Reviewed, UniProtKB/Swiss-Prot O58412 (VORD_PYRHO)

Names and origin

Protein names

Recommended name:
    Ketoisovalerate oxidoreductase subunit vorD
      Short name=VOR
    EC=1.2.7.7
Alternative name(s):
    2-oxoisovalerate oxidoreductase delta chain
    2-oxoisovalerate ferredoxin reductase subunit delta

Gene names

Name:	vorD
Ordered Locus Names:	PH0679

Organism

Pyrococcus horikoshii [Complete proteome] [HAMAP]

Taxonomic identifier

53953 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Thermococci › Thermococcales › Thermococcaceae › Pyrococcus

Protein attributes

Sequence length	105 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	3-methyl-2-oxobutanoate + CoA + 2 oxidized ferredoxin = S-(2-methylpropanoyl)-CoA + CO(2) + 2 reduced ferredoxin + H(+).
Cofactor	Binds 2 4Fe-4S clusters By similarity.
Subunit structure	Heterotetramer of one alpha, one beta, one delta and one gamma chain By similarity.
Sequence similarities	Contains 2 4Fe-4S ferredoxin-type domains.

Ontologies

Keywords
Biological process	Electron transport Transport
Domain	Repeat
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW transport Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	3-methyl-2-oxobutanoate dehydrogenase (ferredoxin) activity Inferred from electronic annotation. Source: EC 4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: InterPro electron carrier activity Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 105	105	Ketoisovalerate oxidoreductase subunit vorD		PRO_0000099963
Regions
	Domain	44 – 73	30	4Fe-4S ferredoxin-type 1
	Domain	74 – 103	30	4Fe-4S ferredoxin-type 2
Sites
	Metal binding	53	1	Iron-sulfur 1 (4Fe-4S) Potential
	Metal binding	56	1	Iron-sulfur 1 (4Fe-4S) Potential
	Metal binding	59	1	Iron-sulfur 1 (4Fe-4S) Potential
	Metal binding	63	1	Iron-sulfur 2 (4Fe-4S) Potential
	Metal binding	83	1	Iron-sulfur 2 (4Fe-4S) Potential
	Metal binding	86	1	Iron-sulfur 2 (4Fe-4S) Potential
	Metal binding	89	1	Iron-sulfur 2 (4Fe-4S) Potential
	Metal binding	93	1	Iron-sulfur 1 (4Fe-4S) Potential

Sequences

	Sequence		Length	Mass (Da)
	O58412-1 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: 954AA31F09653FA1	FASTA	105	11,861
	10 20 30 40 50 60 MNTLFGKAKE EAKPITPKSV DEYPEAPVSL GITLVNFTGD WRTFMPVIDE SKCVKCYICW 70 80 90 100 KYCPEPAIYI KEDGFVAIDY DYCKGCGICA NECPTKAITM VREEK

References

[1]

"Complete sequence and gene organization of the genome of a hyper-thermophilic archaebacterium, Pyrococcus horikoshii OT3." Kawarabayasi Y., Sawada M., Horikawa H., Haikawa Y., Hino Y., Yamamoto S., Sekine M., Baba S., Kosugi H., Hosoyama A., Nagai Y., Sakai M., Ogura K., Otsuka R., Nakazawa H., Takamiya M., Ohfuku Y., Funahashi T. , Tanaka T., Kudoh Y., Yamazaki J., Kushida N., Oguchi A., Aoki K., Yoshizawa T., Nakamura Y., Robb F.T., Horikoshi K., Masuchi Y., Shizuya H., Kikuchi H. DNA Res. 5:55-76(1998) [PubMed: 9679194] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: OT3.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000001 Genomic DNA. Translation: BAA29770.1.
PIR	H71113.
RefSeq	NP_142629.1.
3D structure databases
HSSP	HSSP built from PDB template 1CLF based on UniProtKB P00195.
ModBase	Search...
Genome annotation databases
GeneID	1443007.
GenomeReviews	Gene locus PH0679 in contig BA000001_GR.
KEGG	pho:PH0679.
NMPDR	fig|70601.1.peg.660.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	O58412.
Family and domain databases
InterPro	IPR001450. 4Fe4S_Fe_S_bd. IPR011898. PorD_KorD. [Graphical view]
Pfam	PF00037. Fer4. 2 hits. [Graphical view]
PRINTS	PR00353. 4FE4SFRDOXIN.
TIGRFAMs	TIGR02179. PorD_KorD. 1 hit.
PROSITE	PS00198. 4FE4S_FER_1. 2 hits. PS51379. 4FE4S_FER_2. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

VORD_PYRHO

Accession

Primary (citable) accession number: O58412

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	August 1, 1998
Last modified:	November 25, 2008
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)