ID   VORB_PYRHO              Reviewed;         311 AA.
AC   O58414;
DT   30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT   30-MAY-2000, sequence version 2.
DT   05-MAY-2009, entry version 45.
DE   RecName: Full=Ketoisovalerate oxidoreductase subunit vorB;
DE            Short=VOR;
DE            EC=1.2.7.7;
DE   AltName: Full=2-oxoisovalerate oxidoreductase beta chain;
DE   AltName: Full=2-oxoisovalerate ferredoxin reductase subunit beta;
GN   Name=vorB; OrderedLocusNames=PH0681;
OS   Pyrococcus horikoshii.
OC   Archaea; Euryarchaeota; Thermococci; Thermococcales; Thermococcaceae;
OC   Pyrococcus.
OX   NCBI_TaxID=53953;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=OT3;
RX   MEDLINE=98344137; PubMed=9679194; DOI=10.1093/dnares/5.2.55;
RA   Kawarabayasi Y., Sawada M., Horikawa H., Haikawa Y., Hino Y.,
RA   Yamamoto S., Sekine M., Baba S., Kosugi H., Hosoyama A., Nagai Y.,
RA   Sakai M., Ogura K., Otsuka R., Nakazawa H., Takamiya M., Ohfuku Y.,
RA   Funahashi T., Tanaka T., Kudoh Y., Yamazaki J., Kushida N., Oguchi A.,
RA   Aoki K., Yoshizawa T., Nakamura Y., Robb F.T., Horikoshi K.,
RA   Masuchi Y., Shizuya H., Kikuchi H.;
RT   "Complete sequence and gene organization of the genome of a hyper-
RT   thermophilic archaebacterium, Pyrococcus horikoshii OT3.";
RL   DNA Res. 5:55-76(1998).
CC   -!- CATALYTIC ACTIVITY: 3-methyl-2-oxobutanoate + CoA + 2 oxidized
CC       ferredoxin = S-(2-methylpropanoyl)-CoA + CO(2) + 2 reduced
CC       ferredoxin + H(+).
CC   -!- SUBUNIT: Heterotetramer of one alpha, one beta, one delta and one
CC       gamma chain (By similarity).
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DR   EMBL; BA000001; BAA29772.1; ALT_INIT; Genomic_DNA.
DR   PIR; B71114; B71114.
DR   RefSeq; NP_142631.1; -.
DR   HSSP; P94692; 1KEK.
DR   GeneID; 1443009; -.
DR   GenomeReviews; BA000001_GR; PH0681.
DR   KEGG; pho:PH0681; -.
DR   NMPDR; fig|70601.1.peg.662; -.
DR   HOGENOM; O58414; -.
DR   OMA; O58414; PACCSTI.
DR   BRENDA; 1.2.7.7; 74679.
DR   GO; GO:0043807; F:3-methyl-2-oxobutanoate dehydrogenase (ferr...; IEA:EC.
DR   GO; GO:0030976; F:thiamin pyrophosphate binding; IEA:InterPro.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   InterPro; IPR011766; TPP_enzyme_bd_C.
DR   Pfam; PF02775; TPP_enzyme_C; 1.
PE   3: Inferred from homology;
KW   Complete proteome; Oxidoreductase.
FT   CHAIN         1    311       Ketoisovalerate oxidoreductase subunit
FT                                vorB.
FT                                /FTId=PRO_0000099958.
SQ   SEQUENCE   311 AA;  34569 MW;  E27B65877EF65813 CRC64;
     MEIPEEVKKR LTIPFEENFF AGHTACQGCG ASLGLRYVLK AYGRKTIVVI PACCSTIIAG
     PWPYSALNAN LFHTAFATTG AVISGIEAGL KALGYKVKGE DGIMVVGWAG DGGTADIGLQ
     ALSGFIERGH DAVYIMYDNE AYMNTGIQRS SSTPYGAWTT NTPGGKRHLL EKRHKKKVID
     IVIAHRIPYA ATASVAYPED FIRKLKKAQK IPGPSFIQLF APCPTGWRAP TDKSIEIARL
     AVQTAYFPLF EYENGKYKIN MPNPKKEPKP IEEFLKLQGR FKYMTKEDVE ALQKWVLEEW
     ERLKKLAEVF G
//