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Protein

Leucine--tRNA ligase

Gene

leuS

Organism
Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei653ATPUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciPHOR70601:G1G39-939-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligaseUniRule annotation (EC:6.1.1.4UniRule annotation)
Alternative name(s):
Leucyl-tRNA synthetaseUniRule annotation
Short name:
LeuRSUniRule annotation
Gene namesi
Name:leuSUniRule annotation
Ordered Locus Names:PH0965
OrganismiPyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Taxonomic identifieri70601 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
Proteomesi
  • UP000000752 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001521401 – 967Leucine--tRNA ligaseAdd BLAST967

Interactioni

Protein-protein interaction databases

STRINGi70601.PH0965

Structurei

Secondary structure

1967
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi7 – 19Combined sources13
Turni20 – 23Combined sources4
Helixi27 – 29Combined sources3
Helixi32 – 34Combined sources3
Beta strandi35 – 40Combined sources6
Helixi51 – 70Combined sources20
Beta strandi73 – 75Combined sources3
Helixi86 – 95Combined sources10
Helixi99 – 107Combined sources9
Helixi113 – 117Combined sources5
Helixi118 – 120Combined sources3
Helixi122 – 139Combined sources18
Helixi146 – 148Combined sources3
Helixi157 – 172Combined sources16
Beta strandi176 – 186Combined sources11
Turni187 – 190Combined sources4
Beta strandi195 – 197Combined sources3
Beta strandi206 – 215Combined sources10
Beta strandi219 – 221Combined sources3
Beta strandi224 – 230Combined sources7
Helixi232 – 237Combined sources6
Beta strandi240 – 243Combined sources4
Beta strandi245 – 247Combined sources3
Beta strandi249 – 256Combined sources8
Beta strandi259 – 266Combined sources8
Helixi267 – 273Combined sources7
Beta strandi279 – 285Combined sources7
Turni287 – 293Combined sources7
Beta strandi295 – 297Combined sources3
Beta strandi299 – 301Combined sources3
Beta strandi304 – 309Combined sources6
Beta strandi315 – 317Combined sources3
Beta strandi322 – 324Combined sources3
Turni326 – 328Combined sources3
Helixi330 – 336Combined sources7
Helixi380 – 382Combined sources3
Helixi394 – 406Combined sources13
Helixi413 – 415Combined sources3
Beta strandi417 – 419Combined sources3
Helixi420 – 432Combined sources13
Turni433 – 435Combined sources3
Beta strandi436 – 446Combined sources11
Beta strandi451 – 455Combined sources5
Beta strandi457 – 467Combined sources11
Helixi472 – 483Combined sources12
Beta strandi485 – 489Combined sources5
Helixi490 – 492Combined sources3
Helixi493 – 502Combined sources10
Beta strandi509 – 515Combined sources7
Beta strandi521 – 526Combined sources6
Turni528 – 530Combined sources3
Beta strandi531 – 534Combined sources4
Helixi535 – 550Combined sources16
Helixi556 – 558Combined sources3
Helixi561 – 569Combined sources9
Helixi574 – 584Combined sources11
Helixi588 – 601Combined sources14
Beta strandi605 – 610Combined sources6
Helixi611 – 613Combined sources3
Turni614 – 616Combined sources3
Helixi617 – 628Combined sources12
Helixi631 – 633Combined sources3
Beta strandi637 – 641Combined sources5
Beta strandi644 – 646Combined sources3
Turni653 – 656Combined sources4
Helixi661 – 668Combined sources8
Helixi670 – 680Combined sources11
Beta strandi687 – 689Combined sources3
Helixi691 – 712Combined sources22
Helixi724 – 745Combined sources22
Helixi749 – 756Combined sources8
Helixi758 – 770Combined sources13
Turni771 – 773Combined sources3
Helixi777 – 794Combined sources18
Turni795 – 797Combined sources3
Helixi799 – 808Combined sources10
Helixi815 – 817Combined sources3
Helixi830 – 851Combined sources22
Beta strandi858 – 863Combined sources6
Turni867 – 873Combined sources7
Helixi874 – 877Combined sources4
Turni878 – 880Combined sources3
Beta strandi882 – 884Combined sources3
Helixi885 – 891Combined sources7
Turni894 – 899Combined sources6
Helixi900 – 902Combined sources3
Helixi905 – 910Combined sources6
Helixi922 – 927Combined sources6
Turni928 – 930Combined sources3
Helixi931 – 938Combined sources8
Beta strandi963 – 966Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1WKBX-ray2.05A1-810[»]
1WZ2X-ray3.21A/B1-967[»]
ProteinModelPortaliO58698
SMRiO58698
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO58698

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi43 – 53"HIGH" regionAdd BLAST11
Motifi650 – 654"KMSKS" region5

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiarCOG00809 Archaea
COG0495 LUCA
HOGENOMiHOG000216620
KOiK01869
OMAiHVTGTPI
OrthoDBiPOG093Z006D

Family and domain databases

Gene3Di3.40.50.620, 2 hits
3.90.740.10, 1 hit
HAMAPiMF_00049_A Leu_tRNA_synth_A, 1 hit
InterProiView protein in InterPro
IPR001412 aa-tRNA-synth_I_CS
IPR002300 aa-tRNA-synth_Ia
IPR020791 Leu-tRNA-lgase_arc
IPR004493 Leu-tRNA-synth_Ia_arc/euk
IPR013155 M/V/L/I-tRNA-synth_anticd-bd
IPR014729 Rossmann-like_a/b/a_fold
IPR009080 tRNAsynth_Ia_anticodon-bd
IPR009008 Val/Leu/Ile-tRNA-synth_edit
PANTHERiPTHR11946:SF51 PTHR11946:SF51, 1 hit
PfamiView protein in Pfam
PF08264 Anticodon_1, 1 hit
PF00133 tRNA-synt_1, 1 hit
SUPFAMiSSF47323 SSF47323, 1 hit
SSF50677 SSF50677, 2 hits
TIGRFAMsiTIGR00395 leuS_arch, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit

Sequencei

Sequence statusi: Complete.

O58698-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAELNFKAIE EKWQKRWLEA KIFEPNIRDK PKEKKFYITV AFPYLSGHLH
60 70 80 90 100
VGHARTYTIP DVIARFKRMQ GYNVLFPMAW HITGSPIVGI AERIKNRDPK
110 120 130 140 150
TIWIYRDVYK VPEEILWTFE DPINIVKYFM KAAKETFIRA GFSVDWSREF
160 170 180 190 200
YTTSLFPPFS KFIEWQFWKL KEKGYIVKGA HRVRWDPVVG TPLGDHDLME
210 220 230 240 250
GEDVPILDYI IIKFELRENG EVIYLPAATL RPETVYGVTN MWVNPNATYV
260 270 280 290 300
KAKVRRKDKE ETWIVSKEAA YKLSFQDREI EVIEEFKGEK LIGKYVRNPV
310 320 330 340 350
SGDEVIILPA EFVDPDNATG VVMSVPAHAP FDHVALEDLK RETEILEKYD
360 370 380 390 400
IDPRIVENIT YISLIKLEGY GDFPAVEEVN KLGIKSQKDK EKLEQATKTI
410 420 430 440 450
YKAEYHKGIF KVPPYEGKPV QEVKEAIAKE MLEKGIAEIM YEFAEKNVIS
460 470 480 490 500
RFGNRAVIKI IHDQWFIDYG NPEWKEKARK ALERMKILPE TRRAQFEAII
510 520 530 540 550
DWLDKKACAR KIGLGTPLPW DPEWVIESLS DSTIYMAYYT ISRHINKLRQ
560 570 580 590 600
EGKLDPEKLT PEFFDYIFLE EFSEDKEKEL EKKTGIPAEI IHEMKEEFEY
610 620 630 640 650
WYPLDWRCSG KDLIPNHLTF FIFNHVAIFR EEHWPKGIAV NGFGTLEGQK
660 670 680 690 700
MSKSKGNVLN FIDAIEENGA DVVRLYIMSL AEHDSDFDWR RKEVGKLRKQ
710 720 730 740 750
IERFYELISQ FAEYEVKGNV ELKDIDRWML HRLNKAIKET TNALEEFRTR
760 770 780 790 800
TAVQWAFYSI MNDLRWYLRR TEGRDDEAKR YVLRTLADVW VRLMAPFTPH
810 820 830 840 850
ICEELWEKLG GEGFVSLAKW PEPVEEWWNE TIEAEEEFIR SVMEDIKEII
860 870 880 890 900
EVAKIENAKR AYIYTAEDWK WKVAEVVSEK RDFKSSMEEL MKDSEIRKHG
910 920 930 940 950
KEVAKIVQKL IKERTFDVKR INEEKALREA KEFMEKELGI EIIINPTEDK
960
GGKKKQAMPL KPAIFIE
Length:967
Mass (Da):113,949
Last modified:August 1, 1998 - v1
Checksum:iDEFBD287679E9498
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000001 Genomic DNA Translation: BAA30062.1
PIRiH71087
RefSeqiWP_010885055.1, NC_000961.1

Genome annotation databases

EnsemblBacteriaiBAA30062; BAA30062; BAA30062
GeneIDi1443290
KEGGipho:PH0965

Similar proteinsi

Entry informationi

Entry nameiSYL_PYRHO
AccessioniPrimary (citable) accession number: O58698
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: August 1, 1998
Last modified: April 25, 2018
This is version 124 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

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