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O58698

- SYL_PYRHO

UniProt

O58698 - SYL_PYRHO

Protein

Leucine--tRNA ligase

Gene

leuS

Organism
Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Status
Reviewed - Annotation score: 2 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 101 (01 Oct 2014)
      Sequence version 1 (01 Aug 1998)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei653 – 6531ATPUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. leucine-tRNA ligase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. leucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciPHOR70601:GJWR-959-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Leucine--tRNA ligaseUniRule annotation (EC:6.1.1.4UniRule annotation)
    Alternative name(s):
    Leucyl-tRNA synthetaseUniRule annotation
    Short name:
    LeuRSUniRule annotation
    Gene namesi
    Name:leuSUniRule annotation
    Ordered Locus Names:PH0965
    OrganismiPyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
    Taxonomic identifieri70601 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
    ProteomesiUP000000752: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 967967Leucine--tRNA ligasePRO_0000152140Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi70601.PH0965.

    Structurei

    Secondary structure

    1
    967
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi7 – 1913
    Turni20 – 234
    Helixi27 – 293
    Helixi32 – 343
    Beta strandi35 – 406
    Helixi51 – 7020
    Beta strandi73 – 753
    Helixi86 – 9510
    Helixi99 – 1079
    Helixi113 – 1175
    Helixi118 – 1203
    Helixi122 – 13918
    Helixi146 – 1483
    Helixi157 – 17216
    Beta strandi176 – 18611
    Turni187 – 1904
    Beta strandi195 – 1973
    Beta strandi206 – 21510
    Beta strandi219 – 2213
    Beta strandi224 – 2307
    Helixi232 – 2376
    Beta strandi240 – 2434
    Beta strandi245 – 2473
    Beta strandi249 – 2568
    Beta strandi259 – 2668
    Helixi267 – 2737
    Beta strandi279 – 2857
    Turni287 – 2937
    Beta strandi295 – 2973
    Beta strandi299 – 3013
    Beta strandi304 – 3096
    Beta strandi315 – 3173
    Beta strandi322 – 3243
    Turni326 – 3283
    Helixi330 – 3367
    Helixi380 – 3823
    Helixi394 – 40613
    Helixi413 – 4153
    Beta strandi417 – 4193
    Helixi420 – 43213
    Turni433 – 4353
    Beta strandi436 – 44611
    Beta strandi451 – 4555
    Beta strandi457 – 46711
    Helixi472 – 48312
    Beta strandi485 – 4895
    Helixi490 – 4923
    Helixi493 – 50210
    Beta strandi509 – 5157
    Beta strandi521 – 5266
    Turni528 – 5303
    Beta strandi531 – 5344
    Helixi535 – 55016
    Helixi556 – 5583
    Helixi561 – 5699
    Helixi574 – 58411
    Helixi588 – 60114
    Beta strandi605 – 6106
    Helixi611 – 6133
    Turni614 – 6163
    Helixi617 – 62812
    Helixi631 – 6333
    Beta strandi637 – 6415
    Beta strandi644 – 6463
    Turni653 – 6564
    Helixi661 – 6688
    Helixi670 – 68011
    Beta strandi687 – 6893
    Helixi691 – 71222
    Helixi724 – 74522
    Helixi749 – 7568
    Helixi758 – 77013
    Turni771 – 7733
    Helixi777 – 79418
    Turni795 – 7973
    Helixi799 – 80810
    Helixi815 – 8173
    Helixi830 – 85122
    Beta strandi858 – 8636
    Turni867 – 8737
    Helixi874 – 8774
    Turni878 – 8803
    Beta strandi882 – 8843
    Helixi885 – 8917
    Turni894 – 8996
    Helixi900 – 9023
    Helixi905 – 9106
    Helixi922 – 9276
    Turni928 – 9303
    Helixi931 – 9388
    Beta strandi963 – 9664

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1WKBX-ray2.05A1-810[»]
    1WZ2X-ray3.21A/B1-967[»]
    ProteinModelPortaliO58698.
    SMRiO58698. Positions 4-967.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiO58698.

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi43 – 5311"HIGH" regionAdd
    BLAST
    Motifi650 – 6545"KMSKS" region

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0495.
    HOGENOMiHOG000216620.
    KOiK01869.
    OMAiIDKWLLH.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_00049_A. Leu_tRNA_synth_A.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR020791. Leu-tRNA-lgase_arc.
    IPR004493. Leu-tRNA-synth_Ia_arc/euk.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view]
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view]
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 2 hits.
    TIGRFAMsiTIGR00395. leuS_arch. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O58698-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAELNFKAIE EKWQKRWLEA KIFEPNIRDK PKEKKFYITV AFPYLSGHLH    50
    VGHARTYTIP DVIARFKRMQ GYNVLFPMAW HITGSPIVGI AERIKNRDPK 100
    TIWIYRDVYK VPEEILWTFE DPINIVKYFM KAAKETFIRA GFSVDWSREF 150
    YTTSLFPPFS KFIEWQFWKL KEKGYIVKGA HRVRWDPVVG TPLGDHDLME 200
    GEDVPILDYI IIKFELRENG EVIYLPAATL RPETVYGVTN MWVNPNATYV 250
    KAKVRRKDKE ETWIVSKEAA YKLSFQDREI EVIEEFKGEK LIGKYVRNPV 300
    SGDEVIILPA EFVDPDNATG VVMSVPAHAP FDHVALEDLK RETEILEKYD 350
    IDPRIVENIT YISLIKLEGY GDFPAVEEVN KLGIKSQKDK EKLEQATKTI 400
    YKAEYHKGIF KVPPYEGKPV QEVKEAIAKE MLEKGIAEIM YEFAEKNVIS 450
    RFGNRAVIKI IHDQWFIDYG NPEWKEKARK ALERMKILPE TRRAQFEAII 500
    DWLDKKACAR KIGLGTPLPW DPEWVIESLS DSTIYMAYYT ISRHINKLRQ 550
    EGKLDPEKLT PEFFDYIFLE EFSEDKEKEL EKKTGIPAEI IHEMKEEFEY 600
    WYPLDWRCSG KDLIPNHLTF FIFNHVAIFR EEHWPKGIAV NGFGTLEGQK 650
    MSKSKGNVLN FIDAIEENGA DVVRLYIMSL AEHDSDFDWR RKEVGKLRKQ 700
    IERFYELISQ FAEYEVKGNV ELKDIDRWML HRLNKAIKET TNALEEFRTR 750
    TAVQWAFYSI MNDLRWYLRR TEGRDDEAKR YVLRTLADVW VRLMAPFTPH 800
    ICEELWEKLG GEGFVSLAKW PEPVEEWWNE TIEAEEEFIR SVMEDIKEII 850
    EVAKIENAKR AYIYTAEDWK WKVAEVVSEK RDFKSSMEEL MKDSEIRKHG 900
    KEVAKIVQKL IKERTFDVKR INEEKALREA KEFMEKELGI EIIINPTEDK 950
    GGKKKQAMPL KPAIFIE 967
    Length:967
    Mass (Da):113,949
    Last modified:August 1, 1998 - v1
    Checksum:iDEFBD287679E9498
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000001 Genomic DNA. Translation: BAA30062.1.
    PIRiH71087.
    RefSeqiNP_142884.1. NC_000961.1.

    Genome annotation databases

    EnsemblBacteriaiBAA30062; BAA30062; BAA30062.
    GeneIDi1443290.
    KEGGipho:PH0965.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000001 Genomic DNA. Translation: BAA30062.1 .
    PIRi H71087.
    RefSeqi NP_142884.1. NC_000961.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1WKB X-ray 2.05 A 1-810 [» ]
    1WZ2 X-ray 3.21 A/B 1-967 [» ]
    ProteinModelPortali O58698.
    SMRi O58698. Positions 4-967.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 70601.PH0965.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAA30062 ; BAA30062 ; BAA30062 .
    GeneIDi 1443290.
    KEGGi pho:PH0965.

    Phylogenomic databases

    eggNOGi COG0495.
    HOGENOMi HOG000216620.
    KOi K01869.
    OMAi IDKWLLH.

    Enzyme and pathway databases

    BioCyci PHOR70601:GJWR-959-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei O58698.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_00049_A. Leu_tRNA_synth_A.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR020791. Leu-tRNA-lgase_arc.
    IPR004493. Leu-tRNA-synth_Ia_arc/euk.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view ]
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 2 hits.
    TIGRFAMsi TIGR00395. leuS_arch. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3.
    2. "The crystal structure of leucyl-tRNA synthetase and tRNA(leucine) complex."
      RIKEN structural genomics initiative (RSGI)
      Submitted (SEP-2005) to the PDB data bank
      Cited for: X-RAY CRYSTALLOGRAPHY (3.21 ANGSTROMS).

    Entry informationi

    Entry nameiSYL_PYRHO
    AccessioniPrimary (citable) accession number: O58698
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 30, 2000
    Last sequence update: August 1, 1998
    Last modified: October 1, 2014
    This is version 101 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3