O58414 (VORB_PYRHO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ketoisovalerate oxidoreductase subunit vorB
Short name=VOR
EC=1.2.7.7

Alternative name(s):
2-oxoisovalerate ferredoxin reductase subunit beta
2-oxoisovalerate oxidoreductase beta chain

Gene names

Name:	vorB
Ordered Locus Names:	PH0681

Organism

Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3) [Complete proteome] [HAMAP]

Taxonomic identifier

70601 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Thermococci › Thermococcales › Thermococcaceae › Pyrococcus

Protein attributes

Sequence length	311 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	3-methyl-2-oxobutanoate + CoA + 2 oxidized ferredoxin = S-(2-methylpropanoyl)-CoA + CO2 + 2 reduced ferredoxin + H+.
Subunit structure	Heterotetramer of one alpha, one beta, one delta and one gamma chain By similarity.
Sequence caution	The sequence BAA29772.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Molecular function	3-methyl-2-oxobutanoate dehydrogenase (ferredoxin) activity Inferred from electronic annotation. Source: EC thiamine pyrophosphate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 311	311	Ketoisovalerate oxidoreductase subunit vorB		PRO_0000099958

Sequences

	Sequence		Length	Mass (Da)
	O58414 [UniParc]. Last modified May 30, 2000. Version 2. Checksum: E27B65877EF65813	FASTA	311	34,569
	10 20 30 40 50 60 MEIPEEVKKR LTIPFEENFF AGHTACQGCG ASLGLRYVLK AYGRKTIVVI PACCSTIIAG 70 80 90 100 110 120 PWPYSALNAN LFHTAFATTG AVISGIEAGL KALGYKVKGE DGIMVVGWAG DGGTADIGLQ 130 140 150 160 170 180 ALSGFIERGH DAVYIMYDNE AYMNTGIQRS SSTPYGAWTT NTPGGKRHLL EKRHKKKVID 190 200 210 220 230 240 IVIAHRIPYA ATASVAYPED FIRKLKKAQK IPGPSFIQLF APCPTGWRAP TDKSIEIARL 250 260 270 280 290 300 AVQTAYFPLF EYENGKYKIN MPNPKKEPKP IEEFLKLQGR FKYMTKEDVE ALQKWVLEEW 310 ERLKKLAEVF G

References

[1]

"Complete sequence and gene organization of the genome of a hyper-thermophilic archaebacterium, Pyrococcus horikoshii OT3." Kawarabayasi Y., Sawada M., Horikawa H., Haikawa Y., Hino Y., Yamamoto S., Sekine M., Baba S., Kosugi H., Hosoyama A., Nagai Y., Sakai M., Ogura K., Otsuka R., Nakazawa H., Takamiya M., Ohfuku Y., Funahashi T. , Tanaka T., Kudoh Y., Yamazaki J., Kushida N., Oguchi A., Aoki K., Yoshizawa T., Nakamura Y., Robb F.T., Horikoshi K., Masuchi Y., Shizuya H., Kikuchi H. DNA Res. 5:55-76(1998) [PubMed: 9679194] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000001 Genomic DNA. Translation: BAA29772.1. Different initiation.
PIR	B71114.
RefSeq	NP_142631.1. NC_000961.1.
3D structure databases
ProteinModelPortal	O58414.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBPYRT00000000354; EBPYRP00000000354; EBPYRG00000000354.
GeneID	1443009.
GenomeReviews	Gene locus PH0681 in contig BA000001_GR.
KEGG	pho:PH0681.
NMPDR	fig|70601.1.peg.662.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000022253.
HOGENOM	HBG313203.
OMA	HKKKVID.
ProtClustDB	PRK11864.
Enzyme and pathway databases
BioCyc	PHOR70601:PH0681-MONOMER.
Family and domain databases
InterPro	IPR011766. TPP_enzyme-bd_C. [Graphical view]
KO	K00187.
Pfam	PF02775. TPP_enzyme_C. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

VORB_PYRHO

Accession

Primary (citable) accession number: O58414

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	May 30, 2000
Last modified:	November 16, 2011
This is version 64 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program