O58412 (VORD_PYRHO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ketoisovalerate oxidoreductase subunit vorD
Short name=VOR
EC=1.2.7.7

Alternative name(s):
2-oxoisovalerate ferredoxin reductase subunit delta
2-oxoisovalerate oxidoreductase delta chain

Gene names

Name:	vorD
Ordered Locus Names:	PH0679

Organism

Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3) [Complete proteome] [HAMAP]

Taxonomic identifier

70601 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Thermococci › Thermococcales › Thermococcaceae › Pyrococcus

Protein attributes

Sequence length	105 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	3-methyl-2-oxobutanoate + CoA + 2 oxidized ferredoxin = S-(2-methylpropanoyl)-CoA + CO2 + 2 reduced ferredoxin + H+.
Cofactor	Binds 2 4Fe-4S clusters By similarity.
Subunit structure	Heterotetramer of one alpha, one beta, one delta and one gamma chain By similarity.
Sequence similarities	Contains 2 4Fe-4S ferredoxin-type domains.

Ontologies

Keywords
Biological process	Electron transport Transport
Domain	Repeat
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW transport Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	3-methyl-2-oxobutanoate dehydrogenase (ferredoxin) activity Inferred from electronic annotation. Source: EC 4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW electron carrier activity Inferred from electronic annotation. Source: InterPro metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 105	105	Ketoisovalerate oxidoreductase subunit vorD		PRO_0000099963
Regions
	Domain	44 – 73	30	4Fe-4S ferredoxin-type 1
	Domain	74 – 103	30	4Fe-4S ferredoxin-type 2
Sites
	Metal binding	53	1	Iron-sulfur 1 (4Fe-4S) Potential
	Metal binding	56	1	Iron-sulfur 1 (4Fe-4S) Potential
	Metal binding	59	1	Iron-sulfur 1 (4Fe-4S) Potential
	Metal binding	63	1	Iron-sulfur 2 (4Fe-4S) Potential
	Metal binding	83	1	Iron-sulfur 2 (4Fe-4S) Potential
	Metal binding	86	1	Iron-sulfur 2 (4Fe-4S) Potential
	Metal binding	89	1	Iron-sulfur 2 (4Fe-4S) Potential
	Metal binding	93	1	Iron-sulfur 1 (4Fe-4S) Potential

Sequences

	Sequence		Length	Mass (Da)
	O58412 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: 954AA31F09653FA1	FASTA	105	11,861
	10 20 30 40 50 60 MNTLFGKAKE EAKPITPKSV DEYPEAPVSL GITLVNFTGD WRTFMPVIDE SKCVKCYICW 70 80 90 100 KYCPEPAIYI KEDGFVAIDY DYCKGCGICA NECPTKAITM VREEK

References

[1]

"Complete sequence and gene organization of the genome of a hyper-thermophilic archaebacterium, Pyrococcus horikoshii OT3." Kawarabayasi Y., Sawada M., Horikawa H., Haikawa Y., Hino Y., Yamamoto S., Sekine M., Baba S., Kosugi H., Hosoyama A., Nagai Y., Sakai M., Ogura K., Otsuka R., Nakazawa H., Takamiya M., Ohfuku Y., Funahashi T. , Tanaka T., Kudoh Y., Yamazaki J., Kushida N., Oguchi A., Aoki K., Yoshizawa T., Nakamura Y., Robb F.T., Horikoshi K., Masuchi Y., Shizuya H., Kikuchi H. DNA Res. 5:55-76(1998) [PubMed: 9679194] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000001 Genomic DNA. Translation: BAA29770.1.
PIR	H71113.
RefSeq	NP_142629.1. NC_000961.1.
3D structure databases
ProteinModelPortal	O58412.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBPYRT00000000083; EBPYRP00000000083; EBPYRG00000000083.
GeneID	1443007.
GenomeReviews	Gene locus PH0679 in contig BA000001_GR.
KEGG	pho:PH0679.
NMPDR	fig|70601.1.peg.660.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000022662.
HOGENOM	HBG734156.
OMA	KCYICWK.
ProtClustDB	PRK09623.
Enzyme and pathway databases
BioCyc	PHOR70601:PH0679-MONOMER.
Family and domain databases
InterPro	IPR001450. 4Fe4S-bd_dom. IPR017896. 4Fe4S_Fe-S-bd. IPR017900. 4Fe4S_Fe_S_CS. IPR011898. PorD_KorD. [Graphical view]
KO	K00188.
Pfam	PF00037. Fer4. 2 hits. [Graphical view]
TIGRFAMs	TIGR02179. PorD_KorD. 1 hit.
PROSITE	PS00198. 4FE4S_FER_1. 2 hits. PS51379. 4FE4S_FER_2. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

VORD_PYRHO

Accession

Primary (citable) accession number: O58412

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	August 1, 1998
Last modified:	November 16, 2011
This is version 79 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program