O58489 (AAT_PYRHO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Aspartate aminotransferase
Short name=AspAT
EC=2.6.1.1

Alternative name(s):
Transaminase A

Gene names

Name:	aspC
Ordered Locus Names:	PH0771

Organism

Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3) [Complete proteome] [HAMAP]

Taxonomic identifier

70601 [NCBI]

Taxonomic lineage

cellular organisms › Archaea › Euryarchaeota › Thermococci › Thermococcales › Thermococcaceae › Pyrococcus › Pyrococcus horikoshii

Protein attributes

Sequence length	391 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	L-aspartate + 2-oxoglutarate = oxaloacetate + L-glutamate.
Cofactor	Pyridoxal phosphate By similarity.
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Pyridoxal phosphate
Molecular function	Aminotransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	L-aspartate:2-oxoglutarate aminotransferase activity Inferred from electronic annotation. Source: EC L-phenylalanine:2-oxoglutarate aminotransferase activity Inferred from electronic annotation. Source: EC pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 391	391	Aspartate aminotransferase		PRO_0000123861
Sites
	Binding site	40	1	Aspartate; via amide nitrogen By similarity
	Binding site	176	1	Aspartate By similarity
	Binding site	366	1	Aspartate By similarity
Amino acid modifications
	Modified residue	236	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	O58489 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: C35FD73BD08FE4C1	FASTA	391	44,565
	10 20 30 40 50 60 MREKRKYFIA ERVLLIKRSK IRELFERASK MEDVISLGIG EPDFDTPKNI KEAAKRALDE 70 80 90 100 110 120 GWTHYTPNAG IPELREAVVE YYKKFYGIDI EVENVIITAG AYEGTYLAFE SLLERGDEVI 130 140 150 160 170 180 IPDPAFVSYA EDAKVAEAKP VRIPLREENN FLPDPNELLE KISKNTRMIV INYPNNPTGA 190 200 210 220 230 240 TLDKELAKTI ADIAEDYNIY ILSDEPYEHF IYEDAKHYPM IKFAPENTIL ANSFSKTFAM 250 260 270 280 290 300 TGWRLGFVVA PSQVIKEMTK LHAYVIGNVA SFVQIAGIEA LRSEESWKAV EEMKKEYNER 310 320 330 340 350 360 RKIVVKRLKN MPGIKVKEPK GAFYVFPNIS GTGMSSEKFS EWLLEKARVV VIPGTAFGRM 370 380 390 GEGYVRISYA TSKEKLIEAM NRIEKALEGE K

References

[1]

"Complete sequence and gene organization of the genome of a hyper-thermophilic archaebacterium, Pyrococcus horikoshii OT3." Kawarabayasi Y., Sawada M., Horikawa H., Haikawa Y., Hino Y., Yamamoto S., Sekine M., Baba S., Kosugi H., Hosoyama A., Nagai Y., Sakai M., Ogura K., Otsuka R., Nakazawa H., Takamiya M., Ohfuku Y., Funahashi T. , Tanaka T., Kudoh Y., Yamazaki J., Kushida N., Oguchi A., Aoki K., Yoshizawa T., Nakamura Y., Robb F.T., Horikoshi K., Masuchi Y., Shizuya H., Kikuchi H. DNA Res. 5:55-76(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000001 Genomic DNA. Translation: BAA29863.1.
PIR	E71125.
RefSeq	NP_142708.1. NC_000961.1.
3D structure databases
ProteinModelPortal	O58489.
SMR	O58489. Positions 21-391.
ModBase	Search...
Protein-protein interaction databases
STRING	70601.PH0771.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	BAA29863; BAA29863; BAA29863.
GeneID	1443098.
KEGG	pho:PH0771.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0436.
HOGENOM	HOG000223062.
KO	K00812.
OMA	EGWTHYT.
ProtClustDB	PRK08361.
Enzyme and pathway databases
BioCyc	PHOR70601:GJWR-765-MONOMER.
Family and domain databases
Gene3D	3.40.640.10. 1 hit. 3.90.1150.10. 1 hit.
InterPro	IPR004839. Aminotransferase_I/II. IPR004838. NHTrfase_class1_PyrdxlP-BS. IPR015424. PyrdxlP-dep_Trfase. IPR015421. PyrdxlP-dep_Trfase_major_sub1. IPR015422. PyrdxlP-dep_Trfase_major_sub2. [Graphical view]
Pfam	PF00155. Aminotran_1_2. 1 hit. [Graphical view]
SUPFAM	SSF53383. PyrdxlP-dep_Trfase_major. 1 hit.
PROSITE	PS00105. AA_TRANSFER_CLASS_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AAT_PYRHO

Accession

Primary (citable) accession number: O58489

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 2001
Last sequence update:	August 1, 1998
Last modified:	May 1, 2013
This is version 85 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program