O58451 (PYRB_PYRHO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Aspartate carbamoyltransferase
EC=2.1.3.2

Alternative name(s):
Aspartate transcarbamylase
Short name=ATCase

Gene names

Name:	pyrB
Ordered Locus Names:	PH0720

Organism

Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3) [Complete proteome] [HAMAP]

Taxonomic identifier

70601 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Thermococci › Thermococcales › Thermococcaceae › Pyrococcus

Protein attributes

Sequence length	308 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Carbamoyl phosphate + L-aspartate = phosphate + N-carbamoyl-L-aspartate. HAMAP MF_00001
Pathway	Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 2/3. HAMAP MF_00001
Subunit structure	Heterooligomer of catalytic and regulatory chains.
Sequence similarities	Belongs to the ATCase/OTCase family.

Ontologies

Keywords
Biological process	Pyrimidine biosynthesis
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	'de novo' pyrimidine base biosynthetic process Inferred from electronic annotation. Source: InterPro cellular amino acid metabolic process Inferred from electronic annotation. Source: InterPro pyrimidine nucleotide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	amino acid binding Inferred from electronic annotation. Source: InterPro aspartate carbamoyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 308	308	Aspartate carbamoyltransferase HAMAP MF_00001		PRO_0000113256

Sequences

	Sequence		Length	Mass (Da)
	O58451 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: E20A6A29F5B25B27	FASTA	308	34,862
	10 20 30 40 50 60 MEWKGRDVIS IRDFSKEDIE VVLSTAERLE KEMKEKGQLE YAKGKILATL FFEPSTRTRL 70 80 90 100 110 120 SFESAMHRLG GSVIGFAEAS TSSVKKGESL RDTIKTVEQY SDVIVIRHPK EGAARLAAEV 130 140 150 160 170 180 ADIPVINAGD GSNQHPTQTL LDLYTIKKEF GTIDGLKIGL LGDLKYGRTV HSLAEALAFY 190 200 210 220 230 240 DVELYLISPE LLRMPKHIVE ELRERGMKIV ETTKLEEVIG ELDVLYVTRI QKERFPDEQE 250 260 270 280 290 300 YLKVKGSYQV NLKILENVKD SLRIMHPLPR VDEIHPEVDK TKHAIYFKQV FNGVPVRMAL LALVLGVI

References

[1]

"Complete sequence and gene organization of the genome of a hyper-thermophilic archaebacterium, Pyrococcus horikoshii OT3." Kawarabayasi Y., Sawada M., Horikawa H., Haikawa Y., Hino Y., Yamamoto S., Sekine M., Baba S., Kosugi H., Hosoyama A., Nagai Y., Sakai M., Ogura K., Otsuka R., Nakazawa H., Takamiya M., Ohfuku Y., Funahashi T. , Tanaka T., Kudoh Y., Yamazaki J., Kushida N., Oguchi A., Aoki K., Yoshizawa T., Nakamura Y., Robb F.T., Horikoshi K., Masuchi Y., Shizuya H., Kikuchi H. DNA Res. 5:55-76(1998) [PubMed: 9679194] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000001 Genomic DNA. Translation: BAA29811.1.
PIR	A71119.
RefSeq	NP_142665.1. NC_000961.1.
3D structure databases
ProteinModelPortal	O58451.
SMR	O58451. Positions 2-308.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBPYRT00000000338; EBPYRP00000000338; EBPYRG00000000338.
GeneID	1443053.
GenomeReviews	Gene locus PH0720 in contig BA000001_GR.
KEGG	pho:PH0720.
NMPDR	fig|70601.1.peg.706.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000022978.
HOGENOM	HBG579429.
OMA	FKQSFYG.
PhylomeDB	O58451.
ProtClustDB	PRK00856.
Enzyme and pathway databases
BioCyc	PHOR70601:PH0720-MONOMER.
Family and domain databases
HAMAP	MF_00001. Asp_carb_tr. [Tree]
InterPro	IPR006132. Asp/Orn_carbamoyltranf_P-bd. IPR006130. Asp/Orn_carbamoylTrfase. IPR006131. Asp_carbamoyltransf_Asp/Orn-bd. IPR002082. Asp_carbamoyltransf_euk. [Graphical view]
KO	K00609.
Pfam	PF00185. OTCace. 1 hit. PF02729. OTCace_N. 1 hit. [Graphical view]
PRINTS	PR00100. AOTCASE. PR00101. ATCASE.
SUPFAM	SSF53671. Asp/Orn_carbamoyltranf. 1 hit.
TIGRFAMs	TIGR00670. Asp_carb_tr. 1 hit.
PROSITE	PS00097. CARBAMOYLTRANSFERASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PYRB_PYRHO

Accession

Primary (citable) accession number: O58451

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	August 1, 1998
Last modified:	December 14, 2011
This is version 81 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program