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O58403

- O58403_PYRHO

UniProt

O58403 - O58403_PYRHO

Protein
Submitted name:

228aa long hypothetical aspartate racemase

Gene

PH0670

Organism
Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Status
Unreviewed - Annotation score: 1 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 91 (01 Oct 2014)
      Sequence version 1 (01 Aug 1998)
      Previous versions | rss
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    Functioni

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei10 – 101Citrate 1; via carbonyl oxygen
    Active sitei82 – 821Proton donor/acceptorImported
    Binding sitei160 – 1601Citrate 1
    Binding sitei164 – 1641Citrate 1
    Binding sitei188 – 1881Citrate 2
    Active sitei194 – 1941Proton donor/acceptorImported
    Binding sitei195 – 1951Citrate 1; via amide nitrogen

    GO - Molecular functioni

    1. racemase activity, acting on amino acids and derivatives Source: InterPro

    GO - Biological processi

    1. cellular amino acid metabolic process Source: InterPro

    Enzyme and pathway databases

    BioCyciPHOR70601:GJWR-661-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Submitted name:
    228aa long hypothetical aspartate racemaseImported
    Gene namesi
    Ordered Locus Names:PH0670Imported
    OrganismiPyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)Imported
    Taxonomic identifieri70601 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
    ProteomesiUP000000752: Chromosome

    Interactioni

    Protein-protein interaction databases

    STRINGi70601.PH0670.

    Structurei

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1IU9X-ray2.04A103-213[»]
    1JFLX-ray1.90A/B1-228[»]
    2DX7X-ray2.00A/B1-228[»]
    ProteinModelPortaliO58403.
    SMRiO58403. Positions 1-228.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiO58403.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni47 – 493Citrate 1 binding
    Regioni83 – 853Citrate 1 binding
    Regioni115 – 1173Citrate 2 binding

    Phylogenomic databases

    eggNOGiCOG1794.
    HOGENOMiHOG000262147.
    KOiK01779.
    OMAiCNVILLG.

    Family and domain databases

    Gene3Di3.40.50.1860. 2 hits.
    InterProiIPR015942. Asp/Glu/hydantoin_racemase.
    IPR001920. Asp/Glu_race.
    IPR018187. Asp/Glu_racemase_AS.
    IPR004380. Asp_race.
    [Graphical view]
    PfamiPF01177. Asp_Glu_race. 1 hit.
    [Graphical view]
    SUPFAMiSSF53681. SSF53681. 2 hits.
    TIGRFAMsiTIGR00035. asp_race. 1 hit.
    PROSITEiPS00923. ASP_GLU_RACEMASE_1. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O58403-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKTIGILGGM GPLATAELFR RIVIKTPAKR DQEHPKVIIF NNPQIPDRTA    50
    YILGKGEDPR PQLIWTAKRL EECGADFIIM PCNTAHAFVE DIRKAIKIPI 100
    ISMIEETAKK VKELGFKKAG LLATTGTIVS GVYEKEFSKY GVEIMTPTED 150
    EQKDVMRGIY EGVKAGNLKL GRELLLKTAK ILEERGAECI IAGCTEVSVV 200
    LKQDDLKVPL IDPMDVIAEV AVKVALEK 228
    Length:228
    Mass (Da):25,158
    Last modified:August 1, 1998 - v1
    Checksum:iDDAA2CBEB39682CF
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000001 Genomic DNA. Translation: BAA29761.1.
    PIRiG71112.
    RefSeqiNP_142620.1. NC_000961.1.
    WP_010884762.1. NC_000961.1.

    Genome annotation databases

    EnsemblBacteriaiBAA29761; BAA29761; BAA29761.
    GeneIDi1442997.
    KEGGipho:PH0670.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000001 Genomic DNA. Translation: BAA29761.1 .
    PIRi G71112.
    RefSeqi NP_142620.1. NC_000961.1.
    WP_010884762.1. NC_000961.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1IU9 X-ray 2.04 A 103-213 [» ]
    1JFL X-ray 1.90 A/B 1-228 [» ]
    2DX7 X-ray 2.00 A/B 1-228 [» ]
    ProteinModelPortali O58403.
    SMRi O58403. Positions 1-228.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 70601.PH0670.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAA29761 ; BAA29761 ; BAA29761 .
    GeneIDi 1442997.
    KEGGi pho:PH0670.

    Phylogenomic databases

    eggNOGi COG1794.
    HOGENOMi HOG000262147.
    KOi K01779.
    OMAi CNVILLG.

    Enzyme and pathway databases

    BioCyci PHOR70601:GJWR-661-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei O58403.

    Family and domain databases

    Gene3Di 3.40.50.1860. 2 hits.
    InterProi IPR015942. Asp/Glu/hydantoin_racemase.
    IPR001920. Asp/Glu_race.
    IPR018187. Asp/Glu_racemase_AS.
    IPR004380. Asp_race.
    [Graphical view ]
    Pfami PF01177. Asp_Glu_race. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53681. SSF53681. 2 hits.
    TIGRFAMsi TIGR00035. asp_race. 1 hit.
    PROSITEi PS00923. ASP_GLU_RACEMASE_1. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3Imported.
    2. "Structural insight into gene duplication, gene fusion and domain swapping in the evolution of PLP-independent amino acid racemases."
      Liu L., Iwata K., Yohda M., Miki K.
      FEBS Lett. 528:114-118(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.04 ANGSTROMS) OF 103-213.
    3. "Crystal structure of aspartate racemase from Pyrococcus horikoshii OT3 and its implications for molecular mechanism of PLP-independent racemization."
      Liu L., Iwata K., Kita A., Kawarabayasi Y., Yohda M., Miki K.
      J. Mol. Biol. 319:479-489(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.90 ANGSTROMS), ACTIVE SITE.
    4. "Structure of aspartate racemase complexed with a dual substrate analogue, citric acid, and implications for the reaction mechanism."
      Ohtaki A., Nakano Y., Iizuka R., Arakawa T., Yamada K., Odaka M., Yohda M.
      Proteins 70:1167-1174(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.00 ANGSTROMS).

    Entry informationi

    Entry nameiO58403_PYRHO
    AccessioniPrimary (citable) accession number: O58403
    Entry historyi
    Integrated into UniProtKB/TrEMBL: August 1, 1998
    Last sequence update: August 1, 1998
    Last modified: October 1, 2014
    This is version 91 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiUnreviewed (UniProtKB/TrEMBL)

    Miscellaneousi

    Keywords - Technical termi

    3D-structureImported, Complete proteomeImported

    External Data

    Dasty 3