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Protein

Ornithine carbamoyltransferase

Gene

argF

Organism
Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Reversibly catalyzes the transfer of the carbamoyl group from carbamoyl phosphate (CP) to the N(epsilon) atom of ornithine (ORN) to produce L-citrulline.By similarity

Catalytic activityi

Carbamoyl phosphate + L-ornithine = phosphate + L-citrulline.UniRule annotation

Pathwayi: L-arginine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes L-arginine from L-ornithine and carbamoyl phosphate.UniRule annotation
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Ornithine carbamoyltransferase (argF)
  2. no protein annotated in this organism
  3. no protein annotated in this organism
This subpathway is part of the pathway L-arginine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-arginine from L-ornithine and carbamoyl phosphate, the pathway L-arginine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei11Carbamoyl phosphateUniRule annotation1
Binding sitei84Carbamoyl phosphateUniRule annotation1
Binding sitei108Carbamoyl phosphateUniRule annotation1
Binding sitei166OrnithineBy similarity1
Binding sitei230OrnithineBy similarity1
Binding sitei280Carbamoyl phosphateUniRule annotation1
Binding sitei298Carbamoyl phosphateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Amino-acid biosynthesis, Arginine biosynthesis

Enzyme and pathway databases

UniPathwayiUPA00068; UER00112.

Names & Taxonomyi

Protein namesi
Recommended name:
Ornithine carbamoyltransferaseUniRule annotation (EC:2.1.3.3UniRule annotation)
Short name:
OTCaseUniRule annotation
Gene namesi
Name:argFUniRule annotation
Ordered Locus Names:PH0726
OrganismiPyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Taxonomic identifieri70601 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
Proteomesi
  • UP000000752 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001130742 – 317Ornithine carbamoyltransferaseAdd BLAST316

Interactioni

Subunit structurei

Homododecamer.By similarity

Protein-protein interaction databases

STRINGi70601.PH0726.

Structurei

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei32Important for structural integrityUniRule annotation1
Sitei148Important for structural integrityUniRule annotation1

3D structure databases

ProteinModelPortaliO58457.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni57 – 61Carbamoyl phosphate bindingUniRule annotation5
Regioni135 – 138Carbamoyl phosphate bindingUniRule annotation4
Regioni234 – 235Ornithine bindingUniRule annotation2
Regioni269 – 272Carbamoyl phosphate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the ATCase/OTCase family.UniRule annotation

Phylogenomic databases

eggNOGiarCOG00912. Archaea.
COG0078. LUCA.
HOGENOMiHOG000022686.
KOiK00611.
OMAiEHPCQIL.

Family and domain databases

Gene3Di3.40.50.1370. 2 hits.
HAMAPiMF_01109. OTCase. 1 hit.
InterProiIPR006132. Asp/Orn_carbamoyltranf_P-bd.
IPR006130. Asp/Orn_carbamoylTrfase.
IPR006131. Asp_carbamoyltransf_Asp/Orn-bd.
IPR002292. Orn/put_carbamltrans.
IPR024904. OTCase_ArgI.
[Graphical view]
PfamiPF00185. OTCace. 1 hit.
PF02729. OTCace_N. 1 hit.
[Graphical view]
PRINTSiPR00100. AOTCASE.
PR00102. OTCASE.
SUPFAMiSSF53671. SSF53671. 1 hit.
TIGRFAMsiTIGR00658. orni_carb_tr. 1 hit.
PROSITEiPS00097. CARBAMOYLTRANSFERASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O58457-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVVSLKGRDL LCLQDYTPEE IWTILETAKM FKIWQKIGKP HRLLEGKTLA
60 70 80 90 100
MIFQKPSTRT RVSFEVAMAH LGGHALYLNA QDLQLRRGET IADTARVLSR
110 120 130 140 150
YVDAIMARVY AHKDVEDLAK YASVPVINGL SDFSHPCQAL ADYMTIWEKK
160 170 180 190 200
GTIKGVKVVY VGDGNNVCHS LMIAGTKLGA DVVVATPEGY EPDKKVIKWA
210 220 230 240 250
EQNAAESGGS FELLHDPVKA VKDADVIYTD VWASMGQEAE AEERRKIFRP
260 270 280 290 300
FQVNKDLVKH AKSDYMFMHC LPAHRGEEVT DDVIDSPNSV VWDEAENRLH
310
AQKAVLALLL GGVKTGF
Length:317
Mass (Da):35,369
Last modified:January 23, 2007 - v3
Checksum:iD949989E0E8F6B1E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000001 Genomic DNA. Translation: BAA29817.1.
PIRiG71119.
RefSeqiWP_010884824.1. NC_000961.1.

Genome annotation databases

EnsemblBacteriaiBAA29817; BAA29817; BAA29817.
GeneIDi1443059.
KEGGipho:PH0726.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000001 Genomic DNA. Translation: BAA29817.1.
PIRiG71119.
RefSeqiWP_010884824.1. NC_000961.1.

3D structure databases

ProteinModelPortaliO58457.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi70601.PH0726.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAA29817; BAA29817; BAA29817.
GeneIDi1443059.
KEGGipho:PH0726.

Phylogenomic databases

eggNOGiarCOG00912. Archaea.
COG0078. LUCA.
HOGENOMiHOG000022686.
KOiK00611.
OMAiEHPCQIL.

Enzyme and pathway databases

UniPathwayiUPA00068; UER00112.

Family and domain databases

Gene3Di3.40.50.1370. 2 hits.
HAMAPiMF_01109. OTCase. 1 hit.
InterProiIPR006132. Asp/Orn_carbamoyltranf_P-bd.
IPR006130. Asp/Orn_carbamoylTrfase.
IPR006131. Asp_carbamoyltransf_Asp/Orn-bd.
IPR002292. Orn/put_carbamltrans.
IPR024904. OTCase_ArgI.
[Graphical view]
PfamiPF00185. OTCace. 1 hit.
PF02729. OTCace_N. 1 hit.
[Graphical view]
PRINTSiPR00100. AOTCASE.
PR00102. OTCASE.
SUPFAMiSSF53671. SSF53671. 1 hit.
TIGRFAMsiTIGR00658. orni_carb_tr. 1 hit.
PROSITEiPS00097. CARBAMOYLTRANSFERASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiOTC_PYRHO
AccessioniPrimary (citable) accession number: O58457
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: January 23, 2007
Last modified: November 2, 2016
This is version 114 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.