5.2.55;
RA   Kawarabayasi Y., Sawada M., Horikawa H., Haikawa Y., Hino Y., Yamamoto S.,
RA   Sekine M., Baba S., Kosugi H., Hosoyama A., Nagai Y., Sakai M., Ogura K.,
RA   Otsuka R., Nakazawa H., Takamiya M., Ohfuku Y., Funahashi T., Tanaka T.,
RA   Kudoh Y., Yamazaki J., Kushida N., Oguchi A., Aoki K., Yoshizawa T.,
RA   Nakamura Y., Robb F.T., Horikoshi K., Masuchi Y., Shizuya H., Kikuchi H.;
RT   "Complete sequence and gene organization of the genome of a hyper-
RT   thermophilic archaebacterium, Pyrococcus horikoshii OT3.";
RL   DNA Res. 5:55-76(1998).
RN   [2]
RP   X-RAY CRYSTALLOGRAPHY (3.21 ANGSTROMS).
RG   RIKEN structural genomics initiative (RSGI);
RT   "The crystal structure of leucyl-tRNA synthetase and tRNA(leucine)
RT   complex.";
RL   Submitted (SEP-2005) to the PDB data bank.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-
CC         tRNA(Leu); Xref=Rhea:RHEA:11688, Rhea:RHEA-COMP:9613, Rhea:RHEA-
CC         COMP:9622, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57427,
CC         ChEBI:CHEBI:78442, ChEBI:CHEBI:78494, ChEBI:CHEBI:456215; EC=6.1.1.4;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00049};
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00049}.
CC   -!- SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase family.
CC       {ECO:0000255|HAMAP-Rule:MF_00049}.
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DR   EMBL; BA000001; BAA30062.1; -; Genomic_DNA.
DR   PIR; H71087; H71087.
DR   RefSeq; WP_010885055.1; NC_000961.1.
DR   PDB; 1WKB; X-ray; 2.05 A; A=1-810.
DR   PDB; 1WZ2; X-ray; 3.21 A; A/B=1-967.
DR   PDBsum; 1WKB; -.
DR   PDBsum; 1WZ2; -.
DR   AlphaFoldDB; O58698; -.
DR   SMR; O58698; -.
DR   STRING; 70601.gene:9377920; -.
DR   EnsemblBacteria; BAA30062; BAA30062; BAA30062.
DR   GeneID; 1443290; -.
DR   KEGG; pho:PH0965; -.
DR   eggNOG; arCOG00809; Archaea.
DR   OrthoDB; 23906at2157; -.
DR   BRENDA; 6.1.1.4; 5244.
DR   EvolutionaryTrace; O58698; -.
DR   Proteomes; UP000000752; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0002161; F:aminoacyl-tRNA editing activity; IEA:InterPro.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004823; F:leucine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006429; P:leucyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR   CDD; cd07959; Anticodon_Ia_Leu_AEc; 1.
DR   CDD; cd00812; LeuRS_core; 1.
DR   Gene3D; 3.30.2320.20; Class I aminoacyl-tRNA synthetases (RS); 1.
DR   Gene3D; 3.40.50.620; HUPs; 1.
DR   Gene3D; 1.10.10.720; leucyl-tRNA synthetase; 1.
DR   Gene3D; 3.90.740.10; Valyl/Leucyl/Isoleucyl-tRNA synthetase, editing domain; 1.
DR   HAMAP; MF_00049_A; Leu_tRNA_synth_A; 1.
DR   InterPro; IPR001412; aa-tRNA-synth_I_CS.
DR   InterPro; IPR002300; aa-tRNA-synth_Ia.
DR   InterPro; IPR020791; Leu-tRNA-lgase_arc.
DR   InterPro; IPR004493; Leu-tRNA-synth_Ia_arc/euk.
DR   InterPro; IPR013155; M/V/L/I-tRNA-synth_anticd-bd.
DR   InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR   InterPro; IPR009080; tRNAsynth_Ia_anticodon-bd.
DR   InterPro; IPR009008; Val/Leu/Ile-tRNA-synth_edit.
DR   NCBIfam; TIGR00395; leuS_arch; 1.
DR   PANTHER; PTHR45794:SF1; LEUCINE--TRNA LIGASE, CYTOPLASMIC; 1.
DR   PANTHER; PTHR45794; LEUCYL-TRNA SYNTHETASE; 1.
DR   Pfam; PF08264; Anticodon_1; 1.
DR   Pfam; PF00133; tRNA-synt_1; 1.
DR   SUPFAM; SSF47323; Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases; 1.
DR   SUPFAM; SSF52374; Nucleotidylyl transferase; 1.
DR   SUPFAM; SSF50677; ValRS/IleRS/LeuRS editing domain; 1.
DR   PROSITE; PS00178; AA_TRNA_LIGASE_I; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW   Nucleotide-binding; Protein biosynthesis.
FT   CHAIN           1..967
FT                   /note="Leucine--tRNA ligase"
FT                   /id="PRO_0000152140"
FT   MOTIF           43..53
FT                   /note="'HIGH' region"
FT   MOTIF           650..654
FT                   /note="'KMSKS' region"
FT   BINDING         653
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00049"
FT   HELIX           7..19
FT                   /evidence="ECO:0007829|PDB:1WKB"
FT   TURN            20..23
FT                   /evidence="ECO:0007829|PDB:1WKB"
FT   HELIX           27..29
FT                   /evidence="ECO:0007829|PDB:1WKB"
FT   HELIX           32..34
FT                   /evidence="ECO:0007829|PDB:1WKB"
FT   STRAND          35..40
FT                   /evidence="ECO:0007829|PDB:1WKB"
FT   HELIX           51..70
FT                   /evidence="ECO:0007829|PDB:1WKB"
FT   STRAND          73..75
FT                   /evidence="ECO:0007829|PDB:1WKB"
FT   HELIX           86..95
FT                   /evidence="ECO:0007829|PDB:1WKB"
FT   HELIX           99..107
FT                   /evidence="ECO:0007829|PDB:1WKB"
FT   HELIX           113..117
FT                   /evidence="ECO:0007829|PDB:1WKB"
FT   HELIX           118..120
FT                   /evidence="ECO:0007829|PDB:1WKB"
FT   HELIX           122..139
FT                   /evidence="ECO:0007829|PDB:1WKB"
FT   HELIX           146..148
FT                   /evidence="ECO:0007829|PDB:1WKB"
FT   HELIX           157..172
FT                   /evidence="ECO:0007829|PDB:1WKB"
FT   STRAND          176..186
FT                   /evidence="ECO:0007829|PDB:1WKB"
FT   TURN            187..190
FT                   /evidence="ECO:0007829|PDB:1WKB"
FT   STRAND          195..197
FT                   /evidence="ECO:0007829|PDB:1WZ2"
FT   STRAND          206..215
FT                   /evidence="ECO:0007829|PDB:1WKB"
FT   STRAND          219..221
FT                   /evidence="ECO:0007829|PDB:1WZ2"
FT   STRAND          224..230
FT                   /evidence="ECO:0007829|PDB:1WKB"
FT   HELIX           232..237
FT                   /evidence="ECO:0007829|PDB:1WKB"
FT   STRAND          240..243
FT                   /evidence="ECO:0007829|PDB:1WKB"
FT   STRAND          245..247
FT                   /evidence="ECO:0007829|PDB:1WZ2"
FT   STRAND          249..256
FT                   /evidence="ECO:0007829|PDB:1WKB"
FT   STRAND          259..266
FT                   /evidence="ECO:0007829|PDB:1WKB"
FT   HELIX           267..273
FT                   /evidence="ECO:0007829|PDB:1WKB"
FT   STRAND          279..285
FT                   /evidence="ECO:0007829|PDB:1WKB"
FT   TURN            287..293
FT                   /evidence="ECO:0007829|PDB:1WKB"
FT   STRAND          295..297
FT                   /evidence="ECO:0007829|PDB:1WKB"
FT   STRAND          299..301
FT                   /evidence="ECO:0007829|PDB:1WKB"
FT   STRAND          304..309
FT                   /evidence="ECO:0007829|PDB:1WKB"
FT   STRAND          315..317
FT                   /evidence="ECO:0007829|PDB:1WZ2"
FT   STRAND          322..324
FT                   /evidence="ECO:0007829|PDB:1WKB"
FT   TURN            326..328
FT                   /evidence="ECO:0007829|PDB:1WKB"
FT   HELIX           330..336
FT                   /evidence="ECO:0007829|PDB:1WKB"
FT   HELIX           380..382
FT                   /evidence="ECO:0007829|PDB:1WKB"
FT   HELIX           394..406
FT                   /evidence="ECO:0007829|PDB:1WKB"
FT   HELIX           413..415
FT                   /evidence="ECO:0007829|PDB:1WKB"
FT   STRAND          417..419
FT                   /evidence="ECO:0007829|PDB:1WZ2"
FT   HELIX           420..432
FT                   /evidence="ECO:0007829|PDB:1WKB"
FT   TURN            433..435
FT                   /evidence="ECO:0007829|PDB:1WKB"
FT   STRAND          436..446
FT                   /evidence="ECO:0007829|PDB:1WKB"
FT   STRAND          451..455
FT                   /evidence="ECO:0007829|PDB:1WZ2"
FT   STRAND          457..467
FT                   /evidence="ECO:0007829|PDB:1WKB"
FT   HELIX           472..483
FT                   /evidence="ECO:0007829|PDB:1WKB"
FT   STRAND          485..489
FT                   /evidence="ECO:0007829|PDB:1WKB"
FT   HELIX           490..492
FT                   /evidence="ECO:0007829|PDB:1WKB"
FT   HELIX           493..502
FT                   /evidence="ECO:0007829|PDB:1WKB"
FT   STRAND          509..515
FT                   /evidence="ECO:0007829|PDB:1WKB"
FT   STRAND          521..526
FT                   /evidence="ECO:0007829|PDB:1WKB"
FT   TURN            528..530
FT                   /evidence="ECO:0007829|PDB:1WKB"
FT   STRAND          531..534
FT                   /evidence="ECO:0007829|PDB:1WKB"
FT   HELIX           535..550
FT                   /evidence="ECO:0007829|PDB:1WKB"
FT   HELIX           556..558
FT                   /evidence="ECO:0007829|PDB:1WKB"
FT   HELIX           561..569
FT                   /evidence="ECO:0007829|PDB:1WKB"
FT   HELIX           574..584
FT                   /evidence="ECO:0007829|PDB:1WKB"
FT   HELIX           588..601
FT                   /evidence="ECO:0007829|PDB:1WKB"
FT   STRAND          605..610
FT                   /evidence="ECO:0007829|PDB:1WKB"
FT   HELIX           611..613
FT                   /evidence="ECO:0007829|PDB:1WKB"
FT   TURN            614..616
FT                   /evidence="ECO:0007829|PDB:1WKB"
FT   HELIX           617..628
FT                   /evidence="ECO:0007829|PDB:1WKB"
FT   HELIX           631..633
FT                   /evidence="ECO:0007829|PDB:1WKB"
FT   STRAND          637..641
FT                   /evidence="ECO:0007829|PDB:1WKB"
FT   STRAND          644..646
FT                   /evidence="ECO:0007829|PDB:1WKB"
FT   TURN            653..656
FT                   /evidence="ECO:0007829|PDB:1WKB"
FT   HELIX           661..668
FT                   /evidence="ECO:0007829|PDB:1WKB"
FT   HELIX           670..680
FT                   /evidence="ECO:0007829|PDB:1WKB"
FT   STRAND          687..689
FT                   /evidence="ECO:0007829|PDB:1WKB"
FT   HELIX           691..712
FT                   /evidence="ECO:0007829|PDB:1WKB"
FT   HELIX           724..745
FT                   /evidence="ECO:0007829|PDB:1WKB"
FT   HELIX           749..756
FT                   /evidence="ECO:0007829|PDB:1WKB"
FT   HELIX           758..770
FT                   /evidence="ECO:0007829|PDB:1WKB"
FT   TURN            771..773
FT                   /evidence="ECO:0007829|PDB:1WKB"
FT   HELIX           777..794
FT                   /evidence="ECO:0007829|PDB:1WKB"
FT   TURN            795..797
FT                   /evidence="ECO:0007829|PDB:1WKB"
FT   HELIX           799..808
FT                   /evidence="ECO:0007829|PDB:1WKB"
FT   HELIX           815..817
FT                   /evidence="ECO:0007829|PDB:1WZ2"
FT   HELIX           830..851
FT                   /evidence="ECO:0007829|PDB:1WZ2"
FT   STRAND          858..863
FT                   /evidence="ECO:0007829|PDB:1WZ2"
FT   TURN            867..873
FT                   /evidence="ECO:0007829|PDB:1WZ2"
FT   HELIX           874..877
FT                   /evidence="ECO:0007829|PDB:1WZ2"
FT   TURN            878..880
FT                   /evidence="ECO:0007829|PDB:1WZ2"
FT   STRAND          882..884
FT                   /evidence="ECO:0007829|PDB:1WZ2"
FT   HELIX           885..891
FT                   /evidence="ECO:0007829|PDB:1WZ2"
FT   TURN            894..899
FT                   /evidence="ECO:0007829|PDB:1WZ2"
FT   HELIX           900..902
FT                   /evidence="ECO:0007829|PDB:1WZ2"
FT   HELIX           905..910
FT                   /evidence="ECO:0007829|PDB:1WZ2"
FT   HELIX           922..927
FT                   /evidence="ECO:0007829|PDB:1WZ2"
FT   TURN            928..930
FT                   /evidence="ECO:0007829|PDB:1WZ2"
FT   HELIX           931..938
FT                   /evidence="ECO:0007829|PDB:1WZ2"
FT   STRAND          963..966
FT                   /evidence="ECO:0007829|PDB:1WZ2"
SQ   SEQUENCE   967 AA;  113949 MW;  DEFBD287679E9498 CRC64;
     MAELNFKAIE EKWQKRWLEA KIFEPNIRDK PKEKKFYITV AFPYLSGHLH VGHARTYTIP
     DVIARFKRMQ GYNVLFPMAW HITGSPIVGI AERIKNRDPK TIWIYRDVYK VPEEILWTFE
     DPINIVKYFM KAAKETFIRA GFSVDWSREF YTTSLFPPFS KFIEWQFWKL KEKGYIVKGA
     HRVRWDPVVG TPLGDHDLME GEDVPILDYI IIKFELRENG EVIYLPAATL RPETVYGVTN
     MWVNPNATYV KAKVRRKDKE ETWIVSKEAA YKLSFQDREI EVIEEFKGEK LIGKYVRNPV
     SGDEVIILPA EFVDPDNATG VVMSVPAHAP FDHVALEDLK RETEILEKYD IDPRIVENIT
     YISLIKLEGY GDFPAVEEVN KLGIKSQKDK EKLEQATKTI YKAEYHKGIF KVPPYEGKPV
     QEVKEAIAKE MLEKGIAEIM YEFAEKNVIS RFGNRAVIKI IHDQWFIDYG NPEWKEKARK
     ALERMKILPE TRRAQFEAII DWLDKKACAR KIGLGTPLPW DPEWVIESLS DSTIYMAYYT
     ISRHINKLRQ EGKLDPEKLT PEFFDYIFLE EFSEDKEKEL EKKTGIPAEI IHEMKEEFEY
     WYPLDWRCSG KDLIPNHLTF FIFNHVAIFR EEHWPKGIAV NGFGTLEGQK MSKSKGNVLN
     FIDAIEENGA DVVRLYIMSL AEHDSDFDWR RKEVGKLRKQ IERFYELISQ FAEYEVKGNV
     ELKDIDRWML HRLNKAIKET TNALEEFRTR TAVQWAFYSI MNDLRWYLRR TEGRDDEAKR
     YVLRTLADVW VRLMAPFTPH ICEELWEKLG GEGFVSLAKW PEPVEEWWNE TIEAEEEFIR
     SVMEDIKEII EVAKIENAKR AYIYTAEDWK WKVAEVVSEK RDFKSSMEEL MKDSEIRKHG
     KEVAKIVQKL IKERTFDVKR INEEKALREA KEFMEKELGI EIIINPTEDK GGKKKQAMPL
     KPAIFIE
//