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O58403 (O58403_PYRHO) Unreviewed, UniProtKB/TrEMBL

Last modified June 11, 2014. Version 89. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein names
Gene names
Ordered Locus Names:PH0670 EMBL BAA29761.1
OrganismPyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3) [Complete proteome] [HAMAP] EMBL BAA29761.1
Taxonomic identifier70601 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus

Protein attributes

Sequence length228 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Region47 – 493Citrate 1 binding
Region83 – 853Citrate 1 binding
Region115 – 1173Citrate 2 binding

Sites

Active site821Proton donor/acceptor PDB 1JFL
Active site1941Proton donor/acceptor PDB 1JFL
Binding site101Citrate 1; via carbonyl oxygen
Binding site1601Citrate 1
Binding site1641Citrate 1
Binding site1881Citrate 2
Binding site1951Citrate 1; via amide nitrogen

Sequences

Sequence LengthMass (Da)Tools
O58403 [UniParc].

Last modified August 1, 1998. Version 1.
Checksum: DDAA2CBEB39682CF

FASTA22825,158
        10         20         30         40         50         60 
MKTIGILGGM GPLATAELFR RIVIKTPAKR DQEHPKVIIF NNPQIPDRTA YILGKGEDPR 

        70         80         90        100        110        120 
PQLIWTAKRL EECGADFIIM PCNTAHAFVE DIRKAIKIPI ISMIEETAKK VKELGFKKAG 

       130        140        150        160        170        180 
LLATTGTIVS GVYEKEFSKY GVEIMTPTED EQKDVMRGIY EGVKAGNLKL GRELLLKTAK 

       190        200        210        220 
ILEERGAECI IAGCTEVSVV LKQDDLKVPL IDPMDVIAEV AVKVALEK 

« Hide

References

« Hide 'large scale' references
[1]"Complete sequence and gene organization of the genome of a hyper-thermophilic archaebacterium, Pyrococcus horikoshii OT3."
Kawarabayasi Y., Sawada M., Horikawa H., Haikawa Y., Hino Y., Yamamoto S., Sekine M., Baba S., Kosugi H., Hosoyama A., Nagai Y., Sakai M., Ogura K., Otsuka R., Nakazawa H., Takamiya M., Ohfuku Y., Funahashi T. expand/collapse author list , Tanaka T., Kudoh Y., Yamazaki J., Kushida N., Oguchi A., Aoki K., Yoshizawa T., Nakamura Y., Robb F.T., Horikoshi K., Masuchi Y., Shizuya H., Kikuchi H.
DNA Res. 5:55-76(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3.
[2]"Structural insight into gene duplication, gene fusion and domain swapping in the evolution of PLP-independent amino acid racemases."
Liu L., Iwata K., Yohda M., Miki K.
FEBS Lett. 528:114-118(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.04 ANGSTROMS) OF 103-213.
[3]"Crystal structure of aspartate racemase from Pyrococcus horikoshii OT3 and its implications for molecular mechanism of PLP-independent racemization."
Liu L., Iwata K., Kita A., Kawarabayasi Y., Yohda M., Miki K.
J. Mol. Biol. 319:479-489(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.90 ANGSTROMS), ACTIVE SITE.
[4]"Structure of aspartate racemase complexed with a dual substrate analogue, citric acid, and implications for the reaction mechanism."
Ohtaki A., Nakano Y., Iizuka R., Arakawa T., Yamada K., Odaka M., Yohda M.
Proteins 70:1167-1174(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.00 ANGSTROMS) IN COMPLEX WITH CITRATE.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BA000001 Genomic DNA. Translation: BAA29761.1.
PIRG71112.
RefSeqNP_142620.1. NC_000961.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1IU9X-ray2.04A103-213[»]
1JFLX-ray1.90A/B1-228[»]
2DX7X-ray2.00A/B1-228[»]
ProteinModelPortalO58403.
SMRO58403. Positions 1-228.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING70601.PH0670.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAA29761; BAA29761; BAA29761.
GeneID1442997.
KEGGpho:PH0670.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1794.
HOGENOMHOG000262147.
KOK01779.
OMACNVILLG.

Enzyme and pathway databases

BioCycPHOR70601:GJWR-661-MONOMER.

Family and domain databases

Gene3D3.40.50.1860. 2 hits.
InterProIPR015942. Asp/Glu/hydantoin_racemase.
IPR001920. Asp/Glu_race.
IPR018187. Asp/Glu_racemase_AS.
IPR004380. Asp_race.
[Graphical view]
PfamPF01177. Asp_Glu_race. 1 hit.
[Graphical view]
SUPFAMSSF53681. SSF53681. 2 hits.
TIGRFAMsTIGR00035. asp_race. 1 hit.
PROSITEPS00923. ASP_GLU_RACEMASE_1. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceO58403.

Entry information

Entry nameO58403_PYRHO
AccessionPrimary (citable) accession number: O58403
Entry history
Integrated into UniProtKB/TrEMBL: August 1, 1998
Last sequence update: August 1, 1998
Last modified: June 11, 2014
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)