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O58328

- GLKA_PYRHO

UniProt

O58328 - GLKA_PYRHO

Protein

ADP-dependent glucokinase

Gene

glkA

Organism
Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 89 (01 Oct 2014)
      Sequence version 1 (01 Aug 1998)
      Previous versions | rss
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    Functioni

    Catalyzes the phosphorylation of D-glucose to D-glucose 6-phosphate using ADP as the phosphate donor.

    Catalytic activityi

    ADP + D-glucose = AMP + D-glucose 6-phosphate.

    Cofactori

    Binds 1 magnesium ion per subunit.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei33 – 331GlucoseBy similarity
    Binding sitei37 – 371GlucoseBy similarity
    Binding sitei91 – 911GlucoseBy similarity
    Binding sitei116 – 1161GlucoseBy similarity
    Binding sitei179 – 1791GlucoseBy similarity
    Binding sitei200 – 2001GlucoseBy similarity
    Metal bindingi269 – 2691MagnesiumBy similarity
    Binding sitei295 – 2951ADPBy similarity
    Metal bindingi298 – 2981MagnesiumBy similarity
    Binding sitei345 – 3451ADPBy similarity
    Binding sitei346 – 3461ADP; via carbonyl oxygenBy similarity
    Binding sitei432 – 4321ADP; via carbonyl oxygenBy similarity
    Active sitei443 – 4431Proton acceptorBy similarity
    Metal bindingi443 – 4431MagnesiumBy similarity
    Binding sitei443 – 4431ADPBy similarity
    Binding sitei443 – 4431GlucoseBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi345 – 3473ADPBy similarity
    Nucleotide bindingi441 – 4455ADPBy similarity

    GO - Molecular functioni

    1. ADP-specific glucokinase activity Source: UniProtKB-EC
    2. glucokinase activity Source: UniProtKB-HAMAP
    3. magnesium ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. glucose metabolic process Source: UniProtKB-KW
    2. glycolytic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    Carbohydrate metabolism, Glucose metabolism, Glycolysis

    Keywords - Ligandi

    Magnesium, Metal-binding

    Enzyme and pathway databases

    BioCyciPHOR70601:GJWR-585-MONOMER.
    UniPathwayiUPA00109.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    ADP-dependent glucokinase (EC:2.7.1.147)
    Short name:
    ADP-GK
    Short name:
    ADPGK
    Gene namesi
    Name:glkA
    Ordered Locus Names:PH0589
    OrganismiPyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
    Taxonomic identifieri70601 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
    ProteomesiUP000000752: Chromosome

    Subcellular locationi

    Cytoplasm By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 457457ADP-dependent glucokinasePRO_0000184773Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.

    Protein-protein interaction databases

    STRINGi70601.PH0589.

    Structurei

    Secondary structure

    1
    457
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi8 – 2114
    Helixi22 – 243
    Beta strandi28 – 325
    Beta strandi35 – 395
    Helixi44 – 5411
    Helixi56 – 649
    Beta strandi68 – 703
    Helixi73 – 8614
    Beta strandi90 – 945
    Helixi97 – 10610
    Beta strandi109 – 1157
    Helixi116 – 1249
    Turni125 – 1273
    Beta strandi132 – 1343
    Helixi141 – 1455
    Beta strandi149 – 1557
    Helixi167 – 1693
    Beta strandi178 – 1803
    Beta strandi199 – 2057
    Helixi209 – 2113
    Helixi216 – 2205
    Helixi222 – 2265
    Beta strandi230 – 2356
    Turni242 – 2443
    Helixi246 – 26116
    Beta strandi265 – 2695
    Helixi276 – 28510
    Helixi286 – 2883
    Beta strandi290 – 2945
    Helixi296 – 30510
    Helixi309 – 3179
    Beta strandi318 – 3203
    Helixi323 – 33715
    Beta strandi340 – 3467
    Beta strandi349 – 3568
    Helixi360 – 37819
    Helixi384 – 3929
    Helixi397 – 40913
    Beta strandi416 – 4194
    Beta strandi422 – 4287
    Helixi443 – 45614

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1L2LX-ray2.00A1-457[»]
    ProteinModelPortaliO58328.
    SMRiO58328. Positions 7-457.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiO58328.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini5 – 457453ADPKAdd
    BLAST

    Sequence similaritiesi

    Belongs to the ADP-dependent glucokinase family.Curated

    Phylogenomic databases

    eggNOGiCOG4809.
    HOGENOMiHOG000254055.
    KOiK00918.
    OMAiLAYNTNI.

    Family and domain databases

    Gene3Di3.40.1190.20. 1 hit.
    HAMAPiMF_00809. ADP_glucokinase.
    InterProiIPR007666. ADP_PFK/GK.
    IPR015990. ADP_PFK/GK_arc.
    IPR029056. Ribokinase-like.
    [Graphical view]
    PANTHERiPTHR21208. PTHR21208. 1 hit.
    PfamiPF04587. ADP_PFK_GK. 1 hit.
    [Graphical view]
    PIRSFiPIRSF015883. ADP-Pfk_glckin. 1 hit.
    SUPFAMiSSF53613. SSF53613. 1 hit.
    PROSITEiPS51255. ADPK. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O58328-1 [UniParc]FASTAAdd to Basket

    « Hide

    MITMTNWESL YEKALDKVEA SIRKVRGVLL AYNTNIDAIK YLKREDLEKR    50
    IEKVGKEEVL RYSEELPKEI ETIPQLLGSI LWSIKRGKAA ELLVVSREVR 100
    EYMRKWGWDE LRMGGQVGIM ANLLGGVYGI PVIAHVPQLS ELQASLFLDG 150
    PIYVPTFERG ELRLIHPREF RKGEEDCIHY IYEFPRNFKV LDFEAPRENR 200
    FIGAADDYNP ILYVREEWIE RFEEIAKRSE LAIISGLHPL TQENHGKPIK 250
    LVREHLKILN DLGIRAHLEF AFTPDEVVRL EIVKLLKHFY SVGLNEVELA 300
    SVVSVMGEKE LAERIISKDP ADPIAVIEGL LKLIKETGVK RIHFHTYGYY 350
    LALTREKGEH VRDALLFSAL AAATKAMKGN IEKLSDIREG LAVPIGEQGL 400
    EVEKILEKEF SLRDGIGSIE DYQLTFIPTK VVKKPKSTVG IGDTISSSAF 450
    VSEFSLH 457
    Length:457
    Mass (Da):52,090
    Last modified:August 1, 1998 - v1
    Checksum:iA30EF1398FA9BC94
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000001 Genomic DNA. Translation: BAA29678.1.
    PIRiA71174.
    RefSeqiNP_142553.1. NC_000961.1.
    WP_010884690.1. NC_000961.1.

    Genome annotation databases

    EnsemblBacteriaiBAA29678; BAA29678; BAA29678.
    GeneIDi1442924.
    KEGGipho:PH0589.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000001 Genomic DNA. Translation: BAA29678.1 .
    PIRi A71174.
    RefSeqi NP_142553.1. NC_000961.1.
    WP_010884690.1. NC_000961.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1L2L X-ray 2.00 A 1-457 [» ]
    ProteinModelPortali O58328.
    SMRi O58328. Positions 7-457.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 70601.PH0589.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAA29678 ; BAA29678 ; BAA29678 .
    GeneIDi 1442924.
    KEGGi pho:PH0589.

    Phylogenomic databases

    eggNOGi COG4809.
    HOGENOMi HOG000254055.
    KOi K00918.
    OMAi LAYNTNI.

    Enzyme and pathway databases

    UniPathwayi UPA00109 .
    BioCyci PHOR70601:GJWR-585-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei O58328.

    Family and domain databases

    Gene3Di 3.40.1190.20. 1 hit.
    HAMAPi MF_00809. ADP_glucokinase.
    InterProi IPR007666. ADP_PFK/GK.
    IPR015990. ADP_PFK/GK_arc.
    IPR029056. Ribokinase-like.
    [Graphical view ]
    PANTHERi PTHR21208. PTHR21208. 1 hit.
    Pfami PF04587. ADP_PFK_GK. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF015883. ADP-Pfk_glckin. 1 hit.
    SUPFAMi SSF53613. SSF53613. 1 hit.
    PROSITEi PS51255. ADPK. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3.
    2. "Crystal structure of the ADP-dependent glucokinase from Pyrococcus horikoshii at 2.0-A resolution: a large conformational change in ADP-dependent glucokinase."
      Tsuge H., Sakuraba H., Kobe T., Kujime A., Katunuma N., Ohshima T.
      Protein Sci. 11:2456-2463(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS).
      Strain: ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3.

    Entry informationi

    Entry nameiGLKA_PYRHO
    AccessioniPrimary (citable) accession number: O58328
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 26, 2005
    Last sequence update: August 1, 1998
    Last modified: October 1, 2014
    This is version 89 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3