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Protein

Cysteine--tRNA ligase

Gene

cysS

Organism
Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-cysteine + tRNA(Cys) = AMP + diphosphate + L-cysteinyl-tRNA(Cys).

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi29 – 291ZincBy similarity
Metal bindingi209 – 2091ZincBy similarity
Metal bindingi234 – 2341ZincBy similarity
Metal bindingi238 – 2381ZincBy similarity
Binding sitei269 – 2691ATPBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciPHOR70601:GJWR-633-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Cysteine--tRNA ligase (EC:6.1.1.16)
Alternative name(s):
Cysteinyl-tRNA synthetase
Short name:
CysRS
Gene namesi
Name:cysS
Ordered Locus Names:PH0636
OrganismiPyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Taxonomic identifieri70601 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
Proteomesi
  • UP000000752 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 476476Cysteine--tRNA ligasePRO_0000159543Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi70601.PH0636.

Structurei

3D structure databases

ProteinModelPortaliO58370.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi31 – 4111"HIGH" regionAdd
BLAST
Motifi266 – 2705"KMSKS" region

Sequence similaritiesi

Phylogenomic databases

eggNOGiarCOG00486. Archaea.
COG0215. LUCA.
HOGENOMiHOG000245250.
KOiK01883.
OMAiFDVPSMP.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_00041. Cys_tRNA_synth.
InterProiIPR015803. Cys-tRNA-ligase.
IPR015273. Cys-tRNA-synt_Ia_DALR.
IPR024909. Cys-tRNA/MSH_ligase.
IPR014729. Rossmann-like_a/b/a_fold.
IPR032678. tRNA-synt_1_cat_dom.
IPR009080. tRNAsynth_1a_anticodon-bd.
[Graphical view]
PANTHERiPTHR10890. PTHR10890. 1 hit.
PfamiPF09190. DALR_2. 1 hit.
PF01406. tRNA-synt_1e. 1 hit.
[Graphical view]
PRINTSiPR00983. TRNASYNTHCYS.
SMARTiSM00840. DALR_2. 1 hit.
[Graphical view]
SUPFAMiSSF47323. SSF47323. 1 hit.
TIGRFAMsiTIGR00435. cysS. 1 hit.

Sequencei

Sequence statusi: Complete.

O58370-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTLRIYNTLT KQKEEFKPIN EGEVRMYVCG PTVYDYPHLG HARTYIAFDV
60 70 80 90 100
IRRYLEHKGY SVLMVMNFTD IDDKIIRRAK ETGEDPAKLA EKFIKVFLED
110 120 130 140 150
MKALKVKPAD IYPRVTEHIE DIIQFIERLK EKGYAYEGSD GVYFEVQKFK
160 170 180 190 200
EYGKLSGVKL EELRKGARVE PGEGKKNPED FALWKKAKPG EPKWESPWGE
210 220 230 240 250
GRPGWHIECS VMSSKYLGES FDIHGGGNDL IFPHHENEIA QSEACFGHEW
260 270 280 290 300
VHYWLHTGFV MVKGEKMSKS LGNFVTVREL LQRYSPEVIR FFVLQKHYRS
310 320 330 340 350
PLDYSEEGLQ HAKNNLERLY NTLENIRIAM ENAELAYTWD ENDFKAYNSI
360 370 380 390 400
KEARRKFYEA MDDDFNTAEA LKAVFEVSNA INKYITEAEK PKESVLRKAW
410 420 430 440 450
EFFRTVGEIF GIFEEYFKEE KAKEEEKLIE LLIQVRAELR KERKFELADK
460 470
IREELRKLGI QLEDKGKETI WKRIKV
Length:476
Mass (Da):56,044
Last modified:August 1, 1998 - v1
Checksum:i0AC0289CBC5ED450
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000001 Genomic DNA. Translation: BAA29726.1.
PIRiD71108.
RefSeqiWP_010884735.1. NC_000961.1.

Genome annotation databases

EnsemblBacteriaiBAA29726; BAA29726; BAA29726.
GeneIDi1442970.
KEGGipho:PH0636.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000001 Genomic DNA. Translation: BAA29726.1.
PIRiD71108.
RefSeqiWP_010884735.1. NC_000961.1.

3D structure databases

ProteinModelPortaliO58370.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi70601.PH0636.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAA29726; BAA29726; BAA29726.
GeneIDi1442970.
KEGGipho:PH0636.

Phylogenomic databases

eggNOGiarCOG00486. Archaea.
COG0215. LUCA.
HOGENOMiHOG000245250.
KOiK01883.
OMAiFDVPSMP.

Enzyme and pathway databases

BioCyciPHOR70601:GJWR-633-MONOMER.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_00041. Cys_tRNA_synth.
InterProiIPR015803. Cys-tRNA-ligase.
IPR015273. Cys-tRNA-synt_Ia_DALR.
IPR024909. Cys-tRNA/MSH_ligase.
IPR014729. Rossmann-like_a/b/a_fold.
IPR032678. tRNA-synt_1_cat_dom.
IPR009080. tRNAsynth_1a_anticodon-bd.
[Graphical view]
PANTHERiPTHR10890. PTHR10890. 1 hit.
PfamiPF09190. DALR_2. 1 hit.
PF01406. tRNA-synt_1e. 1 hit.
[Graphical view]
PRINTSiPR00983. TRNASYNTHCYS.
SMARTiSM00840. DALR_2. 1 hit.
[Graphical view]
SUPFAMiSSF47323. SSF47323. 1 hit.
TIGRFAMsiTIGR00435. cysS. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3.

Entry informationi

Entry nameiSYC_PYRHO
AccessioniPrimary (citable) accession number: O58370
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: August 1, 1998
Last modified: January 20, 2016
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.