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Protein

ADP-dependent glucokinase

Gene

glkA

Organism
Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the phosphorylation of D-glucose to D-glucose 6-phosphate using ADP as the phosphate donor.

Catalytic activityi

ADP + D-glucose = AMP + D-glucose 6-phosphate.

Cofactori

Mg2+By similarityNote: Binds 1 Mg2+ ion per subunit.By similarity

Pathwayi: glycolysis

This protein is involved in the pathway glycolysis, which is part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei33GlucoseBy similarity1
Binding sitei37GlucoseBy similarity1
Binding sitei91GlucoseBy similarity1
Binding sitei116GlucoseBy similarity1
Binding sitei179GlucoseBy similarity1
Binding sitei200GlucoseBy similarity1
Metal bindingi269MagnesiumBy similarity1
Binding sitei295ADPBy similarity1
Metal bindingi298MagnesiumBy similarity1
Binding sitei345ADPBy similarity1
Binding sitei346ADP; via carbonyl oxygenBy similarity1
Binding sitei432ADP; via carbonyl oxygenBy similarity1
Active sitei443Proton acceptorBy similarity1
Metal bindingi443MagnesiumBy similarity1
Binding sitei443ADPBy similarity1
Binding sitei443GlucoseBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi345 – 347ADPBy similarity3
Nucleotide bindingi441 – 445ADPBy similarity5

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Carbohydrate metabolism, Glucose metabolism, Glycolysis

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00109.

Names & Taxonomyi

Protein namesi
Recommended name:
ADP-dependent glucokinase (EC:2.7.1.147)
Short name:
ADP-GK
Short name:
ADPGK
Gene namesi
Name:glkA
Ordered Locus Names:PH0589
OrganismiPyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Taxonomic identifieri70601 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
Proteomesi
  • UP000000752 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001847731 – 457ADP-dependent glucokinaseAdd BLAST457

Interactioni

Subunit structurei

Monomer.

Protein-protein interaction databases

STRINGi70601.PH0589.

Structurei

Secondary structure

1457
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi8 – 21Combined sources14
Helixi22 – 24Combined sources3
Beta strandi28 – 32Combined sources5
Beta strandi35 – 39Combined sources5
Helixi44 – 54Combined sources11
Helixi56 – 64Combined sources9
Beta strandi68 – 70Combined sources3
Helixi73 – 86Combined sources14
Beta strandi90 – 94Combined sources5
Helixi97 – 106Combined sources10
Beta strandi109 – 115Combined sources7
Helixi116 – 124Combined sources9
Turni125 – 127Combined sources3
Beta strandi132 – 134Combined sources3
Helixi141 – 145Combined sources5
Beta strandi149 – 155Combined sources7
Helixi167 – 169Combined sources3
Beta strandi178 – 180Combined sources3
Beta strandi199 – 205Combined sources7
Helixi209 – 211Combined sources3
Helixi216 – 220Combined sources5
Helixi222 – 226Combined sources5
Beta strandi230 – 235Combined sources6
Turni242 – 244Combined sources3
Helixi246 – 261Combined sources16
Beta strandi265 – 269Combined sources5
Helixi276 – 285Combined sources10
Helixi286 – 288Combined sources3
Beta strandi290 – 294Combined sources5
Helixi296 – 305Combined sources10
Helixi309 – 317Combined sources9
Beta strandi318 – 320Combined sources3
Helixi323 – 337Combined sources15
Beta strandi340 – 346Combined sources7
Beta strandi349 – 356Combined sources8
Helixi360 – 378Combined sources19
Helixi384 – 392Combined sources9
Helixi397 – 409Combined sources13
Beta strandi416 – 419Combined sources4
Beta strandi422 – 428Combined sources7
Helixi443 – 456Combined sources14

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1L2LX-ray2.00A1-457[»]
ProteinModelPortaliO58328.
SMRiO58328.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO58328.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini5 – 457ADPKAdd BLAST453

Sequence similaritiesi

Belongs to the ADP-dependent glucokinase family.Curated

Phylogenomic databases

eggNOGiarCOG03371. Archaea.
COG4809. LUCA.
HOGENOMiHOG000254055.
KOiK00918.
OMAiLAYNTNI.

Family and domain databases

Gene3Di3.40.1190.20. 1 hit.
HAMAPiMF_00809. ADP_glucokinase. 1 hit.
InterProiIPR031299. ADP_GK.
IPR007666. ADP_PFK/GK.
IPR015990. ADP_PFK/GK_arc.
IPR029056. Ribokinase-like.
[Graphical view]
PANTHERiPTHR21208. PTHR21208. 1 hit.
PfamiPF04587. ADP_PFK_GK. 1 hit.
[Graphical view]
PIRSFiPIRSF015883. ADP-Pfk_glckin. 1 hit.
SUPFAMiSSF53613. SSF53613. 1 hit.
PROSITEiPS51255. ADPK. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O58328-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MITMTNWESL YEKALDKVEA SIRKVRGVLL AYNTNIDAIK YLKREDLEKR
60 70 80 90 100
IEKVGKEEVL RYSEELPKEI ETIPQLLGSI LWSIKRGKAA ELLVVSREVR
110 120 130 140 150
EYMRKWGWDE LRMGGQVGIM ANLLGGVYGI PVIAHVPQLS ELQASLFLDG
160 170 180 190 200
PIYVPTFERG ELRLIHPREF RKGEEDCIHY IYEFPRNFKV LDFEAPRENR
210 220 230 240 250
FIGAADDYNP ILYVREEWIE RFEEIAKRSE LAIISGLHPL TQENHGKPIK
260 270 280 290 300
LVREHLKILN DLGIRAHLEF AFTPDEVVRL EIVKLLKHFY SVGLNEVELA
310 320 330 340 350
SVVSVMGEKE LAERIISKDP ADPIAVIEGL LKLIKETGVK RIHFHTYGYY
360 370 380 390 400
LALTREKGEH VRDALLFSAL AAATKAMKGN IEKLSDIREG LAVPIGEQGL
410 420 430 440 450
EVEKILEKEF SLRDGIGSIE DYQLTFIPTK VVKKPKSTVG IGDTISSSAF

VSEFSLH
Length:457
Mass (Da):52,090
Last modified:August 1, 1998 - v1
Checksum:iA30EF1398FA9BC94
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000001 Genomic DNA. Translation: BAA29678.1.
PIRiA71174.

Genome annotation databases

EnsemblBacteriaiBAA29678; BAA29678; BAA29678.
KEGGipho:PH0589.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000001 Genomic DNA. Translation: BAA29678.1.
PIRiA71174.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1L2LX-ray2.00A1-457[»]
ProteinModelPortaliO58328.
SMRiO58328.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi70601.PH0589.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAA29678; BAA29678; BAA29678.
KEGGipho:PH0589.

Phylogenomic databases

eggNOGiarCOG03371. Archaea.
COG4809. LUCA.
HOGENOMiHOG000254055.
KOiK00918.
OMAiLAYNTNI.

Enzyme and pathway databases

UniPathwayiUPA00109.

Miscellaneous databases

EvolutionaryTraceiO58328.

Family and domain databases

Gene3Di3.40.1190.20. 1 hit.
HAMAPiMF_00809. ADP_glucokinase. 1 hit.
InterProiIPR031299. ADP_GK.
IPR007666. ADP_PFK/GK.
IPR015990. ADP_PFK/GK_arc.
IPR029056. Ribokinase-like.
[Graphical view]
PANTHERiPTHR21208. PTHR21208. 1 hit.
PfamiPF04587. ADP_PFK_GK. 1 hit.
[Graphical view]
PIRSFiPIRSF015883. ADP-Pfk_glckin. 1 hit.
SUPFAMiSSF53613. SSF53613. 1 hit.
PROSITEiPS51255. ADPK. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGLKA_PYRHO
AccessioniPrimary (citable) accession number: O58328
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 26, 2005
Last sequence update: August 1, 1998
Last modified: November 2, 2016
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.