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Protein

Threonine--tRNA ligase

Gene

thrS

Organism
Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-threonine + tRNA(Thr) = AMP + diphosphate + L-threonyl-tRNA(Thr).

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi298Zinc; catalyticBy similarity1
Metal bindingi350Zinc; catalyticBy similarity1
Metal bindingi474Zinc; catalyticBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Threonine--tRNA ligase (EC:6.1.1.3)
Alternative name(s):
Threonyl-tRNA synthetase
Short name:
ThrRS
Gene namesi
Name:thrS
Ordered Locus Names:PH0699
OrganismiPyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Taxonomic identifieri70601 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
Proteomesi
  • UP000000752 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001011101 – 625Threonine--tRNA ligaseAdd BLAST625

Interactioni

Protein-protein interaction databases

STRINGi70601.PH0699.

Structurei

3D structure databases

ProteinModelPortaliO58430.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni206 – 505CatalyticAdd BLAST300

Sequence similaritiesi

Phylogenomic databases

eggNOGiarCOG00401. Archaea.
COG0441. LUCA.
HOGENOMiHOG000073571.
KOiK01868.
OMAiMYELTRY.

Family and domain databases

Gene3Di3.40.50.800. 1 hit.
HAMAPiMF_00184. Thr_tRNA_synth. 1 hit.
InterProiIPR002314. aa-tRNA-synt_IIb.
IPR006195. aa-tRNA-synth_II.
IPR004154. Anticodon-bd.
IPR002320. Thr-tRNA-ligase_IIa.
IPR015011. Threonyl-tRNA_syn_edit_dom_arc.
[Graphical view]
PfamiPF03129. HGTP_anticodon. 1 hit.
PF00587. tRNA-synt_2b. 1 hit.
PF08915. tRNA-Thr_ED. 1 hit.
[Graphical view]
PRINTSiPR01047. TRNASYNTHTHR.
ProDomiPD016189. tRNA-Thr_ED_arc. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF52954. SSF52954. 1 hit.
TIGRFAMsiTIGR00418. thrS. 1 hit.
PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O58430-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRILLIHSDY IEYEVKDKAI KNPEPISEEE KKGRMDEVLV AFISVEKVDE
60 70 80 90 100
TNPEEVVSKA VEEIINVASQ VKAENVFVYP FAHLSSELAK PSVAQEILRK
110 120 130 140 150
VYEGLKEKGY NVGKAPFGYY KAFKISCKGH PLAELSRTII PEGAKEEEVP
160 170 180 190 200
EALKKEETEL VSYWYILTPE GELIEVDKFD FTGYENLRKF ANYEISKSRI
210 220 230 240 250
AEKEPPHVKL MLEHELVDYE PGSDPGNLRY YPKGRLIKSL LEQYVTEKVI
260 270 280 290 300
EYGAMEVETP VMYDFEHPAL EKYLNRFPAR QYIVLSGDKK YFLRFAACFG
310 320 330 340 350
QFLISKDAVI SYRHLPLRMY ELTRYSFRRE KRGELSGLRR LRAFTMPDMH
360 370 380 390 400
TLAKDLEQAK DEFKKQFKLS MEVLKGVGLT PEDYEVAIRF TEDFWKENRD
410 420 430 440 450
FIVELVKIIG KPVLIEMWKQ RFFYFILKFE FNFVDNLDKA AALSTVQIDV
460 470 480 490 500
ENAERFGIKY YDENGEEKYP LILHCSPSGA IERVMYAILE KQAKLMQEGK
510 520 530 540 550
KPMLPLWLSP IQVRVIPVSE EYLDYALYIA GKLEGARIRV DVDDEDERLN
560 570 580 590 600
KKIRRAEKEW IPYIVVVGAK EKESGTITVR RREDGKQYET RLEELIKEIK
610 620
EKVEGFPYKP RPLPLLLSKR PKFRG
Length:625
Mass (Da):73,023
Last modified:August 1, 1998 - v1
Checksum:i59A75F409230E2CD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000001 Genomic DNA. Translation: BAA29790.1.
PIRiD71116.
RefSeqiWP_010884794.1. NC_000961.1.

Genome annotation databases

EnsemblBacteriaiBAA29790; BAA29790; BAA29790.
GeneIDi1443029.
KEGGipho:PH0699.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000001 Genomic DNA. Translation: BAA29790.1.
PIRiD71116.
RefSeqiWP_010884794.1. NC_000961.1.

3D structure databases

ProteinModelPortaliO58430.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi70601.PH0699.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAA29790; BAA29790; BAA29790.
GeneIDi1443029.
KEGGipho:PH0699.

Phylogenomic databases

eggNOGiarCOG00401. Archaea.
COG0441. LUCA.
HOGENOMiHOG000073571.
KOiK01868.
OMAiMYELTRY.

Family and domain databases

Gene3Di3.40.50.800. 1 hit.
HAMAPiMF_00184. Thr_tRNA_synth. 1 hit.
InterProiIPR002314. aa-tRNA-synt_IIb.
IPR006195. aa-tRNA-synth_II.
IPR004154. Anticodon-bd.
IPR002320. Thr-tRNA-ligase_IIa.
IPR015011. Threonyl-tRNA_syn_edit_dom_arc.
[Graphical view]
PfamiPF03129. HGTP_anticodon. 1 hit.
PF00587. tRNA-synt_2b. 1 hit.
PF08915. tRNA-Thr_ED. 1 hit.
[Graphical view]
PRINTSiPR01047. TRNASYNTHTHR.
ProDomiPD016189. tRNA-Thr_ED_arc. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF52954. SSF52954. 1 hit.
TIGRFAMsiTIGR00418. thrS. 1 hit.
PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSYT_PYRHO
AccessioniPrimary (citable) accession number: O58430
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: August 1, 1998
Last modified: November 2, 2016
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.