O58403 (O58403_PYRHO) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Submitted name:
228aa long hypothetical aspartate racemase EMBL BAA29761.1

Gene names

Ordered Locus Names:

PH0670

Organism

Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3) [Complete proteome] [HAMAP] EMBL BAA29761.1

Taxonomic identifier

70601 [NCBI]

Taxonomic lineage

cellular organisms › Archaea › Euryarchaeota › Thermococci › Thermococcales › Thermococcaceae › Pyrococcus › Pyrococcus horikoshii

Protein attributes

Sequence length	228 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

Ontologies

Keywords
Technical term	3D-structure PDB 2DX7 PDB 1JFL PDB 1IU9 Complete proteome
Gene Ontology (GO)
Biological_process	cellular amino acid metabolic process Inferred from electronic annotation. Source: InterPro
Molecular_function	racemase activity, acting on amino acids and derivatives Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Active site	82	1	Proton donor/acceptor PDB 1JFL
	Active site	194	1	Proton donor/acceptor PDB 1JFL

Sequences

	Sequence		Length	Mass (Da)
	O58403 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: DDAA2CBEB39682CF	FASTA	228	25,158
	10 20 30 40 50 60 MKTIGILGGM GPLATAELFR RIVIKTPAKR DQEHPKVIIF NNPQIPDRTA YILGKGEDPR 70 80 90 100 110 120 PQLIWTAKRL EECGADFIIM PCNTAHAFVE DIRKAIKIPI ISMIEETAKK VKELGFKKAG 130 140 150 160 170 180 LLATTGTIVS GVYEKEFSKY GVEIMTPTED EQKDVMRGIY EGVKAGNLKL GRELLLKTAK 190 200 210 220 ILEERGAECI IAGCTEVSVV LKQDDLKVPL IDPMDVIAEV AVKVALEK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete sequence and gene organization of the genome of a hyper-thermophilic archaebacterium, Pyrococcus horikoshii OT3." Kawarabayasi Y., Sawada M., Horikawa H., Haikawa Y., Hino Y., Yamamoto S., Sekine M., Baba S., Kosugi H., Hosoyama A., Nagai Y., Sakai M., Ogura K., Otsuka R., Nakazawa H., Takamiya M., Ohfuku Y., Funahashi T. , Tanaka T., Kudoh Y., Yamazaki J., Kushida N., Oguchi A., Aoki K., Yoshizawa T., Nakamura Y., Robb F.T., Horikoshi K., Masuchi Y., Shizuya H., Kikuchi H. DNA Res. 5:55-76(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3.
[2]	"Structural insight into gene duplication, gene fusion and domain swapping in the evolution of PLP-independent amino acid racemases." Liu L., Iwata K., Yohda M., Miki K. FEBS Lett. 528:114-118(2002) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.04 ANGSTROMS) OF 103-213.
[3]	"Crystal structure of aspartate racemase from Pyrococcus horikoshii OT3 and its implications for molecular mechanism of PLP-independent racemization." Liu L., Iwata K., Kita A., Kawarabayasi Y., Yohda M., Miki K. J. Mol. Biol. 319:479-489(2002) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.90 ANGSTROMS), ACTIVE SITE.
[4]	"Structure of aspartate racemase complexed with a dual substrate analogue, citric acid, and implications for the reaction mechanism." Ohtaki A., Nakano Y., Iizuka R., Arakawa T., Yamada K., Odaka M., Yohda M. Proteins 70:1167-1174(2008) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.00 ANGSTROMS).

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

BA000001 Genomic DNA. Translation: BAA29761.1.

PIR

G71112.

RefSeq

NP_142620.1. NC_000961.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1IU9	X-ray	2.04	A	103-213	[»]
1JFL	X-ray	1.90	A/B	1-228	[»]
2DX7	X-ray	2.00	A/B	1-228	[»]

ProteinModelPortal

O58403.

SMR

O58403. Positions 1-228.

ModBase

Search...

Protein-protein interaction databases

STRING

70601.PH0670.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

BAA29761; BAA29761; BAA29761.

GeneID

1442997.

KEGG

pho:PH0670.

Organism-specific databases

CMR

Search...

Phylogenomic databases

eggNOG

COG1794.

HOGENOM

HOG000262147.

KO

K01779.

OMA

CNVILLG.

ProtClustDB

CLSK254788.

Enzyme and pathway databases

BioCyc

PHOR70601:GJWR-661-MONOMER.

Family and domain databases

Gene3D

3.40.50.1860. 2 hits.

InterPro

IPR015942. Asp/Glu/hydantoin_racemase.
IPR001920. Asp/Glu_race.
IPR018187. Asp/Glu_racemase_AS.
IPR004380. Asp_race.
[Graphical view]

Pfam

PF01177. Asp_Glu_race. 1 hit.
[Graphical view]

SUPFAM

SSF53681. Asp/Glu_race. 2 hits.

TIGRFAMs

TIGR00035. asp_race. 1 hit.

PROSITE

PS00923. ASP_GLU_RACEMASE_1. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

O58403.

Entry information

Entry name

O58403_PYRHO

Accession

Primary (citable) accession number: O58403

Entry history

Integrated into UniProtKB/TrEMBL:	August 1, 1998
Last sequence update:	August 1, 1998
Last modified:	May 1, 2013
This is version 82 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)