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Protein

Methionine aminopeptidase

Gene

map

Organism
Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val).UniRule annotation

Catalytic activityi

Release of N-terminal amino acids, preferentially methionine, from peptides and arylamides.UniRule annotation

Cofactori

Co2+UniRule annotation, Zn2+UniRule annotation, Mn2+UniRule annotation, Fe2+UniRule annotationNote: Binds 2 divalent metal cations per subunit. Has a high-affinity and a low affinity metal-binding site. The true nature of the physiological cofactor is under debate. The enzyme is active with cobalt, zinc, manganese or divalent iron ions. Most likely, methionine aminopeptidases function as mononuclear Fe2+-metalloproteases under physiological conditions, and the catalytically relevant metal-binding site has been assigned to the histidine-containing high-affinity site.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei62SubstrateUniRule annotation1
Metal bindingi82Divalent metal cation 1UniRule annotation1
Metal bindingi93Divalent metal cation 1UniRule annotation1
Metal bindingi93Divalent metal cation 2; catalyticUniRule annotation1
Metal bindingi153Divalent metal cation 2; catalytic; via tele nitrogenUniRule annotation1
Binding sitei161SubstrateUniRule annotation1
Metal bindingi187Divalent metal cation 2; catalyticUniRule annotation1
Metal bindingi280Divalent metal cation 1UniRule annotation1
Metal bindingi280Divalent metal cation 2; catalyticUniRule annotation1

GO - Molecular functioni

Keywordsi

Molecular functionAminopeptidase, Hydrolase, Protease
LigandMetal-binding

Enzyme and pathway databases

BioCyciPHOR70601:G1G39-615-MONOMER

Protein family/group databases

MEROPSiM24.035

Names & Taxonomyi

Protein namesi
Recommended name:
Methionine aminopeptidaseUniRule annotation (EC:3.4.11.18UniRule annotation)
Short name:
MAPUniRule annotation
Short name:
MetAPUniRule annotation
Alternative name(s):
Peptidase MUniRule annotation
Gene namesi
Name:mapUniRule annotation
Ordered Locus Names:PH0628
OrganismiPyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Taxonomic identifieri70601 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
Proteomesi
  • UP000000752 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001489791 – 295Methionine aminopeptidaseAdd BLAST295

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi70601.PH0628

Structurei

3D structure databases

ProteinModelPortaliO58362
SMRiO58362
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase M24A family. Methionine aminopeptidase archaeal type 2 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiarCOG01001 Archaea
COG0024 LUCA
HOGENOMiHOG000226277
KOiK01265
OMAiHTVLLMP
OrthoDBiPOG093Z06FJ

Family and domain databases

CDDicd01088 MetAP2, 1 hit
Gene3Di1.10.10.10, 1 hit
HAMAPiMF_01975 MetAP_2_arc, 1 hit
InterProiView protein in InterPro
IPR036005 Creatinase/aminopeptidase-like
IPR028595 MetAP_archaeal
IPR000994 Pept_M24
IPR001714 Pept_M24_MAP
IPR002468 Pept_M24A_MAP2
IPR018349 Pept_M24A_MAP2_BS
IPR036388 WH-like_DNA-bd_sf
IPR036390 WH_DNA-bd_sf
PfamiView protein in Pfam
PF00557 Peptidase_M24, 1 hit
PRINTSiPR00599 MAPEPTIDASE
SUPFAMiSSF46785 SSF46785, 1 hit
SSF55920 SSF55920, 2 hits
TIGRFAMsiTIGR00501 met_pdase_II, 1 hit
PROSITEiView protein in PROSITE
PS01202 MAP_2, 1 hit

Sequencei

Sequence statusi: Complete.

O58362-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDVDKLIEAG KIAKKVREEA VKLAKPGVSL LELAEKIESR IVELGGKPAF
60 70 80 90 100
PANLSLNEVA AHYTPYKGDQ TVLKEGDYLK IDLGVHIDGY IADTAVTVRV
110 120 130 140 150
GMDFDELMEA AKEALESAIS VARAGVEVKE LGKAIENEIR KRGFNPIVNL
160 170 180 190 200
SGHKIERYKL HAGVSIPNIY RPHDNYVLQE GDVFAIEPFA TTGAGQVIEV
210 220 230 240 250
PPTLIYMYVR DAPVRMAQAR FLLAKIKREY KTLPFAYRWL QGEMPEGQLK
260 270 280 290
LALRSLERSG ALYGYPVLRE IRGGMVTQFE HTIIVEKDSV TVTTE
Length:295
Mass (Da):32,795
Last modified:August 1, 1998 - v1
Checksum:iD228F4377CEB2AAC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000001 Genomic DNA Translation: BAA29717.1
PIRiC71107
RefSeqiWP_010884726.1, NC_000961.1

Genome annotation databases

EnsemblBacteriaiBAA29717; BAA29717; BAA29717
GeneIDi1442960
KEGGipho:PH0628

Similar proteinsi

Entry informationi

Entry nameiMAP2_PYRHO
AccessioniPrimary (citable) accession number: O58362
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: August 1, 1998
Last modified: April 25, 2018
This is version 122 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

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