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Protein

Glycerate 2-kinase

Gene

gck

Organism
Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the ATP-dependent phosphorylation of D-glycerate to 2-phosphoglycerate. It can also utilize GTP, CTP, UTP, ADP or pyrophosphate as phosphate donor.1 Publication

Catalytic activityi

ATP + D-glycerate = ADP + 2-phospho-D-glycerate.

Cofactori

Mg2+1 Publication, Ni2+1 Publication, Mn2+1 Publication, Co2+1 PublicationNote: Magnesium. It could be replaced to some extent by nickel, manganese or cobalt.1 Publication

Kineticsi

  1. KM=44 µM for glycerate (at 45 degrees Celsius and at pH 7.0)1 Publication
  2. KM=102 µM for ATP (at 45 degrees Celsius and at pH 7.0)1 Publication
  1. Vmax=624 µmol/min/mg enzyme with glycerate as substrate (at 45 degrees Celsius and at pH 7.0)1 Publication
  2. Vmax=639 µmol/min/mg enzyme with ATP as substrate (at 45 degrees Celsius and at pH 7.0)1 Publication

pH dependencei

Optimum pH is 7.0 and half of the maximum activity remains at pH 6-10.1 Publication

Temperature dependencei

Optimum temperature is 45 degrees Celsius. It has strong activity at moderate temperature 30-50 degrees Celsius and about half of the maximal activity is retained at 100 degrees Celsius.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei58SubstrateBy similarity1

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • glycerate 2-kinase activity Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.1.165. 5244.

Names & Taxonomyi

Protein namesi
Recommended name:
Glycerate 2-kinase (EC:2.7.1.165)
Short name:
GCK
Alternative name(s):
2-phosphoglycerate forming glycerate kinase
Gene namesi
Name:gck
Ordered Locus Names:PH0495
OrganismiPyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Taxonomic identifieri70601 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
Proteomesi
  • UP000000752 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004151501 – 440Glycerate 2-kinaseAdd BLAST440

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

STRINGi70601.PH0495.

Structurei

Secondary structure

1440
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi6 – 20Combined sources15
Helixi23 – 30Combined sources8
Beta strandi31 – 33Combined sources3
Beta strandi35 – 40Combined sources6
Beta strandi43 – 46Combined sources4
Beta strandi51 – 57Combined sources7
Helixi60 – 70Combined sources11
Beta strandi74 – 81Combined sources8
Beta strandi89 – 96Combined sources8
Beta strandi98 – 100Combined sources3
Helixi103 – 118Combined sources16
Beta strandi123 – 129Combined sources7
Helixi133 – 136Combined sources4
Helixi146 – 158Combined sources13
Helixi163 – 171Combined sources9
Beta strandi174 – 176Combined sources3
Turni177 – 179Combined sources3
Helixi180 – 183Combined sources4
Beta strandi186 – 194Combined sources9
Turni202 – 204Combined sources3
Helixi205 – 207Combined sources3
Helixi217 – 226Combined sources10
Turni230 – 232Combined sources3
Helixi235 – 245Combined sources11
Beta strandi260 – 266Combined sources7
Helixi268 – 281Combined sources14
Beta strandi285 – 293Combined sources9
Helixi297 – 314Combined sources18
Beta strandi320 – 328Combined sources9
Beta strandi335 – 337Combined sources3
Helixi343 – 351Combined sources9
Turni352 – 357Combined sources6
Beta strandi358 – 366Combined sources9
Beta strandi372 – 375Combined sources4
Beta strandi378 – 382Combined sources5
Helixi385 – 391Combined sources7
Helixi396 – 401Combined sources6
Helixi405 – 411Combined sources7
Beta strandi427 – 435Combined sources9

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1X3LX-ray2.10A1-440[»]
ProteinModelPortaliO58231.
SMRiO58231.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO58231.

Family & Domainsi

Sequence similaritiesi

Belongs to the glycerate kinase type-1 family.Curated

Phylogenomic databases

eggNOGiarCOG04170. Archaea.
COG2379. LUCA.
HOGENOMiHOG000018914.
KOiK11529.
OMAiTHTNVND.

Family and domain databases

Gene3Di3.40.1480.10. 1 hit.
InterProiIPR007835. MOFRL.
IPR025286. MOFRL_assoc_dom.
[Graphical view]
PfamiPF13660. DUF4147. 1 hit.
PF05161. MOFRL. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O58231-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIAMDIREIG LRLVGEAIKA ADPYRAVLNA VKVSDDKIIV QGKEFEIKGK
60 70 80 90 100
VYVIALGKAA CEMARAIEDI LDVEDGVAVT KYGYGKELKR IKVIEAGHPI
110 120 130 140 150
PDEKSILGAK EALSILNRAR ENDIVFILIS GGGSALFELP EEGISLEDLK
160 170 180 190 200
LTTDLLLKSG AKIHEINTVR KHISKVKGGK LAKMIKGTGI VLIISDVVGD
210 220 230 240 250
NLEAIASGPT VKDPTTFEDA KRILELYDIW EKVPESVRLH IERGLRGEVE
260 270 280 290 300
ETLKEDLPNV HNFLIASNSI SCEAIAREAQ RLGFKAYIMT TTLEGEAKDA
310 320 330 340 350
GLFIGSIVQE IAERGRPFEP PVVLVFGGET TVTIEGKGGK GGPNQEIALS
360 370 380 390 400
ATRKISDLEA LIVAFDTDGT DGPTDAAGGI VDGTTYKKLR EKGIDVEKVL
410 420 430 440
KEHNSYEALK KVGGLLFTGP TGTNVNSIVI AIVTSKRGRT
Length:440
Mass (Da):47,375
Last modified:August 1, 1998 - v1
Checksum:iC9C3E4FE4207F4B1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000001 Genomic DNA. Translation: BAA29583.1.
PIRiB71162.

Genome annotation databases

EnsemblBacteriaiBAA29583; BAA29583; BAA29583.
KEGGipho:PH0495.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000001 Genomic DNA. Translation: BAA29583.1.
PIRiB71162.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1X3LX-ray2.10A1-440[»]
ProteinModelPortaliO58231.
SMRiO58231.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi70601.PH0495.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAA29583; BAA29583; BAA29583.
KEGGipho:PH0495.

Phylogenomic databases

eggNOGiarCOG04170. Archaea.
COG2379. LUCA.
HOGENOMiHOG000018914.
KOiK11529.
OMAiTHTNVND.

Enzyme and pathway databases

BRENDAi2.7.1.165. 5244.

Miscellaneous databases

EvolutionaryTraceiO58231.

Family and domain databases

Gene3Di3.40.1480.10. 1 hit.
InterProiIPR007835. MOFRL.
IPR025286. MOFRL_assoc_dom.
[Graphical view]
PfamiPF13660. DUF4147. 1 hit.
PF05161. MOFRL. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGCK_PYRHO
AccessioniPrimary (citable) accession number: O58231
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 25, 2012
Last sequence update: August 1, 1998
Last modified: November 2, 2016
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.