O58466 (RIBL_PYRHO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
FAD synthase
EC=2.7.7.2

Alternative name(s):
FMN adenylyltransferase
Flavin adenine dinucleotide synthase

Gene names

Name:	ribL
Ordered Locus Names:	PH0735

Organism

Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3) [Complete proteome] [HAMAP]

Taxonomic identifier

70601 [NCBI]

Taxonomic lineage

cellular organisms › Archaea › Euryarchaeota › Thermococci › Thermococcales › Thermococcaceae › Pyrococcus › Pyrococcus horikoshii

Protein attributes

Sequence length	148 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the transfer of the AMP portion of ATP to flavin mononucleotide (FMN) to produce flavin adenine dinucleotide (FAD) coenzyme By similarity. HAMAP-Rule MF_02115
Catalytic activity	ATP + FMN = diphosphate + FAD. HAMAP-Rule MF_02115
Cofactor	Divalent metal cations By similarity.
Pathway	Cofactor biosynthesis; FAD biosynthesis; FAD from FMN: step 1/1. HAMAP-Rule MF_02115
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the archaeal FAD synthase family.

Ontologies

Keywords
Ligand	ATP-binding FAD FMN Nucleotide-binding
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	FAD biosynthetic process Inferred from electronic annotation. Source: HAMAP FMN metabolic process Inferred from electronic annotation. Source: HAMAP
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP FMN adenylyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 148	148	FAD synthase HAMAP-Rule MF_02115		PRO_0000406281
Regions
	Nucleotide binding	14 – 15	2	ATP By similarity
	Nucleotide binding	19 – 22	4	ATP By similarity
	Nucleotide binding	98 – 101	4	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	O58466 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: 07F566C19188FE38	FASTA	148	16,737
	10 20 30 40 50 60 MGSDRKIRVV VGGVFDIIHA GHVHFLKMAK ELGDELIVIV AHDETVKKRK GRPPINPAED 70 80 90 100 110 120 RAEVLKAIRY VDDVVIGKPG EISLDLIKRL KPDVIALGPD QDFDCEDLKR KLKSIGLNVE 130 140 VIRLPYLYKK DRAKTSKIIE RITEIFCD

References

[1]

"Complete sequence and gene organization of the genome of a hyper-thermophilic archaebacterium, Pyrococcus horikoshii OT3." Kawarabayasi Y., Sawada M., Horikawa H., Haikawa Y., Hino Y., Yamamoto S., Sekine M., Baba S., Kosugi H., Hosoyama A., Nagai Y., Sakai M., Ogura K., Otsuka R., Nakazawa H., Takamiya M., Ohfuku Y., Funahashi T. , Tanaka T., Kudoh Y., Yamazaki J., Kushida N., Oguchi A., Aoki K., Yoshizawa T., Nakamura Y., Robb F.T., Horikoshi K., Masuchi Y., Shizuya H., Kikuchi H. DNA Res. 5:55-76(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000001 Genomic DNA. Translation: BAA29826.1.
PIR	H71120.
RefSeq	NP_142678.1. NC_000961.1.
3D structure databases
HSSP	HSSP built from PDB template 1COZ based on UniProtKB P27623.
ProteinModelPortal	O58466.
ModBase	Search...
Protein-protein interaction databases
STRING	70601.PH0735.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	BAA29826; BAA29826; BAA29826.
GeneID	1443066.
KEGG	pho:PH0735.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0615.
HOGENOM	HOG000284153.
KO	K14656.
OMA	VAHDETV.
ProtClustDB	CLSK253517.
Enzyme and pathway databases
BioCyc	PHOR70601:GJWR-732-MONOMER.
UniPathway	UPA00277; UER00407.
Family and domain databases
Gene3D	3.40.50.620. 1 hit.
HAMAP	MF_02115. FAD_synth_arch.
InterPro	IPR004821. Cyt_trans-like. IPR024902. FAD_synth_RibL. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Pfam	PF01467. CTP_transf_2. 1 hit. [Graphical view]
TIGRFAMs	TIGR00125. cyt_tran_rel. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RIBL_PYRHO

Accession

Primary (citable) accession number: O58466

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 5, 2011
Last sequence update:	August 1, 1998
Last modified:	May 1, 2013
This is version 73 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program