Reviewed, UniProtKB/Swiss-Prot O58689 (MPGS_PYRHO)

Names and origin

Protein names

Recommended name:
    Mannosyl-3-phosphoglycerate synthase
      Short name=MPG synthase
      Short name=MPGS
    EC=2.4.1.217

Gene names

Name:	mngA
Ordered Locus Names:	PH0927

Organism

Pyrococcus horikoshii [Complete proteome] [HAMAP]

Taxonomic identifier

53953 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Thermococci › Thermococcales › Thermococcaceae › Pyrococcus

Protein attributes

Sequence length	394 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Transfers a mannosyl group from GDP-mannose to phosphoglycerate to form mannosyl-3-phosphoglycerate (MPG). The enzyme is absolutely specific for GDP-mannose and 3-phosphoglycerate, and transfers the mannosyl group with retention of configuration.
Catalytic activity	GDP-mannose + 3-phospho-D-glycerate = GDP + 2-(alpha-D-mannosyl)-3-phosphoglycerate.
Cofactor	Magnesium.
Pathway	Carbohydrate biosynthesis; mannosylglycerate biosynthesis; 2(alpha-D-mannosyl)-D-glycerate from GDP-D-mannose (MPG route): step 1/2.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the glycosyltransferase 2 family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Magnesium
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	mannosylglycerate biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW mannosyl-3-phosphoglycerate synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 394	394	Mannosyl-3-phosphoglycerate synthase		PRO_0000059287

Sequences

	Sequence		Length	Mass (Da)
	O58689-1 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: 6C2FB4141D2CC807	FASTA	394	45,210
	10 20 30 40 50 60 MLLEAPVYKE IFGAVTIHEV QKVIKMDTET EEVPIYTISN IPREKIYDLL GKMAVIVPMK 70 80 90 100 110 120 NEKLHLVDGV LKAIPHKCPI IIVSNSKREG PNRYKLEVDL IRHFYNLTHS KIIMIHQKDP 130 140 150 160 170 180 GLAKAFKEVG YTDILDENGM IRSGKGEGML VGLLLAKAIG AEYVGFVDAD NYIPGAVNEY 190 200 210 220 230 240 VKDYAAGFLM SESEYTMVRL HWRHKPKVTK GTLYFKKWGR VSEITNHYLN LLVSEHTAFE 250 260 270 280 290 300 TTIMVTGNAG EHAMTMKLAE ILPFSTGYSI EPYEIVYILE RFGKWENVEE FKDVFDQGIE 310 320 330 340 350 360 IFQIETLNPH FHEDKGKEHV KEMLLLSLAT IYHSKLATDN LRKRILKDLR DHGILGENEE 370 380 390 PPKPLVMRPI KEIPIKEWMD IVEGNSETLL RFEL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete sequence and gene organization of the genome of a hyper-thermophilic archaebacterium, Pyrococcus horikoshii OT3." Kawarabayasi Y., Sawada M., Horikawa H., Haikawa Y., Hino Y., Yamamoto S., Sekine M., Baba S., Kosugi H., Hosoyama A., Nagai Y., Sakai M., Ogura K., Otsuka R., Nakazawa H., Takamiya M., Ohfuku Y., Funahashi T. , Tanaka T., Kudoh Y., Yamazaki J., Kushida N., Oguchi A., Aoki K., Yoshizawa T., Nakamura Y., Robb F.T., Horikoshi K., Masuchi Y., Shizuya H., Kikuchi H. DNA Res. 5:55-76(1998) [PubMed: 9679194] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: OT3.
[2]	"Pathway for the synthesis of mannosylglycerate in the hyperthermophilic archaeon Pyrococcus horikoshii. Biochemical and genetic characterization of key enzymes." Empadinhas N., Marugg J.D., Borges N., Santos H., da Costa M.S. J. Biol. Chem. 276:43580-43588(2001) [PubMed: 11562374] [Abstract] Cited for: CHARACTERIZATION.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000001 Genomic DNA. Translation: BAA30023.1.
PIR	A71083.
RefSeq	NP_142850.1.
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	GT55. Glycosyltransferase Family 55.
Genome annotation databases
GeneID	1443252.
GenomeReviews	Gene locus PH0927 in contig BA000001_GR.
KEGG	pho:PH0927.
NMPDR	fig|70601.1.peg.905.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	O58689.
OMA	O58689. EIFQIET.
Enzyme and pathway databases
BioCyc	MetaCyc:MON-13380.
BRENDA	2.4.1.217. 74679.
Family and domain databases
InterPro	IPR012812. Osmo_MPG_synth. [Graphical view]
Pfam	PF09488. Osmo_MPGsynth. 1 hit. [Graphical view]
TIGRFAMs	TIGR02460. osmo_MPGsynth. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MPGS_PYRHO

Accession

Primary (citable) accession number: O58689

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 12, 2003
Last sequence update:	August 1, 1998
Last modified:	June 16, 2009
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)