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Protein

Aspartate racemase

Gene

PH0670

Organism
Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

L-aspartate = D-aspartate.1 Publication

Enzyme regulationi

Weakly inhibited by citrate, but not by asparagine.1 Publication

Kineticsi

  1. KM=0.7 mM for L-aspartate1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Active sitei82Proton donor/acceptor1 Publication1
    Binding sitei164SubstrateCombined sources1 Publication1
    Active sitei194Proton donor/acceptor1 Publication1

    GO - Molecular functioni

    GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Isomerase

    Enzyme and pathway databases

    BioCyciPHOR70601:GJWR-661-MONOMER.
    BRENDAi5.1.1.13. 5244.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Aspartate racemase (EC:5.1.1.131 Publication)
    Gene namesi
    Ordered Locus Names:PH0670Imported
    OrganismiPyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)Imported
    Taxonomic identifieri70601 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
    Proteomesi
    • UP000000752 Componenti: Chromosome

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Mutagenesisi82C → A: Abolishes racemase activity. 1 Publication1

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00004331351 – 228Aspartate racemaseAdd BLAST228

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Disulfide bondi73InterchainCombined sources1 Publication

    Keywords - PTMi

    Disulfide bond

    Interactioni

    Subunit structurei

    Homodimer. The existence of the interchain disulfide bond seen in the crystal structures is uncertain, but disulfide bonds have been reported for cytoplasmic proteins from thermophiles.Curated2 Publications

    Protein-protein interaction databases

    STRINGi70601.PH0670.

    Structurei

    Secondary structure

    1228
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details
    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Beta strandi4 – 8Combined sources5
    Helixi12 – 24Combined sources13
    Helixi31 – 33Combined sources3
    Beta strandi37 – 41Combined sources5
    Helixi48 – 52Combined sources5
    Helixi60 – 73Combined sources14
    Beta strandi76 – 79Combined sources4
    Helixi84 – 88Combined sources5
    Helixi89 – 95Combined sources7
    Helixi103 – 113Combined sources11
    Beta strandi117 – 122Combined sources6
    Helixi125 – 130Combined sources6
    Helixi132 – 139Combined sources8
    Beta strandi143 – 145Combined sources3
    Helixi149 – 160Combined sources12
    Helixi163 – 165Combined sources3
    Helixi168 – 184Combined sources17
    Beta strandi188 – 192Combined sources5
    Helixi195 – 200Combined sources6
    Helixi203 – 205Combined sources3
    Helixi213 – 226Combined sources14

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    1IU9X-ray2.04A103-213[»]
    1JFLX-ray1.90A/B1-228[»]
    2DX7X-ray2.00A/B1-228[»]
    ProteinModelPortaliO58403.
    SMRiO58403.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiO58403.

    Family & Domainsi

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Regioni47 – 49Substrate bindingCombined sources1 Publication3
    Regioni83 – 85Substrate bindingCombined sources1 Publication3

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiarCOG02005. Archaea.
    COG1794. LUCA.
    HOGENOMiHOG000262147.
    KOiK01779.
    OMAiHPYNVID.

    Family and domain databases

    Gene3Di3.40.50.1860. 2 hits.
    InterProiIPR015942. Asp/Glu/hydantoin_racemase.
    IPR001920. Asp/Glu_race.
    IPR018187. Asp/Glu_racemase_AS_1.
    IPR004380. Asp_race.
    [Graphical view]
    PfamiPF01177. Asp_Glu_race. 1 hit.
    [Graphical view]
    SUPFAMiSSF53681. SSF53681. 2 hits.
    TIGRFAMsiTIGR00035. asp_race. 1 hit.
    PROSITEiPS00923. ASP_GLU_RACEMASE_1. 1 hit.
    PS00924. ASP_GLU_RACEMASE_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O58403-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MKTIGILGGM GPLATAELFR RIVIKTPAKR DQEHPKVIIF NNPQIPDRTA
    60 70 80 90 100
    YILGKGEDPR PQLIWTAKRL EECGADFIIM PCNTAHAFVE DIRKAIKIPI
    110 120 130 140 150
    ISMIEETAKK VKELGFKKAG LLATTGTIVS GVYEKEFSKY GVEIMTPTED
    160 170 180 190 200
    EQKDVMRGIY EGVKAGNLKL GRELLLKTAK ILEERGAECI IAGCTEVSVV
    210 220
    LKQDDLKVPL IDPMDVIAEV AVKVALEK
    Length:228
    Mass (Da):25,158
    Last modified:August 1, 1998 - v1
    Checksum:iDDAA2CBEB39682CF
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    BA000001 Genomic DNA. Translation: BAA29761.1.
    PIRiG71112.
    RefSeqiWP_010884762.1. NC_000961.1.

    Genome annotation databases

    EnsemblBacteriaiBAA29761; BAA29761; BAA29761.
    GeneIDi1442997.
    KEGGipho:PH0670.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    BA000001 Genomic DNA. Translation: BAA29761.1.
    PIRiG71112.
    RefSeqiWP_010884762.1. NC_000961.1.

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    1IU9X-ray2.04A103-213[»]
    1JFLX-ray1.90A/B1-228[»]
    2DX7X-ray2.00A/B1-228[»]
    ProteinModelPortaliO58403.
    SMRiO58403.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    STRINGi70601.PH0670.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblBacteriaiBAA29761; BAA29761; BAA29761.
    GeneIDi1442997.
    KEGGipho:PH0670.

    Phylogenomic databases

    eggNOGiarCOG02005. Archaea.
    COG1794. LUCA.
    HOGENOMiHOG000262147.
    KOiK01779.
    OMAiHPYNVID.

    Enzyme and pathway databases

    BioCyciPHOR70601:GJWR-661-MONOMER.
    BRENDAi5.1.1.13. 5244.

    Miscellaneous databases

    EvolutionaryTraceiO58403.

    Family and domain databases

    Gene3Di3.40.50.1860. 2 hits.
    InterProiIPR015942. Asp/Glu/hydantoin_racemase.
    IPR001920. Asp/Glu_race.
    IPR018187. Asp/Glu_racemase_AS_1.
    IPR004380. Asp_race.
    [Graphical view]
    PfamiPF01177. Asp_Glu_race. 1 hit.
    [Graphical view]
    SUPFAMiSSF53681. SSF53681. 2 hits.
    TIGRFAMsiTIGR00035. asp_race. 1 hit.
    PROSITEiPS00923. ASP_GLU_RACEMASE_1. 1 hit.
    PS00924. ASP_GLU_RACEMASE_2. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Entry informationi

    Entry nameiRACD_PYRHO
    AccessioniPrimary (citable) accession number: O58403
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 27, 2015
    Last sequence update: August 1, 1998
    Last modified: November 2, 2016
    This is version 108 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.