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O58698

- SYL_PYRHO

UniProt

O58698 - SYL_PYRHO

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Protein
Leucine--tRNA ligase
Gene
leuS, PH0965
Organism
Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei653 – 6531ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. aminoacyl-tRNA editing activity Source: InterPro
  3. leucine-tRNA ligase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. leucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciPHOR70601:GJWR-959-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligase (EC:6.1.1.4)
Alternative name(s):
Leucyl-tRNA synthetase
Short name:
LeuRS
Gene namesi
Name:leuS
Ordered Locus Names:PH0965
OrganismiPyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Taxonomic identifieri70601 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
ProteomesiUP000000752: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 967967Leucine--tRNA ligaseUniRule annotation
PRO_0000152140Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi70601.PH0965.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi7 – 1913
Turni20 – 234
Helixi27 – 293
Helixi32 – 343
Beta strandi35 – 406
Helixi51 – 7020
Beta strandi73 – 753
Helixi86 – 9510
Helixi99 – 1079
Helixi113 – 1175
Helixi118 – 1203
Helixi122 – 13918
Helixi146 – 1483
Helixi157 – 17216
Beta strandi176 – 18611
Turni187 – 1904
Beta strandi195 – 1973
Beta strandi206 – 21510
Beta strandi219 – 2213
Beta strandi224 – 2307
Helixi232 – 2376
Beta strandi240 – 2434
Beta strandi245 – 2473
Beta strandi249 – 2568
Beta strandi259 – 2668
Helixi267 – 2737
Beta strandi279 – 2857
Turni287 – 2937
Beta strandi295 – 2973
Beta strandi299 – 3013
Beta strandi304 – 3096
Beta strandi315 – 3173
Beta strandi322 – 3243
Turni326 – 3283
Helixi330 – 3367
Helixi380 – 3823
Helixi394 – 40613
Helixi413 – 4153
Beta strandi417 – 4193
Helixi420 – 43213
Turni433 – 4353
Beta strandi436 – 44611
Beta strandi451 – 4555
Beta strandi457 – 46711
Helixi472 – 48312
Beta strandi485 – 4895
Helixi490 – 4923
Helixi493 – 50210
Beta strandi509 – 5157
Beta strandi521 – 5266
Turni528 – 5303
Beta strandi531 – 5344
Helixi535 – 55016
Helixi556 – 5583
Helixi561 – 5699
Helixi574 – 58411
Helixi588 – 60114
Beta strandi605 – 6106
Helixi611 – 6133
Turni614 – 6163
Helixi617 – 62812
Helixi631 – 6333
Beta strandi637 – 6415
Beta strandi644 – 6463
Turni653 – 6564
Helixi661 – 6688
Helixi670 – 68011
Beta strandi687 – 6893
Helixi691 – 71222
Helixi724 – 74522
Helixi749 – 7568
Helixi758 – 77013
Turni771 – 7733
Helixi777 – 79418
Turni795 – 7973
Helixi799 – 80810
Helixi815 – 8173
Helixi830 – 85122
Beta strandi858 – 8636
Turni867 – 8737
Helixi874 – 8774
Turni878 – 8803
Beta strandi882 – 8843
Helixi885 – 8917
Turni894 – 8996
Helixi900 – 9023
Helixi905 – 9106
Helixi922 – 9276
Turni928 – 9303
Helixi931 – 9388
Beta strandi963 – 9664

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1WKBX-ray2.05A1-810[»]
1WZ2X-ray3.21A/B1-967[»]
ProteinModelPortaliO58698.
SMRiO58698. Positions 4-967.

Miscellaneous databases

EvolutionaryTraceiO58698.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi43 – 5311"HIGH" regionUniRule annotation
Add
BLAST
Motifi650 – 6545"KMSKS" regionUniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000216620.
KOiK01869.
OMAiIDKWLLH.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_A. Leu_tRNA_synth_A.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR020791. Leu-tRNA-lgase_arc.
IPR004493. Leu-tRNA-synth_Ia_arc/euk.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 2 hits.
TIGRFAMsiTIGR00395. leuS_arch. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O58698-1 [UniParc]FASTAAdd to Basket

« Hide

MAELNFKAIE EKWQKRWLEA KIFEPNIRDK PKEKKFYITV AFPYLSGHLH    50
VGHARTYTIP DVIARFKRMQ GYNVLFPMAW HITGSPIVGI AERIKNRDPK 100
TIWIYRDVYK VPEEILWTFE DPINIVKYFM KAAKETFIRA GFSVDWSREF 150
YTTSLFPPFS KFIEWQFWKL KEKGYIVKGA HRVRWDPVVG TPLGDHDLME 200
GEDVPILDYI IIKFELRENG EVIYLPAATL RPETVYGVTN MWVNPNATYV 250
KAKVRRKDKE ETWIVSKEAA YKLSFQDREI EVIEEFKGEK LIGKYVRNPV 300
SGDEVIILPA EFVDPDNATG VVMSVPAHAP FDHVALEDLK RETEILEKYD 350
IDPRIVENIT YISLIKLEGY GDFPAVEEVN KLGIKSQKDK EKLEQATKTI 400
YKAEYHKGIF KVPPYEGKPV QEVKEAIAKE MLEKGIAEIM YEFAEKNVIS 450
RFGNRAVIKI IHDQWFIDYG NPEWKEKARK ALERMKILPE TRRAQFEAII 500
DWLDKKACAR KIGLGTPLPW DPEWVIESLS DSTIYMAYYT ISRHINKLRQ 550
EGKLDPEKLT PEFFDYIFLE EFSEDKEKEL EKKTGIPAEI IHEMKEEFEY 600
WYPLDWRCSG KDLIPNHLTF FIFNHVAIFR EEHWPKGIAV NGFGTLEGQK 650
MSKSKGNVLN FIDAIEENGA DVVRLYIMSL AEHDSDFDWR RKEVGKLRKQ 700
IERFYELISQ FAEYEVKGNV ELKDIDRWML HRLNKAIKET TNALEEFRTR 750
TAVQWAFYSI MNDLRWYLRR TEGRDDEAKR YVLRTLADVW VRLMAPFTPH 800
ICEELWEKLG GEGFVSLAKW PEPVEEWWNE TIEAEEEFIR SVMEDIKEII 850
EVAKIENAKR AYIYTAEDWK WKVAEVVSEK RDFKSSMEEL MKDSEIRKHG 900
KEVAKIVQKL IKERTFDVKR INEEKALREA KEFMEKELGI EIIINPTEDK 950
GGKKKQAMPL KPAIFIE 967
Length:967
Mass (Da):113,949
Last modified:August 1, 1998 - v1
Checksum:iDEFBD287679E9498
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000001 Genomic DNA. Translation: BAA30062.1.
PIRiH71087.
RefSeqiNP_142884.1. NC_000961.1.

Genome annotation databases

EnsemblBacteriaiBAA30062; BAA30062; BAA30062.
GeneIDi1443290.
KEGGipho:PH0965.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000001 Genomic DNA. Translation: BAA30062.1 .
PIRi H71087.
RefSeqi NP_142884.1. NC_000961.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1WKB X-ray 2.05 A 1-810 [» ]
1WZ2 X-ray 3.21 A/B 1-967 [» ]
ProteinModelPortali O58698.
SMRi O58698. Positions 4-967.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 70601.PH0965.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAA30062 ; BAA30062 ; BAA30062 .
GeneIDi 1443290.
KEGGi pho:PH0965.

Phylogenomic databases

eggNOGi COG0495.
HOGENOMi HOG000216620.
KOi K01869.
OMAi IDKWLLH.

Enzyme and pathway databases

BioCyci PHOR70601:GJWR-959-MONOMER.

Miscellaneous databases

EvolutionaryTracei O58698.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_00049_A. Leu_tRNA_synth_A.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR020791. Leu-tRNA-lgase_arc.
IPR004493. Leu-tRNA-synth_Ia_arc/euk.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view ]
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 2 hits.
TIGRFAMsi TIGR00395. leuS_arch. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3.
  2. "The crystal structure of leucyl-tRNA synthetase and tRNA(leucine) complex."
    RIKEN structural genomics initiative (RSGI)
    Submitted (SEP-2005) to the PDB data bank
    Cited for: X-RAY CRYSTALLOGRAPHY (3.21 ANGSTROMS).

Entry informationi

Entry nameiSYL_PYRHO
AccessioniPrimary (citable) accession number: O58698
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: August 1, 1998
Last modified: May 14, 2014
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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