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Protein

Mannosyl-3-phosphoglycerate synthase

Gene

mngA

Organism
Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Transfers a mannosyl group from GDP-mannose to phosphoglycerate to form mannosyl-3-phosphoglycerate (MPG). The enzyme is absolutely specific for GDP-mannose and 3-phosphoglycerate, and transfers the mannosyl group with retention of configuration.

Catalytic activityi

GDP-mannose + 3-phospho-D-glycerate = GDP + 2-(alpha-D-mannosyl)-3-phosphoglycerate.

Cofactori

Pathwayi: 2-(alpha-D-mannosyl)-D-glycerate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes 2-(alpha-D-mannosyl)-D-glycerate from GDP-alpha-D-mannose (MPG route).
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Mannosyl-3-phosphoglycerate synthase (mngA)
  2. Mannosyl-3-phosphoglycerate phosphatase (mngB)
This subpathway is part of the pathway 2-(alpha-D-mannosyl)-D-glycerate biosynthesis, which is itself part of Carbohydrate biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 2-(alpha-D-mannosyl)-D-glycerate from GDP-alpha-D-mannose (MPG route), the pathway 2-(alpha-D-mannosyl)-D-glycerate biosynthesis and in Carbohydrate biosynthesis.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Ligandi

Magnesium

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-13380.
PHOR70601:GJWR-920-MONOMER.
SABIO-RKO58689.
UniPathwayiUPA00130; UER00192.

Protein family/group databases

CAZyiGT55. Glycosyltransferase Family 55.

Names & Taxonomyi

Protein namesi
Recommended name:
Mannosyl-3-phosphoglycerate synthase (EC:2.4.1.217)
Short name:
MPG synthase
Short name:
MPGS
Gene namesi
Name:mngA
Ordered Locus Names:PH0927
OrganismiPyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Taxonomic identifieri70601 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
Proteomesi
  • UP000000752 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 394394Mannosyl-3-phosphoglycerate synthasePRO_0000059287Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi70601.PH0927.

Structurei

Secondary structure

1
394
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi2 – 43Combined sources
Beta strandi9 – 124Combined sources
Beta strandi15 – 184Combined sources
Beta strandi21 – 266Combined sources
Beta strandi38 – 403Combined sources
Helixi43 – 508Combined sources
Beta strandi53 – 619Combined sources
Helixi64 – 7310Combined sources
Beta strandi80 – 845Combined sources
Beta strandi89 – 913Combined sources
Helixi93 – 10816Combined sources
Beta strandi112 – 1165Combined sources
Helixi120 – 1289Combined sources
Beta strandi139 – 1413Combined sources
Helixi145 – 15814Combined sources
Beta strandi162 – 1665Combined sources
Helixi174 – 19017Combined sources
Beta strandi193 – 2019Combined sources
Helixi221 – 23717Combined sources
Beta strandi250 – 2556Combined sources
Helixi256 – 2594Combined sources
Beta strandi264 – 2663Combined sources
Helixi267 – 2693Combined sources
Helixi270 – 28213Combined sources
Beta strandi283 – 2853Combined sources
Helixi289 – 2913Combined sources
Helixi292 – 2976Combined sources
Beta strandi299 – 3057Combined sources
Helixi317 – 33216Combined sources
Helixi339 – 35113Combined sources
Beta strandi370 – 3734Combined sources
Helixi375 – 38511Combined sources
Beta strandi390 – 3923Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2ZU7X-ray2.50A/B1-394[»]
2ZU8X-ray2.40A/B1-394[»]
2ZU9X-ray2.00A/B1-394[»]
ProteinModelPortaliO58689.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO58689.

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyltransferase 2 family.Curated

Phylogenomic databases

eggNOGiarCOG04158. Archaea.
ENOG410ZA90. LUCA.
HOGENOMiHOG000246027.
KOiK05947.
OMAiFIDADNY.

Family and domain databases

InterProiIPR029044. Nucleotide-diphossugar_trans.
IPR012812. Osmo_MPG_synth.
[Graphical view]
PfamiPF09488. Osmo_MPGsynth. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 1 hit.
TIGRFAMsiTIGR02460. osmo_MPGsynth. 1 hit.

Sequencei

Sequence statusi: Complete.

O58689-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLLEAPVYKE IFGAVTIHEV QKVIKMDTET EEVPIYTISN IPREKIYDLL
60 70 80 90 100
GKMAVIVPMK NEKLHLVDGV LKAIPHKCPI IIVSNSKREG PNRYKLEVDL
110 120 130 140 150
IRHFYNLTHS KIIMIHQKDP GLAKAFKEVG YTDILDENGM IRSGKGEGML
160 170 180 190 200
VGLLLAKAIG AEYVGFVDAD NYIPGAVNEY VKDYAAGFLM SESEYTMVRL
210 220 230 240 250
HWRHKPKVTK GTLYFKKWGR VSEITNHYLN LLVSEHTAFE TTIMVTGNAG
260 270 280 290 300
EHAMTMKLAE ILPFSTGYSI EPYEIVYILE RFGKWENVEE FKDVFDQGIE
310 320 330 340 350
IFQIETLNPH FHEDKGKEHV KEMLLLSLAT IYHSKLATDN LRKRILKDLR
360 370 380 390
DHGILGENEE PPKPLVMRPI KEIPIKEWMD IVEGNSETLL RFEL
Length:394
Mass (Da):45,210
Last modified:August 1, 1998 - v1
Checksum:i6C2FB4141D2CC807
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000001 Genomic DNA. Translation: BAA30023.1.
PIRiA71083.
RefSeqiWP_010885017.1. NC_000961.1.

Genome annotation databases

EnsemblBacteriaiBAA30023; BAA30023; BAA30023.
GeneIDi1443252.
KEGGipho:PH0927.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000001 Genomic DNA. Translation: BAA30023.1.
PIRiA71083.
RefSeqiWP_010885017.1. NC_000961.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2ZU7X-ray2.50A/B1-394[»]
2ZU8X-ray2.40A/B1-394[»]
2ZU9X-ray2.00A/B1-394[»]
ProteinModelPortaliO58689.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi70601.PH0927.

Protein family/group databases

CAZyiGT55. Glycosyltransferase Family 55.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAA30023; BAA30023; BAA30023.
GeneIDi1443252.
KEGGipho:PH0927.

Phylogenomic databases

eggNOGiarCOG04158. Archaea.
ENOG410ZA90. LUCA.
HOGENOMiHOG000246027.
KOiK05947.
OMAiFIDADNY.

Enzyme and pathway databases

UniPathwayiUPA00130; UER00192.
BioCyciMetaCyc:MONOMER-13380.
PHOR70601:GJWR-920-MONOMER.
SABIO-RKO58689.

Miscellaneous databases

EvolutionaryTraceiO58689.

Family and domain databases

InterProiIPR029044. Nucleotide-diphossugar_trans.
IPR012812. Osmo_MPG_synth.
[Graphical view]
PfamiPF09488. Osmo_MPGsynth. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 1 hit.
TIGRFAMsiTIGR02460. osmo_MPGsynth. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3.
  2. "Pathway for the synthesis of mannosylglycerate in the hyperthermophilic archaeon Pyrococcus horikoshii. Biochemical and genetic characterization of key enzymes."
    Empadinhas N., Marugg J.D., Borges N., Santos H., da Costa M.S.
    J. Biol. Chem. 276:43580-43588(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
    Strain: ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3.

Entry informationi

Entry nameiMPGS_PYRHO
AccessioniPrimary (citable) accession number: O58689
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 12, 2003
Last sequence update: August 1, 1998
Last modified: December 9, 2015
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.