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O58356 (TOP1_PYRHO) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 104. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DNA topoisomerase 1

EC=5.99.1.2
Alternative name(s):
DNA topoisomerase I
Omega-protein
Relaxing enzyme
Swivelase
Untwisting enzyme
Gene names
Name:topA
Ordered Locus Names:PH0622
OrganismPyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3) [Complete proteome] [HAMAP]
Taxonomic identifier70601 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus

Protein attributes

Sequence length686 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity. HAMAP-Rule MF_00952

Catalytic activity

ATP-independent breakage of single-stranded DNA, followed by passage and rejoining. HAMAP-Rule MF_00952

Cofactor

Magnesium. Binds two Mg2+ per subunit By similarity. HAMAP-Rule MF_00952

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00952

Sequence similarities

Belongs to the type IA topoisomerase family.

Contains 1 Toprim domain.

Ontologies

Keywords
   DomainRepeat
Zinc-finger
   LigandDNA-binding
Magnesium
Metal-binding
Zinc
   Molecular functionIsomerase
Topoisomerase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processDNA topological change

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentchromosome

Inferred from electronic annotation. Source: InterPro

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

DNA binding

Inferred from electronic annotation. Source: UniProtKB-KW

DNA topoisomerase type I activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

magnesium ion binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 686686DNA topoisomerase 1 HAMAP-Rule MF_00952
PRO_0000145181

Regions

Domain1 – 141141Toprim
Zinc finger606 – 63429C4-type 1 HAMAP-Rule MF_00952
Zinc finger653 – 67826C4-type 2; atypical HAMAP-Rule MF_00952
Region196 – 2016Interaction with DNA By similarity

Sites

Active site3171O-(5'-phospho-DNA)-tyrosine intermediate By similarity
Metal binding71Magnesium 1; catalytic By similarity
Metal binding1071Magnesium 1; catalytic By similarity
Metal binding1071Magnesium 2 By similarity
Metal binding1091Magnesium 2 By similarity
Site521Interaction with DNA By similarity
Site1661Interaction with DNA By similarity
Site1701Interaction with DNA By similarity
Site3191Interaction with DNA By similarity
Site5071Interaction with DNA By similarity

Sequences

Sequence LengthMass (Da)Tools
O58356 [UniParc].

Last modified August 1, 1998. Version 1.
Checksum: 775D9013CADDF944

FASTA68678,637
        10         20         30         40         50         60 
MILIIAEKPN VARKIAGALS ERRPIKKSLF GVPYYEIFRE GKKLIVASAV GHLYGLAPKR 

        70         80         90        100        110        120 
DVFGYPIFDI EWVPVYIAEK GKEYAREYIK ALSVLAKRVR EFIVACDYDT EGEVIGYTAL 

       130        140        150        160        170        180 
KYACGVDPRV AKRMKFSALT KRDLLNAWRN LEPTINFGMA NAGIARHILD WYWGVNLSRA 

       190        200        210        220        230        240 
LTHAIKKASG KWVVLSTGRV QGPTLKFLVE REREIQSFVP RPYWVIKLIF EKNGQKFTAN 

       250        260        270        280        290        300 
YEKDKIWEEE EGKRIVLEVK KSIPRVSNVE IKRQKRTPPH PFDLGTLQRE AYSAFGFSPK 

       310        320        330        340        350        360 
KTLDIAQSLY EKGFSSYPRT ESQKLPRNIN FRMIIQNLAK MPQYRPYAHI LLGLPELKPV 

       370        380        390        400        410        420 
EGKKEDPAHP AIYPTGEIPR PGDLTKDEEK LYDMIVRRFL AVFMEPAIRE SVKVTIRAGP 

       430        440        450        460        470        480 
HKFFLSGGRT VKKGWLSVYG KYVKFEEVTL PEFFIGERIK VIQVKREKKK TKPPARYSPA 

       490        500        510        520        530        540 
AVIKKMEDLG LGTKATRAQI LETLYQRGYI EGKKSIKVTP LGMKVIETLE KYVPEIISVE 

       550        560        570        580        590        600 
LTREFEKKME LIMEGRLTKE EVIEEAKERL TKILEEFKKR ELEIGIELAK IVVGEDEVKP 

       610        620        630        640        650        660 
LVVGKCPKCG GDLIVKYNKK TGKRFVGCSN WPKCDVTYPI LQRGEIIPTN KTCCNGAPVV 

       670        680 
IIREEDGREF EICLDINCKD WKAKSH 

« Hide

References

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BA000001 Genomic DNA. Translation: BAA29711.1.
PIRE71106.
RefSeqNP_142581.1. NC_000961.1.

3D structure databases

ProteinModelPortalO58356.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING70601.PH0622.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAA29711; BAA29711; BAA29711.
GeneID1442954.
KEGGpho:PH0622.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0551.
HOGENOMHOG000004019.
KOK03168.
OMAARFSNTE.

Enzyme and pathway databases

BioCycPHOR70601:GJWR-617-MONOMER.

Family and domain databases

Gene3D1.10.290.10. 1 hit.
1.10.460.10. 2 hits.
3.40.50.140. 1 hit.
HAMAPMF_00952. Topoisom_1_prok.
InterProIPR000380. Topo_IA.
IPR003601. Topo_IA_2.
IPR023406. Topo_IA_AS.
IPR013497. Topo_IA_cen.
IPR013824. Topo_IA_cen_sub1.
IPR013826. Topo_IA_cen_sub3.
IPR023405. Topo_IA_core_domain.
IPR003602. Topo_IA_DNA-bd.
IPR013498. Topo_IA_Znf.
IPR005739. TopoI_arch.
IPR028612. Topoisom_1_IA.
IPR006171. Toprim_domain.
[Graphical view]
PANTHERPTHR11390. PTHR11390. 1 hit.
PfamPF01131. Topoisom_bac. 1 hit.
PF01751. Toprim. 1 hit.
PF01396. zf-C4_Topoisom. 1 hit.
[Graphical view]
PRINTSPR00417. PRTPISMRASEI.
SMARTSM00437. TOP1Ac. 1 hit.
SM00436. TOP1Bc. 1 hit.
SM00493. TOPRIM. 1 hit.
[Graphical view]
SUPFAMSSF56712. SSF56712. 2 hits.
TIGRFAMsTIGR01057. topA_arch. 1 hit.
PROSITEPS00396. TOPOISOMERASE_I_PROK. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTOP1_PYRHO
AccessionPrimary (citable) accession number: O58356
Entry history
Integrated into UniProtKB/Swiss-Prot: June 20, 2001
Last sequence update: August 1, 1998
Last modified: July 9, 2014
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families