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Protein

Mannosyl-3-phosphoglycerate synthase

Gene

mngA

Organism
Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Transfers a mannosyl group from GDP-mannose to phosphoglycerate to form mannosyl-3-phosphoglycerate (MPG). The enzyme is absolutely specific for GDP-mannose and 3-phosphoglycerate, and transfers the mannosyl group with retention of configuration.

Catalytic activityi

GDP-mannose + 3-phospho-D-glycerate = GDP + 2-(alpha-D-mannosyl)-3-phosphoglycerate.

Cofactori

Pathwayi: 2-(alpha-D-mannosyl)-D-glycerate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes 2-(alpha-D-mannosyl)-D-glycerate from GDP-alpha-D-mannose (MPG route).
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Mannosyl-3-phosphoglycerate synthase (mngA)
  2. Mannosyl-3-phosphoglycerate phosphatase (mngB)
This subpathway is part of the pathway 2-(alpha-D-mannosyl)-D-glycerate biosynthesis, which is itself part of Carbohydrate biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 2-(alpha-D-mannosyl)-D-glycerate from GDP-alpha-D-mannose (MPG route), the pathway 2-(alpha-D-mannosyl)-D-glycerate biosynthesis and in Carbohydrate biosynthesis.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Ligandi

Magnesium

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-13380.
SABIO-RKO58689.
UniPathwayiUPA00130; UER00192.

Protein family/group databases

CAZyiGT55. Glycosyltransferase Family 55.

Names & Taxonomyi

Protein namesi
Recommended name:
Mannosyl-3-phosphoglycerate synthase (EC:2.4.1.217)
Short name:
MPG synthase
Short name:
MPGS
Gene namesi
Name:mngA
Ordered Locus Names:PH0927
OrganismiPyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Taxonomic identifieri70601 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
Proteomesi
  • UP000000752 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000592871 – 394Mannosyl-3-phosphoglycerate synthaseAdd BLAST394

Interactioni

Protein-protein interaction databases

STRINGi70601.PH0927.

Structurei

Secondary structure

1394
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi2 – 4Combined sources3
Beta strandi9 – 12Combined sources4
Beta strandi15 – 18Combined sources4
Beta strandi21 – 26Combined sources6
Beta strandi38 – 40Combined sources3
Helixi43 – 50Combined sources8
Beta strandi53 – 61Combined sources9
Helixi64 – 73Combined sources10
Beta strandi80 – 84Combined sources5
Beta strandi89 – 91Combined sources3
Helixi93 – 108Combined sources16
Beta strandi112 – 116Combined sources5
Helixi120 – 128Combined sources9
Beta strandi139 – 141Combined sources3
Helixi145 – 158Combined sources14
Beta strandi162 – 166Combined sources5
Helixi174 – 190Combined sources17
Beta strandi193 – 201Combined sources9
Helixi221 – 237Combined sources17
Beta strandi250 – 255Combined sources6
Helixi256 – 259Combined sources4
Beta strandi264 – 266Combined sources3
Helixi267 – 269Combined sources3
Helixi270 – 282Combined sources13
Beta strandi283 – 285Combined sources3
Helixi289 – 291Combined sources3
Helixi292 – 297Combined sources6
Beta strandi299 – 305Combined sources7
Helixi317 – 332Combined sources16
Helixi339 – 351Combined sources13
Beta strandi370 – 373Combined sources4
Helixi375 – 385Combined sources11
Beta strandi390 – 392Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2ZU7X-ray2.50A/B1-394[»]
2ZU8X-ray2.40A/B1-394[»]
2ZU9X-ray2.00A/B1-394[»]
ProteinModelPortaliO58689.
SMRiO58689.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO58689.

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyltransferase 2 family.Curated

Phylogenomic databases

eggNOGiarCOG04158. Archaea.
ENOG410ZA90. LUCA.
HOGENOMiHOG000246027.
KOiK05947.
OMAiFIDADNY.

Family and domain databases

InterProiIPR029044. Nucleotide-diphossugar_trans.
IPR012812. Osmo_MPG_synth.
[Graphical view]
PfamiPF09488. Osmo_MPGsynth. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 1 hit.
TIGRFAMsiTIGR02460. osmo_MPGsynth. 1 hit.

Sequencei

Sequence statusi: Complete.

O58689-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLLEAPVYKE IFGAVTIHEV QKVIKMDTET EEVPIYTISN IPREKIYDLL
60 70 80 90 100
GKMAVIVPMK NEKLHLVDGV LKAIPHKCPI IIVSNSKREG PNRYKLEVDL
110 120 130 140 150
IRHFYNLTHS KIIMIHQKDP GLAKAFKEVG YTDILDENGM IRSGKGEGML
160 170 180 190 200
VGLLLAKAIG AEYVGFVDAD NYIPGAVNEY VKDYAAGFLM SESEYTMVRL
210 220 230 240 250
HWRHKPKVTK GTLYFKKWGR VSEITNHYLN LLVSEHTAFE TTIMVTGNAG
260 270 280 290 300
EHAMTMKLAE ILPFSTGYSI EPYEIVYILE RFGKWENVEE FKDVFDQGIE
310 320 330 340 350
IFQIETLNPH FHEDKGKEHV KEMLLLSLAT IYHSKLATDN LRKRILKDLR
360 370 380 390
DHGILGENEE PPKPLVMRPI KEIPIKEWMD IVEGNSETLL RFEL
Length:394
Mass (Da):45,210
Last modified:August 1, 1998 - v1
Checksum:i6C2FB4141D2CC807
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000001 Genomic DNA. Translation: BAA30023.1.
PIRiA71083.
RefSeqiWP_010885017.1. NC_000961.1.

Genome annotation databases

EnsemblBacteriaiBAA30023; BAA30023; BAA30023.
GeneIDi1443252.
KEGGipho:PH0927.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000001 Genomic DNA. Translation: BAA30023.1.
PIRiA71083.
RefSeqiWP_010885017.1. NC_000961.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2ZU7X-ray2.50A/B1-394[»]
2ZU8X-ray2.40A/B1-394[»]
2ZU9X-ray2.00A/B1-394[»]
ProteinModelPortaliO58689.
SMRiO58689.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi70601.PH0927.

Protein family/group databases

CAZyiGT55. Glycosyltransferase Family 55.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAA30023; BAA30023; BAA30023.
GeneIDi1443252.
KEGGipho:PH0927.

Phylogenomic databases

eggNOGiarCOG04158. Archaea.
ENOG410ZA90. LUCA.
HOGENOMiHOG000246027.
KOiK05947.
OMAiFIDADNY.

Enzyme and pathway databases

UniPathwayiUPA00130; UER00192.
BioCyciMetaCyc:MONOMER-13380.
SABIO-RKO58689.

Miscellaneous databases

EvolutionaryTraceiO58689.

Family and domain databases

InterProiIPR029044. Nucleotide-diphossugar_trans.
IPR012812. Osmo_MPG_synth.
[Graphical view]
PfamiPF09488. Osmo_MPGsynth. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 1 hit.
TIGRFAMsiTIGR02460. osmo_MPGsynth. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMPGS_PYRHO
AccessioniPrimary (citable) accession number: O58689
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 12, 2003
Last sequence update: August 1, 1998
Last modified: November 2, 2016
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.