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O58698

- SYL_PYRHO

UniProt

O58698 - SYL_PYRHO

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Protein

Leucine--tRNA ligase

Gene

leuS

Organism
Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Status
Reviewed - Annotation score: 2 out of 5- Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei653 – 6531ATPUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. leucine-tRNA ligase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. leucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciPHOR70601:GJWR-959-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligaseUniRule annotation (EC:6.1.1.4UniRule annotation)
Alternative name(s):
Leucyl-tRNA synthetaseUniRule annotation
Short name:
LeuRSUniRule annotation
Gene namesi
Name:leuSUniRule annotation
Ordered Locus Names:PH0965
OrganismiPyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Taxonomic identifieri70601 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
ProteomesiUP000000752: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 967967Leucine--tRNA ligasePRO_0000152140Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi70601.PH0965.

Structurei

Secondary structure

1
967
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi7 – 1913Combined sources
Turni20 – 234Combined sources
Helixi27 – 293Combined sources
Helixi32 – 343Combined sources
Beta strandi35 – 406Combined sources
Helixi51 – 7020Combined sources
Beta strandi73 – 753Combined sources
Helixi86 – 9510Combined sources
Helixi99 – 1079Combined sources
Helixi113 – 1175Combined sources
Helixi118 – 1203Combined sources
Helixi122 – 13918Combined sources
Helixi146 – 1483Combined sources
Helixi157 – 17216Combined sources
Beta strandi176 – 18611Combined sources
Turni187 – 1904Combined sources
Beta strandi195 – 1973Combined sources
Beta strandi206 – 21510Combined sources
Beta strandi219 – 2213Combined sources
Beta strandi224 – 2307Combined sources
Helixi232 – 2376Combined sources
Beta strandi240 – 2434Combined sources
Beta strandi245 – 2473Combined sources
Beta strandi249 – 2568Combined sources
Beta strandi259 – 2668Combined sources
Helixi267 – 2737Combined sources
Beta strandi279 – 2857Combined sources
Turni287 – 2937Combined sources
Beta strandi295 – 2973Combined sources
Beta strandi299 – 3013Combined sources
Beta strandi304 – 3096Combined sources
Beta strandi315 – 3173Combined sources
Beta strandi322 – 3243Combined sources
Turni326 – 3283Combined sources
Helixi330 – 3367Combined sources
Helixi380 – 3823Combined sources
Helixi394 – 40613Combined sources
Helixi413 – 4153Combined sources
Beta strandi417 – 4193Combined sources
Helixi420 – 43213Combined sources
Turni433 – 4353Combined sources
Beta strandi436 – 44611Combined sources
Beta strandi451 – 4555Combined sources
Beta strandi457 – 46711Combined sources
Helixi472 – 48312Combined sources
Beta strandi485 – 4895Combined sources
Helixi490 – 4923Combined sources
Helixi493 – 50210Combined sources
Beta strandi509 – 5157Combined sources
Beta strandi521 – 5266Combined sources
Turni528 – 5303Combined sources
Beta strandi531 – 5344Combined sources
Helixi535 – 55016Combined sources
Helixi556 – 5583Combined sources
Helixi561 – 5699Combined sources
Helixi574 – 58411Combined sources
Helixi588 – 60114Combined sources
Beta strandi605 – 6106Combined sources
Helixi611 – 6133Combined sources
Turni614 – 6163Combined sources
Helixi617 – 62812Combined sources
Helixi631 – 6333Combined sources
Beta strandi637 – 6415Combined sources
Beta strandi644 – 6463Combined sources
Turni653 – 6564Combined sources
Helixi661 – 6688Combined sources
Helixi670 – 68011Combined sources
Beta strandi687 – 6893Combined sources
Helixi691 – 71222Combined sources
Helixi724 – 74522Combined sources
Helixi749 – 7568Combined sources
Helixi758 – 77013Combined sources
Turni771 – 7733Combined sources
Helixi777 – 79418Combined sources
Turni795 – 7973Combined sources
Helixi799 – 80810Combined sources
Helixi815 – 8173Combined sources
Helixi830 – 85122Combined sources
Beta strandi858 – 8636Combined sources
Turni867 – 8737Combined sources
Helixi874 – 8774Combined sources
Turni878 – 8803Combined sources
Beta strandi882 – 8843Combined sources
Helixi885 – 8917Combined sources
Turni894 – 8996Combined sources
Helixi900 – 9023Combined sources
Helixi905 – 9106Combined sources
Helixi922 – 9276Combined sources
Turni928 – 9303Combined sources
Helixi931 – 9388Combined sources
Beta strandi963 – 9664Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1WKBX-ray2.05A1-810[»]
1WZ2X-ray3.21A/B1-967[»]
ProteinModelPortaliO58698.
SMRiO58698. Positions 4-967.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO58698.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi43 – 5311"HIGH" regionAdd
BLAST
Motifi650 – 6545"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000216620.
KOiK01869.
OMAiIDKWLLH.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_A. Leu_tRNA_synth_A.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR020791. Leu-tRNA-lgase_arc.
IPR004493. Leu-tRNA-synth_Ia_arc/euk.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 2 hits.
TIGRFAMsiTIGR00395. leuS_arch. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O58698-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAELNFKAIE EKWQKRWLEA KIFEPNIRDK PKEKKFYITV AFPYLSGHLH
60 70 80 90 100
VGHARTYTIP DVIARFKRMQ GYNVLFPMAW HITGSPIVGI AERIKNRDPK
110 120 130 140 150
TIWIYRDVYK VPEEILWTFE DPINIVKYFM KAAKETFIRA GFSVDWSREF
160 170 180 190 200
YTTSLFPPFS KFIEWQFWKL KEKGYIVKGA HRVRWDPVVG TPLGDHDLME
210 220 230 240 250
GEDVPILDYI IIKFELRENG EVIYLPAATL RPETVYGVTN MWVNPNATYV
260 270 280 290 300
KAKVRRKDKE ETWIVSKEAA YKLSFQDREI EVIEEFKGEK LIGKYVRNPV
310 320 330 340 350
SGDEVIILPA EFVDPDNATG VVMSVPAHAP FDHVALEDLK RETEILEKYD
360 370 380 390 400
IDPRIVENIT YISLIKLEGY GDFPAVEEVN KLGIKSQKDK EKLEQATKTI
410 420 430 440 450
YKAEYHKGIF KVPPYEGKPV QEVKEAIAKE MLEKGIAEIM YEFAEKNVIS
460 470 480 490 500
RFGNRAVIKI IHDQWFIDYG NPEWKEKARK ALERMKILPE TRRAQFEAII
510 520 530 540 550
DWLDKKACAR KIGLGTPLPW DPEWVIESLS DSTIYMAYYT ISRHINKLRQ
560 570 580 590 600
EGKLDPEKLT PEFFDYIFLE EFSEDKEKEL EKKTGIPAEI IHEMKEEFEY
610 620 630 640 650
WYPLDWRCSG KDLIPNHLTF FIFNHVAIFR EEHWPKGIAV NGFGTLEGQK
660 670 680 690 700
MSKSKGNVLN FIDAIEENGA DVVRLYIMSL AEHDSDFDWR RKEVGKLRKQ
710 720 730 740 750
IERFYELISQ FAEYEVKGNV ELKDIDRWML HRLNKAIKET TNALEEFRTR
760 770 780 790 800
TAVQWAFYSI MNDLRWYLRR TEGRDDEAKR YVLRTLADVW VRLMAPFTPH
810 820 830 840 850
ICEELWEKLG GEGFVSLAKW PEPVEEWWNE TIEAEEEFIR SVMEDIKEII
860 870 880 890 900
EVAKIENAKR AYIYTAEDWK WKVAEVVSEK RDFKSSMEEL MKDSEIRKHG
910 920 930 940 950
KEVAKIVQKL IKERTFDVKR INEEKALREA KEFMEKELGI EIIINPTEDK
960
GGKKKQAMPL KPAIFIE
Length:967
Mass (Da):113,949
Last modified:August 1, 1998 - v1
Checksum:iDEFBD287679E9498
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000001 Genomic DNA. Translation: BAA30062.1.
PIRiH71087.
RefSeqiNP_142884.1. NC_000961.1.
WP_010885055.1. NC_000961.1.

Genome annotation databases

EnsemblBacteriaiBAA30062; BAA30062; BAA30062.
GeneIDi1443290.
KEGGipho:PH0965.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000001 Genomic DNA. Translation: BAA30062.1 .
PIRi H71087.
RefSeqi NP_142884.1. NC_000961.1.
WP_010885055.1. NC_000961.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1WKB X-ray 2.05 A 1-810 [» ]
1WZ2 X-ray 3.21 A/B 1-967 [» ]
ProteinModelPortali O58698.
SMRi O58698. Positions 4-967.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 70601.PH0965.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAA30062 ; BAA30062 ; BAA30062 .
GeneIDi 1443290.
KEGGi pho:PH0965.

Phylogenomic databases

eggNOGi COG0495.
HOGENOMi HOG000216620.
KOi K01869.
OMAi IDKWLLH.

Enzyme and pathway databases

BioCyci PHOR70601:GJWR-959-MONOMER.

Miscellaneous databases

EvolutionaryTracei O58698.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_00049_A. Leu_tRNA_synth_A.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR020791. Leu-tRNA-lgase_arc.
IPR004493. Leu-tRNA-synth_Ia_arc/euk.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view ]
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 2 hits.
TIGRFAMsi TIGR00395. leuS_arch. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3.
  2. "The crystal structure of leucyl-tRNA synthetase and tRNA(leucine) complex."
    RIKEN structural genomics initiative (RSGI)
    Submitted (SEP-2005) to the PDB data bank
    Cited for: X-RAY CRYSTALLOGRAPHY (3.21 ANGSTROMS).

Entry informationi

Entry nameiSYL_PYRHO
AccessioniPrimary (citable) accession number: O58698
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: August 1, 1998
Last modified: November 26, 2014
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3