O58391 (SYFA_PYRHO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phenylalanine--tRNA ligase alpha chain
EC=6.1.1.20

Alternative name(s):
Phenylalanyl-tRNA synthetase alpha chain
Short name=PheRS

Gene names

Name:	pheS
Ordered Locus Names:	PH0658

Organism

Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3) [Complete proteome] [HAMAP]

Taxonomic identifier

70601 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Thermococci › Thermococcales › Thermococcaceae › Pyrococcus

Protein attributes

Sequence length	499 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + L-phenylalanyl-tRNA(Phe). HAMAP MF_00282
Cofactor	Binds 2 magnesium ions per tetramer By similarity. HAMAP MF_00282
Subunit structure	Tetramer of two alpha and two beta chains By similarity.
Subcellular location	Cytoplasm HAMAP MF_00282.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha chain type 2 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	phenylalanyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW phenylalanine-tRNA ligase activity Inferred from electronic annotation. Source: EC tRNA binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 499	499	Phenylalanine--tRNA ligase alpha chain HAMAP MF_00282		PRO_0000126817
Regions
	Compositional bias	173 – 176	4	Poly-Glu HAMAP MF_00282

Sequences

	Sequence		Length	Mass (Da)
	O58391 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: 79D782D4149F3913	FASTA	499	57,532
	10 20 30 40 50 60 MRLGYNEKLV LLKLAELKNA TVEELIEKTN LDQVAVMRAL LTLQSQGLAK VHEERRRMIK 70 80 90 100 110 120 LTETGKRYIE IGLPEIRALK ILKEKGKVTL NDLKDVLSDE ELKAIVGVLR KEGWAEVSKT 130 140 150 160 170 180 KEGLTLKLSE KGKKAEKRAI DIALEVLSKG EVSVEEIEKI ISVKELKRRK IAEEEEKVIR 190 200 210 220 230 240 NVEITDKGLE LVEKGIELKR EVSILTPELI VTGKWREVEF KPFNIKAPVK KIYPGKKQPY 250 260 270 280 290 300 RVFLDKIRRR LIEMGFIEMT VDSLIETQFW NFDALFQPQN HPAREWTDTY QLKYPEKGYL 310 320 330 340 350 360 PDENLVSKVK EAHERGLAGS RGWGYVWSPE RAMLLMPRAH ATALSARELA KGIEIPGKYF 370 380 390 400 410 420 TIQRVFRPDV LDRTHLIEFN QIDGFVASED LTFRHLLGIL KRFAIEIAGA KKVKFFPDYY 430 440 450 460 470 480 PFTEPSVQLS AYHPELGWVE FGGAGIFREE MTEALGIKVP VIAWGIGIDR LAMFKLGVDD 490 IRYLFSYDLK WLRESKLIW

References

[1]

"Complete sequence and gene organization of the genome of a hyper-thermophilic archaebacterium, Pyrococcus horikoshii OT3." Kawarabayasi Y., Sawada M., Horikawa H., Haikawa Y., Hino Y., Yamamoto S., Sekine M., Baba S., Kosugi H., Hosoyama A., Nagai Y., Sakai M., Ogura K., Otsuka R., Nakazawa H., Takamiya M., Ohfuku Y., Funahashi T. , Tanaka T., Kudoh Y., Yamazaki J., Kushida N., Oguchi A., Aoki K., Yoshizawa T., Nakamura Y., Robb F.T., Horikoshi K., Masuchi Y., Shizuya H., Kikuchi H. DNA Res. 5:55-76(1998) [PubMed: 9679194] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000001 Genomic DNA. Translation: BAA29749.1.
PIR	C71111.
RefSeq	NP_142612.1. NC_000961.1.
3D structure databases
ProteinModelPortal	O58391.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBPYRT00000001175; EBPYRP00000001175; EBPYRG00000001175.
GeneID	1442988.
GenomeReviews	Gene locus PH0658 in contig BA000001_GR.
KEGG	pho:PH0658.
NMPDR	fig|70601.1.peg.641.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000023140.
HOGENOM	HBG497668.
OMA	PEFYQCE.
PhylomeDB	O58391.
ProtClustDB	PRK04172.
Enzyme and pathway databases
BioCyc	PHOR70601:PH0658-MONOMER.
Family and domain databases
HAMAP	MF_00282. Phe_tRNA_synth_alpha2. [Tree]
InterPro	IPR006195. aa-tRNA-synth_II. IPR004529. Phe-tRNA-synth_IIc_asu. IPR022917. Phe_tRNA_synth_alpha2_bac/arc. IPR002319. Phenylalanyl-tRNA_Synthase. IPR002831. Tscrpt_reg_TrmB_N. IPR011991. WHTH_trsnscrt_rep_DNA-bd. [Graphical view]
Gene3D	G3DSA:1.10.10.10. Wing_hlx_DNA_bd. 2 hits.
KO	K01889.
Pfam	PF01978. TrmB. 1 hit. PF01409. tRNA-synt_2d. 1 hit. [Graphical view]
TIGRFAMs	TIGR00468. PheS. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYFA_PYRHO

Accession

Primary (citable) accession number: O58391

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 8, 2000
Last sequence update:	August 1, 1998
Last modified:	January 25, 2012
This is version 83 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program