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O58328

- GLKA_PYRHO

UniProt

O58328 - GLKA_PYRHO

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Protein

ADP-dependent glucokinase

Gene

glkA

Organism
Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Catalyzes the phosphorylation of D-glucose to D-glucose 6-phosphate using ADP as the phosphate donor.

Catalytic activityi

ADP + D-glucose = AMP + D-glucose 6-phosphate.

Cofactori

Binds 1 magnesium ion per subunit.By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei33 – 331GlucoseBy similarity
Binding sitei37 – 371GlucoseBy similarity
Binding sitei91 – 911GlucoseBy similarity
Binding sitei116 – 1161GlucoseBy similarity
Binding sitei179 – 1791GlucoseBy similarity
Binding sitei200 – 2001GlucoseBy similarity
Metal bindingi269 – 2691MagnesiumBy similarity
Binding sitei295 – 2951ADPBy similarity
Metal bindingi298 – 2981MagnesiumBy similarity
Binding sitei345 – 3451ADPBy similarity
Binding sitei346 – 3461ADP; via carbonyl oxygenBy similarity
Binding sitei432 – 4321ADP; via carbonyl oxygenBy similarity
Active sitei443 – 4431Proton acceptorBy similarity
Metal bindingi443 – 4431MagnesiumBy similarity
Binding sitei443 – 4431ADPBy similarity
Binding sitei443 – 4431GlucoseBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi345 – 3473ADPBy similarity
Nucleotide bindingi441 – 4455ADPBy similarity

GO - Molecular functioni

  1. ADP-specific glucokinase activity Source: UniProtKB-EC
  2. glucokinase activity Source: UniProtKB-HAMAP
  3. magnesium ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. glucose metabolic process Source: UniProtKB-KW
  2. glycolytic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Carbohydrate metabolism, Glucose metabolism, Glycolysis

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciPHOR70601:GJWR-585-MONOMER.
UniPathwayiUPA00109.

Names & Taxonomyi

Protein namesi
Recommended name:
ADP-dependent glucokinase (EC:2.7.1.147)
Short name:
ADP-GK
Short name:
ADPGK
Gene namesi
Name:glkA
Ordered Locus Names:PH0589
OrganismiPyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Taxonomic identifieri70601 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
ProteomesiUP000000752: Chromosome

Subcellular locationi

Cytoplasm By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 457457ADP-dependent glucokinasePRO_0000184773Add
BLAST

Interactioni

Subunit structurei

Monomer.

Protein-protein interaction databases

STRINGi70601.PH0589.

Structurei

Secondary structure

1
457
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi8 – 2114
Helixi22 – 243
Beta strandi28 – 325
Beta strandi35 – 395
Helixi44 – 5411
Helixi56 – 649
Beta strandi68 – 703
Helixi73 – 8614
Beta strandi90 – 945
Helixi97 – 10610
Beta strandi109 – 1157
Helixi116 – 1249
Turni125 – 1273
Beta strandi132 – 1343
Helixi141 – 1455
Beta strandi149 – 1557
Helixi167 – 1693
Beta strandi178 – 1803
Beta strandi199 – 2057
Helixi209 – 2113
Helixi216 – 2205
Helixi222 – 2265
Beta strandi230 – 2356
Turni242 – 2443
Helixi246 – 26116
Beta strandi265 – 2695
Helixi276 – 28510
Helixi286 – 2883
Beta strandi290 – 2945
Helixi296 – 30510
Helixi309 – 3179
Beta strandi318 – 3203
Helixi323 – 33715
Beta strandi340 – 3467
Beta strandi349 – 3568
Helixi360 – 37819
Helixi384 – 3929
Helixi397 – 40913
Beta strandi416 – 4194
Beta strandi422 – 4287
Helixi443 – 45614

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1L2LX-ray2.00A1-457[»]
ProteinModelPortaliO58328.
SMRiO58328. Positions 7-457.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO58328.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini5 – 457453ADPKAdd
BLAST

Sequence similaritiesi

Belongs to the ADP-dependent glucokinase family.Curated

Phylogenomic databases

eggNOGiCOG4809.
HOGENOMiHOG000254055.
KOiK00918.
OMAiLAYNTNI.

Family and domain databases

Gene3Di3.40.1190.20. 1 hit.
HAMAPiMF_00809. ADP_glucokinase.
InterProiIPR007666. ADP_PFK/GK.
IPR015990. ADP_PFK/GK_arc.
IPR029056. Ribokinase-like.
[Graphical view]
PANTHERiPTHR21208. PTHR21208. 1 hit.
PfamiPF04587. ADP_PFK_GK. 1 hit.
[Graphical view]
PIRSFiPIRSF015883. ADP-Pfk_glckin. 1 hit.
SUPFAMiSSF53613. SSF53613. 1 hit.
PROSITEiPS51255. ADPK. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O58328-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MITMTNWESL YEKALDKVEA SIRKVRGVLL AYNTNIDAIK YLKREDLEKR
60 70 80 90 100
IEKVGKEEVL RYSEELPKEI ETIPQLLGSI LWSIKRGKAA ELLVVSREVR
110 120 130 140 150
EYMRKWGWDE LRMGGQVGIM ANLLGGVYGI PVIAHVPQLS ELQASLFLDG
160 170 180 190 200
PIYVPTFERG ELRLIHPREF RKGEEDCIHY IYEFPRNFKV LDFEAPRENR
210 220 230 240 250
FIGAADDYNP ILYVREEWIE RFEEIAKRSE LAIISGLHPL TQENHGKPIK
260 270 280 290 300
LVREHLKILN DLGIRAHLEF AFTPDEVVRL EIVKLLKHFY SVGLNEVELA
310 320 330 340 350
SVVSVMGEKE LAERIISKDP ADPIAVIEGL LKLIKETGVK RIHFHTYGYY
360 370 380 390 400
LALTREKGEH VRDALLFSAL AAATKAMKGN IEKLSDIREG LAVPIGEQGL
410 420 430 440 450
EVEKILEKEF SLRDGIGSIE DYQLTFIPTK VVKKPKSTVG IGDTISSSAF

VSEFSLH
Length:457
Mass (Da):52,090
Last modified:August 1, 1998 - v1
Checksum:iA30EF1398FA9BC94
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000001 Genomic DNA. Translation: BAA29678.1.
PIRiA71174.
RefSeqiNP_142553.1. NC_000961.1.

Genome annotation databases

EnsemblBacteriaiBAA29678; BAA29678; BAA29678.
GeneIDi1442924.
KEGGipho:PH0589.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000001 Genomic DNA. Translation: BAA29678.1 .
PIRi A71174.
RefSeqi NP_142553.1. NC_000961.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1L2L X-ray 2.00 A 1-457 [» ]
ProteinModelPortali O58328.
SMRi O58328. Positions 7-457.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 70601.PH0589.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAA29678 ; BAA29678 ; BAA29678 .
GeneIDi 1442924.
KEGGi pho:PH0589.

Phylogenomic databases

eggNOGi COG4809.
HOGENOMi HOG000254055.
KOi K00918.
OMAi LAYNTNI.

Enzyme and pathway databases

UniPathwayi UPA00109 .
BioCyci PHOR70601:GJWR-585-MONOMER.

Miscellaneous databases

EvolutionaryTracei O58328.

Family and domain databases

Gene3Di 3.40.1190.20. 1 hit.
HAMAPi MF_00809. ADP_glucokinase.
InterProi IPR007666. ADP_PFK/GK.
IPR015990. ADP_PFK/GK_arc.
IPR029056. Ribokinase-like.
[Graphical view ]
PANTHERi PTHR21208. PTHR21208. 1 hit.
Pfami PF04587. ADP_PFK_GK. 1 hit.
[Graphical view ]
PIRSFi PIRSF015883. ADP-Pfk_glckin. 1 hit.
SUPFAMi SSF53613. SSF53613. 1 hit.
PROSITEi PS51255. ADPK. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3.
  2. "Crystal structure of the ADP-dependent glucokinase from Pyrococcus horikoshii at 2.0-A resolution: a large conformational change in ADP-dependent glucokinase."
    Tsuge H., Sakuraba H., Kobe T., Kujime A., Katunuma N., Ohshima T.
    Protein Sci. 11:2456-2463(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS).
    Strain: ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3.

Entry informationi

Entry nameiGLKA_PYRHO
AccessioniPrimary (citable) accession number: O58328
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 26, 2005
Last sequence update: August 1, 1998
Last modified: October 29, 2014
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3