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Protein

Threonine--tRNA ligase

Gene

thrS

Organism
Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the attachment of threonine to tRNA(Thr) in a two-step reaction: L-threonine is first activated by ATP to form Thr-AMP and then transferred to the acceptor end of tRNA(Thr). Also edits incorrectly charged L-seryl-tRNA(Thr).

Catalytic activityi

ATP + L-threonine + tRNA(Thr) = AMP + diphosphate + L-threonyl-tRNA(Thr).

Cofactori

Zn2+Note: Binds 1 zinc ion per subunit.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi298Zinc1
Metal bindingi350Zinc; via tele nitrogen1
Metal bindingi474Zinc; via pros nitrogen1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase, RNA-binding, tRNA-binding
Biological processProtein biosynthesis
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Threonine--tRNA ligase (EC:6.1.1.3)
Alternative name(s):
Threonyl-tRNA synthetase
Short name:
ThrRS
Gene namesi
Name:thrS
Ordered Locus Names:PH0699
OrganismiPyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Taxonomic identifieri70601 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
Proteomesi
  • UP000000752 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001011101 – 625Threonine--tRNA ligaseAdd BLAST625

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

STRINGi70601.PH0699.

Structurei

3D structure databases

ProteinModelPortaliO58430.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 143Editing domainAdd BLAST143
Regioni206 – 505CatalyticAdd BLAST300

Domaini

The N-terminal domain is an archaea-specific tRNA-editing domain that hydrolyzes incorrectly charged L-seryl-tRNA(Thr). Catalysis of tRNA editing is performed by the charged tRNA itself.

Sequence similaritiesi

Belongs to the class-II aminoacyl-tRNA synthetase family.

Phylogenomic databases

eggNOGiarCOG00401. Archaea.
COG0441. LUCA.
HOGENOMiHOG000073571.
KOiK01868.
OMAiDAHIFML.
OrthoDBiPOG093Z01GO.

Family and domain databases

Gene3Di3.40.50.800. 1 hit.
HAMAPiMF_00184. Thr_tRNA_synth. 1 hit.
InterProiView protein in InterPro
IPR002314. aa-tRNA-synt_IIb.
IPR006195. aa-tRNA-synth_II.
IPR004154. Anticodon-bd.
IPR036621. Anticodon-bd_dom_sf.
IPR002320. Thr-tRNA-ligase_IIa.
IPR015011. Threonyl-tRNA_syn_edit_dom_arc.
PANTHERiPTHR11451. PTHR11451. 1 hit.
PfamiView protein in Pfam
PF03129. HGTP_anticodon. 1 hit.
PF00587. tRNA-synt_2b. 1 hit.
PF08915. tRNA-Thr_ED. 1 hit.
PRINTSiPR01047. TRNASYNTHTHR.
ProDomiView protein in ProDom or Entries sharing at least one domain
PD016189. tRNA-Thr_ED_arc. 1 hit.
SUPFAMiSSF52954. SSF52954. 1 hit.
TIGRFAMsiTIGR00418. thrS. 1 hit.
PROSITEiView protein in PROSITE
PS50862. AA_TRNA_LIGASE_II. 1 hit.

Sequencei

Sequence statusi: Complete.

O58430-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRILLIHSDY IEYEVKDKAI KNPEPISEEE KKGRMDEVLV AFISVEKVDE
60 70 80 90 100
TNPEEVVSKA VEEIINVASQ VKAENVFVYP FAHLSSELAK PSVAQEILRK
110 120 130 140 150
VYEGLKEKGY NVGKAPFGYY KAFKISCKGH PLAELSRTII PEGAKEEEVP
160 170 180 190 200
EALKKEETEL VSYWYILTPE GELIEVDKFD FTGYENLRKF ANYEISKSRI
210 220 230 240 250
AEKEPPHVKL MLEHELVDYE PGSDPGNLRY YPKGRLIKSL LEQYVTEKVI
260 270 280 290 300
EYGAMEVETP VMYDFEHPAL EKYLNRFPAR QYIVLSGDKK YFLRFAACFG
310 320 330 340 350
QFLISKDAVI SYRHLPLRMY ELTRYSFRRE KRGELSGLRR LRAFTMPDMH
360 370 380 390 400
TLAKDLEQAK DEFKKQFKLS MEVLKGVGLT PEDYEVAIRF TEDFWKENRD
410 420 430 440 450
FIVELVKIIG KPVLIEMWKQ RFFYFILKFE FNFVDNLDKA AALSTVQIDV
460 470 480 490 500
ENAERFGIKY YDENGEEKYP LILHCSPSGA IERVMYAILE KQAKLMQEGK
510 520 530 540 550
KPMLPLWLSP IQVRVIPVSE EYLDYALYIA GKLEGARIRV DVDDEDERLN
560 570 580 590 600
KKIRRAEKEW IPYIVVVGAK EKESGTITVR RREDGKQYET RLEELIKEIK
610 620
EKVEGFPYKP RPLPLLLSKR PKFRG
Length:625
Mass (Da):73,023
Last modified:August 1, 1998 - v1
Checksum:i59A75F409230E2CD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000001 Genomic DNA. Translation: BAA29790.1.
PIRiD71116.
RefSeqiWP_010884794.1. NC_000961.1.

Genome annotation databases

EnsemblBacteriaiBAA29790; BAA29790; BAA29790.
GeneIDi1443029.
KEGGipho:PH0699.

Similar proteinsi

Entry informationi

Entry nameiSYT_PYRHO
AccessioniPrimary (citable) accession number: O58430
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: August 1, 1998
Last modified: October 25, 2017
This is version 120 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families