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O58698 (SYL_PYRHO) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 99. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:PH0965
OrganismPyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3) [Complete proteome] [HAMAP]
Taxonomic identifier70601 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus

Protein attributes

Sequence length967 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical term3D-structure
Complete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 967967Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_0000152140

Regions

Motif43 – 5311"HIGH" region HAMAP-Rule MF_00049
Motif650 – 6545"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site6531ATP By similarity

Secondary structure

................................................................................................................................................................ 967
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
O58698 [UniParc].

Last modified August 1, 1998. Version 1.
Checksum: DEFBD287679E9498

FASTA967113,949
        10         20         30         40         50         60 
MAELNFKAIE EKWQKRWLEA KIFEPNIRDK PKEKKFYITV AFPYLSGHLH VGHARTYTIP 

        70         80         90        100        110        120 
DVIARFKRMQ GYNVLFPMAW HITGSPIVGI AERIKNRDPK TIWIYRDVYK VPEEILWTFE 

       130        140        150        160        170        180 
DPINIVKYFM KAAKETFIRA GFSVDWSREF YTTSLFPPFS KFIEWQFWKL KEKGYIVKGA 

       190        200        210        220        230        240 
HRVRWDPVVG TPLGDHDLME GEDVPILDYI IIKFELRENG EVIYLPAATL RPETVYGVTN 

       250        260        270        280        290        300 
MWVNPNATYV KAKVRRKDKE ETWIVSKEAA YKLSFQDREI EVIEEFKGEK LIGKYVRNPV 

       310        320        330        340        350        360 
SGDEVIILPA EFVDPDNATG VVMSVPAHAP FDHVALEDLK RETEILEKYD IDPRIVENIT 

       370        380        390        400        410        420 
YISLIKLEGY GDFPAVEEVN KLGIKSQKDK EKLEQATKTI YKAEYHKGIF KVPPYEGKPV 

       430        440        450        460        470        480 
QEVKEAIAKE MLEKGIAEIM YEFAEKNVIS RFGNRAVIKI IHDQWFIDYG NPEWKEKARK 

       490        500        510        520        530        540 
ALERMKILPE TRRAQFEAII DWLDKKACAR KIGLGTPLPW DPEWVIESLS DSTIYMAYYT 

       550        560        570        580        590        600 
ISRHINKLRQ EGKLDPEKLT PEFFDYIFLE EFSEDKEKEL EKKTGIPAEI IHEMKEEFEY 

       610        620        630        640        650        660 
WYPLDWRCSG KDLIPNHLTF FIFNHVAIFR EEHWPKGIAV NGFGTLEGQK MSKSKGNVLN 

       670        680        690        700        710        720 
FIDAIEENGA DVVRLYIMSL AEHDSDFDWR RKEVGKLRKQ IERFYELISQ FAEYEVKGNV 

       730        740        750        760        770        780 
ELKDIDRWML HRLNKAIKET TNALEEFRTR TAVQWAFYSI MNDLRWYLRR TEGRDDEAKR 

       790        800        810        820        830        840 
YVLRTLADVW VRLMAPFTPH ICEELWEKLG GEGFVSLAKW PEPVEEWWNE TIEAEEEFIR 

       850        860        870        880        890        900 
SVMEDIKEII EVAKIENAKR AYIYTAEDWK WKVAEVVSEK RDFKSSMEEL MKDSEIRKHG 

       910        920        930        940        950        960 
KEVAKIVQKL IKERTFDVKR INEEKALREA KEFMEKELGI EIIINPTEDK GGKKKQAMPL 


KPAIFIE 

« Hide

References

« Hide 'large scale' references
[1]"Complete sequence and gene organization of the genome of a hyper-thermophilic archaebacterium, Pyrococcus horikoshii OT3."
Kawarabayasi Y., Sawada M., Horikawa H., Haikawa Y., Hino Y., Yamamoto S., Sekine M., Baba S., Kosugi H., Hosoyama A., Nagai Y., Sakai M., Ogura K., Otsuka R., Nakazawa H., Takamiya M., Ohfuku Y., Funahashi T. expand/collapse author list , Tanaka T., Kudoh Y., Yamazaki J., Kushida N., Oguchi A., Aoki K., Yoshizawa T., Nakamura Y., Robb F.T., Horikoshi K., Masuchi Y., Shizuya H., Kikuchi H.
DNA Res. 5:55-76(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3.
[2]"The crystal structure of leucyl-tRNA synthetase and tRNA(leucine) complex."
RIKEN structural genomics initiative (RSGI)
Submitted (SEP-2005) to the PDB data bank
Cited for: X-RAY CRYSTALLOGRAPHY (3.21 ANGSTROMS).
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BA000001 Genomic DNA. Translation: BAA30062.1.
PIRH71087.
RefSeqNP_142884.1. NC_000961.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1WKBX-ray2.05A1-810[»]
1WZ2X-ray3.21A/B1-967[»]
ProteinModelPortalO58698.
SMRO58698. Positions 4-967.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING70601.PH0965.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAA30062; BAA30062; BAA30062.
GeneID1443290.
KEGGpho:PH0965.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000216620.
KOK01869.
OMAIMNDLRW.
ProtClustDBPRK12300.

Enzyme and pathway databases

BioCycPHOR70601:GJWR-959-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_A. Leu_tRNA_synth_A.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR020791. Leu-tRNA-lgase_arc.
IPR004493. Leu-tRNA-synth_Ia_arc/euk.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 2 hits.
TIGRFAMsTIGR00395. leuS_arch. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceO58698.

Entry information

Entry nameSYL_PYRHO
AccessionPrimary (citable) accession number: O58698
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: August 1, 1998
Last modified: April 16, 2014
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries