O58495 (IORA_PYRHO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Indolepyruvate oxidoreductase subunit iorA
Short name=IOR
EC=1.2.7.8

Alternative name(s):
Indolepyruvate ferredoxin oxidoreductase subunit alpha

Gene names

Name:	iorA
Ordered Locus Names:	PH0765

Organism

Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3) [Complete proteome] [HAMAP]

Taxonomic identifier

70601 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Thermococci › Thermococcales › Thermococcaceae › Pyrococcus

Protein attributes

Sequence length	648 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the ferredoxin-dependent oxidative decarboxylation of arylpyruvates By similarity.
Catalytic activity	(Indol-3-yl)pyruvate + CoA + 2 oxidized ferredoxin = S-2-(indol-3-yl)acetyl-CoA + CO2 + 2 reduced ferredoxin + H+.
Cofactor	Binds 1 4Fe-4S cluster.
Subunit structure	Heterodimer of the iorA and iorB subunits.
Sequence similarities	Contains 2 4Fe-4S ferredoxin-type domains.

Ontologies

Keywords
Biological process	Electron transport Transport
Domain	Repeat
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW transport Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW electron carrier activity Inferred from electronic annotation. Source: InterPro indolepyruvate ferredoxin oxidoreductase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW thiamine pyrophosphate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 648	648	Indolepyruvate oxidoreductase subunit iorA		PRO_0000099929
Regions
	Domain	585 – 614	30	4Fe-4S ferredoxin-type 1
	Domain	616 – 645	30	4Fe-4S ferredoxin-type 2
Sites
	Metal binding	625	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	628	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	631	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	635	1	Iron-sulfur (4Fe-4S) Potential

Sequences

	Sequence		Length	Mass (Da)
	O58495 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: 75A843815FCE08B1	FASTA	648	71,532
	10 20 30 40 50 60 MVKVTDIVLW DKPGEKVLLL GNQAIVRGAL EGNIGVYAAY PGTPSSEITD TMAMVAKKAG 70 80 90 100 110 120 VYMEYSTNEK VAFETALSAS WAGLRAMTAM KHVGLNVAMD SFMTVSYMGI NGGLIVVVAD 130 140 150 160 170 180 DPSMWSSQNE QDTRAIAKFA NIPVLEPSSV QEAKDMVKYA FEISEKYGQM VILRTTTRSS 190 200 210 220 230 240 HMRGDVVLGE LPQEIKEGKR KFGNFKKNPE KYVDIPAFQR PKHPWLLETI EKFREEFNTS 250 260 270 280 290 300 PFNWIEGPED AKVGIIAPGL SYAYVKEALA WLGIDNVKIL KLGTPFPVPY GLLEKFFNGL 310 320 330 340 350 360 ERVLIVEELE PVVEEQVKVW AFDNNINIEI HGKDLVPRVY EMTTRRAVEA VAKFLGVETP 370 380 390 400 410 420 INFQEIDEKY KKVQEMVPPR PPSLCPACPH RNTFFAIRKA ATPRAIFPSD IGCYTLGVLP 430 440 450 460 470 480 PLKTVDTTIA MGGSIGVAHG LSIALNGALG EEQRKTGKEK KIIVATIGDS TFFHTGLPAL 490 500 510 520 530 540 ANAIYNRSNV LIVVMDNMVT AMTGDQPNPG TGETPHGPGK RILIEEVARA MGADFVAVVD 550 560 570 580 590 600 PYDIKETYET IKKALEVEGV SVVVSRRACA LYRIGQLRRE GKQWPIYQVN EEKCTGCKIC 610 620 630 640 INAYGCPAIY WDAEKKKARV DPLMCWGCGG CAQVCPFGAF EKVREGEL

References

[1]

"Complete sequence and gene organization of the genome of a hyper-thermophilic archaebacterium, Pyrococcus horikoshii OT3." Kawarabayasi Y., Sawada M., Horikawa H., Haikawa Y., Hino Y., Yamamoto S., Sekine M., Baba S., Kosugi H., Hosoyama A., Nagai Y., Sakai M., Ogura K., Otsuka R., Nakazawa H., Takamiya M., Ohfuku Y., Funahashi T. , Tanaka T., Kudoh Y., Yamazaki J., Kushida N., Oguchi A., Aoki K., Yoshizawa T., Nakamura Y., Robb F.T., Horikoshi K., Masuchi Y., Shizuya H., Kikuchi H. DNA Res. 5:55-76(1998) [PubMed: 9679194] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000001 Genomic DNA. Translation: BAA29856.1.
PIR	F71124.
RefSeq	NP_142702.1. NC_000961.1.
3D structure databases
ProteinModelPortal	O58495.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBPYRT00000000230; EBPYRP00000000230; EBPYRG00000000230.
GeneID	1443091.
GenomeReviews	Gene locus PH0765 in contig BA000001_GR.
KEGG	pho:PH0765.
NMPDR	fig|70601.1.peg.744.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000022252.
HOGENOM	HBG733885.
OMA	VADDPSM.
ProtClustDB	CLSK785560.
Enzyme and pathway databases
BioCyc	PHOR70601:PH0765-MONOMER.
Family and domain databases
InterPro	IPR001450. 4Fe4S-bd_dom. IPR017896. 4Fe4S_Fe-S-bd. IPR017900. 4Fe4S_Fe_S_CS. IPR017721. Indolepyruvate_Fd_OxRdtase_asu. IPR002880. Pyrv_Fd/Flavodoxin_OxRdtase_N. IPR011766. TPP_enzyme-bd_C. IPR009014. Transketo_C/Pyr-ferredox_oxred. [Graphical view]
KO	K00179.
Pfam	PF12838. Fer4_7. 1 hit. PF01855. POR_N. 1 hit. PF02775. TPP_enzyme_C. 1 hit. [Graphical view]
SUPFAM	SSF52922. Transketo_C_like. 1 hit.
TIGRFAMs	TIGR03336. IOR_alpha. 1 hit.
PROSITE	PS00198. 4FE4S_FER_1. 1 hit. PS51379. 4FE4S_FER_2. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

IORA_PYRHO

Accession

Primary (citable) accession number: O58495

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	August 1, 1998
Last modified:	November 16, 2011
This is version 78 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program